MD16G1119800.v1.1

Protein of unknown function (DUF493)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005463
Physical Location & Seq
Forward (+)
8590773 .. 8594082
3310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1119800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCGTGCAGGACCGCGCTCCGCTCCGCTCTGCTAATAGAAGCGTGGCGACCCGTTCATCTCGCTCGGAGCTCCGCCTCCGCCTCGTTCAGGACCCGCGGATTCTCCTCCCCCAGGCTGAGCCGCCACTTCAGGACCCGAGACATCCACTTCGCGCGACGGACTCACCTCAACAGCTCCCACAACGAAAACTCGCCGGCTTTGTCGGAGGACGACCAGGGCCCGCCGCAAGAGGCCATCTTGAAAGCAATTTCAGAGGTGTCGAAAACAGAAGGGAGGGTTGGGCAGACGACGAATGTGGTTATCGGAGGCACGGTGGCTGATGATTCTACGAATGAATGGGTCGACTTGGATCAAAAGGTAAATTCGTACCCGACTGTTAGAGGGTTCACAGCAATTGGAACTGGAGGGGATGATTTTGTGCAAGCTATGGTTGTTGCTGTCGAGTCCGTAATTCAGCAACCTGTTCCAGAGGGTCGTGTGAGGCAGAAGGTATCATCAAGGGGGAAATATGTATCTGTAAACATTGGACCTGTTCAAGTAATTTCTAGCGAACAGGTCCAAGCTGTGTACAATGCCATGAGAAGAGATGATCGGATGAAATACTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF493

Protein Analysis

205

Amino Acids

22.54

Weight (kDa)

9.65

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF493 PF04359 121 - 204 4e-20 Protein of unknown function (DUF493)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 24, 29
AccI GTMKAC 1 cut(s) 345
AccII CGCG 4 cut(s) 17, 99, 155, 157
AclWI GGATC 1 cut(s) 360
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 115
AfaI GTAC 2 cut(s) 371, 572
AfiI CCNNNNNNNGG 2 cut(s) 90, 114
AgsI TTSAA 2 cut(s) 244, 539
AjnI CCWGG 2 cut(s) 113, 216
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 4 cut(s) 72, 177, 428, 566
AluI AGCT 4 cut(s) 72, 177, 428, 566
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 220, 234
ApaI GGGCCC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspLEI GCGC 2 cut(s) 19, 157
AspS9I GGNCC 7 cut(s) 12, 93, 135, 220, 221, 530, 559
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 5 cut(s) 12, 93, 135, 530, 559
BaeGI GKGCMC 1 cut(s) 224
BanII GRGCYC 2 cut(s) 74, 224
Bbv12I GWGCWC 1 cut(s) 74
BccI CCATC 1 cut(s) 245
BciT130I CCWGG 2 cut(s) 115, 218
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 549
BisI GCNGC 2 cut(s) 124, 227
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 2 cut(s) 125, 228
Bme1390I CCNGG 2 cut(s) 115, 218
Bme18I GGWCC 5 cut(s) 12, 93, 135, 530, 559
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 7 cut(s) 12, 93, 135, 220, 221, 530, 559
BmiI GGNNCC 3 cut(s) 95, 137, 222
BmrFI CCNGG 2 cut(s) 115, 218
BpmI CTGGAG 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 97, 113, 217
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 114
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 2 cut(s) 115, 218
BseDI CCNNGG 3 cut(s) 97, 113, 217
BseGI GGATG 3 cut(s) 144, 418, 603
BseLI CCNNNNNNNGG 2 cut(s) 90, 114
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 224
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 4 cut(s) 17, 99, 155, 157
BshFI GGCC 2 cut(s) 222, 236
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 90, 114
BsmAI GTCTC 1 cut(s) 135
BsnI GGCC 2 cut(s) 222, 236
BsoBI CYCGRG 1 cut(s) 138
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 74, 224
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 2 cut(s) 352, 592
Bsp1720I GCTNAGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 222, 236
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 4 cut(s) 17, 99, 155, 157
BspLI GGNNCC 3 cut(s) 95, 137, 222
BspPI GGATC 1 cut(s) 360
BsrBI CCGCTC 2 cut(s) 24, 29
BsrFI RCCGGY 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 3 cut(s) 97, 113, 217
BssMI GATC 2 cut(s) 352, 592
Bst2UI CCWGG 2 cut(s) 115, 218
Bst4CI ACNGT 2 cut(s) 316, 379
Bst6I CTCTTC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 570
BstC8I GCNNGC 4 cut(s) 7, 198, 224, 426
BstDEI CTNAG 1 cut(s) 119
BstDSI CCRYGG 1 cut(s) 97
BstENI CCTNNNNNAGG 2 cut(s) 88, 112
BstF5I GGATG 3 cut(s) 144, 418, 603
BstFNI CGCG 4 cut(s) 17, 99, 155, 157
BstHHI GCGC 2 cut(s) 19, 157
BstKTI GATC 2 cut(s) 355, 595
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 352, 592
BstNI CCWGG 2 cut(s) 115, 218
BstSCI CCNGG 2 cut(s) 113, 216
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 4 cut(s) 17, 99, 155, 157
BsuRI GGCC 2 cut(s) 222, 236
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 3 cut(s) 144, 418, 603
Cac8I GCNNGC 4 cut(s) 7, 198, 224, 426
CfoI GCGC 2 cut(s) 19, 157
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 7 cut(s) 12, 93, 135, 220, 221, 530, 559
Cfr42I CCGCGG 1 cut(s) 100
Csp6I GTAC 2 cut(s) 370, 571
CviAII CATG 1 cut(s) 580
CviQI GTAC 2 cut(s) 370, 571
DdeI CTNAG 1 cut(s) 119
DpnI GATC 2 cut(s) 354, 594
DpnII GATC 2 cut(s) 352, 592
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
EciI GGCGGA 2 cut(s) 64, 70
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 74, 224
Eco47I GGWCC 5 cut(s) 12, 93, 135, 530, 559
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 72
EcoNI CCTNNNNNAGG 2 cut(s) 88, 112
EcoO109I RGGNCCY 3 cut(s) 93, 135, 220
EcoRII CCWGG 2 cut(s) 113, 216
EcoT38I GRGCYC 2 cut(s) 74, 224
FaeI CATG 1 cut(s) 583
FaiI YATR 3 cut(s) 431, 513, 581
FatI CATG 1 cut(s) 579
FauI CCCGC 2 cut(s) 104, 231
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 2 cut(s) 124, 227
FokI GGATG 3 cut(s) 131, 425, 610
FriOI GRGCYC 2 cut(s) 74, 224
Fsp4HI GCNGC 2 cut(s) 124, 227
FspBI CTAG 1 cut(s) 549
GlaI GCGC 2 cut(s) 18, 156
GluI GCNGC 2 cut(s) 124, 227
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 2 cut(s) 222, 236
HapII CCGG 1 cut(s) 197
HhaI GCGC 2 cut(s) 19, 157
Hin1II CATG 1 cut(s) 583
Hin6I GCGC 2 cut(s) 17, 155
HinP1I GCGC 2 cut(s) 17, 155
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 4 cut(s) 102, 163, 326, 446
HpaII CCGG 1 cut(s) 197
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 4 cut(s) 346, 390, 523, 571
Hpy188I TCNGA 5 cut(s) 69, 208, 256, 308, 597
Hpy188III TCNNGA 4 cut(s) 91, 133, 241, 470
Hpy8I GTNNAC 4 cut(s) 346, 390, 523, 571
Hpy99I CGWCG 2 cut(s) 162, 295
HpyAV CCTTC 2 cut(s) 266, 484
HpyCH4III ACNGT 2 cut(s) 316, 379
HpyCH4V TGCA 2 cut(s) 9, 424
HpyF3I CTNAG 1 cut(s) 119
Hsp92II CATG 1 cut(s) 583
HspAI GCGC 2 cut(s) 17, 155
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 100
Kzo9I GATC 2 cut(s) 352, 592
LmnI GCTCC 5 cut(s) 24, 29, 69, 77, 182
MaeI CTAG 1 cut(s) 549
MalI GATC 2 cut(s) 354, 594
MbiI CCGCTC 2 cut(s) 24, 29
MboI GATC 2 cut(s) 352, 592
MboII GAAGA 1 cut(s) 597
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 74, 224
MluCI AATT 5 cut(s) 249, 364, 396, 453, 543
MlyI GAGTC 2 cut(s) 157, 455
MmeI TCCRAC 1 cut(s) 186
MroNI GCCGGC 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 115, 218
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 2 cut(s) 115, 218
MvnI CGCG 4 cut(s) 17, 99, 155, 157
NaeI GCCGGC 1 cut(s) 198
NdeII GATC 2 cut(s) 352, 592
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 1 cut(s) 583
NlaIV GGNNCC 3 cut(s) 95, 137, 222
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 102, 326
PkrI GCNGC 2 cut(s) 125, 228
PleI GAGTC 2 cut(s) 157, 454
PpsI GAGTC 2 cut(s) 157, 454
PpuMI RGGWCCY 2 cut(s) 93, 135
Psp124BI GAGCTC 1 cut(s) 74
Psp5II RGGWCCY 2 cut(s) 93, 135
Psp6I CCWGG 2 cut(s) 113, 216
PspGI CCWGG 2 cut(s) 113, 216
PspN4I GGNNCC 3 cut(s) 95, 137, 222
PspOMI GGGCCC 1 cut(s) 220
PspPI GGNCC 7 cut(s) 12, 93, 135, 220, 221, 530, 559
PspPPI RGGWCCY 2 cut(s) 93, 135
RsaI GTAC 2 cut(s) 371, 572
RsaNI GTAC 2 cut(s) 370, 571
SacI GAGCTC 1 cut(s) 74
SacII CCGCGG 1 cut(s) 100
SalI GTCGAC 1 cut(s) 344
SatI GCNGC 2 cut(s) 124, 227
Sau3AI GATC 2 cut(s) 352, 592
Sau96I GGNCC 7 cut(s) 12, 93, 135, 220, 221, 530, 559
SchI GAGTC 2 cut(s) 157, 455
ScrFI CCNGG 2 cut(s) 115, 218
SduI GDGCHC 2 cut(s) 74, 224
Sfr303I CCGCGG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 100
SinI GGWCC 5 cut(s) 12, 93, 135, 530, 559
Sse9I AATT 5 cut(s) 249, 364, 396, 453, 543
SspMI CTAG 1 cut(s) 549
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 113, 216
TaaI ACNGT 2 cut(s) 316, 379
TaqI TCGA 3 cut(s) 263, 345, 444
TasI AATT 5 cut(s) 249, 364, 396, 453, 543
TatI WGTACW 1 cut(s) 570
TauI GCSGC 2 cut(s) 126, 229
TfiI GAWTC 2 cut(s) 102, 326
TspDTI ATGAA 3 cut(s) 47, 351, 614
TspGWI ACGGA 2 cut(s) 175, 439
VpaK11BI GGWCC 5 cut(s) 12, 93, 135, 530, 559
XagI CCTNNNNNAGG 2 cut(s) 88, 112
XapI RAATTY 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.