RLG00000006994

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005463
Physical Location & Seq
Forward (+)
12534261 .. 12536713
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006994

Sequence Viewer

Length: 606 bp
ATGGCTTGCAGAACTGCCTTGCGCTCCGCTCTTCTAATAGAAGCGTGGCGACCCGTTCACCTCTCTCGGAGCTCCGTTTCCGCTTCGCTCAGAACCCGAAACGGATTCTCCTCCCCGCAGCTGACCCGCCAATTCAAGACCCGAGACGTCCAGTTGGCCAAAGGGACCCAACTCAGTTGCTCCCACAACGAAAACCAACCCGCTCTCTCCGAGGACGAACAAGGCCCGCCTCAGGAGGCCGTTCTCAAAGCTATTTCAGAGGTGTCTAGAACTGAAGGGAGGGTTGGGCAGACCACGAATGTGGTTATTGGAGGCACGGTGGCCGATGATTCCACCACTGAATGGCTCGACTTGGATCAGAAGGTAAATTCATACCCAACTGTTAGAGGGTTCACGGCGATTGGAACCGGAGGGGATGATTTTGTGCAGGCTATGGTTGTTGCTGTTGAATCCGTACTTCAACAACCCGTTCCTGAGGGTCGTGTCAAGCAGAAGCTATCATCAGGAGGCAAATATGTGTCTGTAAACATTGGTCCTGTTCAAGTCATTTCTAGTGAACAGGTCTTCCCCTCCCTCCCTCCCTCCCTCCCTGCCTCACAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

202

Amino Acids

21.58

Weight (kDa)

6.74

Isoelectric Point (pI)

61.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF493 PF04359 122 - 188 1.1e-13 Protein of unknown function (DUF493)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 342
AccBSI CCGCTC 2 cut(s) 29, 203
AciI CCGC 6 cut(s) 27, 81, 116, 127, 201, 227
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 2 cut(s) 156, 321
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 294
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 2 cut(s) 232, 342
AgsI TTSAA 4 cut(s) 136, 449, 461, 542
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AleI CACNNNNGTG 1 cut(s) 299
AloI GAACNNNNNNTCC 2 cut(s) 549, 581
AluBI AGCT 4 cut(s) 72, 121, 251, 496
AluI AGCT 4 cut(s) 72, 121, 251, 496
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 4 cut(s) 156, 223, 237, 321
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 3 cut(s) 165, 224, 533
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 2 cut(s) 165, 533
AxyI CCTNAGG 2 cut(s) 231, 474
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 130
BceAI ACGGC 2 cut(s) 224, 411
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 2 cut(s) 267, 552
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 165, 533
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 3 cut(s) 165, 224, 533
BmiI GGNNCC 3 cut(s) 166, 167, 406
BpiI GAAGAC 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 303, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 342
Bse1I ACTGG 1 cut(s) 151
Bse21I CCTNAGG 2 cut(s) 231, 474
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 232, 342
BseMII CTCAG 4 cut(s) 103, 187, 245, 465
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 446
BshFI GGCC 4 cut(s) 158, 225, 239, 323
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 232, 342
BsmAI GTCTC 1 cut(s) 138
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 4 cut(s) 158, 225, 239, 323
BsoBI CYCGRG 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 1 cut(s) 355
BspACI CCGC 6 cut(s) 27, 81, 116, 127, 201, 227
BspANI GGCC 4 cut(s) 158, 225, 239, 323
BspCNI CTCAG 4 cut(s) 102, 186, 244, 466
BspLI GGNNCC 3 cut(s) 166, 167, 406
BspPI GGATC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 36
BsrBI CCGCTC 2 cut(s) 29, 203
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 355
BssNI GRCGYC 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 319, 382
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 7, 227, 429
BstDEI CTNAG 4 cut(s) 89, 173, 231, 474
BstF5I GGATG 1 cut(s) 421
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 556
BstXI CCANNNNNNTGG 1 cut(s) 301
Bsu36I CCTNAGG 2 cut(s) 231, 474
BsuRI GGCC 4 cut(s) 158, 225, 239, 323
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 7, 227, 429
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 3 cut(s) 165, 224, 533
Csp6I GTAC 1 cut(s) 455
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 4 cut(s) 89, 173, 231, 474
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
EaeI YGGCCR 2 cut(s) 156, 321
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 74
Eco47I GGWCC 2 cut(s) 165, 533
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 2 cut(s) 231, 474
Eco88I CYCGRG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 138
FaiI YATR 3 cut(s) 373, 434, 516
FaqI GGGAC 1 cut(s) 178
FauI CCCGC 4 cut(s) 123, 134, 208, 234
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 2 cut(s) 267, 552
GlaI GCGC 1 cut(s) 23
GluI GCNGC 1 cut(s) 119
HaeIII GGCC 4 cut(s) 158, 225, 239, 323
HapII CCGG 1 cut(s) 408
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HinfI GANTC 3 cut(s) 105, 329, 449
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 4 cut(s) 58, 393, 526, 557
Hpy188I TCNGA 5 cut(s) 69, 92, 211, 259, 360
Hpy188III TCNNGA 5 cut(s) 136, 233, 267, 473, 504
Hpy8I GTNNAC 4 cut(s) 58, 393, 526, 557
HpyAV CCTTC 2 cut(s) 269, 355
HpyCH4III ACNGT 2 cut(s) 319, 382
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 9, 427
HpyF3I CTNAG 4 cut(s) 89, 173, 231, 474
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 1 cut(s) 22
KflI GGGWCCC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 355
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 29, 69, 77, 185
LpnPI CCDG 8 cut(s) 164, 218, 413, 421, 486, 489, 545, 549
Lsp1109I GCAGC 1 cut(s) 130
MaeI CTAG 2 cut(s) 267, 552
MaeII ACGT 1 cut(s) 147
MalI GATC 1 cut(s) 357
MbiI CCGCTC 2 cut(s) 29, 203
MboI GATC 1 cut(s) 355
MboII GAAGA 2 cut(s) 23, 556
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 3 cut(s) 131, 367, 599
MluNI TGGCCA 1 cut(s) 158
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MseI TTAA 1 cut(s) 604
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 1 cut(s) 408
NdeII GATC 1 cut(s) 355
NlaIV GGNNCC 3 cut(s) 166, 167, 406
OliI CACNNNNGTG 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 105, 329, 449
PflMI CCANNNNNTGG 1 cut(s) 342
PkrI GCNGC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 166, 167, 406
PspPI GGNCC 3 cut(s) 165, 224, 533
PspPPI RGGWCCY 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 604
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 3 cut(s) 165, 224, 533
SduI GDGCHC 1 cut(s) 74
SetI ASST 9 cut(s) 63, 74, 123, 150, 253, 264, 366, 498, 564
SinI GGWCC 2 cut(s) 165, 533
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 3 cut(s) 131, 367, 599
SsiI CCGC 6 cut(s) 27, 81, 116, 127, 201, 227
SspMI CTAG 2 cut(s) 267, 552
SstI GAGCTC 1 cut(s) 74
TaaI ACNGT 2 cut(s) 319, 382
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 348
TasI AATT 3 cut(s) 131, 367, 599
TfiI GAWTC 3 cut(s) 105, 329, 449
Tru1I TTAA 1 cut(s) 604
Tru9I TTAA 1 cut(s) 604
TscAI CASTG 1 cut(s) 343
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 3 cut(s) 64, 117, 442
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 342
VpaK11BI GGWCC 2 cut(s) 165, 533
XapI RAATTY 1 cut(s) 367
XbaI TCTAGA 1 cut(s) 266
XspI CTAG 2 cut(s) 267, 552
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.