Rh4CG416900
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004713
Physical Location & Seq
Reverse (-)
66205221 .. 66208513
3293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG416900.1

Sequence Viewer

Length: 1374 bp
ATGGAACCAGCTCACACATTGTTCATTGGCCCCAGTGCCTCAGGAAACATTGTCTATGATGACATTGGCATTCTTGATGAGCTTGAAAGCTTTGACATAGAAGAAGACAATGAGGTGGACGTCTTTGAGATTCCGTCATGGACAAGTGAACGTGGTAGCAAGGTTCTTGTTAATGTGGATAGCTTTGGTGCTACTGGAGATGGAGTTTCTGATGATACTCAGGCTTTTGTAAAAGCTTGGGATATTGCATGCAATACAACAAAAGCTGTTTTCCTGGTACCCCAAGGACGCCGCTATCTAGTAAATGCAACAAGATTTGAAGGGCCATGTGCAGATAGCTTAGTCATCCAGATTGAGGGAACAATCGTAGCACCAGATGATCCTAACAACTGGGATCCAAAATTTCCACGAACATGGCTTGACTTCTATAAATTGAATGGAGTACTTTTCCAAGGGCATGGAGTTATTGATGGATCAGGCAGTAAATGGTGGGCTTCATCCTGCAAAAAGAATAAGACAAATCCTTGCAGGAGTGCTCCTACAGCATTTACTATTGATAGGAGCTCAGCTATAAAGATAAAAGGGCTCACCATCCAGAACAGCCAACAAATGCATTTTACTATCTCTCAGAGCGACTCCGTACGAGTTTATGCAGTCCAAGTCTCGGCTCCAGGAGACAGTCCTAACACTGATGGAATCCATATCACTGCATCTACTAATGTGATTCTCCAAGACTGCAAAATTGGAACAGGAGATGACTGCATCTCAATTGTCAATGCTACATCAAATATTAAGATGAAGAGAATCTTTTGCGGACCAGGACATGGAGTCAGCATCGGAAGTCTAGGGAAGGACAACTCCAGCGGCATAGTGACAAAAATTGTCTTGGATACAGCATACCTCAAGGAGACTACCAATGGCCTCCGCATTAAGACTTGGCAGGGAGGTTCTGGTTATGTTCGTGGGGTCCGTTTCCAAAACGTAAGGATGGAAGATGTAGCAAATCCCATAATCATAGATCAGTTTTACTGTGACTCCCCCAAAGCTTGTCAAAATCAGACATCAGCTGTGCAAATAAGCCAAATCATGTATCGGAACATCTCGGGAATCACAACGAGTAAGAATGCCATGAAGTTTGCCTGCAGTGACACAGTTCCATGCAGCAACCTAGTCCTCAGTAATGTCAACCTGGAGAAGAAGGACGGCACTGTGGAGACATATTGCAACTCTGCCGAAGGCTTCGGGACTGGAGTTGTGCATCCATCAGCAGATTGTCTGAATTCTCATGACAAAGACTTGACAATCATCAATCAGCCTACAGAAGTTAAAGATGCAGATCTCAGCATAGAAGATATTGTGCATACTGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

457

Amino Acids

49.59

Weight (kDa)

4.94

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 86 - 410 6.5e-91 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
Acc65I GGTACC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 277
AccB7I CCANNNNNTGG 1 cut(s) 824
AciI CCGC 4 cut(s) 292, 813, 864, 925
AclWI GGATC 4 cut(s) 374, 389, 402, 481
AcsI RAATTY 2 cut(s) 401, 1279
AcyI GRCGYC 2 cut(s) 120, 289
AfaI GTAC 3 cut(s) 279, 444, 642
AfiI CCNNNNNNNGG 3 cut(s) 355, 664, 824
AgsI TTSAA 3 cut(s) 86, 320, 436
AhdI GACNNNNNGTC 1 cut(s) 827
AjnI CCWGG 4 cut(s) 273, 670, 817, 1188
Alw21I GWGCWC 2 cut(s) 538, 566
Alw26I GTCTC 4 cut(s) 667, 669, 902, 1208
AlwI GGATC 4 cut(s) 374, 389, 402, 481
Ama87I CYCGRG 1 cut(s) 1102
AoxI GGCC 3 cut(s) 28, 323, 919
ApeKI GCWGC 1 cut(s) 1161
ApoI RAATTY 2 cut(s) 401, 1279
Asp718I GGTACC 1 cut(s) 277
AspS9I GGNCC 4 cut(s) 29, 323, 815, 967
AsuHPI GGTGA 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 1102
AvaII GGWCC 2 cut(s) 815, 967
AxyI CCTNAGG 1 cut(s) 40
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 566, 588
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 2 cut(s) 538, 566
BbvI GCAGC 1 cut(s) 1173
BccI CCATC 6 cut(s) 194, 464, 599, 686, 982, 1270
BceAI ACGGC 1 cut(s) 1219
BciT130I CCWGG 4 cut(s) 275, 672, 819, 1190
BciVI GTATCC 1 cut(s) 883
BcoDI GTCTC 4 cut(s) 667, 669, 902, 1208
BfaI CTAG 3 cut(s) 299, 845, 1169
BfmI CTRYAG 3 cut(s) 540, 1141, 1317
BfuI GTATCC 1 cut(s) 883
BglII AGATCT 1 cut(s) 1336
BisI GCNGC 3 cut(s) 292, 865, 1162
BlpI GCTNAGC 1 cut(s) 565
BlsI GCNGC 3 cut(s) 293, 866, 1163
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 4 cut(s) 275, 672, 819, 1190
Bme18I GGWCC 2 cut(s) 815, 967
BmeRI GACNNNNNGTC 1 cut(s) 827
BmeT110I CYCGRG 1 cut(s) 1102
BmgT120I GGNCC 4 cut(s) 29, 323, 815, 967
BmiI GGNNCC 6 cut(s) 6, 31, 279, 396, 669, 968
BmrFI CCNGG 4 cut(s) 275, 672, 819, 1190
BmrI ACTGGG 2 cut(s) 27, 400
BmsI GCATC 5 cut(s) 719, 771, 843, 1267, 1321
BmuI ACTGGG 2 cut(s) 27, 400
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 5 cut(s) 216, 654, 844, 1211, 1269
Bpu1102I GCTNAGC 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 1335
BsaHI GRCGYC 2 cut(s) 120, 289
BsaJI CCNNGG 2 cut(s) 283, 451
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 664, 824
Bse1I ACTGG 4 cut(s) 33, 199, 395, 1252
Bse21I CCTNAGG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 1335
BseBI CCWGG 4 cut(s) 275, 672, 819, 1190
BseDI CCNNGG 2 cut(s) 283, 451
BseGI GGATG 5 cut(s) 345, 497, 591, 993, 1258
BseJI GATNNNNATC 1 cut(s) 1335
BseLI CCNNNNNNNGG 3 cut(s) 355, 664, 824
BseMII CTCAG 6 cut(s) 54, 233, 579, 641, 1189, 1354
BseNI ACTGG 4 cut(s) 33, 199, 395, 1252
BseXI GCAGC 1 cut(s) 1173
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 3 cut(s) 30, 325, 921
BshNI GGYRCC 1 cut(s) 277
BsiHKAI GWGCWC 2 cut(s) 538, 566
BsiHKCI CYCGRG 1 cut(s) 1102
BsiWI CGTACG 1 cut(s) 640
BslFI GGGAC 1 cut(s) 1258
BslI CCNNNNNNNGG 3 cut(s) 355, 664, 824
BsmAI GTCTC 4 cut(s) 667, 669, 902, 1208
BsmFI GGGAC 1 cut(s) 1258
BsmI GAATGC 2 cut(s) 69, 1129
BsnI GGCC 3 cut(s) 30, 325, 921
BsoBI CYCGRG 1 cut(s) 1102
Bsp1286I GDGCHC 3 cut(s) 538, 566, 588
Bsp143I GATC 5 cut(s) 379, 394, 473, 1018, 1336
Bsp1720I GCTNAGC 1 cut(s) 565
BspACI CCGC 4 cut(s) 292, 813, 864, 925
BspANI GGCC 3 cut(s) 30, 325, 921
BspCNI CTCAG 6 cut(s) 53, 232, 578, 640, 1188, 1353
BspHI TCATGA 1 cut(s) 1285
BspLI GGNNCC 6 cut(s) 6, 31, 279, 396, 669, 968
BspMAI CTGCAG 1 cut(s) 1145
BspPI GGATC 4 cut(s) 374, 389, 402, 481
BspT107I GGYRCC 1 cut(s) 277
BsrI ACTGG 4 cut(s) 33, 199, 395, 1252
BssECI CCNNGG 2 cut(s) 283, 451
BssMI GATC 5 cut(s) 379, 394, 473, 1018, 1336
BssNI GRCGYC 2 cut(s) 120, 289
BssT1I CCWWGG 2 cut(s) 283, 451
Bst2UI CCWGG 4 cut(s) 275, 672, 819, 1190
Bst4CI ACNGT 4 cut(s) 680, 1031, 1153, 1210
Bst6I CTCTTC 1 cut(s) 794
BstACI GRCGYC 2 cut(s) 120, 289
BstC8I GCNNGC 2 cut(s) 250, 1141
BstDEI CTNAG 7 cut(s) 40, 219, 340, 565, 627, 1175, 1340
BstF5I GGATG 5 cut(s) 345, 497, 591, 993, 1258
BstKTI GATC 5 cut(s) 382, 397, 476, 1021, 1339
BstMAI GTCTC 4 cut(s) 667, 669, 902, 1208
BstMBI GATC 5 cut(s) 379, 394, 473, 1018, 1336
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 4 cut(s) 275, 672, 819, 1190
BstNSI RCATGY 1 cut(s) 252
BstSCI CCNGG 4 cut(s) 273, 670, 817, 1188
BstSFI CTRYAG 3 cut(s) 540, 1141, 1317
BstV1I GCAGC 1 cut(s) 1173
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 394, 1336
BstXI CCANNNNNNTGG 2 cut(s) 414, 458
BstYI RGATCY 2 cut(s) 394, 1336
Bsu36I CCTNAGG 1 cut(s) 40
BsuI GTATCC 1 cut(s) 883
BsuRI GGCC 3 cut(s) 30, 325, 921
BtsCI GGATG 5 cut(s) 345, 497, 591, 993, 1258
BtsI GCAGTG 2 cut(s) 705, 1150
BtsIMutI CAGTG 5 cut(s) 40, 687, 705, 1150, 1206
Cac8I GCNNGC 2 cut(s) 250, 1141
CciI TCATGA 1 cut(s) 1285
Cfr13I GGNCC 4 cut(s) 29, 323, 815, 967
CseI GACGC 1 cut(s) 297
Csp6I GTAC 3 cut(s) 278, 443, 641
CviQI GTAC 3 cut(s) 278, 443, 641
DdeI CTNAG 7 cut(s) 40, 219, 340, 565, 627, 1175, 1340
DpnI GATC 5 cut(s) 381, 396, 475, 1020, 1338
DpnII GATC 5 cut(s) 379, 394, 473, 1018, 1336
DriI GACNNNNNGTC 1 cut(s) 827
Eam1104I CTCTTC 1 cut(s) 794
Eam1105I GACNNNNNGTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 794
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 2 cut(s) 283, 451
Eco24I GRGCYC 2 cut(s) 566, 588
Eco47I GGWCC 2 cut(s) 815, 967
Eco53kI GAGCTC 1 cut(s) 564
Eco81I CCTNAGG 1 cut(s) 40
Eco88I CYCGRG 1 cut(s) 1102
EcoICRI GAGCTC 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 1279
EcoRII CCWGG 4 cut(s) 273, 670, 817, 1188
EcoT14I CCWWGG 2 cut(s) 283, 451
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 2 cut(s) 566, 588
ErhI CCWWGG 2 cut(s) 283, 451
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FaqI GGGAC 1 cut(s) 1258
Fnu4HI GCNGC 3 cut(s) 292, 865, 1162
FokI GGATG 5 cut(s) 332, 484, 578, 1000, 1245
FriOI GRGCYC 2 cut(s) 566, 588
Fsp4HI GCNGC 3 cut(s) 292, 865, 1162
FspBI CTAG 3 cut(s) 299, 845, 1169
GluI GCNGC 3 cut(s) 292, 865, 1162
GsuI CTGGAG 5 cut(s) 216, 654, 844, 1211, 1269
HaeIII GGCC 3 cut(s) 30, 325, 921
HgaI GACGC 1 cut(s) 297
Hin1I GRCGYC 2 cut(s) 120, 289
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HindIII AAGCTT 3 cut(s) 88, 234, 1044
HinfI GANTC 8 cut(s) 130, 635, 696, 724, 804, 828, 1034, 1107
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 3 cut(s) 118, 149, 1186
Hpy188I TCNGA 7 cut(s) 211, 630, 839, 1059, 1095, 1278, 1373
Hpy188III TCNNGA 7 cut(s) 42, 74, 349, 595, 1104, 1243, 1286
Hpy8I GTNNAC 3 cut(s) 118, 149, 1186
HpyAV CCTTC 4 cut(s) 314, 844, 1192, 1229
HpyCH4III ACNGT 4 cut(s) 680, 1031, 1153, 1210
HpyCH4IV ACGT 3 cut(s) 120, 151, 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 7 cut(s) 40, 219, 340, 565, 627, 1175, 1340
HpySE526I ACGT 3 cut(s) 120, 151, 981
Hsp92I GRCGYC 2 cut(s) 120, 289
KpnI GGTACC 1 cut(s) 281
Kzo9I GATC 5 cut(s) 379, 394, 473, 1018, 1336
LmnI GCTCC 3 cut(s) 541, 561, 673
Lsp1109I GCAGC 1 cut(s) 1173
LweI GCATC 5 cut(s) 719, 771, 843, 1267, 1321
MaeI CTAG 3 cut(s) 299, 845, 1169
MaeII ACGT 3 cut(s) 120, 151, 981
MaeIII GTNAC 3 cut(s) 871, 1031, 1145
MalI GATC 5 cut(s) 381, 396, 475, 1020, 1338
MboI GATC 5 cut(s) 379, 394, 473, 1018, 1336
MboII GAAGA 6 cut(s) 113, 116, 811, 1004, 1207, 1361
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 394, 1336
MhlI GDGCHC 3 cut(s) 538, 566, 588
MluCI AATT 6 cut(s) 401, 431, 741, 768, 879, 1279
MlyI GAGTC 3 cut(s) 629, 837, 1028
MnlI CCTC 7 cut(s) 49, 106, 349, 911, 932, 938, 1184
Mph1103I ATGCAT 1 cut(s) 615
MseI TTAA 4 cut(s) 171, 792, 930, 1326
MslI CAYNNNNRTG 1 cut(s) 412
MspA1I CMGCKG 2 cut(s) 864, 1067
MspR9I CCNGG 4 cut(s) 275, 672, 819, 1190
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 2 cut(s) 69, 1129
MvaI CCWGG 4 cut(s) 275, 672, 819, 1190
MwoI GCNNNNNNNGC 1 cut(s) 542
NdeII GATC 5 cut(s) 379, 394, 473, 1018, 1336
NlaIV GGNNCC 6 cut(s) 6, 31, 279, 396, 669, 968
NmeAIII GCCGAG 1 cut(s) 644
NmuCI GTSAC 3 cut(s) 871, 1031, 1145
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 1 cut(s) 252
PaeI GCATGC 1 cut(s) 252
PagI TCATGA 1 cut(s) 1285
PcsI WCGNNNNNNNCGW 1 cut(s) 967
PctI GAATGC 2 cut(s) 69, 1129
PfeI GAWTC 5 cut(s) 130, 696, 724, 804, 1107
Pfl23II CGTACG 1 cut(s) 640
PflMI CCANNNNNTGG 1 cut(s) 824
PfoI TCCNGGA 1 cut(s) 670
PkrI GCNGC 3 cut(s) 293, 866, 1163
PleI GAGTC 3 cut(s) 629, 836, 1028
PpsI GAGTC 3 cut(s) 629, 836, 1028
Psp124BI GAGCTC 1 cut(s) 566
Psp6I CCWGG 4 cut(s) 273, 670, 817, 1188
PspGI CCWGG 4 cut(s) 273, 670, 817, 1188
PspLI CGTACG 1 cut(s) 640
PspN4I GGNNCC 6 cut(s) 6, 31, 279, 396, 669, 968
PspPI GGNCC 4 cut(s) 29, 323, 815, 967
PstI CTGCAG 1 cut(s) 1145
PsuI RGATCY 2 cut(s) 394, 1336
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 3 cut(s) 279, 444, 642
RsaNI GTAC 3 cut(s) 278, 443, 641
RseI CAYNNNNRTG 1 cut(s) 412
SacI GAGCTC 1 cut(s) 566
SaqAI TTAA 4 cut(s) 171, 792, 930, 1326
SatI GCNGC 3 cut(s) 292, 865, 1162
Sau3AI GATC 5 cut(s) 379, 394, 473, 1018, 1336
Sau96I GGNCC 4 cut(s) 29, 323, 815, 967
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 3 cut(s) 629, 837, 1028
ScrFI CCNGG 4 cut(s) 275, 672, 819, 1190
SduI GDGCHC 3 cut(s) 538, 566, 588
SfaNI GCATC 5 cut(s) 719, 771, 843, 1267, 1321
SfcI CTRYAG 3 cut(s) 540, 1141, 1317
SinI GGWCC 2 cut(s) 815, 967
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
SphI GCATGC 1 cut(s) 252
Sse9I AATT 6 cut(s) 401, 431, 741, 768, 879, 1279
SsiI CCGC 4 cut(s) 292, 813, 864, 925
SspI AATATT 1 cut(s) 790
SspMI CTAG 3 cut(s) 299, 845, 1169
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 4 cut(s) 273, 670, 817, 1188
StyI CCWWGG 2 cut(s) 283, 451
TaaI ACNGT 4 cut(s) 680, 1031, 1153, 1210
TaiI ACGT 3 cut(s) 123, 154, 984
TasI AATT 6 cut(s) 401, 431, 741, 768, 879, 1279
TatI WGTACW 1 cut(s) 442
TauI GCSGC 2 cut(s) 294, 867
TfiI GAWTC 5 cut(s) 130, 696, 724, 804, 1107
Tru1I TTAA 4 cut(s) 171, 792, 930, 1326
Tru9I TTAA 4 cut(s) 171, 792, 930, 1326
TscAI CASTG 5 cut(s) 40, 694, 712, 1150, 1213
TseFI GTSAC 3 cut(s) 871, 1031, 1145
TseI GCWGC 1 cut(s) 1161
Tsp45I GTSAC 3 cut(s) 871, 1031, 1145
TspDTI ATGAA 4 cut(s) 13, 486, 812, 1145
TspGWI ACGGA 3 cut(s) 123, 628, 959
TspRI CASTG 5 cut(s) 40, 694, 712, 1150, 1213
Van91I CCANNNNNTGG 1 cut(s) 824
VpaK11BI GGWCC 2 cut(s) 815, 967
XapI RAATTY 2 cut(s) 401, 1279
XceI RCATGY 1 cut(s) 252
XspI CTAG 3 cut(s) 299, 845, 1169
ZraI GACGTC 1 cut(s) 121
ZrmI AGTACT 1 cut(s) 444
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.