AT1G27385

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005463
Physical Location & Seq
Reverse (-)
9510475 .. 9512971
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27385.1

Sequence Viewer

Length: 609 bp
ATGGCTTGCAAAACGATTTTGCGTTCCGTTTTCGTATCGGAATCACGACGGACTTCCGGCGCACGTAGATGCTTCTTTCTCCCTCCTTCTCCAGCATCAGTTCCAGTCCATGGTTTGTTCCCGGCGCCAAAGAGTCTATGTTTCAATGGTTTTGCATCTGTTCCCGAGCGGGCTACTCGGCTTAATTGCAGCCATAACGATCAATCCGATCAAGGTCCGCCTCAAGAAGCTGTTCTCAAGGCAATCTCAGAAGTGTCAAAGACTGATGGGAGGGTTGGGAAAACTACTAACATGATTATTGGAGGCACTGTGGCTGATGATTCTGCTAAAGATTGGCTCGAACTTGACCAAAAGGTCAATACATACCCAACAGAAAGAGGGTTTACTGCAATTGGGACTGGTGGCAATGACTTTGTGCATGCTATGGTTGTTGCTGTTGAATCTGTTATTGACCGACAAATTCCAGAGGACTGTGTAAAGCAGACTTTATCAAGCAAAGGAAAATATGTGTCGGTGAATATTGGCCCTATCCAAGTCGTCTCTAGTGAGCAGGTTCAAGCTGTGTATAATGCAATGAGGAGAGACGAAAGAATGAAATACTTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

202

Amino Acids

22.06

Weight (kDa)

8.61

Isoelectric Point (pI)

52.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF493 PF04359 119 - 202 4.7e-20 Protein of unknown function (DUF493)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 541
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 2 cut(s) 169, 218
AcsI RAATTY 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 3 cut(s) 145, 440, 557
AluBI AGCT 2 cut(s) 230, 560
AluI AGCT 2 cut(s) 230, 560
Alw26I GTCTC 2 cut(s) 544, 576
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 1 cut(s) 459
Asp700I GAANNNNTTC 2 cut(s) 231, 599
AspLEI GCGC 2 cut(s) 62, 127
AspS9I GGNCC 2 cut(s) 215, 524
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 260
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 2 cut(s) 544, 576
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 541
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 215, 524
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 3 cut(s) 59, 104, 164
BpmI CTGGAG 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 207, 221
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 104, 403
Bse3DI GCAATG 2 cut(s) 412, 579
BseDI CCNNGG 1 cut(s) 109
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 2 cut(s) 412, 579
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 104, 403
BseRI GAGGAG 1 cut(s) 592
BseXI GCAGC 1 cut(s) 201
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 57, 122
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 2 cut(s) 544, 576
BsmBI CGTCTC 2 cut(s) 544, 576
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 164
Bsp143I GATC 2 cut(s) 199, 208
Bsp19I CCATGG 1 cut(s) 109
BspACI CCGC 2 cut(s) 169, 218
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 1 cut(s) 260
BspLI GGNNCC 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 541
BspT107I GGYRCC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 2 cut(s) 412, 579
BsrI ACTGG 2 cut(s) 104, 403
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 2 cut(s) 199, 208
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 310, 473
BstACI GRCGYC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 7, 171, 420
BstDEI CTNAG 1 cut(s) 247
BstDSI CCRYGG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 62, 127
BstKTI GATC 2 cut(s) 202, 211
BstMAI GTCTC 2 cut(s) 544, 576
BstMBI GATC 2 cut(s) 199, 208
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 201
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 3 cut(s) 7, 171, 420
CfoI GCGC 2 cut(s) 62, 127
Cfr13I GGNCC 2 cut(s) 215, 524
CviAII CATG 3 cut(s) 110, 292, 419
CviJI RGCY 9 cut(s) 5, 173, 181, 192, 230, 314, 337, 525, 560
CviKI_1 RGCY 9 cut(s) 5, 173, 181, 192, 230, 314, 337, 525, 560
DdeI CTNAG 1 cut(s) 247
DinI GGCGCC 1 cut(s) 126
DpnI GATC 2 cut(s) 201, 210
DpnII GATC 2 cut(s) 199, 208
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 215
Eco88I CYCGRG 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 109
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 109
Esp3I CGTCTC 2 cut(s) 544, 576
FaeI CATG 3 cut(s) 113, 295, 422
FaiI YATR 9 cut(s) 111, 139, 195, 293, 364, 420, 425, 507, 567
FaqI GGGAC 1 cut(s) 409
FatI CATG 3 cut(s) 109, 291, 418
FauI CCCGC 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 190
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 1 cut(s) 543
GlaI GCGC 2 cut(s) 61, 126
GluI GCNGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 1 cut(s) 525
HapII CCGG 2 cut(s) 57, 122
HhaI GCGC 2 cut(s) 62, 127
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 113, 295, 422
Hin6I GCGC 2 cut(s) 60, 125
HinP1I GCGC 2 cut(s) 60, 125
HinfI GANTC 4 cut(s) 41, 133, 320, 440
HpaII CCGG 2 cut(s) 57, 122
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 3 cut(s) 40, 208, 250
Hpy188III TCNNGA 4 cut(s) 45, 164, 224, 464
Hpy8I GTNNAC 1 cut(s) 384
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 2 cut(s) 310, 473
HpyCH4IV ACGT 1 cut(s) 64
HpyCH4V TGCA 6 cut(s) 9, 155, 189, 389, 418, 572
HpyF3I CTNAG 1 cut(s) 247
HpySE526I ACGT 1 cut(s) 64
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 113, 295, 422
HspAI GCGC 2 cut(s) 60, 125
KasI GGCGCC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 199, 208
LpnPI CCDG 7 cut(s) 70, 105, 117, 135, 384, 477, 536
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 3 cut(s) 59, 104, 164
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 64
MalI GATC 2 cut(s) 201, 210
MbiI CCGCTC 1 cut(s) 169
MboI GATC 2 cut(s) 199, 208
MfeI CAATTG 1 cut(s) 390
MluCI AATT 3 cut(s) 184, 390, 459
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 7 cut(s) 93, 231, 264, 296, 371, 460, 570
MroXI GAANNNNTTC 2 cut(s) 231, 599
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 67
MspI CCGG 2 cut(s) 57, 122
MspR9I CCNGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 390
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 109
NdeII GATC 2 cut(s) 199, 208
NlaIII CATG 3 cut(s) 113, 295, 422
NlaIV GGNNCC 1 cut(s) 126
NmeAIII GCCGAG 1 cut(s) 157
NspI RCATGY 1 cut(s) 422
PaeI GCATGC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 231, 599
PfeI GAWTC 3 cut(s) 41, 320, 440
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 1 cut(s) 191
PleI GAGTC 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 65
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 2 cut(s) 215, 524
RseI CAYNNNNRTG 1 cut(s) 67
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 2 cut(s) 199, 208
Sau96I GGNCC 2 cut(s) 215, 524
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 122
SetI ASST 6 cut(s) 67, 217, 232, 357, 555, 562
SfaNI GCATC 3 cut(s) 59, 104, 164
SfoI GGCGCC 1 cut(s) 126
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 2 cut(s) 222, 236
SmoI CTYRAG 2 cut(s) 222, 236
SphI GCATGC 1 cut(s) 422
Sse9I AATT 3 cut(s) 184, 390, 459
SsiI CCGC 2 cut(s) 169, 218
SspDI GGCGCC 1 cut(s) 124
SspI AATATT 1 cut(s) 520
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 2 cut(s) 310, 473
TaiI ACGT 1 cut(s) 67
TaqI TCGA 1 cut(s) 339
TaqII GACCGA 1 cut(s) 468
TasI AATT 3 cut(s) 184, 390, 459
TfiI GAWTC 3 cut(s) 41, 320, 440
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 189
TspDTI ATGAA 1 cut(s) 608
TspGWI ACGGA 2 cut(s) 16, 64
TspRI CASTG 1 cut(s) 313
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 459
XceI RCATGY 1 cut(s) 422
XmnI GAANNNNTTC 2 cut(s) 231, 599
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.