Rh7BG434100

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005715
Physical Location & Seq
Reverse (-)
49781184 .. 49782851
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG434100.1

Sequence Viewer

Length: 1047 bp
ATGGAAACCACTGAGGATGAGCAACGTAAAGCCGCCGCCCTGAAAAGGTTGCTGCTCATTCTGAGCTGCGTATTCTTATCCATAGGCACCTGTGGCGGCCCTTTGATAATGCGCCTCTACTTCATCCACGGCGGCAAGCGCGTCTGGCTTTCGAGCATGCTCGAAACCGGTGGCTGGCCCATAATCCTCGTCCCTATAGCCATAGCCTACTACCACCGCCGCAAAAACCAAGCTCCAACTGAACCACCAACCAAGCTCTTCTTCATGAAGCTCCCTCTCTTCGTTGCCTCCGCCTTCATCGGCGTCCTCACCGGCCTCGACGACTACCTGTACGCCTACGGTGTCGCACGCCTCCCCGTCTCCACCTCAGCCCTCATCATAGCGGCTCAGCTGGCTTTCACGGCCTTTTTTGCCTTCATACTTGTGAAGCAGAAGTTCACGTCGTATTCCACAAATGCCATCGTGTTGCTGACGGTGGGGGCGGCGGTTCTGGGCCTGAACACGAGCTCGGACCGGCCGGAGGGGGAGTCGAATGCGCAGTACATGCTCGGGTTTATGATGACGGTGGCCGCGGCGGCGTTGTATGGGTTTGTGTTGCCGTTGGTGGAGTTGATGTACAAGAAAGCCAAGCAGAACATCACGTATGCTTTGGTTTTGGAGATTCAGTTGGTTATGTGTTTGTTTGCTACCATTTTCTGCACTGTTGGAATGCTGGTCAACAATGATTTCAAGGTAATTCCAAGAGAAGCAAGGAAATTCGAGCTTGGGGAGACTACCTACTATGTGTTGCTTGTATCGTCTCTGCTATCCGGCATCGTAATCGCTGTTCTGCTCCCGATAACAGAGATCTTTGCTGTGATTTTCTACCACGAAAAGTTCCAAGCAGAAAAGGGCGTTTCTCTTGTGCTCTCCCTCTGGGGATTTGTCTCATACTTCTACGGCGAAATCAAACACAACAAGGAAAGCAAGCGAGTTAAGAAAAAGGAAAACAACAAGGACAGTAGTGGCCAAGACACAGAAAACCCTCAGTCAGTTCCAAATCCATGA

Protein Analysis

348

Amino Acids

38.67

Weight (kDa)

9.06

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 18 - 265 4.3e-84 Purine nucleobase transmembrane transport
EamA PF00892 63 - 164 2.9e-08 EamA-like transporter family
Nuc_sug_transp PF04142 111 - 277 5.4e-07 Nucleotide-sugar transporter
PUNUT PF16913 262 - 320 1.8e-20 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 86
AccII CGCG 2 cut(s) 141, 572
AcoI YGGCCR 3 cut(s) 515, 567, 1006
AcsI RAATTY 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 3 cut(s) 332, 542, 617
AfiI CCNNNNNNNGG 3 cut(s) 45, 174, 520
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 1 cut(s) 730
AjiI CACGTC 1 cut(s) 441
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 7 cut(s) 66, 233, 256, 271, 391, 507, 763
AluI AGCT 7 cut(s) 66, 233, 256, 271, 391, 507, 763
Alw21I GWGCWC 2 cut(s) 509, 909
Alw26I GTCTC 4 cut(s) 364, 764, 804, 931
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 8 cut(s) 97, 176, 313, 402, 493, 515, 567, 1006
ApeKI GCWGC 2 cut(s) 52, 66
ApoI RAATTY 1 cut(s) 755
AsiGI ACCGGT 1 cut(s) 167
AspLEI GCGC 3 cut(s) 114, 141, 538
AspS9I GGNCC 4 cut(s) 98, 177, 493, 511
AsuHPI GGTGA 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 511
BalI TGGCCA 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 1 cut(s) 509
BauI CACGAG 1 cut(s) 502
Bbv12I GWGCWC 2 cut(s) 509, 909
BbvCI CCTCAGC 1 cut(s) 367
BbvI GCAGC 2 cut(s) 39, 53
BccI CCATC 1 cut(s) 467
BceAI ACGGC 4 cut(s) 145, 417, 583, 955
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BcoDI GTCTC 4 cut(s) 364, 764, 804, 931
BfmI CTRYAG 1 cut(s) 195
BglI GCCNNNNNGGC 1 cut(s) 575
BglII AGATCT 1 cut(s) 846
BlpI GCTNAGC 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 511
BmeT110I CYCGRG 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 4 cut(s) 98, 177, 493, 511
BmiI GGNNCC 1 cut(s) 88
BmsI GCATC 1 cut(s) 822
Bpu10I CCTNAGC 1 cut(s) 367
Bpu1102I GCTNAGC 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 642
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 127, 570
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 512, 542, 599, 629, 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 174, 520
Bse118I RCCGGY 3 cut(s) 167, 311, 513
BseDI CCNNGG 2 cut(s) 127, 570
BseGI GGATG 2 cut(s) 22, 123
BseLI CCNNNNNNNGG 3 cut(s) 45, 174, 520
BseMII CTCAG 4 cut(s) 53, 381, 401, 1040
BseX3I CGGCCG 1 cut(s) 515
BseXI GCAGC 2 cut(s) 39, 53
BsgI GTGCAG 1 cut(s) 682
Bsh1236I CGCG 2 cut(s) 141, 572
Bsh1285I CGRYCG 1 cut(s) 518
BshFI GGCC 8 cut(s) 99, 178, 315, 404, 495, 517, 569, 1008
BshNI GGYRCC 1 cut(s) 86
BshTI ACCGGT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 509, 909
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 5 cut(s) 168, 312, 514, 518, 810
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 45, 174, 520
BsmAI GTCTC 4 cut(s) 364, 764, 804, 931
BsmBI CGTCTC 2 cut(s) 364, 804
BsmFI GGGAC 1 cut(s) 176
BsmI GAATGC 2 cut(s) 538, 714
BsnI GGCC 8 cut(s) 99, 178, 315, 404, 495, 517, 569, 1008
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 509, 909
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 1 cut(s) 846
Bsp1720I GCTNAGC 1 cut(s) 387
BspANI GGCC 8 cut(s) 99, 178, 315, 404, 495, 517, 569, 1008
BspCNI CTCAG 5 cut(s) 4, 54, 380, 400, 1039
BspFNI CGCG 2 cut(s) 141, 572
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 88
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 86
BsrFI RCCGGY 3 cut(s) 167, 311, 513
BsrGI TGTACA 1 cut(s) 615
BssAI RCCGGY 3 cut(s) 167, 311, 513
BssECI CCNNGG 2 cut(s) 127, 570
BssMI GATC 1 cut(s) 846
BssNI GRCGYC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 502
Bst2BI CACGAG 1 cut(s) 502
Bst4CI ACNGT 5 cut(s) 341, 475, 565, 703, 1001
Bst6I CTCTTC 2 cut(s) 263, 284
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 544
BstAUI TGTACA 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 642
BstC8I GCNNGC 6 cut(s) 137, 158, 176, 349, 393, 968
BstDEI CTNAG 5 cut(s) 12, 62, 367, 387, 1026
BstDSI CCRYGG 2 cut(s) 127, 570
BstF5I GGATG 2 cut(s) 22, 123
BstFNI CGCG 2 cut(s) 141, 572
BstHHI GCGC 3 cut(s) 114, 141, 538
BstKTI GATC 1 cut(s) 849
BstMAI GTCTC 4 cut(s) 364, 764, 804, 931
BstMBI GATC 1 cut(s) 846
BstMCI CGRYCG 1 cut(s) 518
BstMWI GCNNNNNNNGC 8 cut(s) 93, 138, 145, 392, 401, 410, 544, 575
BstNSI RCATGY 2 cut(s) 160, 547
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 2 cut(s) 141, 572
BstV1I GCAGC 2 cut(s) 39, 53
BstX2I RGATCY 1 cut(s) 846
BstYI RGATCY 1 cut(s) 846
BstZI CGGCCG 1 cut(s) 515
BsuRI GGCC 8 cut(s) 99, 178, 315, 404, 495, 517, 569, 1008
BtgI CCRYGG 2 cut(s) 127, 570
BtrI CACGTC 1 cut(s) 441
BtsCI GGATG 2 cut(s) 22, 123
BtsIMutI CAGTG 2 cut(s) 9, 699
Cac8I GCNNGC 6 cut(s) 137, 158, 176, 349, 393, 968
CciI TCATGA 1 cut(s) 264
CfoI GCGC 3 cut(s) 114, 141, 538
Cfr10I RCCGGY 3 cut(s) 167, 311, 513
Cfr13I GGNCC 4 cut(s) 98, 177, 493, 511
Cfr42I CCGCGG 1 cut(s) 573
CpoI CGGWCCG 1 cut(s) 511
CseI GACGC 2 cut(s) 130, 292
Csp6I GTAC 3 cut(s) 331, 541, 616
CspAI ACCGGT 1 cut(s) 167
CspI CGGWCCG 1 cut(s) 511
CviAII CATG 4 cut(s) 157, 265, 544, 1044
CviQI GTAC 3 cut(s) 331, 541, 616
DdeI CTNAG 5 cut(s) 12, 62, 367, 387, 1026
DpnI GATC 1 cut(s) 848
DpnII GATC 1 cut(s) 846
EaeI YGGCCR 3 cut(s) 515, 567, 1006
EagI CGGCCG 1 cut(s) 515
Eam1104I CTCTTC 2 cut(s) 263, 284
EarI CTCTTC 2 cut(s) 263, 284
EciI GGCGGA 1 cut(s) 280
Ecl136II GAGCTC 1 cut(s) 507
EclXI CGGCCG 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 511
Eco52I CGGCCG 1 cut(s) 515
Eco53kI GAGCTC 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 509
Esp3I CGTCTC 2 cut(s) 364, 804
FaeI CATG 4 cut(s) 160, 268, 547, 1047
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 176
FatI CATG 4 cut(s) 156, 264, 543, 1043
FokI GGATG 2 cut(s) 29, 110
FriOI GRGCYC 1 cut(s) 509
FspI TGCGCA 1 cut(s) 537
GlaI GCGC 3 cut(s) 113, 140, 537
HaeIII GGCC 8 cut(s) 99, 178, 315, 404, 495, 517, 569, 1008
HapII CCGG 5 cut(s) 168, 312, 514, 518, 810
HgaI GACGC 2 cut(s) 130, 292
HhaI GCGC 3 cut(s) 114, 141, 538
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 4 cut(s) 160, 268, 547, 1047
Hin6I GCGC 3 cut(s) 112, 139, 536
HinP1I GCGC 3 cut(s) 112, 139, 536
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 2 cut(s) 527, 661
HpaII CCGG 5 cut(s) 168, 312, 514, 518, 810
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 438, 718
Hpy188I TCNGA 2 cut(s) 63, 511
Hpy188III TCNNGA 2 cut(s) 265, 835
Hpy8I GTNNAC 2 cut(s) 438, 718
Hpy99I CGWCG 2 cut(s) 323, 445
HpyAV CCTTC 2 cut(s) 304, 424
HpyCH4III ACNGT 5 cut(s) 341, 475, 565, 703, 1001
HpyCH4IV ACGT 3 cut(s) 25, 440, 641
HpyCH4V TGCA 1 cut(s) 699
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 138, 145, 392, 401, 410, 544, 575
HpyF3I CTNAG 5 cut(s) 12, 62, 367, 387, 1026
HpySE526I ACGT 3 cut(s) 25, 440, 641
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 4 cut(s) 160, 268, 547, 1047
HspAI GCGC 3 cut(s) 112, 139, 536
KspI CCGCGG 1 cut(s) 573
Kzo9I GATC 1 cut(s) 846
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 3 cut(s) 238, 276, 837
Lsp1109I GCAGC 2 cut(s) 39, 53
LweI GCATC 1 cut(s) 822
MaeII ACGT 3 cut(s) 25, 440, 641
MalI GATC 1 cut(s) 848
MboI GATC 1 cut(s) 846
MboII GAAGA 3 cut(s) 250, 253, 271
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 2 cut(s) 509, 909
MlsI TGGCCA 1 cut(s) 1008
MluCI AATT 2 cut(s) 735, 755
MluNI TGGCCA 1 cut(s) 1008
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 2 cut(s) 260, 685
Mox20I TGGCCA 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 1008
MseI TTAA 1 cut(s) 975
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 1008
MspA1I CMGCKG 2 cut(s) 391, 572
MspI CCGG 5 cut(s) 168, 312, 514, 518, 810
Mva1269I GAATGC 2 cut(s) 538, 714
MvnI CGCG 2 cut(s) 141, 572
MwoI GCNNNNNNNGC 8 cut(s) 93, 138, 145, 392, 401, 410, 544, 575
NdeII GATC 1 cut(s) 846
NlaIII CATG 4 cut(s) 160, 268, 547, 1047
NlaIV GGNNCC 1 cut(s) 88
NsbI TGCGCA 1 cut(s) 537
NspI RCATGY 2 cut(s) 160, 547
PaeI GCATGC 1 cut(s) 160
PagI TCATGA 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 2 cut(s) 538, 714
PfeI GAWTC 1 cut(s) 661
PinAI ACCGGT 1 cut(s) 167
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
Ppu21I YACGTR 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 4 cut(s) 98, 177, 493, 511
PsrI GAACNNNNNNTAC 2 cut(s) 810, 842
PsuI RGATCY 1 cut(s) 846
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 3 cut(s) 332, 542, 617
RsaNI GTAC 3 cut(s) 331, 541, 616
RseI CAYNNNNRTG 1 cut(s) 422
Rsr2I CGGWCCG 1 cut(s) 511
RsrII CGGWCCG 1 cut(s) 511
SacI GAGCTC 1 cut(s) 509
SacII CCGCGG 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 975
Sau3AI GATC 1 cut(s) 846
Sau96I GGNCC 4 cut(s) 98, 177, 493, 511
SchI GAGTC 1 cut(s) 536
SduI GDGCHC 2 cut(s) 509, 909
SfaNI GCATC 1 cut(s) 822
SfcI CTRYAG 1 cut(s) 195
Sfr303I CCGCGG 1 cut(s) 573
SgrBI CCGCGG 1 cut(s) 573
SinI GGWCC 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 422
SphI GCATGC 1 cut(s) 160
Sse9I AATT 2 cut(s) 735, 755
SstI GAGCTC 1 cut(s) 509
TaaI ACNGT 5 cut(s) 341, 475, 565, 703, 1001
TaiI ACGT 3 cut(s) 28, 443, 644
TaqI TCGA 5 cut(s) 152, 162, 318, 530, 759
TasI AATT 2 cut(s) 735, 755
TatI WGTACW 2 cut(s) 540, 615
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 1 cut(s) 975
Tru9I TTAA 1 cut(s) 975
TscAI CASTG 2 cut(s) 16, 706
TseI GCWGC 2 cut(s) 52, 66
TspDTI ATGAA 5 cut(s) 112, 253, 281, 286, 406
TspRI CASTG 2 cut(s) 16, 706
VpaK11BI GGWCC 1 cut(s) 511
XapI RAATTY 1 cut(s) 755
XceI RCATGY 2 cut(s) 160, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.