FvH4_3g09000

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002793
Physical Location & Seq
Reverse (-)
5257849 .. 5262977
5129 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09000.t10

Sequence Viewer

Length: 2898 bp
ATGATGGCTAATCCGCAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGTTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGACTTGTACCGGAAAGTGAGATTTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGATTTTCGTAAAAGAATAAAGAGAAGTTCAGCAGCGCCTCGACATGATACTTATCTAGAAGGATTTAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCAATGGCCTCACAGAAGAGTCTGTACTTTACAGAAAGGGAGGCACATTTACCTCTGGGGAAAAAAGGGAGTTGCAAAAGTGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGAAGTCCAAAGCCGAAATTGGTCAGTTGCAGCCACCCACTACTTGGAAGCAACAAGAATTTGGCCAGACAGTGGAAACCCCCAAAATCAGTTAGCTGTATTGGCGATGTACATTGGTGATGAATTCCTCGCTTTATACCACTGTATACGAAGTCTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTCTTTGAAAAGAACAGGTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTAAATCCATCTGAAAGAAGCAGTGTTCAGATTACTAAGCAGGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAGATTTATGGCCTCTTATGATCAGGACGCTTAGTTTCCTCCACTTGAAATTGAGTGTGGATGAATTCCCTCGTGCTTTCGCATCTACTATGAAGGAGTTGGATGCATTGATGGCATTAGACGATACAAAACTAAATGCCCCTTTGGAATCATATCAGCGCATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATTTTTATCATACAGAATCTAGTTAAGAGGCCAGAAACAGAAACAATAGACTTGCAGAAGCAAACTGATATGCATCAGATGGAGTTGACACAGTTGGCATTGACTGCCACATTTATTTTTATGGGACGCTGTGTTGAAAGATGTTTGAAGGCTAGCACAATCGAAACCTGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGCTGGTCTTCATTTTTGATGAAGCAGAAACCTATGGGGTTGATGAGAAAAGTAGGTGTGCTATGTCCTACTTTTTTGGTGAATTTTTTAATCTCCTAAAGCGATTGAATGTCAATGGCGGTGAGGTAAAGTATACAGAAGGTGTTCCTTTGTGGGAAGACCATGAGCTGAGGGGCTTTGCACCACTAGCTACTTCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATGGACAATTATGAAAGTGGAATGGATTATCGTTCTCAGCGTATCATCAATGCCGCAATCAAAATTGCAGACAGATCAACTGATTCTCAAAAGTGGATTGCCTATGACAAGTCTGAAAGAAAATTTTGCAAATGCCTCGTGACAGGGTCAAATGGATATCCGGACAAGAAAGGGTCAGGAAGATTGGAGTCCAACAACTCCGATGTTGAATTGAATATACTTGGTGAGAAGATTGACAAAGCACCAGAAGAATGTGAGAAACTGATGAGTGATGGGGAAAATCCAAGCTCTATTTCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAATCAGTATTGCCTCTACTTTGAAGGACCCAACCTCTCCGAAACATTCATTGGATCAAAATGTACCTTCGGATGAATGCTTGCGCCGTGCTACTTCACTTCTTATAGCACAAAACCCAGCTCAGAGTGATCCTTATTCTTTCCATATTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCAGCAGCAGCCTGTTGTGACAGATACAATAGCACAGCCAGTTTCGGAAACTCCAGTAGCTGCTGGGCCTCCCTCCCTTAATGCTTGGGTCTTTGACAGGGGCAGTTTGAGCAACGGCAGGGAGAAAAGCACAGATGGAGCAAGTAAACATGGCTCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCCATAAATGGCAATGAAGATTCTTTCAGCCATCACGAATGTGCAAGCACCCTATCCTCTTTGGCTTCTTATACAGCTCCTGTGCCTTCAGCCCCTCCTTTAGTGCTAGATGATGATCGCATTTGGTTTAATGAGGGGATCAGCATGGCCAATAATGCTTCTGATGTATCCTATTCAGAAGTAACCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTAATTTCAGTCCTATCATTCCGAGTTTCATAGACAAATACCCAACTCAACATCGAATGACTTCATCTGAATGGCTTCGTCAGTATAGAGAAAGTCATAACCTGGAGCATCATGGTTGGCCAAATTATGTTCATCCTCCTAGTAACCTTGGAAACTTGTACGGTTATGATACTTCAAAGTTTCACCATTTCAGTCAATGGGGAACTCCTGAGGCTTCTAGTCCCTCGACATTGCATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCCATATGCGTGTCGAGCTCTGACAGACATAAGAAATGAGCAGCAACCACTTCTTCAGTACCTTAAGGAGAGGGAAAAGCAACTCCAGAGAGATCCAACTGTGAGAGGCCCTTCTTATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

966

Amino Acids

110.18

Weight (kDa)

5.7

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 66 - 171 8.6e-15 Telomerase activating protein Est1
EST1_DNA_bind PF10373 183 - 493 1.7e-54 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1253
AccB7I CCANNNNNTGG 2 cut(s) 562, 590
AccI GTMKAC 2 cut(s) 664, 1415
AccIII TCCGGA 1 cut(s) 1675
AciI CCGC 3 cut(s) 14, 1401, 1569
AclWI GGATC 7 cut(s) 79, 492, 1929, 1991, 2411, 2732, 2855
AcoI YGGCCR 3 cut(s) 581, 2412, 2603
AcsI RAATTY 7 cut(s) 324, 340, 576, 641, 926, 1364, 1637
AcuI CTGAAG 5 cut(s) 339, 540, 2229, 2337, 2807
AfaI GTAC 7 cut(s) 128, 390, 629, 1074, 1932, 2645, 2828
AfiI CCNNNNNNNGG 3 cut(s) 562, 590, 2233
AflII CTTAAG 1 cut(s) 2831
AflIII ACRYGT 1 cut(s) 2040
AjnI CCWGG 4 cut(s) 1059, 2586, 2722, 2740
AjuI GAANNNNNNNTTGG 6 cut(s) 1792, 1824, 1901, 1933, 2241, 2273
AleI CACNNNNGTG 1 cut(s) 2304
Alw21I GWGCWC 2 cut(s) 1059, 2788
AlwI GGATC 7 cut(s) 79, 492, 1929, 1991, 2411, 2732, 2855
AlwNI CAGNNNCTG 2 cut(s) 2120, 2345
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 1675
ApoI RAATTY 7 cut(s) 324, 340, 576, 641, 926, 1364, 1637
Asp700I GAANNNNTTC 5 cut(s) 324, 1425, 1830, 2545, 2559
AspLEI GCGC 4 cut(s) 274, 1023, 1953, 2037
AspS9I GGNCC 5 cut(s) 1043, 1894, 2126, 2480, 2876
AsuHPI GGTGA 6 cut(s) 647, 706, 1373, 1415, 1751, 2660
AsuNHI GCTAGC 1 cut(s) 1229
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 1894
AxyI CCTNAGG 2 cut(s) 696, 2694
BaeGI GKGCMC 1 cut(s) 82
BalI TGGCCA 3 cut(s) 583, 2414, 2605
BanII GRGCYC 2 cut(s) 2228, 2788
BarI GAAGNNNNNNTAC 2 cut(s) 372, 404
BauI CACGAG 2 cut(s) 933, 1652
BbsI GAAGAC 2 cut(s) 1282, 1446
Bbv12I GWGCWC 2 cut(s) 1059, 2788
BbvCI CCTCAGC 1 cut(s) 1451
BccI CCATC 7 cut(s) 468, 799, 967, 1150, 1781, 2189, 2304
BceAI ACGGC 2 cut(s) 1938, 2191
BcgI CGANNNNNNTGC 2 cut(s) 1633, 1667
BciT130I CCWGG 4 cut(s) 1061, 2588, 2724, 2742
BciVI GTATCC 2 cut(s) 853, 2443
BclI TGATCA 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 275
BfrI CTTAAG 1 cut(s) 2831
BfuAI ACCTGC 1 cut(s) 1253
BfuI GTATCC 2 cut(s) 853, 2443
Bme1390I CCNGG 4 cut(s) 1061, 2588, 2724, 2742
Bme18I GGWCC 1 cut(s) 1894
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 5 cut(s) 1043, 1894, 2126, 2480, 2876
BmiI GGNNCC 1 cut(s) 1896
BmrFI CCNGG 4 cut(s) 1061, 2588, 2724, 2742
BmsI GCATC 5 cut(s) 953, 955, 1159, 2602, 2728
BmtI GCTAGC 1 cut(s) 1233
BpiI GAAGAC 2 cut(s) 1282, 1446
BpmI CTGGAG 4 cut(s) 1043, 2097, 2609, 2837
Bpu10I CCTNAGC 1 cut(s) 1451
BpuEI CTTGAG 2 cut(s) 2200, 2460
BsaBI GATNNNNATC 4 cut(s) 288, 1082, 1149, 2379
BsaJI CCNNGG 3 cut(s) 478, 702, 2632
BsaWI WCCGGW 2 cut(s) 129, 1675
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 590, 2233
Bse1I ACTGG 2 cut(s) 2099, 2114
Bse21I CCTNAGG 2 cut(s) 696, 2694
Bse3DI GCAATG 4 cut(s) 373, 838, 2284, 2714
Bse8I GATNNNNATC 4 cut(s) 288, 1082, 1149, 2379
BseAI TCCGGA 1 cut(s) 1675
BseBI CCWGG 4 cut(s) 1061, 2588, 2724, 2742
BseDI CCNNGG 3 cut(s) 478, 702, 2632
BseGI GGATG 6 cut(s) 159, 928, 970, 1945, 2617, 2719
BseJI GATNNNNATC 4 cut(s) 288, 1082, 1149, 2379
BseLI CCNNNNNNNGG 3 cut(s) 562, 590, 2233
BseMI GCAATG 4 cut(s) 373, 838, 2284, 2714
BseMII CTCAG 5 cut(s) 687, 1442, 1565, 2003, 2685
BseNI ACTGG 2 cut(s) 2099, 2114
BseSI GKGCMC 1 cut(s) 82
BseYI CCCAGC 2 cut(s) 1984, 2123
BsiHKAI GWGCWC 2 cut(s) 1059, 2788
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 130, 1676
BslFI GGGAC 2 cut(s) 1215, 2691
BslI CCNNNNNNNGG 3 cut(s) 562, 590, 2233
BsmFI GGGAC 2 cut(s) 1215, 2691
BsmI GAATGC 2 cut(s) 368, 1949
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 4 cut(s) 82, 1059, 2228, 2788
Bsp13I TCCGGA 1 cut(s) 1675
Bsp1407I TGTACA 1 cut(s) 627
BspACI CCGC 3 cut(s) 14, 1401, 1569
BspCNI CTCAG 5 cut(s) 688, 1443, 1564, 2002, 2686
BspEI TCCGGA 1 cut(s) 1675
BspLI GGNNCC 1 cut(s) 1896
BspMI ACCTGC 1 cut(s) 1253
BspOI GCTAGC 1 cut(s) 1233
BspPI GGATC 7 cut(s) 79, 492, 1929, 1991, 2411, 2732, 2855
BspTI CTTAAG 1 cut(s) 2831
BsrDI GCAATG 4 cut(s) 373, 838, 2284, 2714
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 2 cut(s) 2099, 2114
BssECI CCNNGG 3 cut(s) 478, 702, 2632
BssNAI GTATAC 2 cut(s) 665, 1416
BssSI CACGAG 2 cut(s) 933, 1652
BssT1I CCWWGG 2 cut(s) 702, 2632
Bst1107I GTATAC 2 cut(s) 665, 1416
Bst2BI CACGAG 2 cut(s) 933, 1652
Bst2UI CCWGG 4 cut(s) 1061, 2588, 2724, 2742
Bst4CI ACNGT 5 cut(s) 590, 662, 1170, 2648, 2869
Bst6I CTCTTC 2 cut(s) 375, 1812
BstAFI CTTAAG 1 cut(s) 2831
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 4 cut(s) 58, 1231, 1949, 2311
BstDEI CTNAG 7 cut(s) 696, 819, 893, 1451, 1551, 1989, 2694
BstEII GGTNACC 1 cut(s) 2753
BstF5I GGATG 6 cut(s) 159, 928, 970, 1945, 2617, 2719
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 4 cut(s) 274, 1023, 1953, 2037
BstNI CCWGG 4 cut(s) 1061, 2588, 2724, 2742
BstNSI RCATGY 2 cut(s) 1484, 2044
BstPI GGTNACC 1 cut(s) 2753
BstSCI CCNGG 4 cut(s) 1059, 2586, 2722, 2740
BstSLI GKGCMC 1 cut(s) 82
BstV2I GAAGAC 2 cut(s) 1282, 1446
BstX2I RGATCY 3 cut(s) 484, 2737, 2860
BstYI RGATCY 3 cut(s) 484, 2737, 2860
BstZ17I GTATAC 2 cut(s) 665, 1416
Bsu36I CCTNAGG 2 cut(s) 696, 2694
BsuI GTATCC 2 cut(s) 853, 2443
BtgZI GCGATG 2 cut(s) 446, 638
BtsCI GGATG 6 cut(s) 159, 928, 970, 1945, 2617, 2719
BtsI GCAGTG 1 cut(s) 811
BtsIMutI CAGTG 4 cut(s) 80, 595, 658, 811
BveI ACCTGC 1 cut(s) 1253
Cac8I GCNNGC 4 cut(s) 58, 1231, 1949, 2311
CaiI CAGNNNCTG 2 cut(s) 2120, 2345
CfoI GCGC 4 cut(s) 274, 1023, 1953, 2037
Cfr13I GGNCC 5 cut(s) 1043, 1894, 2126, 2480, 2876
CseI GACGC 2 cut(s) 898, 1212
Csp6I GTAC 7 cut(s) 127, 389, 628, 1073, 1931, 2644, 2827
CviQI GTAC 7 cut(s) 127, 389, 628, 1073, 1931, 2644, 2827
DdeI CTNAG 7 cut(s) 696, 819, 893, 1451, 1551, 1989, 2694
DraI TTTAAA 1 cut(s) 304
EaeI YGGCCR 3 cut(s) 581, 2412, 2603
Eam1104I CTCTTC 2 cut(s) 375, 1812
EarI CTCTTC 2 cut(s) 375, 1812
Ecl136II GAGCTC 1 cut(s) 2786
Eco130I CCWWGG 2 cut(s) 702, 2632
Eco147I AGGCCT 1 cut(s) 1843
Eco24I GRGCYC 2 cut(s) 2228, 2788
Eco32I GATATC 1 cut(s) 1673
Eco47I GGWCC 1 cut(s) 1894
Eco53kI GAGCTC 1 cut(s) 2786
Eco57I CTGAAG 5 cut(s) 339, 540, 2229, 2337, 2807
Eco81I CCTNAGG 2 cut(s) 696, 2694
Eco88I CYCGRG 1 cut(s) 479
Eco91I GGTNACC 1 cut(s) 2753
EcoICRI GAGCTC 1 cut(s) 2786
EcoO109I RGGNCCY 3 cut(s) 1043, 1894, 2876
EcoO65I GGTNACC 1 cut(s) 2753
EcoRI GAATTC 3 cut(s) 324, 641, 926
EcoRII CCWGG 4 cut(s) 1059, 2586, 2722, 2740
EcoRV GATATC 1 cut(s) 1673
EcoT14I CCWWGG 2 cut(s) 702, 2632
EcoT22I ATGCAT 3 cut(s) 970, 1152, 1486
EcoT38I GRGCYC 2 cut(s) 2228, 2788
ErhI CCWWGG 2 cut(s) 702, 2632
FalI AAGNNNNNCTT 2 cut(s) 1818, 1850
FaqI GGGAC 2 cut(s) 1215, 2691
FauNDI CATATG 2 cut(s) 1515, 2773
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 2 cut(s) 664, 1415
FokI GGATG 6 cut(s) 146, 935, 977, 1952, 2604, 2706
FriOI GRGCYC 2 cut(s) 2228, 2788
GlaI GCGC 4 cut(s) 273, 1022, 1952, 2036
GsaI CCCAGC 2 cut(s) 1988, 2127
GsuI CTGGAG 4 cut(s) 1043, 2097, 2609, 2837
HaeII RGCGCY 1 cut(s) 275
HapII CCGG 2 cut(s) 130, 1676
HgaI GACGC 2 cut(s) 898, 1212
HhaI GCGC 4 cut(s) 274, 1023, 1953, 2037
Hin6I GCGC 4 cut(s) 272, 1021, 1951, 2035
HinP1I GCGC 4 cut(s) 272, 1021, 1951, 2035
HincII GTYRAC 1 cut(s) 1164
HindII GTYRAC 1 cut(s) 1164
HindIII AAGCTT 2 cut(s) 215, 227
HpaII CCGG 2 cut(s) 130, 1676
HphI GGTGA 6 cut(s) 647, 706, 1373, 1415, 1751, 2660
Hpy166II GTNNAC 4 cut(s) 665, 1164, 1416, 2207
Hpy8I GTNNAC 4 cut(s) 665, 1164, 1416, 2207
HpyCH4III ACNGT 5 cut(s) 590, 662, 1170, 2648, 2869
HpyCH4IV ACGT 1 cut(s) 1855
HpyF3I CTNAG 7 cut(s) 696, 819, 893, 1451, 1551, 1989, 2694
HpySE526I ACGT 1 cut(s) 1855
HspAI GCGC 4 cut(s) 272, 1021, 1951, 2035
Kpn2I TCCGGA 1 cut(s) 1675
Ksp22I TGATCA 1 cut(s) 882
LmnI GCTCC 4 cut(s) 1062, 2198, 2347, 2590
LweI GCATC 5 cut(s) 953, 955, 1159, 2602, 2728
MaeII ACGT 1 cut(s) 1855
MaeIII GTNAC 5 cut(s) 1654, 2077, 2446, 2627, 2753
MfeI CAATTG 1 cut(s) 2893
MflI RGATCY 3 cut(s) 484, 2737, 2860
MhlI GDGCHC 4 cut(s) 82, 1059, 2228, 2788
MlsI TGGCCA 3 cut(s) 583, 2414, 2605
MluNI TGGCCA 3 cut(s) 583, 2414, 2605
MlyI GAGTC 3 cut(s) 392, 1712, 2017
MmeI TCCRAC 3 cut(s) 942, 1731, 2888
Mox20I TGGCCA 3 cut(s) 583, 2414, 2605
Mph1103I ATGCAT 3 cut(s) 970, 1152, 1486
MroI TCCGGA 1 cut(s) 1675
MroXI GAANNNNTTC 5 cut(s) 324, 1425, 1830, 2545, 2559
MscI TGGCCA 3 cut(s) 583, 2414, 2605
MseI TTAA 9 cut(s) 303, 681, 777, 785, 1101, 1371, 2139, 2394, 2832
MslI CAYNNNNRTG 4 cut(s) 111, 2304, 2554, 2776
Msp20I TGGCCA 3 cut(s) 583, 2414, 2605
MspA1I CMGCKG 2 cut(s) 181, 1262
MspCI CTTAAG 1 cut(s) 2831
MspI CCGG 2 cut(s) 130, 1676
MspR9I CCNGG 4 cut(s) 1061, 2588, 2724, 2742
MunI CAATTG 1 cut(s) 2893
Mva1269I GAATGC 2 cut(s) 368, 1949
MvaI CCWGG 4 cut(s) 1061, 2588, 2724, 2742
NdeI CATATG 2 cut(s) 1515, 2773
NheI GCTAGC 1 cut(s) 1229
NlaIV GGNNCC 1 cut(s) 1896
NmuCI GTSAC 2 cut(s) 1654, 2077
NsiI ATGCAT 3 cut(s) 970, 1152, 1486
NspI RCATGY 2 cut(s) 1484, 2044
OliI CACNNNNGTG 1 cut(s) 2304
PceI AGGCCT 1 cut(s) 1843
PciI ACATGT 1 cut(s) 2040
PcsI WCGNNNNNNNCGW 1 cut(s) 1552
PctI GAATGC 2 cut(s) 368, 1949
PdmI GAANNNNTTC 5 cut(s) 324, 1425, 1830, 2545, 2559
PfeI GAWTC 7 cut(s) 67, 1010, 1028, 1093, 1598, 2249, 2285
PflFI GACNNNGTC 1 cut(s) 1660
PflMI CCANNNNNTGG 2 cut(s) 562, 590
PleI GAGTC 3 cut(s) 391, 1711, 2017
PpsI GAGTC 3 cut(s) 391, 1711, 2017
PpuMI RGGWCCY 1 cut(s) 1894
PscI ACATGT 1 cut(s) 2040
Psp124BI GAGCTC 1 cut(s) 2788
Psp5II RGGWCCY 1 cut(s) 1894
Psp6I CCWGG 4 cut(s) 1059, 2586, 2722, 2740
PspEI GGTNACC 1 cut(s) 2753
PspFI CCCAGC 2 cut(s) 1984, 2123
PspGI CCWGG 4 cut(s) 1059, 2586, 2722, 2740
PspN4I GGNNCC 1 cut(s) 1896
PspPI GGNCC 5 cut(s) 1043, 1894, 2126, 2480, 2876
PspPPI RGGWCCY 1 cut(s) 1894
PstNI CAGNNNCTG 2 cut(s) 2120, 2345
PsuI RGATCY 3 cut(s) 484, 2737, 2860
PsyI GACNNNGTC 1 cut(s) 1660
PvuII CAGCTG 2 cut(s) 181, 1262
RsaI GTAC 7 cut(s) 128, 390, 629, 1074, 1932, 2645, 2828
RsaNI GTAC 7 cut(s) 127, 389, 628, 1073, 1931, 2644, 2827
RseI CAYNNNNRTG 4 cut(s) 111, 2304, 2554, 2776
SacI GAGCTC 1 cut(s) 2788
SaqAI TTAA 9 cut(s) 303, 681, 777, 785, 1101, 1371, 2139, 2394, 2832
Sau96I GGNCC 5 cut(s) 1043, 1894, 2126, 2480, 2876
SchI GAGTC 3 cut(s) 392, 1712, 2017
ScrFI CCNGG 4 cut(s) 1061, 2588, 2724, 2742
SduI GDGCHC 4 cut(s) 82, 1059, 2228, 2788
SfaNI GCATC 5 cut(s) 953, 955, 1159, 2602, 2728
SinI GGWCC 1 cut(s) 1894
SmiMI CAYNNNNRTG 4 cut(s) 111, 2304, 2554, 2776
SmlI CTYRAG 3 cut(s) 2215, 2475, 2831
SmoI CTYRAG 3 cut(s) 2215, 2475, 2831
SseBI AGGCCT 1 cut(s) 1843
SsiI CCGC 3 cut(s) 14, 1401, 1569
SstI GAGCTC 1 cut(s) 2788
StuI AGGCCT 1 cut(s) 1843
StyD4I CCNGG 4 cut(s) 1059, 2586, 2722, 2740
StyI CCWWGG 2 cut(s) 702, 2632
TaaI ACNGT 5 cut(s) 590, 662, 1170, 2648, 2869
TaiI ACGT 1 cut(s) 1858
TaqI TCGA 6 cut(s) 277, 1051, 1239, 2539, 2711, 2782
TatI WGTACW 3 cut(s) 388, 627, 1072
TauI GCSGC 1 cut(s) 1571
TfiI GAWTC 7 cut(s) 67, 1010, 1028, 1093, 1598, 2249, 2285
Tru1I TTAA 9 cut(s) 303, 681, 777, 785, 1101, 1371, 2139, 2394, 2832
Tru9I TTAA 9 cut(s) 303, 681, 777, 785, 1101, 1371, 2139, 2394, 2832
TscAI CASTG 4 cut(s) 87, 595, 665, 811
TseFI GTSAC 2 cut(s) 1654, 2077
Tsp45I GTSAC 2 cut(s) 1654, 2077
TspRI CASTG 4 cut(s) 87, 595, 665, 811
Tth111I GACNNNGTC 1 cut(s) 1660
Van91I CCANNNNNTGG 2 cut(s) 562, 590
Vha464I CTTAAG 1 cut(s) 2831
VpaK11BI GGWCC 1 cut(s) 1894
XapI RAATTY 7 cut(s) 324, 340, 576, 641, 926, 1364, 1637
XbaI TCTAGA 2 cut(s) 292, 2734
XceI RCATGY 2 cut(s) 1484, 2044
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XmiI GTMKAC 2 cut(s) 664, 1415
XmnI GAANNNNTTC 5 cut(s) 324, 1425, 1830, 2545, 2559
Zsp2I ATGCAT 3 cut(s) 970, 1152, 1486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.