pycom05g28930

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002793
Physical Location & Seq
Reverse (-)
29482233 .. 29486001
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28930.1

Sequence Viewer

Length: 3009 bp
ATGAAGGCGAATTCACCTTTGCCCCTCAAGGATCAGAGAGAAAGACAGAAGTTTTTGATTGAGGTTGCAATCAAAGAAAACCAGTTGTGGGGTATGGTCCATTCCAAAGGTCTTTTGAACTCTGAGGTTCAGGACTTGTACCGCAAAGTGCGATCTAGTTATGAGAATCTCATACTGAGTGATTGTGAGCAGTTGGACCTTCAAGACATCGAGTATTCTTTGTGGAAGCTTCACTACAAGCATATTGATGAGTTTCGCAAAAGAATAAAGGGAAGTTCAGTGAATGCAGAAAGCAAAAATAAGGTGGTGCTACGAAATGATATTCATATAGAAGGGTTTAAAGTCTTTCTATCCGAAGCAATTGAATTTTACCAGAATGTGACTGTGAAATTAGGAAAATGTAAGAGTCTCCCAGAAGAGTCCGTGATAAATAGAAAGGAAGGCGACCTTACTTTTGCAGAACAGGAAAAGATGCAGAAGTGCCAGTTTTTGTGCCATCGCTTCTTGGTATGTCTTGGGGATCTGGCTAGGTACAGAGAACAATATGAAAAGCCTGATGTGCAAACCCGAAATTGGTCAGTTGCGGCCACTCACTACTTGGAAGCGGCAGCAACTTGGTCAGATAGTGGAAACCCCCACAATCAGTTGGCTGTATTGGCAATATATATTGGTGATGAATTACTCGCTTTGTACCATTGTATAAGAAGTTTAGCTGTTAAAGAACCTTTCCCTGACGCCCAAGATAACCTCATTCTACTGTTTGAAAGGAGCAGGTCATCTCATGTATATTCTCTTTCAAGCGAGGTCCGCTTTAATTTCTTAAACCCATCTGAAAGAAGCGGTGGTCAGACTATTTCACAGTCGAGCGATGATAATATGGTGAAAGCTGAGAATGTTTGCTTCATGGATACAAAGTTATGGTCTCTTATCATTGGAACGCTAAGTTTCTTTCACGTTAAATCAAGTGTAGATGAATTCCCCTGTGCTTTTGCATCTACTATGCGAGAGTTGGATGCACTGATTGCACTAGATGATACAGAGCTAAAAGCCACATTGGAGTCATATCAACGTATGGATTCGGTTAGAAGAGGCCCATTTCGAGCACTCCAAGTTGTTTCTGTACTAATCTTTACCGTACAGAATCTAATCAAGACCCCTGAAATAAAAGAATCAACAGACAAAATTGACATCCAGCAAAAAGAGCTGACACAGTTGGCACTGAATGCAGCATTCATTTTCATGGGGCGCTTTGTTGAAAGATGTTCAGAGGCTGGTCCAGTCGAGACTTGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAGTGGCTAGTCGTCGTGCTGAGTGGAGCAGAAACGTATGGGGTCGATGAAAAAAGCAGAAGTGCGATGTCTTACTTTTTTGGTGCATTTGTTGATCTTCTGAAGCGATTCAACCTCAGCGAGGATGAGGCCAAGTGTCCAGAAGGTACTCCTTTGTGGGAAGACTATGAGCTGAGGGGATTTGCTCCGGTAGCTTGTGTTCATGCATCACTAGATTTCTTGTACAATCGGGAACATATAGACAATTATGACAGCGGAATTGATTGTCGTGCTCAGAGAATCATCAAGGCTGCAATGAAAATTGCAGACAGATCAACTGGTTCTCAAAAGTGGATTGTCTATGACAAATCTCAAAGAAAATTTTCCAAAGCCGATATGGCAGAGTCAAATGAATATGCTGACGGGAAAGAACTAGGAGGACTTGAGTCCAAGAATTCTGATGGTAAACTGAAAGTGCCTAGTCAGCATATCCCCGAAGCGCTGAAAAAATGCGAGAAACAGATGATTGGGGAAAACCCAAGTTCGACCAGACAATCTGTTGACACGGAAGAGGAAGAAGTTATTCTCTTCAGGCCTCTTACAAGACATAACTCAGCACCACTCAAGATTTCCTCTCCTTTAAAGGATCCTAACTCTACGAAAGATATGGCAGATCAAAGTGTACCTTCTGATGAGTGCTTGCGCCGTGCCACATCACTTCTTATAGCGCAAAACAAGGCAAAGACTGATCCTTTATCTTCTCATGCCAACATCGCTAATTTTTGGCGCAACATGCCATTTAATCAGCAGGAGCCTTCTGTGAAGGAACAGCCACCAATAACCGCGGGGCCTCCCTCTCTTAATGCTTGGGTCCTGGACGGAGGAAATTTGAACAGTAATAAGGACAAAAGCACAAGCGGTATTAGTAAATGTGGCTCGAGATTGAGCCCCATTGAGGAAATAGCTTCGGAATTTGTGGATGTTCTGTCCATCAGTGAAAATGAATATTCTATCGGCAGTCACGAAATTTCGAGCACTCAATCCTCTTCCTCTACTTATACAACTCCTCTGCCTTCTGCTCCTTTGTTGCCAGATGACGCTGTTTGGTTTAATGGTGGAACCGAATCTAGTTTCATCGACTGCAAAAGCTCCGCAGGGATCAGCATGACCGACAATCTCTGTGACGCTGACGTGACACATTCACAGGCAGGCAGCTATGCAAACTGGACTGCAACTCAAGGGCTACCAGATTATAGTTCCGGCATTCCAAATTTTGTGGATAAGTACCCGCCTTGGCATCAAATGACATCATCTGAATGGCTGCGTCAGTACAGAGAAAGTCTCAACCTTGGCCATAATGCTTGGCCAAATTCTCTTTATCCGCCCAGGAACCTCGGAAACTTGTCCGATTATGATGCTACCAGGTTAAATCATTTCAACCGATGGGGACATCACATGGCTTCTAATCCGCCAATGTGTACAAACAACCCTCCGCCATTGGTTCCTCCTTTTCCTCTAGATTATGGTGATGCAGATGGGCAAAGAAGGGAGAAGCTTCTTCCTGGCTATCAAAGAACAAGCCCTTACGGGTGTGGCGCTGTGAGGGATGTAAGAAACGAGCAGCGGCCGCTTCTGCAGTACCTTAAGGAGTTAGAAAGGAAAGTGCAGCATGATCCCACTTTCAGAGGTCCTAGTTATTTGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

1003

Amino Acids

113.03

Weight (kDa)

5.59

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 70 - 183 1.4e-14 Telomerase activating protein Est1
EST1_DNA_bind PF10373 195 - 506 1.4e-52 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 762
AccII CGCG 1 cut(s) 2144
AclWI GGATC 7 cut(s) 39, 528, 1940, 1953, 2042, 2465, 2966
AcoI YGGCCR 4 cut(s) 585, 2650, 2663, 2924
AcuI CTGAAG 2 cut(s) 1442, 1873
AcyI GRCGYC 1 cut(s) 735
AfeI AGCGCT 1 cut(s) 1800
AfiI CCNNNNNNNGG 4 cut(s) 88, 573, 1441, 2254
AflII CTTAAG 1 cut(s) 2942
AgsI TTSAA 9 cut(s) 118, 203, 365, 764, 798, 1256, 1432, 2191, 2737
AjiI CACGTC 1 cut(s) 2491
AjnI CCWGG 4 cut(s) 2172, 2684, 2720, 2860
Alw21I GWGCWC 3 cut(s) 1105, 1594, 2336
Alw26I GTCTC 4 cut(s) 413, 927, 1277, 2645
AlwI GGATC 7 cut(s) 39, 528, 1940, 1953, 2042, 2465, 2966
AlwNI CAGNNNCTG 1 cut(s) 1271
Ama87I CYCGRG 1 cut(s) 2236
Aor51HI AGCGCT 1 cut(s) 1800
AoxI GGCC 8 cut(s) 585, 1090, 1449, 1892, 2147, 2650, 2663, 2924
ApeKI GCWGC 7 cut(s) 608, 1226, 1610, 2511, 2620, 2920, 2965
ArsI GACNNNNNNTTYG 4 cut(s) 437, 469, 905, 937
Asp700I GAANNNNTTC 3 cut(s) 1427, 1681, 1881
AspLEI GCGC 6 cut(s) 1248, 1801, 2004, 2029, 2088, 2897
AspS9I GGNCC 8 cut(s) 97, 196, 805, 1091, 1274, 2147, 2170, 2987
AsuHPI GGTGA 4 cut(s) 6, 683, 892, 2837
AvaI CYCGRG 1 cut(s) 2236
AvaII GGWCC 6 cut(s) 97, 196, 805, 1274, 2170, 2987
BalI TGGCCA 2 cut(s) 2652, 2665
BamHI GGATCC 1 cut(s) 1945
BanII GRGCYC 1 cut(s) 2249
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbsI GAAGAC 1 cut(s) 1488
Bbv12I GWGCWC 3 cut(s) 1105, 1594, 2336
BbvCI CCTCAGC 2 cut(s) 1436, 1493
BbvI GCAGC 7 cut(s) 620, 1238, 1597, 2523, 2607, 2932, 2977
BccI CCATC 6 cut(s) 504, 835, 1754, 2297, 2736, 2828
BceAI ACGGC 1 cut(s) 1989
BcgI CGANNNNNNTGC 2 cut(s) 2696, 2730
BciT130I CCWGG 4 cut(s) 2174, 2686, 2722, 2862
BciVI GTATCC 1 cut(s) 901
BcoDI GTCTC 4 cut(s) 413, 927, 1277, 2645
BfmI CTRYAG 1 cut(s) 2933
BfoI RGCGCY 3 cut(s) 1249, 1802, 2898
BfrI CTTAAG 1 cut(s) 2942
BfuAI ACCTGC 1 cut(s) 762
BfuI GTATCC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 1697
Bme1390I CCNGG 4 cut(s) 2174, 2686, 2722, 2862
Bme18I GGWCC 6 cut(s) 97, 196, 805, 1274, 2170, 2987
BmeT110I CYCGRG 1 cut(s) 2236
BmgBI CACGTC 1 cut(s) 2491
BmgT120I GGNCC 8 cut(s) 97, 196, 805, 1091, 1274, 2147, 2170, 2987
BmiI GGNNCC 7 cut(s) 1947, 2112, 2148, 2171, 2419, 2690, 2802
BmrFI CCNGG 4 cut(s) 2174, 2686, 2722, 2862
BmsI GCATC 7 cut(s) 462, 1001, 1003, 1535, 2605, 2704, 2818
BoxI GACNNNNGTC 1 cut(s) 1744
BpiI GAAGAC 1 cut(s) 1488
BplI GAGNNNNNCTC 2 cut(s) 2625, 2657
Bpu10I CCTNAGC 2 cut(s) 1436, 1493
BpuEI CTTGAG 4 cut(s) 11, 1763, 1907, 2520
BsaHI GRCGYC 1 cut(s) 735
BsaI GGTCTC 1 cut(s) 927
BsaJI CCNNGG 5 cut(s) 2142, 2591, 2647, 2684, 2692
BsaWI WCCGGW 1 cut(s) 1507
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 573, 1441, 2254
Bse1I ACTGG 5 cut(s) 82, 484, 1277, 1642, 2528
Bse3DI GCAATG 1 cut(s) 1620
BseBI CCWGG 4 cut(s) 2174, 2686, 2722, 2862
BseDI CCNNGG 5 cut(s) 2142, 2591, 2647, 2684, 2692
BseGI GGATG 5 cut(s) 1018, 1188, 1450, 2284, 2911
BseLI CCNNNNNNNGG 4 cut(s) 88, 573, 1441, 2254
BseMI GCAATG 1 cut(s) 1620
BseMII CTCAG 8 cut(s) 114, 167, 879, 1331, 1450, 1484, 1607, 1926
BseNI ACTGG 5 cut(s) 82, 484, 1277, 1642, 2528
BseRI GAGGAG 1 cut(s) 2355
BseX3I CGGCCG 1 cut(s) 2924
BseXI GCAGC 7 cut(s) 620, 1238, 1597, 2523, 2607, 2932, 2977
BsgI GTGCAG 1 cut(s) 2984
Bsh1236I CGCG 1 cut(s) 2144
Bsh1285I CGRYCG 1 cut(s) 2927
BshFI GGCC 8 cut(s) 587, 1092, 1451, 1894, 2149, 2652, 2665, 2926
BsiEI CGRYCG 1 cut(s) 2927
BsiHKAI GWGCWC 3 cut(s) 1105, 1594, 2336
BsiHKCI CYCGRG 1 cut(s) 2236
BsiSI CCGG 2 cut(s) 1508, 2559
BslFI GGGAC 1 cut(s) 2760
BslI CCNNNNNNNGG 4 cut(s) 88, 573, 1441, 2254
BsmAI GTCTC 4 cut(s) 413, 927, 1277, 2645
BsmFI GGGAC 1 cut(s) 2760
BsmI GAATGC 4 cut(s) 289, 1228, 1229, 2562
BsnI GGCC 8 cut(s) 587, 1092, 1451, 1894, 2149, 2652, 2665, 2926
Bso31I GGTCTC 1 cut(s) 927
BsoBI CYCGRG 1 cut(s) 2236
Bsp1286I GDGCHC 4 cut(s) 1105, 1594, 2249, 2336
Bsp1407I TGTACA 2 cut(s) 1542, 2777
BspANI GGCC 8 cut(s) 587, 1092, 1451, 1894, 2149, 2652, 2665, 2926
BspCNI CTCAG 8 cut(s) 115, 168, 880, 1332, 1449, 1485, 1606, 1925
BspFNI CGCG 1 cut(s) 2144
BspLI GGNNCC 7 cut(s) 1947, 2112, 2148, 2171, 2419, 2690, 2802
BspMAI CTGCAG 1 cut(s) 2937
BspMI ACCTGC 1 cut(s) 762
BspPI GGATC 7 cut(s) 39, 528, 1940, 1953, 2042, 2465, 2966
BspTI CTTAAG 1 cut(s) 2942
BspTNI GGTCTC 1 cut(s) 927
BsrDI GCAATG 1 cut(s) 1620
BsrGI TGTACA 2 cut(s) 1542, 2777
BsrI ACTGG 5 cut(s) 82, 484, 1277, 1642, 2528
BssECI CCNNGG 5 cut(s) 2142, 2591, 2647, 2684, 2692
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 2591, 2647
Bst2UI CCWGG 4 cut(s) 2174, 2686, 2722, 2862
Bst4CI ACNGT 6 cut(s) 385, 759, 861, 1135, 1212, 2195
Bst6I CTCTTC 5 cut(s) 411, 1081, 1863, 1892, 2350
BstACI GRCGYC 1 cut(s) 735
BstAFI CTTAAG 1 cut(s) 2942
BstAPI GCANNNNNTGC 2 cut(s) 1022, 1223
BstAUI TGTACA 2 cut(s) 1542, 2777
BstC8I GCNNGC 2 cut(s) 2000, 2509
BstDEI CTNAG 9 cut(s) 123, 176, 888, 941, 1340, 1436, 1493, 1593, 1912
BstDSI CCRYGG 1 cut(s) 2142
BstENI CCTNNNNNAGG 1 cut(s) 1439
BstF5I GGATG 5 cut(s) 1018, 1188, 1450, 2284, 2911
BstFNI CGCG 1 cut(s) 2144
BstH2I RGCGCY 3 cut(s) 1249, 1802, 2898
BstHHI GCGC 6 cut(s) 1248, 1801, 2004, 2029, 2088, 2897
BstMAI GTCTC 4 cut(s) 413, 927, 1277, 2645
BstMCI CGRYCG 1 cut(s) 2927
BstNI CCWGG 4 cut(s) 2174, 2686, 2722, 2862
BstNSI RCATGY 1 cut(s) 2095
BstPAI GACNNNNGTC 1 cut(s) 1744
BstSCI CCNGG 4 cut(s) 2172, 2684, 2720, 2860
BstSFI CTRYAG 1 cut(s) 2933
BstUI CGCG 1 cut(s) 2144
BstV1I GCAGC 7 cut(s) 620, 1238, 1597, 2523, 2607, 2932, 2977
BstV2I GAAGAC 1 cut(s) 1488
BstX2I RGATCY 2 cut(s) 520, 1945
BstYI RGATCY 2 cut(s) 520, 1945
BstZI CGGCCG 1 cut(s) 2924
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 8 cut(s) 587, 1092, 1451, 1894, 2149, 2652, 2665, 2926
BtgI CCRYGG 1 cut(s) 2142
BtgZI GCGATG 4 cut(s) 482, 882, 1400, 2056
BtrI CACGTC 1 cut(s) 2491
BtsCI GGATG 5 cut(s) 1018, 1188, 1450, 2284, 2911
BtsIMutI CAGTG 4 cut(s) 285, 1016, 1217, 2299
BveI ACCTGC 1 cut(s) 762
Cac8I GCNNGC 2 cut(s) 2000, 2509
CaiI CAGNNNCTG 1 cut(s) 1271
CciNI GCGGCCGC 1 cut(s) 2924
CfoI GCGC 6 cut(s) 1248, 1801, 2004, 2029, 2088, 2897
Cfr13I GGNCC 8 cut(s) 97, 196, 805, 1091, 1274, 2147, 2170, 2987
Cfr42I CCGCGG 1 cut(s) 2145
CseI GACGC 4 cut(s) 743, 2405, 2492, 2612
CsiI ACCWGGT 1 cut(s) 2720
CspCI CAANNNNNGTGG 2 cut(s) 2556, 2591
CviAII CATG 9 cut(s) 782, 904, 1240, 1523, 2063, 2092, 2464, 2755, 2969
DdeI CTNAG 9 cut(s) 123, 176, 888, 941, 1340, 1436, 1493, 1593, 1912
DraI TTTAAA 2 cut(s) 340, 1941
EaeI YGGCCR 4 cut(s) 585, 2650, 2663, 2924
EagI CGGCCG 1 cut(s) 2924
Eam1104I CTCTTC 5 cut(s) 411, 1081, 1863, 1892, 2350
EarI CTCTTC 5 cut(s) 411, 1081, 1863, 1892, 2350
EciI GGCGGA 3 cut(s) 2670, 2757, 2781
EclXI CGGCCG 1 cut(s) 2924
Eco130I CCWWGG 2 cut(s) 2591, 2647
Eco147I AGGCCT 1 cut(s) 1894
Eco24I GRGCYC 1 cut(s) 2249
Eco31I GGTCTC 1 cut(s) 927
Eco47I GGWCC 6 cut(s) 97, 196, 805, 1274, 2170, 2987
Eco47III AGCGCT 1 cut(s) 1800
Eco52I CGGCCG 1 cut(s) 2924
Eco57I CTGAAG 2 cut(s) 1442, 1873
Eco88I CYCGRG 1 cut(s) 2236
EcoNI CCTNNNNNAGG 1 cut(s) 1439
EcoO109I RGGNCCY 3 cut(s) 2147, 2170, 2987
EcoRI GAATTC 3 cut(s) 10, 974, 1753
EcoRII CCWGG 4 cut(s) 2172, 2684, 2720, 2860
EcoT14I CCWWGG 2 cut(s) 2591, 2647
EcoT22I ATGCAT 1 cut(s) 1528
EcoT38I GRGCYC 1 cut(s) 2249
ErhI CCWWGG 2 cut(s) 2591, 2647
FaeI CATG 9 cut(s) 785, 907, 1243, 1526, 2066, 2095, 2467, 2758, 2972
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 1969, 2001
FaqI GGGAC 1 cut(s) 2760
FatI CATG 9 cut(s) 781, 903, 1239, 1522, 2062, 2091, 2463, 2754, 2968
FauI CCCGC 2 cut(s) 2137, 2595
FokI GGATG 5 cut(s) 1025, 1175, 1457, 2291, 2918
FriOI GRGCYC 1 cut(s) 2249
GlaI GCGC 6 cut(s) 1247, 1800, 2003, 2028, 2087, 2896
HaeII RGCGCY 3 cut(s) 1249, 1802, 2898
HaeIII GGCC 8 cut(s) 587, 1092, 1451, 1894, 2149, 2652, 2665, 2926
HapII CCGG 2 cut(s) 1508, 2559
HgaI GACGC 4 cut(s) 743, 2405, 2492, 2612
HhaI GCGC 6 cut(s) 1248, 1801, 2004, 2029, 2088, 2897
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 9 cut(s) 785, 907, 1243, 1526, 2066, 2095, 2467, 2758, 2972
Hin6I GCGC 6 cut(s) 1246, 1799, 2002, 2027, 2086, 2895
HinP1I GCGC 6 cut(s) 1246, 1799, 2002, 2027, 2086, 2895
HincII GTYRAC 1 cut(s) 1861
HindII GTYRAC 1 cut(s) 1861
HindIII AAGCTT 2 cut(s) 227, 2852
HpaII CCGG 2 cut(s) 1508, 2559
HphI GGTGA 4 cut(s) 6, 683, 892, 2837
Hpy166II GTNNAC 4 cut(s) 1766, 1861, 1982, 2778
Hpy8I GTNNAC 4 cut(s) 1766, 1861, 1982, 2778
Hpy99I CGWCG 1 cut(s) 1337
HpyCH4III ACNGT 6 cut(s) 385, 759, 861, 1135, 1212, 2195
HpyCH4IV ACGT 4 cut(s) 954, 1069, 1355, 2490
HpyF3I CTNAG 9 cut(s) 123, 176, 888, 941, 1340, 1436, 1493, 1593, 1912
HpySE526I ACGT 4 cut(s) 954, 1069, 1355, 2490
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 9 cut(s) 785, 907, 1243, 1526, 2066, 2095, 2467, 2758, 2972
HspAI GCGC 6 cut(s) 1246, 1799, 2002, 2027, 2086, 2895
KspI CCGCGG 1 cut(s) 2145
LmnI GCTCC 6 cut(s) 768, 1346, 1510, 2110, 2383, 2453
Lsp1109I GCAGC 7 cut(s) 620, 1238, 1597, 2523, 2607, 2932, 2977
LweI GCATC 7 cut(s) 462, 1001, 1003, 1535, 2605, 2704, 2818
MabI ACCWGGT 1 cut(s) 2720
MaeII ACGT 4 cut(s) 954, 1069, 1355, 2490
MaeIII GTNAC 4 cut(s) 379, 2318, 2480, 2491
MfeI CAATTG 2 cut(s) 360, 3004
MflI RGATCY 2 cut(s) 520, 1945
MhlI GDGCHC 4 cut(s) 1105, 1594, 2249, 2336
MlsI TGGCCA 2 cut(s) 2652, 2665
MluNI TGGCCA 2 cut(s) 2652, 2665
MlyI GAGTC 5 cut(s) 415, 428, 1067, 1712, 1754
MmeI TCCRAC 2 cut(s) 174, 990
Mox20I TGGCCA 2 cut(s) 2652, 2665
Mph1103I ATGCAT 1 cut(s) 1528
MroXI GAANNNNTTC 3 cut(s) 1427, 1681, 1881
MscI TGGCCA 2 cut(s) 2652, 2665
MslI CAYNNNNRTG 3 cut(s) 246, 1238, 2614
Msp20I TGGCCA 2 cut(s) 2652, 2665
MspA1I CMGCKG 4 cut(s) 1304, 1575, 2144, 2923
MspCI CTTAAG 1 cut(s) 2942
MspI CCGG 2 cut(s) 1508, 2559
MspR9I CCNGG 4 cut(s) 2174, 2686, 2722, 2862
MunI CAATTG 2 cut(s) 360, 3004
Mva1269I GAATGC 4 cut(s) 289, 1228, 1229, 2562
MvaI CCWGG 4 cut(s) 2174, 2686, 2722, 2862
MvnI CGCG 1 cut(s) 2144
NlaIII CATG 9 cut(s) 785, 907, 1243, 1526, 2066, 2095, 2467, 2758, 2972
NlaIV GGNNCC 7 cut(s) 1947, 2112, 2148, 2171, 2419, 2690, 2802
NmuCI GTSAC 4 cut(s) 379, 2318, 2480, 2491
NotI GCGGCCGC 1 cut(s) 2924
NsiI ATGCAT 1 cut(s) 1528
NspI RCATGY 1 cut(s) 2095
PaeR7I CTCGAG 1 cut(s) 2236
PceI AGGCCT 1 cut(s) 1894
PcsI WCGNNNNNNNCGW 1 cut(s) 2319
PctI GAATGC 4 cut(s) 289, 1228, 1229, 2562
PdmI GAANNNNTTC 3 cut(s) 1427, 1681, 1881
PfeI GAWTC 7 cut(s) 166, 1076, 1141, 1169, 1428, 1599, 2423
PfoI TCCNGGA 1 cut(s) 2172
PleI GAGTC 5 cut(s) 414, 427, 1066, 1711, 1753
PpsI GAGTC 5 cut(s) 414, 427, 1066, 1711, 1753
PpuMI RGGWCCY 2 cut(s) 2170, 2987
PshAI GACNNNNGTC 1 cut(s) 1744
Psp5II RGGWCCY 2 cut(s) 2170, 2987
Psp6I CCWGG 4 cut(s) 2172, 2684, 2720, 2860
PspGI CCWGG 4 cut(s) 2172, 2684, 2720, 2860
PspN4I GGNNCC 7 cut(s) 1947, 2112, 2148, 2171, 2419, 2690, 2802
PspPI GGNCC 8 cut(s) 97, 196, 805, 1091, 1274, 2147, 2170, 2987
PspPPI RGGWCCY 2 cut(s) 2170, 2987
PsrI GAACNNNNNNTAC 2 cut(s) 1503, 1535
PstI CTGCAG 1 cut(s) 2937
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 2 cut(s) 520, 1945
PvuII CAGCTG 1 cut(s) 1304
RseI CAYNNNNRTG 3 cut(s) 246, 1238, 2614
SacII CCGCGG 1 cut(s) 2145
Sau96I GGNCC 8 cut(s) 97, 196, 805, 1091, 1274, 2147, 2170, 2987
SchI GAGTC 5 cut(s) 415, 428, 1067, 1712, 1754
ScrFI CCNGG 4 cut(s) 2174, 2686, 2722, 2862
SduI GDGCHC 4 cut(s) 1105, 1594, 2249, 2336
SexAI ACCWGGT 1 cut(s) 2720
SfaNI GCATC 7 cut(s) 462, 1001, 1003, 1535, 2605, 2704, 2818
SfcI CTRYAG 1 cut(s) 2933
Sfr274I CTCGAG 1 cut(s) 2236
Sfr303I CCGCGG 1 cut(s) 2145
SgrBI CCGCGG 1 cut(s) 2145
SinI GGWCC 6 cut(s) 97, 196, 805, 1274, 2170, 2987
SlaI CTCGAG 1 cut(s) 2236
SmiMI CAYNNNNRTG 3 cut(s) 246, 1238, 2614
SmlI CTYRAG 6 cut(s) 26, 1742, 1922, 2236, 2535, 2942
SmoI CTYRAG 6 cut(s) 26, 1742, 1922, 2236, 2535, 2942
SseBI AGGCCT 1 cut(s) 1894
SspI AATATT 1 cut(s) 2306
StuI AGGCCT 1 cut(s) 1894
StyD4I CCNGG 4 cut(s) 2172, 2684, 2720, 2860
StyI CCWWGG 2 cut(s) 2591, 2647
TaaI ACNGT 6 cut(s) 385, 759, 861, 1135, 1212, 2195
TaiI ACGT 4 cut(s) 957, 1072, 1358, 2493
TaqI TCGA 9 cut(s) 210, 863, 1099, 1281, 1365, 1844, 2237, 2330, 2436
TaqII GACCGA 1 cut(s) 2483
TatI WGTACW 4 cut(s) 1120, 1542, 2628, 2777
TauI GCSGC 4 cut(s) 587, 608, 2926, 2929
TfiI GAWTC 7 cut(s) 166, 1076, 1141, 1169, 1428, 1599, 2423
TscAI CASTG 4 cut(s) 285, 1023, 1224, 2299
TseFI GTSAC 4 cut(s) 379, 2318, 2480, 2491
TseI GCWGC 7 cut(s) 608, 1226, 1610, 2511, 2620, 2920, 2965
Tsp45I GTSAC 4 cut(s) 379, 2318, 2480, 2491
TspGWI ACGGA 3 cut(s) 412, 1880, 2193
TspRI CASTG 4 cut(s) 285, 1023, 1224, 2299
Vha464I CTTAAG 1 cut(s) 2942
VpaK11BI GGWCC 6 cut(s) 97, 196, 805, 1274, 2170, 2987
XagI CCTNNNNNAGG 1 cut(s) 1439
XbaI TCTAGA 1 cut(s) 2815
XceI RCATGY 1 cut(s) 2095
XcmI CCANNNNNNNNNTGG 2 cut(s) 595, 1693
XhoI CTCGAG 1 cut(s) 2236
XmnI GAANNNNTTC 3 cut(s) 1427, 1681, 1881
Zsp2I ATGCAT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.