Rorug04G0241600

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005463
Physical Location & Seq
Forward (+)
40995791 .. 40997307
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0241600.1

Sequence Viewer

Length: 1140 bp
ATGGGTGGAAAGAGAGAGTTAATCCGCAAGTCGTGGATGTCACTTAAGGACCTTTGCAGGCGTGAAGCAGACTGGAACTCATATAAGGTCGCTTGTGAGGAAGATGCAGATTTGAAATCCTTTGACGCCGACGTCCAAAGCCGAACCACAAAGGTCATCAGCACCCTTCAGAATGGAGGTGTTTCAACTGATTATGTGAGTGAAGCCATATCATATGTGACAGAATTTAACAAGCAGACTGTGGACGCAATCAGGGCATGCAGAGAAGATATAACGAGTGGTGCGTGGAAGAATAAGAAGAAGAAGATGAAGAGTGAAGAAAGCTTTAAGGTGGTGGTGGAGGAGTACGTCGACAACAGCCAAGAGATGCTGGATTTCTGCTCTGTATTGGACAAGTTCCTGAAATCGGCTCGCAACAACCATTTTGAGATTGAACAAGTAATTCAACAGTGCGAGATGGAGACTGATCCCGAGGGAGGAAATAAGTACGTGAGGATTTCGAGGAAATTGAAGAGTCTCAAGGCTAGTGCACCCCATTACGATTCTGCAAACAGATTACTAGAAAAGATTCAAAGCTTGCAGGCCAAGAATCAAGGGCTGATCGATAAACTGAAAATTGAATATGAGAAGCTGGCAAACAAGCGGAAGAGCGTTCGCGCATGGAAGAAGGCGGTGAATATGATATTCATCACCACAGTGGCTGCCCAAGTTATTTCTGCAGTCGCGGTGCTAGCTATGGCTAATCCAGCTGCTTCTGCAGCTATTGCTGTTACCATTGCCCCAGTAATTGCTGGACATCACTGGGCTCTTGGTTTGCTGACAGAATACGAAAAAAGTTTGAATGATGACAAAGTGATACTGCAAACAATGCATGATGCAAGTGTGTTTTGCATTAAGGAGTTGGGCGACGTAAAGTTTCTCATCGATAAGGTTGTAAATGAGATTGATGCTGTCTTGTTTCACGATTGTCATGCTGATTCTGCCATGGAGGAGGGACAAGTGGAAAACGCTATCAAGAATATTAAGAGTAAGCTGGACATGTTTCTCAAGAAAATTGAGAAATTGGAGGAAAAAGGAGAGACTTGCAGCTCTGAGGTATTGAAATCAAGGGATAAGGTTGTGAGGAGCATGAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

379

Amino Acids

42.67

Weight (kDa)

7.98

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 38 - 374 3.2e-43 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AatII GACGTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 657, 725
AciI CCGC 4 cut(s) 25, 643, 671, 725
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 2 cut(s) 126, 132
AfaI GTAC 2 cut(s) 347, 488
AfiI CCNNNNNNNGG 2 cut(s) 406, 476
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 1038
AgsI TTSAA 9 cut(s) 115, 186, 434, 446, 511, 572, 620, 841, 1102
AleI CACNNNNGTG 1 cut(s) 695
AluBI AGCT 8 cut(s) 324, 576, 631, 734, 749, 761, 1033, 1089
AluI AGCT 8 cut(s) 324, 576, 631, 734, 749, 761, 1033, 1089
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 3 cut(s) 455, 521, 1073
Alw44I GTGCAC 1 cut(s) 528
AlwI GGATC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 582
ApaLI GTGCAC 1 cut(s) 528
ApeKI GCWGC 4 cut(s) 701, 749, 758, 1086
ApoI RAATTY 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 682, 685
AsuNHI GCTAGC 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 808
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 4 cut(s) 688, 736, 770, 1098
BccI CCATC 1 cut(s) 451
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BcoDI GTCTC 3 cut(s) 455, 521, 1073
BfaI CTAG 3 cut(s) 525, 560, 731
BfmI CTRYAG 2 cut(s) 717, 756
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 4 cut(s) 702, 750, 759, 1087
BlsI GCNGC 4 cut(s) 703, 751, 760, 1088
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 49
BmrI ACTGGG 2 cut(s) 776, 811
BmsI GCATC 4 cut(s) 94, 357, 865, 937
BmtI GCTAGC 1 cut(s) 734
BmuI ACTGGG 2 cut(s) 776, 811
BpuEI CTTGAG 2 cut(s) 503, 1031
Bsa29I ATCGAT 2 cut(s) 603, 924
BsaAI YACGTR 1 cut(s) 490
BsaBI GATNNNNATC 1 cut(s) 686
BsaHI GRCGYC 2 cut(s) 126, 132
BsaJI CCNNGG 2 cut(s) 471, 984
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 476
Bse1I ACTGG 3 cut(s) 77, 782, 806
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 686
BseCI ATCGAT 2 cut(s) 603, 924
BseDI CCNNGG 2 cut(s) 471, 984
BseGI GGATG 1 cut(s) 42
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 2 cut(s) 406, 476
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 1 cut(s) 1083
BseNI ACTGG 3 cut(s) 77, 782, 806
BseRI GAGGAG 3 cut(s) 356, 1004, 1138
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 4 cut(s) 688, 736, 770, 1098
Bsh1236I CGCG 2 cut(s) 657, 725
BshFI GGCC 1 cut(s) 584
BshVI ATCGAT 2 cut(s) 603, 924
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 2 cut(s) 406, 476
BsmAI GTCTC 3 cut(s) 455, 521, 1073
BsmFI GGGAC 1 cut(s) 1008
BsnI GGCC 1 cut(s) 584
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 532, 808
Bsp143I GATC 2 cut(s) 466, 600
Bsp19I CCATGG 1 cut(s) 984
BspACI CCGC 4 cut(s) 25, 643, 671, 725
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 1084
BspDI ATCGAT 2 cut(s) 603, 924
BspFNI CGCG 2 cut(s) 657, 725
BspMAI CTGCAG 2 cut(s) 721, 760
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 641
BspTI CTTAAG 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 774
BsrI ACTGG 3 cut(s) 77, 782, 806
BssECI CCNNGG 2 cut(s) 471, 984
BssMI GATC 2 cut(s) 466, 600
BssNI GRCGYC 2 cut(s) 126, 132
BssT1I CCWWGG 1 cut(s) 984
Bst4CI ACNGT 3 cut(s) 241, 450, 697
Bst6I CTCTTC 3 cut(s) 305, 506, 641
BstACI GRCGYC 2 cut(s) 126, 132
BstAFI CTTAAG 1 cut(s) 44
BstAPI GCANNNNNTGC 2 cut(s) 764, 868
BstBAI YACGTR 1 cut(s) 490
BstC8I GCNNGC 7 cut(s) 59, 259, 412, 578, 582, 633, 732
BstDEI CTNAG 1 cut(s) 1092
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 1 cut(s) 42
BstFNI CGCG 2 cut(s) 657, 725
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 2 cut(s) 469, 603
BstMAI GTCTC 3 cut(s) 455, 521, 1073
BstMBI GATC 2 cut(s) 466, 600
BstMWI GCNNNNNNNGC 8 cut(s) 254, 731, 746, 755, 758, 764, 868, 980
BstNSI RCATGY 2 cut(s) 261, 1042
BstSFI CTRYAG 2 cut(s) 717, 756
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 2 cut(s) 657, 725
BstV1I GCAGC 4 cut(s) 688, 736, 770, 1098
Bsu15I ATCGAT 2 cut(s) 603, 924
BsuRI GGCC 1 cut(s) 584
BsuTUI ATCGAT 2 cut(s) 603, 924
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 3 cut(s) 455, 702, 799
Cac8I GCNNGC 7 cut(s) 59, 259, 412, 578, 582, 633, 732
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 49
ClaI ATCGAT 2 cut(s) 603, 924
CseI GACGC 2 cut(s) 134, 254
Csp6I GTAC 2 cut(s) 346, 487
CviAII CATG 7 cut(s) 258, 660, 872, 971, 985, 1039, 1129
CviQI GTAC 2 cut(s) 346, 487
DdeI CTNAG 1 cut(s) 1092
DpnI GATC 2 cut(s) 468, 602
DpnII GATC 2 cut(s) 466, 600
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 305, 506, 641
EarI CTCTTC 3 cut(s) 305, 506, 641
Eco130I CCWWGG 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 808
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 1 cut(s) 873
EcoT38I GRGCYC 1 cut(s) 808
ErhI CCWWGG 1 cut(s) 984
FaeI CATG 7 cut(s) 261, 663, 875, 974, 988, 1042, 1132
FaqI GGGAC 1 cut(s) 1008
FatI CATG 7 cut(s) 257, 659, 871, 970, 984, 1038, 1128
FauNDI CATATG 1 cut(s) 214
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 702, 750, 759, 1087
FokI GGATG 1 cut(s) 49
FriOI GRGCYC 1 cut(s) 808
Fsp4HI GCNGC 4 cut(s) 702, 750, 759, 1087
FspBI CTAG 3 cut(s) 525, 560, 731
GlaI GCGC 1 cut(s) 658
GluI GCNGC 4 cut(s) 702, 750, 759, 1087
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 2 cut(s) 134, 254
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 2 cut(s) 126, 132
Hin1II CATG 7 cut(s) 261, 663, 875, 974, 988, 1042, 1132
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 2 cut(s) 322, 574
HinfI GANTC 5 cut(s) 514, 542, 568, 589, 977
HphI GGTGA 2 cut(s) 682, 685
Hpy166II GTNNAC 3 cut(s) 244, 352, 530
Hpy188I TCNGA 2 cut(s) 171, 1093
Hpy188III TCNNGA 5 cut(s) 400, 470, 962, 1015, 1048
Hpy8I GTNNAC 3 cut(s) 244, 352, 530
Hpy99I CGWCG 3 cut(s) 134, 353, 911
HpyAV CCTTC 2 cut(s) 176, 661
HpyCH4III ACNGT 3 cut(s) 241, 450, 697
HpyCH4IV ACGT 4 cut(s) 132, 348, 489, 909
HpyF10VI GCNNNNNNNGC 8 cut(s) 254, 731, 746, 755, 758, 764, 868, 980
HpyF3I CTNAG 1 cut(s) 1092
HpySE526I ACGT 4 cut(s) 132, 348, 489, 909
Hsp92I GRCGYC 2 cut(s) 126, 132
Hsp92II CATG 7 cut(s) 261, 663, 875, 974, 988, 1042, 1132
HspAI GCGC 1 cut(s) 657
Kzo9I GATC 2 cut(s) 466, 600
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 1 cut(s) 1125
Lsp1109I GCAGC 4 cut(s) 688, 736, 770, 1098
LweI GCATC 4 cut(s) 94, 357, 865, 937
MaeI CTAG 3 cut(s) 525, 560, 731
MaeII ACGT 4 cut(s) 132, 348, 489, 909
MaeIII GTNAC 3 cut(s) 39, 217, 769
MalI GATC 2 cut(s) 468, 602
MboI GATC 2 cut(s) 466, 600
MhlI GDGCHC 2 cut(s) 532, 808
MluCI AATT 7 cut(s) 224, 441, 506, 615, 786, 1053, 1061
MlyI GAGTC 1 cut(s) 523
Mph1103I ATGCAT 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 6 cut(s) 20, 45, 228, 327, 894, 1023
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 44
MvnI CGCG 2 cut(s) 657, 725
MwoI GCNNNNNNNGC 8 cut(s) 254, 731, 746, 755, 758, 764, 868, 980
NcoI CCATGG 1 cut(s) 984
NdeI CATATG 1 cut(s) 214
NdeII GATC 2 cut(s) 466, 600
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 7 cut(s) 261, 663, 875, 974, 988, 1042, 1132
NmuCI GTSAC 2 cut(s) 39, 217
NsiI ATGCAT 1 cut(s) 873
NspI RCATGY 2 cut(s) 261, 1042
OliI CACNNNNGTG 1 cut(s) 695
PaeI GCATGC 1 cut(s) 261
PciI ACATGT 1 cut(s) 1038
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 4 cut(s) 542, 568, 589, 977
PkrI GCNGC 4 cut(s) 703, 751, 760, 1088
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Ppu21I YACGTR 1 cut(s) 490
PpuMI RGGWCCY 1 cut(s) 49
PscI ACATGT 1 cut(s) 1038
Psp5II RGGWCCY 1 cut(s) 49
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
PstI CTGCAG 2 cut(s) 721, 760
PstNI CAGNNNCTG 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 2 cut(s) 347, 488
RsaNI GTAC 2 cut(s) 346, 487
RseI CAYNNNNRTG 1 cut(s) 695
SalI GTCGAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 6 cut(s) 20, 45, 228, 327, 894, 1023
SatI GCNGC 4 cut(s) 702, 750, 759, 1087
Sau3AI GATC 2 cut(s) 466, 600
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 523
SduI GDGCHC 2 cut(s) 532, 808
SfaNI GCATC 4 cut(s) 94, 357, 865, 937
SfcI CTRYAG 2 cut(s) 717, 756
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 3 cut(s) 44, 518, 1046
SmoI CTYRAG 3 cut(s) 44, 518, 1046
SphI GCATGC 1 cut(s) 261
Sse9I AATT 7 cut(s) 224, 441, 506, 615, 786, 1053, 1061
SsiI CCGC 4 cut(s) 25, 643, 671, 725
SspI AATATT 1 cut(s) 1021
SspMI CTAG 3 cut(s) 525, 560, 731
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 3 cut(s) 241, 450, 697
TaiI ACGT 4 cut(s) 135, 351, 492, 912
TaqI TCGA 4 cut(s) 351, 500, 603, 924
TasI AATT 7 cut(s) 224, 441, 506, 615, 786, 1053, 1061
TfiI GAWTC 4 cut(s) 542, 568, 589, 977
Tru1I TTAA 6 cut(s) 20, 45, 228, 327, 894, 1023
Tru9I TTAA 6 cut(s) 20, 45, 228, 327, 894, 1023
TscAI CASTG 3 cut(s) 455, 702, 806
TseFI GTSAC 2 cut(s) 39, 217
TseI GCWGC 4 cut(s) 701, 749, 758, 1086
Tsp45I GTSAC 2 cut(s) 39, 217
TspDTI ATGAA 2 cut(s) 323, 676
TspRI CASTG 3 cut(s) 455, 702, 806
Vha464I CTTAAG 1 cut(s) 44
VneI GTGCAC 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 224
XceI RCATGY 2 cut(s) 261, 1042
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 3 cut(s) 525, 560, 731
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.