Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005715

Physical Location & Seq

Genomic Coordinates Forward (+)

29523497 .. 29527008

Gene Structure 3512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1135600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGAAACCGATCATGTTGAAAAACGTAACGCTGCCATAAAAAAGCTCCTTCTCGTTATAAGCTGCATCCTTTTATCCATAGGCACAGCTGGAGGTCCATTGGTTATGCGCCTCTACTTCATCCATGGAGGCAAACGCGTCTGGCTTTCGAGCTGGCTCGAAACCGGCGGCTGGCCGATCACCTTCATCCCCTTAGTCATGGCTTATTACCACCGCCGCAAGACTGAAGGCCCATCAACAAAAATCATCTTCATGAAGCTCCCACTCTTTATAGCCTCTGCCGTCATCGGCGTCCTCACCGGCCTGGATGACTACCTTTACGCCTACGGCGTGGCCCGACTTCCGGTCTCCACGTCAGCCCTAATCATCGCTGGCCAGCTAGCCTTCACTGCCCTTTTTGCCTTTATTCTTGTGAAGCAGAAGTTCACCTCATTCTCCATAAACGCCGTCGTGCTGTTGACTATTGGCGCGGCTGTTTTGGGGTTGAACACCAGCTCTGATCGACCAGAGGGGGAATCGAATAAGGAGTATATTGCCGGGTTTTTTATGACGGTGGCGGCGGCTGCTTTGTATGGATTTGTGCTGCCGTTGATAGAGTTGACATACAAGAAGGCCAAGCAGACCATTACATATGCTTTGGTTCTGGAGATTCAATTGGTTATGTGTTTGTTTGCTACTCTTTTTTGCACCGTGGGAATGTTTATCGACAACGACTTCAAGGTTATTCCAAGAGAAGCAAGAAACTTCGGTCTGGGGGAAACCAGTTACTATGTGGTGCTGGTATTTAGTGCTATACTGTGGCAAGGTTTTTTTCTGGGAGCCGTAGGAGTCATCTTCTGCGCCTCATCTTTGTTTTCTGGAATTCTAATTGCCGTTTTGCTCCCCGTAACCGAGGTCCTAGCAGTTATTTTTTACCATGAAAAATTCCAAGCAGTAAAGGGAGTCTCTCTTGCACTCTCTCTCTGGGGATTTGTCTCTTACTTCTATGGTGAGATAAAACACAGCAAGCAAGAGGAAAAGGGAAAGCAAGCCGAAATTTCAGAGAAAAAGAAAGAAACACCAGAAGTAACACAGGTTCCTCAACAACTGGAACCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005337 GO:0005345 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006863 GO:0008150 GO:0009914 GO:0010184 GO:0010817 GO:0015205 GO:0015211 GO:0015851 GO:0015858 GO:0015860 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071944 GO:0072530 GO:1901264 GO:1901505 GO:1901642 GO:1904823

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUNUT

Protein Analysis

366

Amino Acids

40.28

Weight (kDa)

8.83

Isoelectric Point (pI)

36.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUNUT	PF16913	17 - 336	9.1e-121	Purine nucleobase transmembrane transport

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005715)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28220 AT1G28230
fragaria_vesca	FvH4_5g36040 FvH4_5g36060
malus_domestica	MD00G1135600.v1.1 MD15G1379500.v1.1
prunus_persica	Prupe.1G525600_v2.0.a1 Prupe.1G525800_v2.0.a1
pyrus_communis	pycom08g16430 pycom15g33980 pycom15g34010
rosa_chinensis	RchiOBHm_Chr7g0237771 RchiOBHm_Chr7g0237851
rosa_laevigata	RLG00000000962 RLG00000000968
rosa_multiflora	Rmu_ssc0000067.1_g000004 Rmu_ssc0000067.1_g000031
rosa_roxburghii	Rroxscaffold_3G00223710 Rroxscaffold_3G00223760
rosa_rugosa	Rorug07G0308600
rosa_samantha	Rh7AG464000 Rh7BG434100 Rh7CG481000 Rh7CG481500 Rh7DG449900 Rh7DG450200
rosa_wichuraiana	Rw7G038390 Rw7G038400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	59
AccII	CGCG	2 cut(s)	138, 470
AciI	CCGC	6 cut(s)	168, 214, 217, 470, 557, 560
AcoI	YGGCCR	2 cut(s)	173, 373
AcsI	RAATTY	3 cut(s)	861, 923, 1035
AcuI	CTGAAG	1 cut(s)	246
AcyI	GRCGYC	1 cut(s)	291
AfiI	CCNNNNNNNGG	2 cut(s)	171, 343
AflIII	ACRYGT	1 cut(s)	136
AgsI	TTSAA	4 cut(s)	20, 487, 653, 718
AhdI	GACNNNNNGTC	1 cut(s)	344
AjiI	CACGTC	1 cut(s)	354
AjnI	CCWGG	1 cut(s)	303
AluBI	AGCT	7 cut(s)	46, 63, 89, 153, 259, 379, 495
AluI	AGCT	7 cut(s)	46, 63, 89, 153, 259, 379, 495
Alw26I	GTCTC	3 cut(s)	352, 949, 979
AoxI	GGCC	6 cut(s)	173, 229, 301, 333, 373, 612
ApeKI	GCWGC	4 cut(s)	32, 63, 563, 583
ApoI	RAATTY	3 cut(s)	861, 923, 1035
AspLEI	GCGC	3 cut(s)	111, 470, 842
AspS9I	GGNCC	4 cut(s)	95, 230, 334, 895
AsuC2I	CCSGG	1 cut(s)	538
AsuHPI	GGTGA	4 cut(s)	172, 289, 418, 1001
AsuNHI	GCTAGC	1 cut(s)	379
AvaII	GGWCC	2 cut(s)	95, 895
BalI	TGGCCA	1 cut(s)	375
BbvI	GCAGC	4 cut(s)	19, 50, 550, 570
BccI	CCATC	1 cut(s)	241
BceAI	ACGGC	6 cut(s)	266, 343, 431, 571, 806, 857
BciT130I	CCWGG	1 cut(s)	305
BcnI	CCSGG	1 cut(s)	538
BcoDI	GTCTC	3 cut(s)	352, 949, 979
BfaI	CTAG	3 cut(s)	380, 899, 1096
BisI	GCNGC	9 cut(s)	33, 64, 169, 217, 471, 558, 561, 564, 584
BlsI	GCNGC	9 cut(s)	34, 65, 170, 218, 472, 559, 562, 565, 585
Bme1390I	CCNGG	2 cut(s)	305, 538
Bme18I	GGWCC	2 cut(s)	95, 895
BmeRI	GACNNNNNGTC	1 cut(s)	344
BmgBI	CACGTC	1 cut(s)	354
BmgT120I	GGNCC	4 cut(s)	95, 230, 334, 895
BmiI	GGNNCC	3 cut(s)	820, 1077, 1092
BmrFI	CCNGG	2 cut(s)	305, 538
BmsI	GCATC	1 cut(s)	75
BmtI	GCTAGC	1 cut(s)	383
BpmI	CTGGAG	2 cut(s)	111, 665
BpuMI	CCSGG	1 cut(s)	538
BsaHI	GRCGYC	1 cut(s)	291
BsaI	GGTCTC	1 cut(s)	352
BsaJI	CCNNGG	3 cut(s)	124, 690, 891
BsaWI	WCCGGW	1 cut(s)	343
Bsc4I	CCNNNNNNNGG	2 cut(s)	171, 343
Bse118I	RCCGGY	2 cut(s)	164, 299
Bse1I	ACTGG	2 cut(s)	762, 1092
BseBI	CCWGG	1 cut(s)	305
BseDI	CCNNGG	3 cut(s)	124, 690, 891
BseGI	GGATG	4 cut(s)	66, 120, 186, 313
BseLI	CCNNNNNNNGG	2 cut(s)	171, 343
BseNI	ACTGG	2 cut(s)	762, 1092
BseXI	GCAGC	4 cut(s)	19, 50, 550, 570
Bsh1236I	CGCG	2 cut(s)	138, 470
BshFI	GGCC	6 cut(s)	175, 231, 303, 335, 375, 614
BsiSI	CCGG	4 cut(s)	165, 300, 344, 537
BslI	CCNNNNNNNGG	2 cut(s)	171, 343
BsmAI	GTCTC	3 cut(s)	352, 949, 979
BsnI	GGCC	6 cut(s)	175, 231, 303, 335, 375, 614
Bso31I	GGTCTC	1 cut(s)	352
Bsp143I	GATC	3 cut(s)	10, 177, 499
Bsp19I	CCATGG	1 cut(s)	124
BspACI	CCGC	6 cut(s)	168, 214, 217, 470, 557, 560
BspANI	GGCC	6 cut(s)	175, 231, 303, 335, 375, 614
BspFNI	CGCG	2 cut(s)	138, 470
BspHI	TCATGA	1 cut(s)	252
BspLI	GGNNCC	3 cut(s)	820, 1077, 1092
BspOI	GCTAGC	1 cut(s)	383
BspTNI	GGTCTC	1 cut(s)	352
BsrFI	RCCGGY	2 cut(s)	164, 299
BsrI	ACTGG	2 cut(s)	762, 1092
BssAI	RCCGGY	2 cut(s)	164, 299
BssECI	CCNNGG	3 cut(s)	124, 690, 891
BssMI	GATC	3 cut(s)	10, 177, 499
BssNI	GRCGYC	1 cut(s)	291
BssT1I	CCWWGG	1 cut(s)	124
Bst2UI	CCWGG	1 cut(s)	305
Bst4CI	ACNGT	3 cut(s)	553, 691, 798
BstACI	GRCGYC	1 cut(s)	291
BstC8I	GCNNGC	7 cut(s)	155, 173, 373, 377, 381, 1007, 1029
BstDEI	CTNAG	1 cut(s)	193
BstDSI	CCRYGG	2 cut(s)	124, 690
BstF5I	GGATG	4 cut(s)	66, 120, 186, 313
BstFNI	CGCG	2 cut(s)	138, 470
BstHHI	GCGC	3 cut(s)	111, 470, 842
BstKTI	GATC	3 cut(s)	13, 180, 502
BstMAI	GTCTC	3 cut(s)	352, 949, 979
BstMBI	GATC	3 cut(s)	10, 177, 499
BstMWI	GCNNNNNNNGC	3 cut(s)	389, 398, 563
BstNI	CCWGG	1 cut(s)	305
BstSCI	CCNGG	2 cut(s)	303, 536
BstUI	CGCG	2 cut(s)	138, 470
BstV1I	GCAGC	4 cut(s)	19, 50, 550, 570
BsuRI	GGCC	6 cut(s)	175, 231, 303, 335, 375, 614
BtgI	CCRYGG	2 cut(s)	124, 690
BtgZI	GCGATG	1 cut(s)	352
BtrI	CACGTC	1 cut(s)	354
BtsCI	GGATG	4 cut(s)	66, 120, 186, 313
BtsI	GCAGTG	1 cut(s)	387
BtsIMutI	CAGTG	1 cut(s)	387
Cac8I	GCNNGC	7 cut(s)	155, 173, 373, 377, 381, 1007, 1029
CciI	TCATGA	1 cut(s)	252
CfoI	GCGC	3 cut(s)	111, 470, 842
Cfr10I	RCCGGY	2 cut(s)	164, 299
Cfr13I	GGNCC	4 cut(s)	95, 230, 334, 895
CseI	GACGC	2 cut(s)	127, 280
CviAII	CATG	5 cut(s)	14, 125, 199, 253, 917
DdeI	CTNAG	1 cut(s)	193
DpnI	GATC	3 cut(s)	12, 179, 501
DpnII	GATC	3 cut(s)	10, 177, 499
DriI	GACNNNNNGTC	1 cut(s)	344
EaeI	YGGCCR	2 cut(s)	173, 373
Eam1105I	GACNNNNNGTC	1 cut(s)	344
Eco130I	CCWWGG	1 cut(s)	124
Eco31I	GGTCTC	1 cut(s)	352
Eco47I	GGWCC	2 cut(s)	95, 895
Eco57I	CTGAAG	1 cut(s)	246
EcoO109I	RGGNCCY	1 cut(s)	895
EcoRI	GAATTC	1 cut(s)	861
EcoRII	CCWGG	1 cut(s)	303
EcoT14I	CCWWGG	1 cut(s)	124
ErhI	CCWWGG	1 cut(s)	124
FaeI	CATG	5 cut(s)	17, 128, 202, 256, 920
FatI	CATG	5 cut(s)	13, 124, 198, 252, 916
FauNDI	CATATG	1 cut(s)	631
Fnu4HI	GCNGC	9 cut(s)	33, 64, 169, 217, 471, 558, 561, 564, 584
FokI	GGATG	4 cut(s)	53, 107, 173, 320
Fsp4HI	GCNGC	9 cut(s)	33, 64, 169, 217, 471, 558, 561, 564, 584
FspBI	CTAG	3 cut(s)	380, 899, 1096
GlaI	GCGC	3 cut(s)	110, 469, 841
GluI	GCNGC	9 cut(s)	33, 64, 169, 217, 471, 558, 561, 564, 584
GsuI	CTGGAG	2 cut(s)	111, 665
HaeIII	GGCC	6 cut(s)	175, 231, 303, 335, 375, 614
HapII	CCGG	4 cut(s)	165, 300, 344, 537
HgaI	GACGC	2 cut(s)	127, 280
HhaI	GCGC	3 cut(s)	111, 470, 842
Hin1I	GRCGYC	1 cut(s)	291
Hin1II	CATG	5 cut(s)	17, 128, 202, 256, 920
Hin6I	GCGC	3 cut(s)	109, 468, 840
HinP1I	GCGC	3 cut(s)	109, 468, 840
HincII	GTYRAC	2 cut(s)	459, 600
HindII	GTYRAC	2 cut(s)	459, 600
HinfI	GANTC	4 cut(s)	515, 649, 828, 942
HpaII	CCGG	4 cut(s)	165, 300, 344, 537
HphI	GGTGA	4 cut(s)	172, 289, 418, 1001
Hpy166II	GTNNAC	3 cut(s)	426, 459, 600
Hpy188I	TCNGA	2 cut(s)	499, 1042
Hpy188III	TCNNGA	3 cut(s)	253, 644, 858
Hpy8I	GTNNAC	3 cut(s)	426, 459, 600
Hpy99I	CGWCG	1 cut(s)	452
HpyAV	CCTTC	5 cut(s)	59, 193, 221, 394, 604
HpyCH4III	ACNGT	3 cut(s)	553, 691, 798
HpyCH4IV	ACGT	2 cut(s)	25, 353
HpyCH4V	TGCA	3 cut(s)	66, 687, 953
HpyF10VI	GCNNNNNNNGC	3 cut(s)	389, 398, 563
HpyF3I	CTNAG	1 cut(s)	193
HpySE526I	ACGT	2 cut(s)	25, 353
Hsp92I	GRCGYC	1 cut(s)	291
Hsp92II	CATG	5 cut(s)	17, 128, 202, 256, 920
HspAI	GCGC	3 cut(s)	109, 468, 840
Kzo9I	GATC	3 cut(s)	10, 177, 499
LmnI	GCTCC	4 cut(s)	51, 264, 818, 885
Lsp1109I	GCAGC	4 cut(s)	19, 50, 550, 570
LweI	GCATC	1 cut(s)	75
MaeI	CTAG	3 cut(s)	380, 899, 1096
MaeII	ACGT	2 cut(s)	25, 353
MaeIII	GTNAC	4 cut(s)	26, 764, 886, 1066
MalI	GATC	3 cut(s)	12, 179, 501
MboI	GATC	3 cut(s)	10, 177, 499
MboII	GAAGA	2 cut(s)	241, 826
MfeI	CAATTG	1 cut(s)	653
MlsI	TGGCCA	1 cut(s)	375
MluCI	AATT	5 cut(s)	653, 861, 867, 923, 1035
MluI	ACGCGT	1 cut(s)	136
MluNI	TGGCCA	1 cut(s)	375
MlyI	GAGTC	2 cut(s)	837, 951
Mox20I	TGGCCA	1 cut(s)	375
MscI	TGGCCA	1 cut(s)	375
MslI	CAYNNNNRTG	1 cut(s)	251
Msp20I	TGGCCA	1 cut(s)	375
MspA1I	CMGCKG	1 cut(s)	89
MspI	CCGG	4 cut(s)	165, 300, 344, 537
MspR9I	CCNGG	2 cut(s)	305, 538
MunI	CAATTG	1 cut(s)	653
MvaI	CCWGG	1 cut(s)	305
MvnI	CGCG	2 cut(s)	138, 470
MwoI	GCNNNNNNNGC	3 cut(s)	389, 398, 563
NciI	CCSGG	1 cut(s)	538
NcoI	CCATGG	1 cut(s)	124
NdeI	CATATG	1 cut(s)	631
NdeII	GATC	3 cut(s)	10, 177, 499
NheI	GCTAGC	1 cut(s)	379
NlaIII	CATG	5 cut(s)	17, 128, 202, 256, 920
NlaIV	GGNNCC	3 cut(s)	820, 1077, 1092
PagI	TCATGA	1 cut(s)	252
PcsI	WCGNNNNNNNCGW	1 cut(s)	327
PfeI	GAWTC	2 cut(s)	515, 649
PkrI	GCNGC	9 cut(s)	34, 65, 170, 218, 472, 559, 562, 565, 585
PleI	GAGTC	2 cut(s)	836, 950
PpsI	GAGTC	2 cut(s)	836, 950
PpuMI	RGGWCCY	1 cut(s)	895
PsiI	TTATAA	1 cut(s)	59
Psp5II	RGGWCCY	1 cut(s)	895
Psp6I	CCWGG	1 cut(s)	303
PspGI	CCWGG	1 cut(s)	303
PspN4I	GGNNCC	3 cut(s)	820, 1077, 1092
PspPI	GGNCC	4 cut(s)	95, 230, 334, 895
PspPPI	RGGWCCY	1 cut(s)	895
PsrI	GAACNNNNNNTAC	2 cut(s)	1059, 1091
PvuII	CAGCTG	1 cut(s)	89
RseI	CAYNNNNRTG	1 cut(s)	251
SatI	GCNGC	9 cut(s)	33, 64, 169, 217, 471, 558, 561, 564, 584
Sau3AI	GATC	3 cut(s)	10, 177, 499
Sau96I	GGNCC	4 cut(s)	95, 230, 334, 895
SchI	GAGTC	2 cut(s)	837, 951
ScrFI	CCNGG	2 cut(s)	305, 538
SfaNI	GCATC	1 cut(s)	75
SinI	GGWCC	2 cut(s)	95, 895
SmiMI	CAYNNNNRTG	1 cut(s)	251
Sse9I	AATT	5 cut(s)	653, 861, 867, 923, 1035
SsiI	CCGC	6 cut(s)	168, 214, 217, 470, 557, 560
SspMI	CTAG	3 cut(s)	380, 899, 1096
StyD4I	CCNGG	2 cut(s)	303, 536
StyI	CCWWGG	1 cut(s)	124
TaaI	ACNGT	3 cut(s)	553, 691, 798
TaiI	ACGT	2 cut(s)	28, 356
TaqI	TCGA	5 cut(s)	149, 159, 502, 518, 705
TaqII	GACCGA	1 cut(s)	737
TasI	AATT	5 cut(s)	653, 861, 867, 923, 1035
TauI	GCSGC	5 cut(s)	171, 219, 473, 560, 563
TfiI	GAWTC	2 cut(s)	515, 649
TscAI	CASTG	1 cut(s)	394
TseI	GCWGC	4 cut(s)	32, 63, 563, 583
TspDTI	ATGAA	5 cut(s)	109, 175, 241, 269, 933
TspRI	CASTG	1 cut(s)	394
VpaK11BI	GGWCC	2 cut(s)	95, 895
XapI	RAATTY	3 cut(s)	861, 923, 1035
XcmI	CCANNNNNNNNNTGG	1 cut(s)	769
XspI	CTAG	3 cut(s)	380, 899, 1096

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.