Rh4AG298000

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005463
Physical Location & Seq
Reverse (-)
61122320 .. 61125105
2786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG298000.1

Sequence Viewer

Length: 618 bp
ATGGCTTGCAGAACTGCCTTGCGCTCCGCTCTTCTAATAGAAGCGTGGCGACCCGTTCACCTCGCTCGGAGCTCCGTTTCCTCTTCGCTCCGAACCCGAAACGGATTCTCCTCCCCGCAGCTGACCCGCCAATTCAAGACCCGAGACGTCCACTTGGCCAAACGGACCCAACTCAGTTGCTCCCACAACGAAAACCAACCCGCTCTCTCCGAGGACGAACAAGGCCCGCCTCAGGAGGCCGTTCTCAAAGCTATTTCAGAGGTGTCTAGAACTGAAGGGAGGGTCGGGCAGACCACGAATGTGGTTATTGGAGGCACGGTGGCCGATGATTCCACCACTGAATGGCTCGACTTGGATCAGAAGGTAAATTCATACCCAACTGTTAGAGGGTTCACGGCGATTGGAACCGGAGGGGATGATTTTGTGCAGGCTATGGTTGTTGCTGTTGAATCTGTACTTCAACAACCCGTTCCTGAGGGTCGTGTCAAGCAGAAGCTATCATCAGGAGGCAAATATGTATCTGTAAACATTGGTCCTGTTCAAGTCATTTCTAGCGAACAGGTCCAAGCTGTATACAATGCAATGAGAAGAGATGACCGGATGAAATACTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

205

Amino Acids

22.48

Weight (kDa)

8.97

Isoelectric Point (pI)

58.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF493 PF04359 122 - 205 2e-20 Protein of unknown function (DUF493)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 342
AccBSI CCGCTC 2 cut(s) 29, 203
AccI GTMKAC 1 cut(s) 573
AciI CCGC 5 cut(s) 27, 116, 127, 201, 227
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 2 cut(s) 156, 321
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 294
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 2 cut(s) 232, 342
AgsI TTSAA 4 cut(s) 136, 449, 461, 542
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 5 cut(s) 72, 121, 251, 496, 569
AluI AGCT 5 cut(s) 72, 121, 251, 496, 569
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 4 cut(s) 156, 223, 237, 321
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 4 cut(s) 165, 224, 533, 562
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 3 cut(s) 165, 533, 562
AxyI CCTNAGG 2 cut(s) 231, 474
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 130
BceAI ACGGC 2 cut(s) 224, 411
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 2 cut(s) 267, 552
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme18I GGWCC 3 cut(s) 165, 533, 562
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 4 cut(s) 165, 224, 533, 562
BmiI GGNNCC 2 cut(s) 167, 406
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 407, 597
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 303, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 342
Bse21I CCTNAGG 2 cut(s) 231, 474
Bse3DI GCAATG 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 421, 606
BseLI CCNNNNNNNGG 2 cut(s) 232, 342
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 187, 245, 465
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 446
BshFI GGCC 4 cut(s) 158, 225, 239, 323
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 2 cut(s) 408, 598
BslI CCNNNNNNNGG 2 cut(s) 232, 342
BsmAI GTCTC 1 cut(s) 138
BsmBI CGTCTC 1 cut(s) 138
BsnI GGCC 4 cut(s) 158, 225, 239, 323
BsoBI CYCGRG 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 1 cut(s) 355
BspACI CCGC 5 cut(s) 27, 116, 127, 201, 227
BspANI GGCC 4 cut(s) 158, 225, 239, 323
BspCNI CTCAG 3 cut(s) 186, 244, 466
BspLI GGNNCC 2 cut(s) 167, 406
BspPI GGATC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 36
BsrBI CCGCTC 2 cut(s) 29, 203
BsrDI GCAATG 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 355
BssNAI GTATAC 1 cut(s) 574
BssNI GRCGYC 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 574
Bst4CI ACNGT 2 cut(s) 319, 382
Bst6I CTCTTC 3 cut(s) 36, 88, 583
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 7, 227, 429
BstDEI CTNAG 3 cut(s) 173, 231, 474
BstF5I GGATG 2 cut(s) 421, 606
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 574
Bsu36I CCTNAGG 2 cut(s) 231, 474
BsuRI GGCC 4 cut(s) 158, 225, 239, 323
BtsCI GGATG 2 cut(s) 421, 606
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 7, 227, 429
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 4 cut(s) 165, 224, 533, 562
Csp6I GTAC 1 cut(s) 455
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 3 cut(s) 173, 231, 474
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
EaeI YGGCCR 2 cut(s) 156, 321
Eam1104I CTCTTC 3 cut(s) 36, 88, 583
EarI CTCTTC 3 cut(s) 36, 88, 583
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 74
Eco47I GGWCC 3 cut(s) 165, 533, 562
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 2 cut(s) 231, 474
Eco88I CYCGRG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 138
FaiI YATR 4 cut(s) 373, 434, 516, 574
FauI CCCGC 4 cut(s) 123, 134, 208, 234
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 2 cut(s) 428, 613
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 2 cut(s) 267, 552
GlaI GCGC 1 cut(s) 23
GluI GCNGC 1 cut(s) 119
HaeIII GGCC 4 cut(s) 158, 225, 239, 323
HapII CCGG 2 cut(s) 408, 598
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HinfI GANTC 3 cut(s) 105, 329, 449
HpaII CCGG 2 cut(s) 408, 598
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 5 cut(s) 58, 151, 393, 526, 574
Hpy188I TCNGA 5 cut(s) 69, 92, 211, 259, 360
Hpy188III TCNNGA 5 cut(s) 136, 233, 267, 473, 504
Hpy8I GTNNAC 5 cut(s) 58, 151, 393, 526, 574
HpyAV CCTTC 2 cut(s) 269, 355
HpyCH4III ACNGT 2 cut(s) 319, 382
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 3 cut(s) 9, 427, 581
HpyF3I CTNAG 3 cut(s) 173, 231, 474
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 355
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 29, 69, 77, 93, 185
LpnPI CCDG 8 cut(s) 218, 413, 421, 486, 489, 545, 549, 611
Lsp1109I GCAGC 1 cut(s) 130
MaeI CTAG 2 cut(s) 267, 552
MaeII ACGT 1 cut(s) 147
MalI GATC 1 cut(s) 357
MbiI CCGCTC 2 cut(s) 29, 203
MboI GATC 1 cut(s) 355
MboII GAAGA 3 cut(s) 23, 75, 600
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 2 cut(s) 131, 367
MluNI TGGCCA 1 cut(s) 158
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 2 cut(s) 408, 598
NdeII GATC 1 cut(s) 355
NlaIV GGNNCC 2 cut(s) 167, 406
OliI CACNNNNGTG 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 105, 329, 449
PflMI CCANNNNNTGG 1 cut(s) 342
PkrI GCNGC 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 167, 406
PspPI GGNCC 4 cut(s) 165, 224, 533, 562
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 36
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 4 cut(s) 165, 224, 533, 562
SduI GDGCHC 1 cut(s) 74
SinI GGWCC 3 cut(s) 165, 533, 562
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 2 cut(s) 131, 367
SsiI CCGC 5 cut(s) 27, 116, 127, 201, 227
SspMI CTAG 2 cut(s) 267, 552
SstI GAGCTC 1 cut(s) 74
TaaI ACNGT 2 cut(s) 319, 382
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 348
TasI AATT 2 cut(s) 131, 367
TatI WGTACW 1 cut(s) 454
TfiI GAWTC 3 cut(s) 105, 329, 449
TscAI CASTG 1 cut(s) 343
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 360, 617
TspGWI ACGGA 3 cut(s) 64, 117, 178
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 342
VpaK11BI GGWCC 3 cut(s) 165, 533, 562
XapI RAATTY 1 cut(s) 367
XbaI TCTAGA 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 2 cut(s) 267, 552
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.