FvH4_4g32840
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004713
Physical Location & Seq
Reverse (-)
31312524 .. 31316857
4334 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32840.t2

Sequence Viewer

Length: 1392 bp
ATGAATAGATTCTTTGTGATTTCTTTTCTTGGTATGCTGATAGCAGTTCATGGAGTTGCAGGAAACATGATCTATGATGACATTGGCACGCTTGATGAGCTTGAAAACTTTGACATAGAAGAAGACGATGAGGTGGATGTCTTTGAAATCCCGTCATGGACAAGCGAACGTGGTAGCAAGGTTCTTGTTAATGTGGATAGCTTTGGTGCTATTGGAGATGGAGTTTCTGATGATACTCAGGCTTTTGTAAAAGCTTGGGATATTGCATGCAATACAACAAAAGCTGTTTTCCTGGTACCCCAAGGGCGCCGCTATCTAGTAAATGCAACAAGATTTGAAGGGCCATGCGCAGATAGCTTAGTCATCCAGATTGAGGGAACAATAGTAGCACCAGATGATCCTGATAACTGGGATCCCAAATTTCCACGAACATGGCTTGATTTCTATAAACTGAAGGGAGTACTTTTCCAAGGTCATGGAGTTATTGATGGATCAGGCAGTAAATGGTGGGCTTCATCCTGCAAAAAGAATAAGACAAATCCTTGCAGGGGTGCTCCAACAGCATTTACTATTGATAGAAGCTCAGCTATAAAGGTAAAAGGGCTCACCATCCAGAACAGCCAACAAATGAATTTCGCCATCTCTCAAAGTGATTCTGTACGAGTTTATGCAGTCCAAGTCTCATCTCCAGGAGACAGTCCTAACACTGATGGAATCCATATAACTGCATCCACTAATGTGATTCTTCAAGACTGCAAAATTGGAACAGGAGATGACTGCATCTCAATTGTCAATGCTACATCAAATATTAAGATGAAGAGAATCCTTTGTGGACCAGGACATGGAGTCAGCATTGGAAGTCTAGGGAAGGACAATTCCAGCGCCGTAGTCACAAAAGTGGTCTTGGATACAGCATTCCTCAGGGGGACTACCAATGGCCTCCGCATCAAGACTTGGCAGGGAGGTTCTGGTTATGTTCGTGGAGTCCGTTTCCAAAATGTAAGGATGGAAGATGTAGCGAACCCCATAATCATAGATCAGTTCTACTGTGACTCCCCCAAAGCTTGTCAAAATCAGACATCAGCTGTGCAAATCAGCCAAATCATGTATTGGAACGTCTCAGGAACTACAACGAGTAAGAATGCGATGAAGTTTGCCTGCAGTGACACAGTTCCATGCAGCAACCTAGTCCTCAGTAACGTCAACTTGGAGAAGAAAGATGGCACAGTGGAGACATATTGCAACTCTGCCGAAGGCTTCGGGACTGGAGTTGTGCATCCATCAGCAGATTGTCTGAACTCTCATGACAAAGATATGATAGTCATCAAACAGCCTGCAGAAGTTGAACATGCAGATCCCAGCACAGAAAAAATTGTGCATACTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

464

Amino Acids

50.25

Weight (kDa)

5.12

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 394 1.9e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 349
Acc65I GGTACC 1 cut(s) 295
AccB1I GGYRCC 2 cut(s) 295, 306
AccB7I CCANNNNNTGG 1 cut(s) 842
AciI CCGC 2 cut(s) 310, 943
AclWI GGATC 5 cut(s) 392, 407, 420, 499, 1349
AcsI RAATTY 2 cut(s) 419, 631
AcuI CTGAAG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 307
AfaI GTAC 3 cut(s) 297, 462, 660
AfiI CCNNNNNNNGG 3 cut(s) 373, 548, 842
AgsI TTSAA 5 cut(s) 104, 146, 338, 749, 1346
AhdI GACNNNNNGTC 1 cut(s) 845
AjnI CCWGG 3 cut(s) 291, 688, 835
AleI CACNNNNGTG 2 cut(s) 737, 896
AluBI AGCT 9 cut(s) 100, 201, 254, 284, 357, 582, 587, 1064, 1085
AluI AGCT 9 cut(s) 100, 201, 254, 284, 357, 582, 587, 1064, 1085
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 4 cut(s) 685, 687, 1123, 1226
AlwI GGATC 5 cut(s) 392, 407, 420, 499, 1349
AoxI GGCC 2 cut(s) 341, 937
ApeKI GCWGC 1 cut(s) 1179
ApoI RAATTY 2 cut(s) 419, 631
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 295
AspLEI GCGC 3 cut(s) 309, 350, 884
AspS9I GGNCC 2 cut(s) 341, 833
AsuHPI GGTGA 1 cut(s) 598
AvaII GGWCC 1 cut(s) 833
AxyI CCTNAGG 1 cut(s) 920
BamHI GGATCC 1 cut(s) 412
BanI GGYRCC 2 cut(s) 295, 306
BanII GRGCYC 1 cut(s) 606
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 1 cut(s) 1191
BccI CCATC 8 cut(s) 212, 482, 617, 647, 704, 1000, 1214, 1288
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 3 cut(s) 293, 690, 837
BciVI GTATCC 1 cut(s) 901
BcoDI GTCTC 4 cut(s) 685, 687, 1123, 1226
BfaI CTAG 3 cut(s) 317, 863, 1187
BfmI CTRYAG 2 cut(s) 1159, 1335
BfoI RGCGCY 2 cut(s) 310, 885
BfuI GTATCC 1 cut(s) 901
BisI GCNGC 2 cut(s) 310, 1180
BlpI GCTNAGC 1 cut(s) 583
BlsI GCNGC 2 cut(s) 311, 1181
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 3 cut(s) 293, 690, 837
Bme18I GGWCC 1 cut(s) 833
BmeRI GACNNNNNGTC 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 341, 833
BmiI GGNNCC 3 cut(s) 297, 308, 414
BmrFI CCNGG 3 cut(s) 293, 690, 837
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 4 cut(s) 737, 789, 954, 1285
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 2 cut(s) 672, 1287
Bpu1102I GCTNAGC 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 1322
BsaHI GRCGYC 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 301, 469
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 1037, 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 548, 842
Bse1I ACTGG 2 cut(s) 413, 1270
Bse21I CCTNAGG 1 cut(s) 920
Bse8I GATNNNNATC 1 cut(s) 1322
BseBI CCWGG 3 cut(s) 293, 690, 837
BseDI CCNNGG 2 cut(s) 301, 469
BseGI GGATG 7 cut(s) 142, 363, 515, 609, 728, 1011, 1276
BseJI GATNNNNATC 1 cut(s) 1322
BseLI CCNNNNNNNGG 3 cut(s) 373, 548, 842
BseMII CTCAG 5 cut(s) 251, 597, 934, 1134, 1207
BseNI ACTGG 2 cut(s) 413, 1270
BseXI GCAGC 1 cut(s) 1191
BseYI CCCAGC 1 cut(s) 1358
BshFI GGCC 2 cut(s) 343, 939
BshNI GGYRCC 2 cut(s) 295, 306
BsiHKAI GWGCWC 1 cut(s) 556
BslFI GGGAC 2 cut(s) 940, 1276
BslI CCNNNNNNNGG 3 cut(s) 373, 548, 842
BsmAI GTCTC 4 cut(s) 685, 687, 1123, 1226
BsmBI CGTCTC 1 cut(s) 1123
BsmFI GGGAC 2 cut(s) 940, 1276
BsmI GAATGC 2 cut(s) 914, 1147
BsnI GGCC 2 cut(s) 343, 939
Bsp1286I GDGCHC 2 cut(s) 556, 606
Bsp143I GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
Bsp1720I GCTNAGC 1 cut(s) 583
BspACI CCGC 2 cut(s) 310, 943
BspANI GGCC 2 cut(s) 343, 939
BspCNI CTCAG 5 cut(s) 250, 596, 933, 1133, 1206
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 297, 308, 414
BspMAI CTGCAG 2 cut(s) 1163, 1339
BspPI GGATC 5 cut(s) 392, 407, 420, 499, 1349
BspT107I GGYRCC 2 cut(s) 295, 306
BsrI ACTGG 2 cut(s) 413, 1270
BssECI CCNNGG 2 cut(s) 301, 469
BssMI GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
BssNI GRCGYC 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 301, 469
Bst2UI CCWGG 3 cut(s) 293, 690, 837
Bst4CI ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1389
Bst6I CTCTTC 1 cut(s) 812
BstACI GRCGYC 1 cut(s) 307
BstC8I GCNNGC 4 cut(s) 89, 268, 1159, 1335
BstDEI CTNAG 6 cut(s) 237, 358, 583, 920, 1120, 1193
BstF5I GGATG 7 cut(s) 142, 363, 515, 609, 728, 1011, 1276
BstH2I RGCGCY 2 cut(s) 310, 885
BstHHI GCGC 3 cut(s) 309, 350, 884
BstKTI GATC 6 cut(s) 72, 400, 415, 494, 1039, 1357
BstMAI GTCTC 4 cut(s) 685, 687, 1123, 1226
BstMBI GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
BstMWI GCNNNNNNNGC 3 cut(s) 97, 354, 560
BstNI CCWGG 3 cut(s) 293, 690, 837
BstNSI RCATGY 2 cut(s) 270, 1352
BstSCI CCNGG 3 cut(s) 291, 688, 835
BstSFI CTRYAG 2 cut(s) 1159, 1335
BstV1I GCAGC 1 cut(s) 1191
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 412, 1354
BstXI CCANNNNNNTGG 2 cut(s) 432, 476
BstYI RGATCY 2 cut(s) 412, 1354
Bsu36I CCTNAGG 1 cut(s) 920
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 2 cut(s) 343, 939
BtgZI GCGATG 1 cut(s) 1160
BtsCI GGATG 7 cut(s) 142, 363, 515, 609, 728, 1011, 1276
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 3 cut(s) 705, 1168, 1233
Cac8I GCNNGC 4 cut(s) 89, 268, 1159, 1335
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 3 cut(s) 309, 350, 884
Cfr13I GGNCC 2 cut(s) 341, 833
Csp6I GTAC 3 cut(s) 296, 461, 659
CviQI GTAC 3 cut(s) 296, 461, 659
DdeI CTNAG 6 cut(s) 237, 358, 583, 920, 1120, 1193
DinI GGCGCC 1 cut(s) 308
DpnI GATC 6 cut(s) 71, 399, 414, 493, 1038, 1356
DpnII GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
DriI GACNNNNNGTC 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 812
Eam1105I GACNNNNNGTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 812
Eco130I CCWWGG 2 cut(s) 301, 469
Eco24I GRGCYC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 833
Eco57I CTGAAG 1 cut(s) 473
Eco81I CCTNAGG 1 cut(s) 920
EcoRII CCWGG 3 cut(s) 291, 688, 835
EcoT14I CCWWGG 2 cut(s) 301, 469
EcoT38I GRGCYC 1 cut(s) 606
EgeI GGCGCC 1 cut(s) 308
EheI GGCGCC 1 cut(s) 308
ErhI CCWWGG 2 cut(s) 301, 469
Esp3I CGTCTC 1 cut(s) 1123
FaqI GGGAC 2 cut(s) 940, 1276
Fnu4HI GCNGC 2 cut(s) 310, 1180
FokI GGATG 7 cut(s) 149, 350, 502, 596, 715, 1018, 1263
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 2 cut(s) 310, 1180
FspBI CTAG 3 cut(s) 317, 863, 1187
FspI TGCGCA 1 cut(s) 349
GlaI GCGC 3 cut(s) 308, 349, 883
GluI GCNGC 2 cut(s) 310, 1180
GsaI CCCAGC 1 cut(s) 1362
GsuI CTGGAG 2 cut(s) 672, 1287
HaeII RGCGCY 2 cut(s) 310, 885
HaeIII GGCC 2 cut(s) 343, 939
HhaI GCGC 3 cut(s) 309, 350, 884
Hin1I GRCGYC 1 cut(s) 307
Hin6I GCGC 3 cut(s) 307, 348, 882
HinP1I GCGC 3 cut(s) 307, 348, 882
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HindIII AAGCTT 2 cut(s) 252, 1062
HinfI GANTC 8 cut(s) 9, 653, 714, 742, 822, 846, 984, 1052
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 2 cut(s) 833, 1204
Hpy188I TCNGA 3 cut(s) 229, 1077, 1296
Hpy188III TCNNGA 8 cut(s) 367, 401, 613, 749, 949, 1122, 1261, 1304
Hpy8I GTNNAC 2 cut(s) 833, 1204
HpyAV CCTTC 4 cut(s) 332, 448, 862, 1247
HpyCH4III ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1389
HpyCH4IV ACGT 3 cut(s) 169, 1116, 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 354, 560
HpyF3I CTNAG 6 cut(s) 237, 358, 583, 920, 1120, 1193
HpySE526I ACGT 3 cut(s) 169, 1116, 1200
Hsp92I GRCGYC 1 cut(s) 307
HspAI GCGC 3 cut(s) 307, 348, 882
KasI GGCGCC 1 cut(s) 306
KpnI GGTACC 1 cut(s) 299
Kzo9I GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
LmnI GCTCC 1 cut(s) 559
Lsp1109I GCAGC 1 cut(s) 1191
LweI GCATC 4 cut(s) 737, 789, 954, 1285
MaeI CTAG 3 cut(s) 317, 863, 1187
MaeII ACGT 3 cut(s) 169, 1116, 1200
MaeIII GTNAC 4 cut(s) 889, 1049, 1163, 1196
MalI GATC 6 cut(s) 71, 399, 414, 493, 1038, 1356
MboI GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
MboII GAAGA 6 cut(s) 131, 134, 737, 829, 1022, 1225
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 2 cut(s) 412, 1354
MhlI GDGCHC 2 cut(s) 556, 606
MluCI AATT 6 cut(s) 419, 631, 759, 786, 874, 1371
Mly113I GGCGCC 1 cut(s) 307
MlyI GAGTC 3 cut(s) 855, 993, 1046
MmeI TCCRAC 1 cut(s) 581
MnlI CCTC 6 cut(s) 124, 367, 929, 950, 956, 1202
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 189, 810
MslI CAYNNNNRTG 3 cut(s) 430, 737, 896
MspA1I CMGCKG 1 cut(s) 1085
MspR9I CCNGG 3 cut(s) 293, 690, 837
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 2 cut(s) 914, 1147
MvaI CCWGG 3 cut(s) 293, 690, 837
MwoI GCNNNNNNNGC 3 cut(s) 97, 354, 560
NarI GGCGCC 1 cut(s) 307
NdeII GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
NlaIV GGNNCC 3 cut(s) 297, 308, 414
NmuCI GTSAC 3 cut(s) 889, 1049, 1163
NsbI TGCGCA 1 cut(s) 349
NspI RCATGY 2 cut(s) 270, 1352
OliI CACNNNNGTG 2 cut(s) 737, 896
PaeI GCATGC 1 cut(s) 270
PagI TCATGA 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PctI GAATGC 2 cut(s) 914, 1147
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 5 cut(s) 9, 653, 714, 742, 822
PflMI CCANNNNNTGG 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 2 cut(s) 311, 1181
PleI GAGTC 3 cut(s) 854, 992, 1046
PluTI GGCGCC 1 cut(s) 310
PpsI GAGTC 3 cut(s) 854, 992, 1046
Psp6I CCWGG 3 cut(s) 291, 688, 835
PspFI CCCAGC 1 cut(s) 1358
PspGI CCWGG 3 cut(s) 291, 688, 835
PspN4I GGNNCC 3 cut(s) 297, 308, 414
PspPI GGNCC 2 cut(s) 341, 833
PstI CTGCAG 2 cut(s) 1163, 1339
PsuI RGATCY 2 cut(s) 412, 1354
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 3 cut(s) 297, 462, 660
RsaNI GTAC 3 cut(s) 296, 461, 659
RseI CAYNNNNRTG 3 cut(s) 430, 737, 896
SaqAI TTAA 2 cut(s) 189, 810
SatI GCNGC 2 cut(s) 310, 1180
Sau3AI GATC 6 cut(s) 69, 397, 412, 491, 1036, 1354
Sau96I GGNCC 2 cut(s) 341, 833
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 3 cut(s) 855, 993, 1046
ScrFI CCNGG 3 cut(s) 293, 690, 837
SduI GDGCHC 2 cut(s) 556, 606
SfaNI GCATC 4 cut(s) 737, 789, 954, 1285
SfcI CTRYAG 2 cut(s) 1159, 1335
SfoI GGCGCC 1 cut(s) 308
SinI GGWCC 1 cut(s) 833
SmiMI CAYNNNNRTG 3 cut(s) 430, 737, 896
SphI GCATGC 1 cut(s) 270
Sse9I AATT 6 cut(s) 419, 631, 759, 786, 874, 1371
SsiI CCGC 2 cut(s) 310, 943
SspDI GGCGCC 1 cut(s) 306
SspI AATATT 1 cut(s) 808
SspMI CTAG 3 cut(s) 317, 863, 1187
StyD4I CCNGG 3 cut(s) 291, 688, 835
StyI CCWWGG 2 cut(s) 301, 469
TaaI ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1389
TaiI ACGT 3 cut(s) 172, 1119, 1203
TasI AATT 6 cut(s) 419, 631, 759, 786, 874, 1371
TatI WGTACW 1 cut(s) 460
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 5 cut(s) 9, 653, 714, 742, 822
Tru1I TTAA 2 cut(s) 189, 810
Tru9I TTAA 2 cut(s) 189, 810
TscAI CASTG 3 cut(s) 712, 1168, 1233
TseFI GTSAC 3 cut(s) 889, 1049, 1163
TseI GCWGC 1 cut(s) 1179
Tsp45I GTSAC 3 cut(s) 889, 1049, 1163
TspDTI ATGAA 6 cut(s) 17, 38, 504, 644, 830, 1163
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 3 cut(s) 712, 1168, 1233
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 1 cut(s) 833
XapI RAATTY 2 cut(s) 419, 631
XceI RCATGY 2 cut(s) 270, 1352
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 3 cut(s) 317, 863, 1187
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.