MD13G1119600.v1.1

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005463
Physical Location & Seq
Forward (+)
8762812 .. 8766058
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1119600.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCGTGCAGGACTGCGCTACGATCCGCTCTGCTAATAGAAGCGTGGCGACCCGTTCATTTCGCTCGGAGCTCCGCCTCCGCCTCGTTCAGGACCCGCGGGTTCTCCTCCCCCAGGCTGAGCCGCCACTTCAGGACCCGGGACATCCACTTCGCGCGACGGACTCACCTCAACCGCGCCCACAACGAGAACTCGTCGGATTTGTCGGAGGACGACCAGGGCCCGCCGCAAGAGGCCATCTTGAAAGCAATTTCAGAGGTTTCAAAGACTGAAGGGAGAGTTGGGCAGACGACGAATGTGGTTATCGGAGGCACGGTGGCGGATGATTCTACGAATGAATGGGTCGACTTGGATCAGAAGGTAAATTCGTATCCAACTGTTAGAGGGTTCACAGCAATTGGAACTGGAGGGGATGATTTTGTGCAAGCTATGGTTGTTGCCGTCGAATCCGTAATTCAACAACCGGTCCCTGAGGGTCATGTGAGGCAGAAGGTATCATCAAGGGGAAAATATGTATCTGTAAACATTGGACCCGTTCAAGTAATTTCAAGTGAACAGGTCCAAGCTGTATACAATGCCATGAGAAGAGATGATCGGATGAAATACTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

205

Amino Acids

22.64

Weight (kDa)

9.45

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF493 PF04359 121 - 204 3.7e-20 Protein of unknown function (DUF493)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 29
AccI GTMKAC 2 cut(s) 345, 570
AccII CGCG 4 cut(s) 99, 155, 157, 177
AclWI GGATC 2 cut(s) 18, 360
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 2 cut(s) 115, 291
AfiI CCNNNNNNNGG 2 cut(s) 90, 114
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 5 cut(s) 244, 264, 458, 539, 549
AjnI CCWGG 2 cut(s) 113, 216
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 3 cut(s) 72, 428, 566
AluI AGCT 3 cut(s) 72, 428, 566
Alw21I GWGCWC 1 cut(s) 74
AlwI GGATC 2 cut(s) 18, 360
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 220, 234
ApaI GGGCCC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AsiGI ACCGGT 1 cut(s) 463
AspLEI GCGC 3 cut(s) 19, 157, 179
AspS9I GGNCC 7 cut(s) 93, 135, 220, 221, 466, 530, 559
AsuC2I CCSGG 2 cut(s) 139, 140
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 5 cut(s) 93, 135, 466, 530, 559
AxyI CCTNAGG 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 224
BanII GRGCYC 2 cut(s) 74, 224
Bbv12I GWGCWC 1 cut(s) 74
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 425
BciT130I CCWGG 2 cut(s) 115, 218
BciVI GTATCC 1 cut(s) 381
BcnI CCSGG 2 cut(s) 139, 140
BfuI GTATCC 1 cut(s) 381
BisI GCNGC 2 cut(s) 124, 227
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 2 cut(s) 125, 228
Bme1390I CCNGG 4 cut(s) 115, 139, 140, 218
Bme18I GGWCC 5 cut(s) 93, 135, 466, 530, 559
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 7 cut(s) 93, 135, 220, 221, 466, 530, 559
BmiI GGNNCC 5 cut(s) 95, 137, 222, 468, 532
BmrFI CCNGG 4 cut(s) 115, 139, 140, 218
BpmI CTGGAG 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 139, 140
BsaJI CCNNGG 4 cut(s) 97, 113, 138, 217
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 114
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 409
Bse21I CCTNAGG 1 cut(s) 471
BseBI CCWGG 2 cut(s) 115, 218
BseDI CCNNGG 4 cut(s) 97, 113, 138, 217
BseGI GGATG 4 cut(s) 144, 328, 418, 603
BseLI CCNNNNNNNGG 2 cut(s) 90, 114
BseMII CTCAG 2 cut(s) 110, 462
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 224
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 4 cut(s) 99, 155, 157, 177
BshFI GGCC 2 cut(s) 222, 236
BshTI ACCGGT 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 2 cut(s) 139, 464
BslFI GGGAC 2 cut(s) 155, 452
BslI CCNNNNNNNGG 2 cut(s) 90, 114
BsmFI GGGAC 2 cut(s) 155, 452
BsnI GGCC 2 cut(s) 222, 236
BsoBI CYCGRG 1 cut(s) 138
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 74, 224
Bsp143I GATC 3 cut(s) 23, 352, 592
Bsp1720I GCTNAGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 222, 236
BspCNI CTCAG 2 cut(s) 111, 463
BspFNI CGCG 4 cut(s) 99, 155, 157, 177
BspLI GGNNCC 5 cut(s) 95, 137, 222, 468, 532
BspPI GGATC 2 cut(s) 18, 360
BsrBI CCGCTC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 4 cut(s) 97, 113, 138, 217
BssMI GATC 3 cut(s) 23, 352, 592
BssNAI GTATAC 1 cut(s) 571
Bst1107I GTATAC 1 cut(s) 571
Bst2UI CCWGG 2 cut(s) 115, 218
Bst4CI ACNGT 2 cut(s) 316, 379
Bst6I CTCTTC 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 7, 224, 426
BstDEI CTNAG 2 cut(s) 119, 471
BstDSI CCRYGG 1 cut(s) 97
BstENI CCTNNNNNAGG 2 cut(s) 88, 112
BstF5I GGATG 4 cut(s) 144, 328, 418, 603
BstFNI CGCG 4 cut(s) 99, 155, 157, 177
BstHHI GCGC 3 cut(s) 19, 157, 179
BstKTI GATC 3 cut(s) 26, 355, 595
BstMBI GATC 3 cut(s) 23, 352, 592
BstNI CCWGG 2 cut(s) 115, 218
BstSCI CCNGG 4 cut(s) 113, 137, 138, 216
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 4 cut(s) 99, 155, 157, 177
BstZ17I GTATAC 1 cut(s) 571
Bsu36I CCTNAGG 1 cut(s) 471
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 2 cut(s) 222, 236
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 4 cut(s) 144, 328, 418, 603
Cac8I GCNNGC 3 cut(s) 7, 224, 426
CfoI GCGC 3 cut(s) 19, 157, 179
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 7 cut(s) 93, 135, 220, 221, 466, 530, 559
Cfr42I CCGCGG 1 cut(s) 100
Cfr9I CCCGGG 1 cut(s) 138
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 2 cut(s) 479, 580
CviJI RGCY 7 cut(s) 72, 118, 123, 222, 236, 428, 566
CviKI_1 RGCY 7 cut(s) 72, 118, 123, 222, 236, 428, 566
DdeI CTNAG 2 cut(s) 119, 471
DpnI GATC 3 cut(s) 25, 354, 594
DpnII GATC 3 cut(s) 23, 352, 592
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
EciI GGCGGA 3 cut(s) 64, 70, 335
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 74, 224
Eco47I GGWCC 5 cut(s) 93, 135, 466, 530, 559
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 115, 291
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 72
EcoNI CCTNNNNNAGG 2 cut(s) 88, 112
EcoO109I RGGNCCY 3 cut(s) 93, 135, 220
EcoRII CCWGG 2 cut(s) 113, 216
EcoT38I GRGCYC 2 cut(s) 74, 224
FaeI CATG 2 cut(s) 482, 583
FaiI YATR 5 cut(s) 431, 480, 513, 571, 581
FaqI GGGAC 2 cut(s) 155, 452
FatI CATG 2 cut(s) 478, 579
FauI CCCGC 3 cut(s) 92, 104, 231
FblI GTMKAC 2 cut(s) 345, 570
Fnu4HI GCNGC 2 cut(s) 124, 227
FokI GGATG 4 cut(s) 131, 335, 425, 610
FriOI GRGCYC 2 cut(s) 74, 224
Fsp4HI GCNGC 2 cut(s) 124, 227
GlaI GCGC 3 cut(s) 18, 156, 178
GluI GCNGC 2 cut(s) 124, 227
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 2 cut(s) 222, 236
HapII CCGG 2 cut(s) 139, 464
HhaI GCGC 3 cut(s) 19, 157, 179
Hin1II CATG 2 cut(s) 482, 583
Hin6I GCGC 3 cut(s) 17, 155, 177
HinP1I GCGC 3 cut(s) 17, 155, 177
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 163, 326, 446
HpaII CCGG 2 cut(s) 139, 464
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 5 cut(s) 346, 390, 523, 554, 571
Hpy188I TCNGA 7 cut(s) 69, 199, 208, 256, 308, 357, 597
Hpy188III TCNNGA 3 cut(s) 91, 133, 241
Hpy8I GTNNAC 5 cut(s) 346, 390, 523, 554, 571
Hpy99I CGWCG 4 cut(s) 162, 199, 295, 446
HpyAV CCTTC 3 cut(s) 266, 352, 484
HpyCH4III ACNGT 2 cut(s) 316, 379
HpyCH4V TGCA 2 cut(s) 9, 424
HpyF3I CTNAG 2 cut(s) 119, 471
Hsp92II CATG 2 cut(s) 482, 583
HspAI GCGC 3 cut(s) 17, 155, 177
KspI CCGCGG 1 cut(s) 100
Kzo9I GATC 3 cut(s) 23, 352, 592
LmnI GCTCC 2 cut(s) 69, 77
MalI GATC 3 cut(s) 25, 354, 594
MbiI CCGCTC 1 cut(s) 29
MboI GATC 3 cut(s) 23, 352, 592
MboII GAAGA 1 cut(s) 597
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 74, 224
MluCI AATT 5 cut(s) 249, 364, 396, 453, 543
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 3 cut(s) 177, 186, 398
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 2 cut(s) 139, 464
MspR9I CCNGG 4 cut(s) 115, 139, 140, 218
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 2 cut(s) 115, 218
MvnI CGCG 4 cut(s) 99, 155, 157, 177
NciI CCSGG 2 cut(s) 139, 140
NdeII GATC 3 cut(s) 23, 352, 592
NlaIII CATG 2 cut(s) 482, 583
NlaIV GGNNCC 5 cut(s) 95, 137, 222, 468, 532
PfeI GAWTC 2 cut(s) 326, 446
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 2 cut(s) 125, 228
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PpuMI RGGWCCY 2 cut(s) 93, 135
Psp124BI GAGCTC 1 cut(s) 74
Psp5II RGGWCCY 2 cut(s) 93, 135
Psp6I CCWGG 2 cut(s) 113, 216
PspGI CCWGG 2 cut(s) 113, 216
PspN4I GGNNCC 5 cut(s) 95, 137, 222, 468, 532
PspOMI GGGCCC 1 cut(s) 220
PspPI GGNCC 7 cut(s) 93, 135, 220, 221, 466, 530, 559
PspPPI RGGWCCY 2 cut(s) 93, 135
SacI GAGCTC 1 cut(s) 74
SacII CCGCGG 1 cut(s) 100
SalI GTCGAC 1 cut(s) 344
SatI GCNGC 2 cut(s) 124, 227
Sau3AI GATC 3 cut(s) 23, 352, 592
Sau96I GGNCC 7 cut(s) 93, 135, 220, 221, 466, 530, 559
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 4 cut(s) 115, 139, 140, 218
SduI GDGCHC 2 cut(s) 74, 224
SetI ASST 8 cut(s) 74, 171, 261, 363, 430, 495, 561, 568
Sfr303I CCGCGG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 100
SinI GGWCC 5 cut(s) 93, 135, 466, 530, 559
SmaI CCCGGG 1 cut(s) 140
Sse9I AATT 5 cut(s) 249, 364, 396, 453, 543
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 4 cut(s) 113, 137, 138, 216
TaaI ACNGT 2 cut(s) 316, 379
TaqI TCGA 2 cut(s) 345, 444
TasI AATT 5 cut(s) 249, 364, 396, 453, 543
TauI GCSGC 2 cut(s) 126, 229
TfiI GAWTC 2 cut(s) 326, 446
TspDTI ATGAA 3 cut(s) 47, 351, 614
TspGWI ACGGA 2 cut(s) 175, 439
TspMI CCCGGG 1 cut(s) 138
VpaK11BI GGWCC 5 cut(s) 93, 135, 466, 530, 559
XagI CCTNNNNNAGG 2 cut(s) 88, 112
XapI RAATTY 1 cut(s) 364
XmaI CCCGGG 1 cut(s) 138
XmiI GTMKAC 2 cut(s) 345, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.