AT1G28260

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002793
Physical Location & Seq
Reverse (-)
9875527 .. 9879433
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28260.2

Sequence Viewer

Length: 2643 bp
ATGGAAGCTAATTCAGCTGATCAGAAACAAAAACCTAACTTTCTTGTTGAGGTTAATAACATTGAGAAACAGCTATGGACATTAATCCATTCAAAGACTATATTGCATACCGATGTTTCAGAATTATACGCGAAAGCTGGTTCGACCTACGAGCAAATCTTTAAAAGCAATCTCCAACACGAAGAGCTTCAAGAAGTAGAGTTCTGTCTCTGGAAACTCCACTATAAACACATCGATGAGTTTCGTAAAGGCCTTAAAACAAATGATCATGCCAAGCATATGAAAGCATTCAAGTTGTTTCTGTCAAAAGCAGCTGAGTTTTACCAGAATCTGATTTCCAAAGTCAGAGGATATTACCACAGACTCTCCGAGGAATCAGGAGAGCAAAAAAGCAGATTCTTATGCCATCGTTTCTACATTTGTCTTGGAGATCTTCAAAGGTATCAAGAACAATATCTGAAAGCACATGAACATCCTAATTGGTCAACTGCAGCTACTTACTATCTTGAAGCTGCAAAATCTTGGCCTGATAGTGGAAATCCTCATAACCAGCTAGCTGTATTGGCCACATATGTTAGTGATGAGTTATTGGCTCTGTATCACTGCGTAAGAAGCTTAGCTGTGAAAGAGCCTTTTCCTGGTGCTTCGAACAATCTTCTTTTGTTGTTTGAGAAGAACAGGTCATCTCCATTGCAGTCTCTGTCTACTGATGCAGAGTTTAACTACTTAAACCCATCAGAGAAAAAAGTTTCTGTGAAGGAACGAGATTTGTCAAAAGCAAAAGGAGAACTAGTTGCCGGAATTGATTTATGGCCCCTGGTTGTTCGAACAACTAGCTTCTTTTTCCTTAAATCCAGTTTTGATGAGTTTGGTCGGGCATTTGCATCAACTATAAGAGAACTGGATGCAGCGTTTGCAGCAGATGATAGAAATCTAGAAGCCATGTTGGAGTCTTATCAATTTATGGACACGGCGAGAAAGGGACCTTATAAAATCCTCCAAATTGTTGCGGTTTTTATCTACATCTTCCACAATTTAGCAGAGGCTAATGGATCCGACATCGTGAAAGAGGAAGTTAAGCTAACAAATTTAGCATTAACTATGGTGTTTATCGTCATGGGGCGAGTTGTTGAGAGATGTTTGAAGACAACTCCCTTGGATTCTTGCCCTCTCTTACCTGCTCTACTTGTATTTCTAGACTACTTACCGTTTCTGCTTGACAAGGTAGAAGAAGAAGAAGAAGAATGCAGGTTTGATGAGAAGAGCAAGAGTGCAATATCTTACTTCTTTGGCAAACTCGTTGATATCTTGAACCAGTTAAAGGTGAAAGACAAAAACTGCCCTGCTAAAACGTTGTTAGCTCTCTGGGAAGATCATGAACTGAAGTCCTTGGCTCCTCTGGCTCCCATACATGCACTTTTGGATTTCTCAAGTAATATGGATCTTAGAGAGAGTTTTGATAGAGGGAAGGAGCTTCGTCTTCAACGGATTATTAGTTCAGCAATTGATATCACAACTAGGCAGAAGAAGGGTTCTCAAAAGTGGCTATTCTTTGACAATCAACGGACTCATTTCTACACAACTTCGGGTGAATTGCAAAGCAACGGAGAGCTCTTTCATGGAAATGGAGAGGGTAGAAACAGAAAATGCGTTACTATTGGACCAGTTGAAATCATTCCTTTGGAGAATGAAAGATCTGTTCCTGTAGAAGAGGAAGAAGTCATTCTTCTCAAGCCGCTCGTAAGGTGTCAATCTGCTCCAATATATTCTTCTGGCATTGCAGCAAAGCCACTTAGCTCTGATTGCACAACTTCAGGAAATCAAACAACTACATCCAATGACAGCCTAAGACGTACCTTATCACTCATTGGGTCTGAAAGTTTCAGCTTTACGCAAGGTCTTAAGGACACGGATCCACAACATTTGCATCTCGAAGAAGGCACTGTCTCAGGAAGACCTCCATCGCTGAGCGCGTGGGTGGTTGACAAGAACAAAGAGAAAGGACGACTCGGTTTAAGTAAACCAAACGGATTGGGCCCTATTGATGAAACAGGTCCTGTCTCAGCCTTTGATAGTCTCTCCATCAATAGCAGCACCGAGCATCCTGCGTCTTCCTATTCGCCTCCTACTCCGTCTGCTCCACTACTGCCTGAAGACGCTTCTTGGTTTCATAATGACGCTAGTACTAACAAAGCAGAGAGCTTCTATGATCAGACACGATATATGGAACTCCCCGGTATCATGAAGCCATACACAAACCCACCCTTTGTTGGGATTTCTTCTTCTGAATGGTTACGTCGGTACAGAGAAAGTAGGAATCTTGGACCAGCCTATTCCTATCAAGCTCAGGGAACAAACAATTTGAGAAACTTTATGGCTCATGGCTCTTCCAAGTTCAGTCTCTTAGCTAGGTACGGGACACCAAACGATTCTTCTCAAAACTCAACGTTTCACCCTCAGCTCTACATGGAAGACCATGAATCTAGAGGAGAGAAGCTCGGCAACGTTCAACAAAGCACAACAAATCCTTATGGCTTCAGTGACGATCCCGGACCGTTTCTTAGATTTCTGAGAGAAAAAGAGTGGCTGAATGAGAACGGTCAGAGGCTAAGAGGACCTCCTCCTGCATATATGAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

880

Amino Acids

99.78

Weight (kDa)

6.13

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 66 - 152 2.3e-08 Telomerase activating protein Est1
EST1_DNA_bind PF10373 164 - 469 6.6e-57 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
Acc36I ACCTGC 2 cut(s) 1186, 1239
AccBSI CCGCTC 1 cut(s) 1738
AccI GTMKAC 1 cut(s) 704
AccII CGCG 2 cut(s) 131, 1973
AciI CCGC 2 cut(s) 1010, 1736
AclI AACGTT 3 cut(s) 1352, 2447, 2505
AclWI GGATC 6 cut(s) 1047, 1060, 1449, 1907, 1920, 2540
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 1 cut(s) 1087
AcuI CTGAAG 4 cut(s) 1403, 1797, 2172, 2521
AfaI GTAC 4 cut(s) 1855, 2185, 2303, 2414
AfiI CCNNNNNNNGG 5 cut(s) 533, 638, 1321, 2270, 2271
AflII CTTAAG 1 cut(s) 1901
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 2 cut(s) 637, 816
AjuI GAANNNNNNNTTGG 4 cut(s) 1753, 1785, 2416, 2448
AloI GAACNNNNNNTCC 2 cut(s) 1480, 1512
Alw21I GWGCWC 1 cut(s) 1614
Alw26I GTCTC 6 cut(s) 212, 702, 1951, 2065, 2081, 2405
AlwI GGATC 6 cut(s) 1047, 1060, 1449, 1907, 1920, 2540
AlwNI CAGNNNCTG 3 cut(s) 331, 700, 2057
AoxI GGCC 5 cut(s) 250, 524, 564, 812, 2035
ApaI GGGCCC 1 cut(s) 2039
ApeKI GCWGC 7 cut(s) 311, 491, 512, 908, 917, 1781, 2091
ApoI RAATTY 1 cut(s) 1087
AseI ATTAAT 1 cut(s) 83
Asp700I GAANNNNTTC 4 cut(s) 186, 287, 1674, 1722
AspLEI GCGC 1 cut(s) 1973
AspS9I GGNCC 9 cut(s) 813, 983, 1661, 2035, 2036, 2054, 2324, 2552, 2615
AsuC2I CCSGG 2 cut(s) 2235, 2550
AsuHPI GGTGA 3 cut(s) 1336, 1601, 2444
AsuII TTCGAA 2 cut(s) 647, 826
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 6 cut(s) 983, 1661, 2054, 2324, 2552, 2615
BaeGI GKGCMC 1 cut(s) 2039
BalI TGGCCA 1 cut(s) 566
BamHI GGATCC 2 cut(s) 1052, 1912
BanII GRGCYC 2 cut(s) 1614, 2039
BbsI GAAGAC 6 cut(s) 1151, 1472, 1960, 2103, 2160, 2478
Bbv12I GWGCWC 1 cut(s) 1614
BbvCI CCTCAGC 1 cut(s) 2457
BbvI GCAGC 7 cut(s) 323, 499, 503, 920, 929, 1793, 2103
BccI CCATC 4 cut(s) 414, 742, 1969, 2090
BceAI ACGGC 1 cut(s) 987
BcgI CGANNNNNNTGC 2 cut(s) 2087, 2121
BciT130I CCWGG 2 cut(s) 639, 818
BclI TGATCA 3 cut(s) 19, 265, 2209
BcnI CCSGG 2 cut(s) 2235, 2550
BcoDI GTCTC 6 cut(s) 212, 702, 1951, 2065, 2081, 2405
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 9 cut(s) 554, 791, 834, 935, 1196, 1518, 2181, 2409, 2484
BfmI CTRYAG 2 cut(s) 489, 1704
BfrI CTTAAG 1 cut(s) 1901
BfuAI ACCTGC 2 cut(s) 1186, 1239
BglII AGATCT 2 cut(s) 430, 1694
BisI GCNGC 8 cut(s) 312, 492, 513, 909, 918, 1736, 1782, 2092
BlpI GCTNAGC 2 cut(s) 616, 1967
BlsI GCNGC 8 cut(s) 313, 493, 514, 910, 919, 1737, 1783, 2093
BmcAI AGTACT 1 cut(s) 2185
Bme1390I CCNGG 4 cut(s) 639, 818, 2235, 2550
Bme18I GGWCC 6 cut(s) 983, 1661, 2054, 2324, 2552, 2615
BmgT120I GGNCC 9 cut(s) 813, 983, 1661, 2035, 2036, 2054, 2324, 2552, 2615
BmiI GGNNCC 7 cut(s) 815, 984, 1054, 1395, 1404, 1914, 2037
BmrFI CCNGG 4 cut(s) 639, 818, 2235, 2550
BmsI GCATC 5 cut(s) 700, 893, 895, 1936, 2110
BmtI GCTAGC 1 cut(s) 557
BpiI GAAGAC 6 cut(s) 1151, 1472, 1960, 2103, 2160, 2478
BplI GAGNNNNNCTC 2 cut(s) 2481, 2513
Bpu10I CCTNAGC 2 cut(s) 2346, 2457
Bpu1102I GCTNAGC 2 cut(s) 616, 1967
Bpu14I TTCGAA 2 cut(s) 647, 826
BpuEI CTTGAG 2 cut(s) 1414, 1715
BpuMI CCSGG 2 cut(s) 2235, 2550
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 5 cut(s) 369, 816, 1155, 1389, 2233
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 777, 807
Bsc4I CCNNNNNNNGG 5 cut(s) 533, 638, 1321, 2270, 2271
Bse1I ACTGG 4 cut(s) 855, 906, 1315, 1664
Bse3DI GCAATG 2 cut(s) 689, 1776
Bse8I GATNNNNATC 1 cut(s) 930
BseBI CCWGG 2 cut(s) 639, 818
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 5 cut(s) 369, 816, 1155, 1389, 2233
BseGI GGATG 4 cut(s) 472, 910, 1832, 2101
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 5 cut(s) 533, 638, 1321, 2270, 2271
BseMI GCAATG 2 cut(s) 689, 1776
BseMII CTCAG 7 cut(s) 306, 1958, 1962, 2076, 2360, 2471, 2561
BseNI ACTGG 4 cut(s) 855, 906, 1315, 1664
BseRI GAGGAG 3 cut(s) 1386, 2502, 2610
BseSI GKGCMC 1 cut(s) 2039
BseXI GCAGC 7 cut(s) 323, 499, 503, 920, 929, 1793, 2103
Bsh1236I CGCG 2 cut(s) 131, 1973
BshFI GGCC 5 cut(s) 252, 526, 566, 814, 2037
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 1614
BsiSI CCGG 3 cut(s) 798, 2235, 2550
BslFI GGGAC 2 cut(s) 996, 2431
BslI CCNNNNNNNGG 5 cut(s) 533, 638, 1321, 2270, 2271
BsmAI GTCTC 6 cut(s) 212, 702, 1951, 2065, 2081, 2405
BsmFI GGGAC 2 cut(s) 996, 2431
BsmI GAATGC 2 cut(s) 287, 1250
BsnI GGCC 5 cut(s) 252, 526, 566, 814, 2037
Bsp119I TTCGAA 2 cut(s) 647, 826
Bsp120I GGGCCC 1 cut(s) 2035
Bsp1286I GDGCHC 2 cut(s) 1614, 2039
Bsp1720I GCTNAGC 2 cut(s) 616, 1967
BspACI CCGC 2 cut(s) 1010, 1736
BspANI GGCC 5 cut(s) 252, 526, 566, 814, 2037
BspCNI CTCAG 7 cut(s) 307, 1959, 1961, 2075, 2359, 2470, 2562
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 2 cut(s) 131, 1973
BspHI TCATGA 2 cut(s) 1375, 2241
BspLI GGNNCC 7 cut(s) 815, 984, 1054, 1395, 1404, 1914, 2037
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 2 cut(s) 1186, 1239
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 6 cut(s) 1047, 1060, 1449, 1907, 1920, 2540
BspQI GCTCTTC 3 cut(s) 177, 1256, 2392
BspT104I TTCGAA 2 cut(s) 647, 826
BspTI CTTAAG 1 cut(s) 1901
BsrBI CCGCTC 1 cut(s) 1738
BsrDI GCAATG 2 cut(s) 689, 1776
BsrI ACTGG 4 cut(s) 855, 906, 1315, 1664
BssECI CCNNGG 5 cut(s) 369, 816, 1155, 1389, 2233
BssT1I CCWWGG 2 cut(s) 1155, 1389
Bst2UI CCWGG 2 cut(s) 639, 818
Bst4CI ACNGT 4 cut(s) 1209, 1945, 2556, 2600
Bst6I CTCTTC 4 cut(s) 177, 1256, 1704, 2392
BstAFI CTTAAG 1 cut(s) 1901
BstAPI GCANNNNNTGC 1 cut(s) 914
BstBI TTCGAA 2 cut(s) 647, 826
BstC8I GCNNGC 1 cut(s) 555
BstF5I GGATG 4 cut(s) 472, 910, 1832, 2101
BstFNI CGCG 2 cut(s) 131, 1973
BstHHI GCGC 1 cut(s) 1973
BstMAI GTCTC 6 cut(s) 212, 702, 1951, 2065, 2081, 2405
BstMWI GCNNNNNNNGC 7 cut(s) 14, 563, 612, 914, 917, 1400, 1803
BstNI CCWGG 2 cut(s) 639, 818
BstNSI RCATGY 1 cut(s) 1415
BstSCI CCNGG 4 cut(s) 637, 816, 2233, 2548
BstSFI CTRYAG 2 cut(s) 489, 1704
BstSLI GKGCMC 1 cut(s) 2039
BstUI CGCG 2 cut(s) 131, 1973
BstV1I GCAGC 7 cut(s) 323, 499, 503, 920, 929, 1793, 2103
BstV2I GAAGAC 6 cut(s) 1151, 1472, 1960, 2103, 2160, 2478
BstX2I RGATCY 5 cut(s) 430, 1052, 1441, 1694, 1912
BstYI RGATCY 5 cut(s) 430, 1052, 1441, 1694, 1912
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 5 cut(s) 252, 526, 566, 814, 2037
BsuTUI ATCGAT 1 cut(s) 234
BtgZI GCGATG 1 cut(s) 1947
BtsCI GGATG 4 cut(s) 472, 910, 1832, 2101
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 3 cut(s) 601, 1941, 2545
BveI ACCTGC 2 cut(s) 1186, 1239
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 3 cut(s) 331, 700, 2057
CciI TCATGA 2 cut(s) 1375, 2241
CfoI GCGC 1 cut(s) 1973
Cfr13I GGNCC 9 cut(s) 813, 983, 1661, 2035, 2036, 2054, 2324, 2552, 2615
ClaI ATCGAT 1 cut(s) 234
CpoI CGGWCCG 1 cut(s) 2552
CseI GACGC 3 cut(s) 2097, 2165, 2186
Csp6I GTAC 4 cut(s) 1854, 2184, 2302, 2413
CspCI CAANNNNNGTGG 2 cut(s) 1905, 1940
CspI CGGWCCG 1 cut(s) 2552
CviQI GTAC 4 cut(s) 1854, 2184, 2302, 2413
DraI TTTAAA 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 564
Eam1104I CTCTTC 4 cut(s) 177, 1256, 1704, 2392
EarI CTCTTC 4 cut(s) 177, 1256, 1704, 2392
Ecl136II GAGCTC 1 cut(s) 1612
Eco130I CCWWGG 2 cut(s) 1155, 1389
Eco147I AGGCCT 1 cut(s) 252
Eco24I GRGCYC 2 cut(s) 1614, 2039
Eco32I GATATC 2 cut(s) 1306, 1510
Eco47I GGWCC 6 cut(s) 983, 1661, 2054, 2324, 2552, 2615
Eco53kI GAGCTC 1 cut(s) 1612
Eco57I CTGAAG 4 cut(s) 1403, 1797, 2172, 2521
EcoICRI GAGCTC 1 cut(s) 1612
EcoO109I RGGNCCY 4 cut(s) 983, 2036, 2054, 2615
EcoRII CCWGG 2 cut(s) 637, 816
EcoRV GATATC 2 cut(s) 1306, 1510
EcoT14I CCWWGG 2 cut(s) 1155, 1389
EcoT38I GRGCYC 2 cut(s) 1614, 2039
ErhI CCWWGG 2 cut(s) 1155, 1389
FalI AAGNNNNNCTT 2 cut(s) 1710, 1742
FaqI GGGAC 2 cut(s) 996, 2431
FauNDI CATATG 2 cut(s) 279, 571
FbaI TGATCA 3 cut(s) 19, 265, 2209
FblI GTMKAC 1 cut(s) 704
Fnu4HI GCNGC 8 cut(s) 312, 492, 513, 909, 918, 1736, 1782, 2092
FokI GGATG 4 cut(s) 459, 917, 1819, 2088
FriOI GRGCYC 2 cut(s) 1614, 2039
Fsp4HI GCNGC 8 cut(s) 312, 492, 513, 909, 918, 1736, 1782, 2092
FspBI CTAG 9 cut(s) 554, 791, 834, 935, 1196, 1518, 2181, 2409, 2484
GlaI GCGC 1 cut(s) 1972
GluI GCNGC 8 cut(s) 312, 492, 513, 909, 918, 1736, 1782, 2092
HaeIII GGCC 5 cut(s) 252, 526, 566, 814, 2037
HapII CCGG 3 cut(s) 798, 2235, 2550
HgaI GACGC 3 cut(s) 2097, 2165, 2186
HhaI GCGC 1 cut(s) 1973
Hin6I GCGC 1 cut(s) 1971
HinP1I GCGC 1 cut(s) 1971
HincII GTYRAC 2 cut(s) 486, 1984
HindII GTYRAC 2 cut(s) 486, 1984
HindIII AAGCTT 1 cut(s) 613
HpaII CCGG 3 cut(s) 798, 2235, 2550
HphI GGTGA 3 cut(s) 1336, 1601, 2444
Hpy166II GTNNAC 4 cut(s) 486, 705, 1984, 2021
Hpy8I GTNNAC 4 cut(s) 486, 705, 1984, 2021
Hpy99I CGWCG 1 cut(s) 2301
HpyAV CCTTC 4 cut(s) 751, 1462, 1522, 1931
HpyCH4III ACNGT 4 cut(s) 1209, 1945, 2556, 2600
HpyCH4IV ACGT 5 cut(s) 1352, 1852, 2296, 2447, 2505
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 563, 612, 914, 917, 1400, 1803
HpySE526I ACGT 5 cut(s) 1352, 1852, 2296, 2447, 2505
HspAI GCGC 1 cut(s) 1971
Ksp22I TGATCA 3 cut(s) 19, 265, 2209
LguI GCTCTTC 3 cut(s) 177, 1256, 2392
LmnI GCTCC 5 cut(s) 1399, 1408, 1471, 1762, 2143
Lsp1109I GCAGC 7 cut(s) 323, 499, 503, 920, 929, 1793, 2103
LweI GCATC 5 cut(s) 700, 893, 895, 1936, 2110
MaeI CTAG 9 cut(s) 554, 791, 834, 935, 1196, 1518, 2181, 2409, 2484
MaeII ACGT 5 cut(s) 1352, 1852, 2296, 2447, 2505
MaeIII GTNAC 3 cut(s) 1651, 2292, 2540
MbiI CCGCTC 1 cut(s) 1738
MfeI CAATTG 1 cut(s) 1503
MflI RGATCY 5 cut(s) 430, 1052, 1441, 1694, 1912
MhlI GDGCHC 2 cut(s) 1614, 2039
MlsI TGGCCA 1 cut(s) 566
MluNI TGGCCA 1 cut(s) 566
MlyI GAGTC 4 cut(s) 357, 959, 1561, 2001
MmeI TCCRAC 3 cut(s) 199, 927, 1080
Mox20I TGGCCA 1 cut(s) 566
MroXI GAANNNNTTC 4 cut(s) 186, 287, 1674, 1722
MscI TGGCCA 1 cut(s) 566
MslI CAYNNNNRTG 3 cut(s) 111, 234, 1623
Msp20I TGGCCA 1 cut(s) 566
MspA1I CMGCKG 2 cut(s) 17, 314
MspCI CTTAAG 1 cut(s) 1901
MspI CCGG 3 cut(s) 798, 2235, 2550
MspR9I CCNGG 4 cut(s) 639, 818, 2235, 2550
MunI CAATTG 1 cut(s) 1503
Mva1269I GAATGC 2 cut(s) 287, 1250
MvaI CCWGG 2 cut(s) 639, 818
MvnI CGCG 2 cut(s) 131, 1973
MwoI GCNNNNNNNGC 7 cut(s) 14, 563, 612, 914, 917, 1400, 1803
NciI CCSGG 2 cut(s) 2235, 2550
NdeI CATATG 2 cut(s) 279, 571
NheI GCTAGC 1 cut(s) 553
NlaIV GGNNCC 7 cut(s) 815, 984, 1054, 1395, 1404, 1914, 2037
NmeAIII GCCGAG 1 cut(s) 2478
NmuCI GTSAC 1 cut(s) 2540
NspI RCATGY 1 cut(s) 1415
NspV TTCGAA 2 cut(s) 647, 826
PagI TCATGA 2 cut(s) 1375, 2241
PceI AGGCCT 1 cut(s) 252
PciSI GCTCTTC 3 cut(s) 177, 1256, 2392
PcsI WCGNNNNNNNCGW 1 cut(s) 1970
PctI GAATGC 2 cut(s) 287, 1250
PdmI GAANNNNTTC 4 cut(s) 186, 287, 1674, 1722
PfeI GAWTC 7 cut(s) 328, 374, 396, 1160, 2317, 2429, 2480
PfoI TCCNGGA 1 cut(s) 2548
PkrI GCNGC 8 cut(s) 313, 493, 514, 910, 919, 1737, 1783, 2093
PleI GAGTC 4 cut(s) 357, 958, 1561, 2001
PpsI GAGTC 4 cut(s) 357, 958, 1561, 2001
PpuMI RGGWCCY 3 cut(s) 983, 2054, 2615
PshBI ATTAAT 1 cut(s) 83
PsiI TTATAA 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 1614
Psp1406I AACGTT 3 cut(s) 1352, 2447, 2505
Psp5II RGGWCCY 3 cut(s) 983, 2054, 2615
Psp6I CCWGG 2 cut(s) 637, 816
PspGI CCWGG 2 cut(s) 637, 816
PspN4I GGNNCC 7 cut(s) 815, 984, 1054, 1395, 1404, 1914, 2037
PspOMI GGGCCC 1 cut(s) 2035
PspPI GGNCC 9 cut(s) 813, 983, 1661, 2035, 2036, 2054, 2324, 2552, 2615
PspPPI RGGWCCY 3 cut(s) 983, 2054, 2615
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 3 cut(s) 331, 700, 2057
PsuI RGATCY 5 cut(s) 430, 1052, 1441, 1694, 1912
PvuII CAGCTG 2 cut(s) 17, 314
RsaI GTAC 4 cut(s) 1855, 2185, 2303, 2414
RsaNI GTAC 4 cut(s) 1854, 2184, 2302, 2413
RseI CAYNNNNRTG 3 cut(s) 111, 234, 1623
Rsr2I CGGWCCG 1 cut(s) 2552
RsrII CGGWCCG 1 cut(s) 2552
SacI GAGCTC 1 cut(s) 1614
SapI GCTCTTC 3 cut(s) 177, 1256, 2392
SatI GCNGC 8 cut(s) 312, 492, 513, 909, 918, 1736, 1782, 2092
Sau96I GGNCC 9 cut(s) 813, 983, 1661, 2035, 2036, 2054, 2324, 2552, 2615
ScaI AGTACT 1 cut(s) 2185
SchI GAGTC 4 cut(s) 357, 959, 1561, 2001
ScrFI CCNGG 4 cut(s) 639, 818, 2235, 2550
SduI GDGCHC 2 cut(s) 1614, 2039
SfaNI GCATC 5 cut(s) 700, 893, 895, 1936, 2110
SfcI CTRYAG 2 cut(s) 489, 1704
SfuI TTCGAA 2 cut(s) 647, 826
SinI GGWCC 6 cut(s) 983, 1661, 2054, 2324, 2552, 2615
SmiMI CAYNNNNRTG 3 cut(s) 111, 234, 1623
SmlI CTYRAG 3 cut(s) 1429, 1730, 1901
SmoI CTYRAG 3 cut(s) 1429, 1730, 1901
SpeI ACTAGT 1 cut(s) 790
SseBI AGGCCT 1 cut(s) 252
SsiI CCGC 2 cut(s) 1010, 1736
SspMI CTAG 9 cut(s) 554, 791, 834, 935, 1196, 1518, 2181, 2409, 2484
SstI GAGCTC 1 cut(s) 1614
StuI AGGCCT 1 cut(s) 252
StyD4I CCNGG 4 cut(s) 637, 816, 2233, 2548
StyI CCWWGG 2 cut(s) 1155, 1389
TaaI ACNGT 4 cut(s) 1209, 1945, 2556, 2600
TaiI ACGT 5 cut(s) 1355, 1855, 2299, 2450, 2508
TaqI TCGA 5 cut(s) 143, 234, 647, 826, 1932
TatI WGTACW 1 cut(s) 2183
TauI GCSGC 1 cut(s) 1738
TfiI GAWTC 7 cut(s) 328, 374, 396, 1160, 2317, 2429, 2480
TscAI CASTG 3 cut(s) 608, 1948, 2545
TseFI GTSAC 1 cut(s) 2540
TseI GCWGC 7 cut(s) 311, 491, 512, 908, 917, 1781, 2091
Tsp45I GTSAC 1 cut(s) 2540
TspDTI ATGAA 9 cut(s) 296, 483, 1392, 1607, 1704, 2061, 2159, 2258, 2493
TspGWI ACGGA 6 cut(s) 1501, 1579, 1620, 1925, 2043, 2121
TspRI CASTG 3 cut(s) 608, 1948, 2545
Vha464I CTTAAG 1 cut(s) 1901
VpaK11BI GGWCC 6 cut(s) 983, 1661, 2054, 2324, 2552, 2615
VspI ATTAAT 1 cut(s) 83
XapI RAATTY 1 cut(s) 1087
XbaI TCTAGA 3 cut(s) 934, 1195, 2483
XceI RCATGY 1 cut(s) 1415
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 4 cut(s) 186, 287, 1674, 1722
XspI CTAG 9 cut(s) 554, 791, 834, 935, 1196, 1518, 2181, 2409, 2484
ZrmI AGTACT 1 cut(s) 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.