Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005715

Physical Location & Seq

Genomic Coordinates Reverse (-)

9860291 .. 9861568

Gene Structure 1278 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G28220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGTGAAGGCTCTTGTGATCATAAACTGCATAATTCTAGCCATAGGAAACTGTGGAGGTCCTTTGATTATGCGTCTCTACTTCAACAATGGCGGTAAAAGGATTTGGTTCTCTACGTTTCTTGAAACTGCAGGCTTTCCTGTTATCTTCATTCCTCTGCTCTTCTCTTACATTACCCGGCGCAGAAGCAACAATGTGGGTGATAGTACAAGTTTCTTTCTTATCAAACCGCGTCTTCTTATCGCCGCTGTTATTGTAGGCATTCTCTCAGGGTTTGATAACTACTTGTATGCATATGGTATAGCTTATCTTCCAGTTTCTACAGCTGCTCTTATCATTGCTTCACAGTTAGCTTTTATAGCTATCTTCTCATTCTTCATGGTTAAACATAAGTTCACTCCTTTTACCATCAATGCTGTTGTGTTGTTGACTGTTGGTGCTGCGGTTTTGGGAATGCATACCGAAACTGATAAGCCAGTTCATGAGACTCACAAGCAGTACATAACTGGTTTCTTGATTACTGTAGCAGCAGCTGTTATGTATGCTTTCATCTTGCCATTAGTGGAACTTGCTTACCAGAAAGCTAAGCAAACCATGAGCTATACCCTTGTGCTCGAGTTCCAGTTGATTTTGTGTCTCCTTGCTTCTATTGTCAGCGTCATCGGTATGTTCATCGCTGGTGATTTCAAGGCCTTACCAAAAGAAGCAAGAGAGTTCAAGCTTGGAGAGGCATTGTTCTATGTGGTGGCTGTGTTTTCAGCCATCATATGGCAAGGCTTCTTCTTGGGAGCCATTGGATTAATCTTCTCCACATCGTCTCTCGTCTCGGGTATTATGATATCAGTGCTTTTGCCAATTACAGAGGTTTTAGCTGTTATATTCTACCATGAAAAGTTTCAAGCTGAGAAGGGACTTTCTCTTGCTCTCTCCCTTTGGGGCTTTGTCTCTTACTTTTATGGTGAGATAAAGTCTGGCGAGGATAAAAGGAGAATTCAGCAGGAGGAGAGTCAGGAGACAGAACAATCTTCTTTGTCAAGACCCATAAGTGAGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005337 GO:0005345 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006863 GO:0008150 GO:0009914 GO:0010184 GO:0010817 GO:0015205 GO:0015211 GO:0015851 GO:0015858 GO:0015860 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071944 GO:0072530 GO:1901264 GO:1901505 GO:1901642 GO:1904823

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUNUT

Protein Analysis

351

Amino Acids

38.87

Weight (kDa)

8.54

Isoelectric Point (pI)

42.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUNUT	PF16913	5 - 326	4.1e-129	Purine nucleobase transmembrane transport
EamA	PF00892	53 - 150	1.8e-08	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005715)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28220 AT1G28230
fragaria_vesca	FvH4_5g36040 FvH4_5g36060
malus_domestica	MD00G1135600.v1.1 MD15G1379500.v1.1
prunus_persica	Prupe.1G525600_v2.0.a1 Prupe.1G525800_v2.0.a1
pyrus_communis	pycom08g16430 pycom15g33980 pycom15g34010
rosa_chinensis	RchiOBHm_Chr7g0237771 RchiOBHm_Chr7g0237851
rosa_laevigata	RLG00000000962 RLG00000000968
rosa_multiflora	Rmu_ssc0000067.1_g000004 Rmu_ssc0000067.1_g000031
rosa_roxburghii	Rroxscaffold_3G00223710 Rroxscaffold_3G00223760
rosa_rugosa	Rorug07G0308600
rosa_samantha	Rh7AG464000 Rh7BG434100 Rh7CG481000 Rh7CG481500 Rh7DG449900 Rh7DG450200
rosa_wichuraiana	Rw7G038390 Rw7G038400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	768
AccII	CGCG	1 cut(s)	232
AciI	CCGC	4 cut(s)	93, 230, 246, 443
AcsI	RAATTY	1 cut(s)	990
AfaI	GTAC	2 cut(s)	208, 500
AfiI	CCNNNNNNNGG	1 cut(s)	768
AgsI	TTSAA	5 cut(s)	85, 125, 688, 718, 899
Alw21I	GWGCWC	1 cut(s)	615
Alw26I	GTCTC	7 cut(s)	80, 479, 641, 822, 829, 949, 1007
AlwNI	CAGNNNCTG	1 cut(s)	533
Ama87I	CYCGRG	2 cut(s)	614, 826
AoxI	GGCC	1 cut(s)	690
ApeKI	GCWGC	4 cut(s)	326, 440, 527, 530
ApoI	RAATTY	1 cut(s)	990
AseI	ATTAAT	1 cut(s)	800
Asp700I	GAANNNNTTC	1 cut(s)	894
AspLEI	GCGC	1 cut(s)	183
AspS9I	GGNCC	1 cut(s)	59
AsuC2I	CCSGG	1 cut(s)	178
AsuHPI	GGTGA	4 cut(s)	16, 212, 692, 971
AvaI	CYCGRG	2 cut(s)	614, 826
AvaII	GGWCC	1 cut(s)	59
BbsI	GAAGAC	1 cut(s)	227
Bbv12I	GWGCWC	1 cut(s)	615
BbvI	GCAGC	4 cut(s)	313, 427, 539, 542
BccI	CCATC	2 cut(s)	416, 770
BclI	TGATCA	1 cut(s)	18
BcnI	CCSGG	1 cut(s)	178
BcoDI	GTCTC	7 cut(s)	80, 479, 641, 822, 829, 949, 1007
BfaI	CTAG	1 cut(s)	38
BfmI	CTRYAG	3 cut(s)	129, 321, 522
BisI	GCNGC	5 cut(s)	246, 327, 441, 528, 531
BlpI	GCTNAGC	1 cut(s)	585
BlsI	GCNGC	5 cut(s)	247, 328, 442, 529, 532
Bme1390I	CCNGG	1 cut(s)	178
Bme18I	GGWCC	1 cut(s)	59
BmeT110I	CYCGRG	2 cut(s)	614, 826
BmgT120I	GGNCC	1 cut(s)	59
BmiI	GGNNCC	1 cut(s)	790
BmrFI	CCNGG	1 cut(s)	178
BpiI	GAAGAC	1 cut(s)	227
Bpu1102I	GCTNAGC	1 cut(s)	585
BpuMI	CCSGG	1 cut(s)	178
Bsc4I	CCNNNNNNNGG	1 cut(s)	768
Bse1I	ACTGG	4 cut(s)	314, 476, 511, 622
Bse3DI	GCAATG	1 cut(s)	336
BseLI	CCNNNNNNNGG	1 cut(s)	768
BseMI	GCAATG	1 cut(s)	336
BseMII	CTCAG	2 cut(s)	282, 894
BseNI	ACTGG	4 cut(s)	314, 476, 511, 622
BseRI	GAGGAG	1 cut(s)	1016
BseXI	GCAGC	4 cut(s)	313, 427, 539, 542
Bsh1236I	CGCG	1 cut(s)	232
BshFI	GGCC	1 cut(s)	692
BsiHKAI	GWGCWC	1 cut(s)	615
BsiHKCI	CYCGRG	2 cut(s)	614, 826
BsiSI	CCGG	1 cut(s)	178
BslFI	GGGAC	1 cut(s)	924
BslI	CCNNNNNNNGG	1 cut(s)	768
BsmAI	GTCTC	7 cut(s)	80, 479, 641, 822, 829, 949, 1007
BsmBI	CGTCTC	3 cut(s)	80, 822, 829
BsmFI	GGGAC	1 cut(s)	924
BsmI	GAATGC	2 cut(s)	261, 459
BsnI	GGCC	1 cut(s)	692
BsoBI	CYCGRG	2 cut(s)	614, 826
Bsp1286I	GDGCHC	1 cut(s)	615
Bsp143I	GATC	1 cut(s)	18
Bsp1720I	GCTNAGC	1 cut(s)	585
BspACI	CCGC	4 cut(s)	93, 230, 246, 443
BspANI	GGCC	1 cut(s)	692
BspCNI	CTCAG	2 cut(s)	281, 895
BspFNI	CGCG	1 cut(s)	232
BspHI	TCATGA	1 cut(s)	481
BspLI	GGNNCC	1 cut(s)	790
BspMAI	CTGCAG	1 cut(s)	133
BspQI	GCTCTTC	1 cut(s)	167
BsrDI	GCAATG	1 cut(s)	336
BsrI	ACTGG	4 cut(s)	314, 476, 511, 622
BssMI	GATC	1 cut(s)	18
Bst4CI	ACNGT	4 cut(s)	53, 348, 433, 523
Bst6I	CTCTTC	1 cut(s)	167
BstC8I	GCNNGC	1 cut(s)	133
BstDEI	CTNAG	3 cut(s)	268, 585, 903
BstFNI	CGCG	1 cut(s)	232
BstHHI	GCGC	1 cut(s)	183
BstKTI	GATC	1 cut(s)	21
BstMAI	GTCTC	7 cut(s)	80, 479, 641, 822, 829, 949, 1007
BstMBI	GATC	1 cut(s)	18
BstMWI	GCNNNNNNNGC	1 cut(s)	359
BstSCI	CCNGG	1 cut(s)	176
BstSFI	CTRYAG	3 cut(s)	129, 321, 522
BstUI	CGCG	1 cut(s)	232
BstV1I	GCAGC	4 cut(s)	313, 427, 539, 542
BstV2I	GAAGAC	1 cut(s)	227
BsuRI	GGCC	1 cut(s)	692
BtgZI	GCGATG	1 cut(s)	658
BtsIMutI	CAGTG	1 cut(s)	849
Cac8I	GCNNGC	1 cut(s)	133
CaiI	CAGNNNCTG	1 cut(s)	533
CciI	TCATGA	1 cut(s)	481
CfoI	GCGC	1 cut(s)	183
Cfr13I	GGNCC	1 cut(s)	59
CseI	GACGC	3 cut(s)	62, 221, 646
Csp6I	GTAC	2 cut(s)	207, 499
CviAII	CATG	4 cut(s)	379, 482, 595, 887
CviQI	GTAC	2 cut(s)	207, 499
DdeI	CTNAG	3 cut(s)	268, 585, 903
DpnI	GATC	1 cut(s)	20
DpnII	GATC	1 cut(s)	18
Eam1104I	CTCTTC	1 cut(s)	167
EarI	CTCTTC	1 cut(s)	167
Eco147I	AGGCCT	1 cut(s)	692
Eco32I	GATATC	1 cut(s)	840
Eco47I	GGWCC	1 cut(s)	59
Eco88I	CYCGRG	2 cut(s)	614, 826
EcoO109I	RGGNCCY	1 cut(s)	59
EcoRI	GAATTC	1 cut(s)	990
EcoRV	GATATC	1 cut(s)	840
EcoT22I	ATGCAT	2 cut(s)	295, 459
Esp3I	CGTCTC	3 cut(s)	80, 822, 829
FaeI	CATG	4 cut(s)	382, 485, 598, 890
FaqI	GGGAC	1 cut(s)	924
FatI	CATG	4 cut(s)	378, 481, 594, 886
FauNDI	CATATG	2 cut(s)	295, 767
FbaI	TGATCA	1 cut(s)	18
Fnu4HI	GCNGC	5 cut(s)	246, 327, 441, 528, 531
Fsp4HI	GCNGC	5 cut(s)	246, 327, 441, 528, 531
FspBI	CTAG	1 cut(s)	38
GlaI	GCGC	1 cut(s)	182
GluI	GCNGC	5 cut(s)	246, 327, 441, 528, 531
HaeIII	GGCC	1 cut(s)	692
HapII	CCGG	1 cut(s)	178
HgaI	GACGC	3 cut(s)	62, 221, 646
HhaI	GCGC	1 cut(s)	183
Hin1II	CATG	4 cut(s)	382, 485, 598, 890
Hin6I	GCGC	1 cut(s)	181
HinP1I	GCGC	1 cut(s)	181
HincII	GTYRAC	1 cut(s)	429
HindII	GTYRAC	1 cut(s)	429
HindIII	AAGCTT	1 cut(s)	719
HinfI	GANTC	2 cut(s)	487, 1006
HpaII	CCGG	1 cut(s)	178
HphI	GGTGA	4 cut(s)	16, 212, 692, 971
Hpy166II	GTNNAC	2 cut(s)	396, 429
Hpy188III	TCNNGA	5 cut(s)	122, 482, 514, 1010, 1035
Hpy8I	GTNNAC	2 cut(s)	396, 429
HpyAV	CCTTC	1 cut(s)	901
HpyCH4III	ACNGT	4 cut(s)	53, 348, 433, 523
HpyCH4IV	ACGT	1 cut(s)	116
HpyCH4V	TGCA	4 cut(s)	30, 131, 293, 457
HpyF10VI	GCNNNNNNNGC	1 cut(s)	359
HpyF3I	CTNAG	3 cut(s)	268, 585, 903
HpySE526I	ACGT	1 cut(s)	116
Hsp92II	CATG	4 cut(s)	382, 485, 598, 890
HspAI	GCGC	1 cut(s)	181
Ksp22I	TGATCA	1 cut(s)	18
Kzo9I	GATC	1 cut(s)	18
LguI	GCTCTTC	1 cut(s)	167
LmnI	GCTCC	1 cut(s)	788
Lsp1109I	GCAGC	4 cut(s)	313, 427, 539, 542
MaeI	CTAG	1 cut(s)	38
MaeII	ACGT	1 cut(s)	116
MalI	GATC	1 cut(s)	20
MboI	GATC	1 cut(s)	18
MboII	GAAGA	9 cut(s)	139, 154, 227, 302, 358, 367, 772, 796, 1017
MhlI	GDGCHC	1 cut(s)	615
MluCI	AATT	3 cut(s)	33, 855, 990
MlyI	GAGTC	2 cut(s)	481, 1015
MnlI	CCTC	6 cut(s)	50, 165, 721, 856, 970, 994
Mph1103I	ATGCAT	2 cut(s)	295, 459
MroXI	GAANNNNTTC	1 cut(s)	894
MseI	TTAA	3 cut(s)	384, 800, 1054
MslI	CAYNNNNRTG	1 cut(s)	665
MspA1I	CMGCKG	3 cut(s)	248, 326, 533
MspI	CCGG	1 cut(s)	178
MspR9I	CCNGG	1 cut(s)	178
Mva1269I	GAATGC	2 cut(s)	261, 459
MvnI	CGCG	1 cut(s)	232
MwoI	GCNNNNNNNGC	1 cut(s)	359
NciI	CCSGG	1 cut(s)	178
NdeI	CATATG	2 cut(s)	295, 767
NdeII	GATC	1 cut(s)	18
NlaIII	CATG	4 cut(s)	382, 485, 598, 890
NlaIV	GGNNCC	1 cut(s)	790
NsiI	ATGCAT	2 cut(s)	295, 459
PaeR7I	CTCGAG	1 cut(s)	614
PagI	TCATGA	1 cut(s)	481
PceI	AGGCCT	1 cut(s)	692
PciSI	GCTCTTC	1 cut(s)	167
PctI	GAATGC	2 cut(s)	261, 459
PdmI	GAANNNNTTC	1 cut(s)	894
PflMI	CCANNNNNTGG	1 cut(s)	768
PkrI	GCNGC	5 cut(s)	247, 328, 442, 529, 532
PleI	GAGTC	2 cut(s)	481, 1014
PpsI	GAGTC	2 cut(s)	481, 1014
PpuMI	RGGWCCY	1 cut(s)	59
PshBI	ATTAAT	1 cut(s)	800
Psp5II	RGGWCCY	1 cut(s)	59
PspN4I	GGNNCC	1 cut(s)	790
PspPI	GGNCC	1 cut(s)	59
PspPPI	RGGWCCY	1 cut(s)	59
PspXI	VCTCGAGB	1 cut(s)	614
PstI	CTGCAG	1 cut(s)	133
PstNI	CAGNNNCTG	1 cut(s)	533
PvuII	CAGCTG	2 cut(s)	326, 533
RsaI	GTAC	2 cut(s)	208, 500
RsaNI	GTAC	2 cut(s)	207, 499
RseI	CAYNNNNRTG	1 cut(s)	665
SapI	GCTCTTC	1 cut(s)	167
SaqAI	TTAA	3 cut(s)	384, 800, 1054
SatI	GCNGC	5 cut(s)	246, 327, 441, 528, 531
Sau3AI	GATC	1 cut(s)	18
Sau96I	GGNCC	1 cut(s)	59
SchI	GAGTC	2 cut(s)	481, 1015
ScrFI	CCNGG	1 cut(s)	178
SduI	GDGCHC	1 cut(s)	615
SfcI	CTRYAG	3 cut(s)	129, 321, 522
Sfr274I	CTCGAG	1 cut(s)	614
SinI	GGWCC	1 cut(s)	59
SlaI	CTCGAG	1 cut(s)	614
SmiMI	CAYNNNNRTG	1 cut(s)	665
SmlI	CTYRAG	1 cut(s)	614
SmoI	CTYRAG	1 cut(s)	614
Sse9I	AATT	3 cut(s)	33, 855, 990
SseBI	AGGCCT	1 cut(s)	692
SsiI	CCGC	4 cut(s)	93, 230, 246, 443
SspMI	CTAG	1 cut(s)	38
StuI	AGGCCT	1 cut(s)	692
StyD4I	CCNGG	1 cut(s)	176
TaaI	ACNGT	4 cut(s)	53, 348, 433, 523
TaiI	ACGT	1 cut(s)	119
TaqI	TCGA	1 cut(s)	615
TasI	AATT	3 cut(s)	33, 855, 990
TatI	WGTACW	2 cut(s)	206, 498
TauI	GCSGC	1 cut(s)	248
Tru1I	TTAA	3 cut(s)	384, 800, 1054
Tru9I	TTAA	3 cut(s)	384, 800, 1054
TscAI	CASTG	1 cut(s)	849
TseI	GCWGC	4 cut(s)	326, 440, 527, 530
TspDTI	ATGAA	6 cut(s)	139, 367, 470, 538, 661, 903
TspRI	CASTG	1 cut(s)	849
Van91I	CCANNNNNTGG	1 cut(s)	768
VpaK11BI	GGWCC	1 cut(s)	59
VspI	ATTAAT	1 cut(s)	800
XapI	RAATTY	1 cut(s)	990
XhoI	CTCGAG	1 cut(s)	614
XmnI	GAANNNNTTC	1 cut(s)	894
XspI	CTAG	1 cut(s)	38
Zsp2I	ATGCAT	2 cut(s)	295, 459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.