Rw5G006220

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002793
Physical Location & Seq
Reverse (-)
6396797 .. 6401359
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006220.1

Sequence Viewer

Length: 3003 bp
ATGACTCTCCTTCCATGTGTGTGCTTTTTCTTGGTTGGTTTTGCTTCAATTTCAATTTCCAACATTGGTAGATTACTGGCTTTCACTAATGGCCTGAACAAAGTAGACATAATGGCTAAGCCACAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGATTTGTACCAGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTGCTATAAGCTTATAGATGAATTTCGTAAGAGGATAAAGAAAAGTTCAGCAGCGCCTCAAGCTGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCAAAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAAAGCCGAAATTGGGCAGTTGCAGCCACTCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTGTTGGCGATGTATATTGGTGATGAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTGCAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACACTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTTGCATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTACATTGGAGTCATATCGACATATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCGTACAGAATCTAGTTAAGATCCCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGTTGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCAAGACATGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGACACGTATGGGGTTGATGAGAAAAGCAAAAGAGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCAATGTCAGCAAGTGTCCAGAAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATAGACAATTATGAAGGTGGAATGGATTGTCGTTCTCAGCGTATCATTGATGCTGCAATTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCCGGACAGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAGAGTATCTAGTCAGAAGATTGACAAAGCACCAGAGGAATGTGAGAAACCGATGAGTGATGGGGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGCTTTAAAGGACCCAACCTCTCCAAAACATTCAATGGATCAAAAGGTACATTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCCATTTCCGGAAACTCCAATCAGCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCAAACATGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCCATAAGTGACAATGAAGATTCTTTCAGCCATCACGAATTTACAAGCACCTTGTCCTCTCCGTCTTCTTATACTGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATGAGGGGATCAGCACGGCAGATACTGCGTTTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATCAACGGGGAAATCTTGCGGCTTCTAATCCGGCAATGCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGGGAGATCCAACTGTGAGAGGTCCATATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

1000

Amino Acids

113.38

Weight (kDa)

6.01

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 102 - 207 2.3e-15 Telomerase activating protein Est1
EST1_DNA_bind PF10373 219 - 525 2.1e-55 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 105
AccIII TCCGGA 2 cut(s) 1771, 2191
AciI CCGC 1 cut(s) 2786
AcoI YGGCCR 1 cut(s) 689
AcsI RAATTY 8 cut(s) 347, 432, 448, 684, 973, 1466, 1733, 2393
AcuI CTGAAG 3 cut(s) 447, 2319, 2427
AfaI GTAC 7 cut(s) 236, 1182, 1197, 1336, 2028, 2660, 2735
AfeI AGCGCT 1 cut(s) 2209
AfiI CCNNNNNNNGG 3 cut(s) 646, 698, 2323
AflII CTTAAG 1 cut(s) 2939
AflIII ACRYGT 2 cut(s) 1413, 2136
AjnI CCWGG 4 cut(s) 1167, 2830, 2848, 2961
AjuI GAANNNNNNNTTGG 2 cut(s) 2331, 2363
Alw21I GWGCWC 1 cut(s) 1167
Alw26I GTCTC 2 cut(s) 987, 1793
AlwNI CAGNNNCTG 1 cut(s) 2510
Ama87I CYCGRG 1 cut(s) 587
Aor13HI TCCGGA 2 cut(s) 1771, 2191
Aor51HI AGCGCT 1 cut(s) 2209
ApaI GGGCCC 1 cut(s) 2697
ApeKI GCWGC 9 cut(s) 377, 656, 1252, 1664, 2149, 2152, 2155, 2541, 2917
ApoI RAATTY 8 cut(s) 347, 432, 448, 684, 973, 1466, 1733, 2393
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
Asp700I GAANNNNTTC 4 cut(s) 432, 1926, 2635, 2649
AspLEI GCGC 4 cut(s) 382, 2049, 2133, 2210
AspS9I GGNCC 7 cut(s) 1151, 1990, 2213, 2570, 2693, 2694, 2984
AsuC2I CCSGG 1 cut(s) 2081
AsuHPI GGTGA 3 cut(s) 755, 814, 1475
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 2 cut(s) 1990, 2984
AxyI CCTNAGG 1 cut(s) 804
BaeGI GKGCMC 2 cut(s) 190, 2697
BalI TGGCCA 1 cut(s) 691
BanII GRGCYC 3 cut(s) 2318, 2475, 2697
BbsI GAAGAC 3 cut(s) 1384, 1542, 2412
Bbv12I GWGCWC 1 cut(s) 1167
BbvCI CCTCAGC 1 cut(s) 1547
BbvI GCAGC 9 cut(s) 389, 668, 1264, 1651, 2161, 2164, 2167, 2553, 2929
BccI CCATC 6 cut(s) 576, 907, 1075, 1877, 2394, 2597
BceAI ACGGC 2 cut(s) 2034, 2517
BciT130I CCWGG 4 cut(s) 1169, 2832, 2850, 2963
BciVI GTATCC 2 cut(s) 961, 2838
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 1 cut(s) 2081
BcoDI GTCTC 2 cut(s) 987, 1793
BfaI CTAG 8 cut(s) 600, 1088, 1205, 1332, 1586, 1833, 1947, 2843
BfmI CTRYAG 1 cut(s) 502
BfoI RGCGCY 2 cut(s) 383, 2211
BfrI CTTAAG 1 cut(s) 2939
BfuI GTATCC 2 cut(s) 961, 2838
BlpI GCTNAGC 1 cut(s) 117
Bme1390I CCNGG 5 cut(s) 1169, 2081, 2832, 2850, 2963
Bme18I GGWCC 2 cut(s) 1990, 2984
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 7 cut(s) 1151, 1990, 2213, 2570, 2693, 2694, 2984
BmiI GGNNCC 2 cut(s) 1992, 2695
BmrFI CCNGG 5 cut(s) 1169, 2081, 2832, 2850, 2963
BmrI ACTGGG 1 cut(s) 2709
BmsI GCATC 5 cut(s) 534, 1061, 1063, 1651, 2692
BmuI ACTGGG 1 cut(s) 2709
BpiI GAAGAC 3 cut(s) 1384, 1542, 2412
BpmI CTGGAG 2 cut(s) 1151, 2945
Bpu10I CCTNAGC 1 cut(s) 1547
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 3 cut(s) 369, 2550, 2699
BpuMI CCSGG 1 cut(s) 2081
BsaAI YACGTR 1 cut(s) 1416
BsaBI GATNNNNATC 1 cut(s) 1190
BsaI GGTCTC 1 cut(s) 987
BsaJI CCNNGG 6 cut(s) 526, 586, 810, 2722, 2750, 2962
BsaWI WCCGGW 2 cut(s) 1771, 2191
Bsc4I CCNNNNNNNGG 3 cut(s) 646, 698, 2323
Bse1I ACTGG 4 cut(s) 81, 1562, 1964, 2715
Bse21I CCTNAGG 1 cut(s) 804
Bse3DI GCAATG 2 cut(s) 946, 2808
Bse8I GATNNNNATC 1 cut(s) 1190
BseAI TCCGGA 2 cut(s) 1771, 2191
BseBI CCWGG 4 cut(s) 1169, 2832, 2850, 2963
BseDI CCNNGG 6 cut(s) 526, 586, 810, 2722, 2750, 2962
BseGI GGATG 4 cut(s) 267, 895, 1078, 2707
BseJI GATNNNNATC 1 cut(s) 1190
BseLI CCNNNNNNNGG 3 cut(s) 646, 698, 2323
BseMI GCAATG 2 cut(s) 946, 2808
BseMII CTCAG 4 cut(s) 795, 1538, 1661, 2099
BseNI ACTGG 4 cut(s) 81, 1562, 1964, 2715
BseSI GKGCMC 2 cut(s) 190, 2697
BseXI GCAGC 9 cut(s) 389, 668, 1264, 1651, 2161, 2164, 2167, 2553, 2929
BsgI GTGCAG 1 cut(s) 2607
BsiHKAI GWGCWC 1 cut(s) 1167
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 4 cut(s) 1772, 2081, 2192, 2798
BslFI GGGAC 3 cut(s) 1317, 2299, 2571
BslI CCNNNNNNNGG 3 cut(s) 646, 698, 2323
BsmAI GTCTC 2 cut(s) 987, 1793
BsmFI GGGAC 3 cut(s) 1317, 2299, 2571
BsmI GAATGC 1 cut(s) 476
Bso31I GGTCTC 1 cut(s) 987
BsoBI CYCGRG 1 cut(s) 587
Bsp120I GGGCCC 1 cut(s) 2693
Bsp1286I GDGCHC 5 cut(s) 190, 1167, 2318, 2475, 2697
Bsp13I TCCGGA 2 cut(s) 1771, 2191
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 1 cut(s) 2786
BspCNI CTCAG 4 cut(s) 796, 1539, 1660, 2098
BspEI TCCGGA 2 cut(s) 1771, 2191
BspLI GGNNCC 2 cut(s) 1992, 2695
BspTI CTTAAG 1 cut(s) 2939
BspTNI GGTCTC 1 cut(s) 987
BsrDI GCAATG 2 cut(s) 946, 2808
BsrI ACTGG 4 cut(s) 81, 1562, 1964, 2715
BssECI CCNNGG 6 cut(s) 526, 586, 810, 2722, 2750, 2962
BssT1I CCWWGG 4 cut(s) 526, 810, 2722, 2750
Bst2UI CCWGG 4 cut(s) 1169, 2832, 2850, 2963
Bst4CI ACNGT 6 cut(s) 126, 770, 831, 1272, 2738, 2977
Bst6I CTCTTC 2 cut(s) 483, 1908
BstAFI CTTAAG 1 cut(s) 2939
BstAPI GCANNNNNTGC 1 cut(s) 2510
BstBAI YACGTR 1 cut(s) 1416
BstC8I GCNNGC 5 cut(s) 166, 756, 1329, 2212, 2539
BstDEI CTNAG 9 cut(s) 117, 804, 1001, 1547, 1647, 1895, 2085, 2225, 2906
BstEII GGTNACC 1 cut(s) 2861
BstF5I GGATG 4 cut(s) 267, 895, 1078, 2707
BstH2I RGCGCY 2 cut(s) 383, 2211
BstHHI GCGC 4 cut(s) 382, 2049, 2133, 2210
BstMAI GTCTC 2 cut(s) 987, 1793
BstMWI GCNNNNNNNGC 9 cut(s) 386, 656, 728, 1082, 1261, 1565, 2155, 2256, 2510
BstNI CCWGG 4 cut(s) 1169, 2832, 2850, 2963
BstNSI RCATGY 3 cut(s) 1350, 1580, 2140
BstPI GGTNACC 1 cut(s) 2861
BstSCI CCNGG 5 cut(s) 1167, 2079, 2830, 2848, 2961
BstSFI CTRYAG 1 cut(s) 502
BstSLI GKGCMC 2 cut(s) 190, 2697
BstV1I GCAGC 9 cut(s) 389, 668, 1264, 1651, 2161, 2164, 2167, 2553, 2929
BstV2I GAAGAC 3 cut(s) 1384, 1542, 2412
BstX2I RGATCY 4 cut(s) 592, 1212, 2845, 2968
BstYI RGATCY 4 cut(s) 592, 1212, 2845, 2968
Bsu36I CCTNAGG 1 cut(s) 804
BsuI GTATCC 2 cut(s) 961, 2838
BtgZI GCGATG 2 cut(s) 554, 746
BtsCI GGATG 4 cut(s) 267, 895, 1078, 2707
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 3 cut(s) 188, 766, 919
Cac8I GCNNGC 5 cut(s) 166, 756, 1329, 2212, 2539
CaiI CAGNNNCTG 1 cut(s) 2510
CfoI GCGC 4 cut(s) 382, 2049, 2133, 2210
Cfr13I GGNCC 7 cut(s) 1151, 1990, 2213, 2570, 2693, 2694, 2984
CseI GACGC 1 cut(s) 1314
Csp6I GTAC 7 cut(s) 235, 1181, 1196, 1335, 2027, 2659, 2734
CviQI GTAC 7 cut(s) 235, 1181, 1196, 1335, 2027, 2659, 2734
DdeI CTNAG 9 cut(s) 117, 804, 1001, 1547, 1647, 1895, 2085, 2225, 2906
DraI TTTAAA 1 cut(s) 1986
EaeI YGGCCR 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 483, 1908
EarI CTCTTC 2 cut(s) 483, 1908
Eco130I CCWWGG 4 cut(s) 526, 810, 2722, 2750
Eco147I AGGCCT 1 cut(s) 1939
Eco24I GRGCYC 3 cut(s) 2318, 2475, 2697
Eco31I GGTCTC 1 cut(s) 987
Eco32I GATATC 1 cut(s) 1769
Eco47I GGWCC 2 cut(s) 1990, 2984
Eco47III AGCGCT 1 cut(s) 2209
Eco57I CTGAAG 3 cut(s) 447, 2319, 2427
Eco81I CCTNAGG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 587
Eco91I GGTNACC 1 cut(s) 2861
EcoO109I RGGNCCY 2 cut(s) 1151, 1990
EcoO65I GGTNACC 1 cut(s) 2861
EcoRI GAATTC 1 cut(s) 432
EcoRII CCWGG 4 cut(s) 1167, 2830, 2848, 2961
EcoRV GATATC 1 cut(s) 1769
EcoT14I CCWWGG 4 cut(s) 526, 810, 2722, 2750
EcoT22I ATGCAT 3 cut(s) 1078, 1582, 2808
EcoT38I GRGCYC 3 cut(s) 2318, 2475, 2697
ErhI CCWWGG 4 cut(s) 526, 810, 2722, 2750
FalI AAGNNNNNCTT 2 cut(s) 1914, 1946
FaqI GGGAC 3 cut(s) 1317, 2299, 2571
FauNDI CATATG 1 cut(s) 1131
FbaI TGATCA 1 cut(s) 990
FblI GTMKAC 1 cut(s) 105
FokI GGATG 4 cut(s) 254, 882, 1085, 2694
FriOI GRGCYC 3 cut(s) 2318, 2475, 2697
FspBI CTAG 8 cut(s) 600, 1088, 1205, 1332, 1586, 1833, 1947, 2843
GlaI GCGC 4 cut(s) 381, 2048, 2132, 2209
GsuI CTGGAG 2 cut(s) 1151, 2945
HaeII RGCGCY 2 cut(s) 383, 2211
HapII CCGG 4 cut(s) 1772, 2081, 2192, 2798
HgaI GACGC 1 cut(s) 1314
HhaI GCGC 4 cut(s) 382, 2049, 2133, 2210
Hin6I GCGC 4 cut(s) 380, 2047, 2131, 2208
HinP1I GCGC 4 cut(s) 380, 2047, 2131, 2208
HindIII AAGCTT 2 cut(s) 323, 335
HpaII CCGG 4 cut(s) 1772, 2081, 2192, 2798
HphI GGTGA 3 cut(s) 755, 814, 1475
Hpy166II GTNNAC 3 cut(s) 106, 2294, 2608
Hpy8I GTNNAC 3 cut(s) 106, 2294, 2608
HpyAV CCTTC 7 cut(s) 20, 398, 422, 1364, 1511, 1619, 2451
HpyCH4III ACNGT 6 cut(s) 126, 770, 831, 1272, 2738, 2977
HpyCH4IV ACGT 3 cut(s) 1415, 1951, 2057
HpyF10VI GCNNNNNNNGC 9 cut(s) 386, 656, 728, 1082, 1261, 1565, 2155, 2256, 2510
HpyF3I CTNAG 9 cut(s) 117, 804, 1001, 1547, 1647, 1895, 2085, 2225, 2906
HpySE526I ACGT 3 cut(s) 1415, 1951, 2057
HspAI GCGC 4 cut(s) 380, 2047, 2131, 2208
Kpn2I TCCGGA 2 cut(s) 1771, 2191
Ksp22I TGATCA 1 cut(s) 990
LmnI GCTCC 3 cut(s) 1170, 1261, 2437
Lsp1109I GCAGC 9 cut(s) 389, 668, 1264, 1651, 2161, 2164, 2167, 2553, 2929
LweI GCATC 5 cut(s) 534, 1061, 1063, 1651, 2692
MaeI CTAG 8 cut(s) 600, 1088, 1205, 1332, 1586, 1833, 1947, 2843
MaeII ACGT 3 cut(s) 1415, 1951, 2057
MaeIII GTNAC 6 cut(s) 2058, 2164, 2363, 2717, 2861, 2896
MfeI CAATTG 2 cut(s) 1487, 2998
MflI RGATCY 4 cut(s) 592, 1212, 2845, 2968
MhlI GDGCHC 5 cut(s) 190, 1167, 2318, 2475, 2697
MlsI TGGCCA 1 cut(s) 691
MluNI TGGCCA 1 cut(s) 691
MlyI GAGTC 7 cut(s) 500, 1127, 1223, 1766, 1793, 2113, 2810
MmeI TCCRAC 3 cut(s) 84, 1050, 2996
Mox20I TGGCCA 1 cut(s) 691
Mph1103I ATGCAT 3 cut(s) 1078, 1582, 2808
MroI TCCGGA 2 cut(s) 1771, 2191
MroXI GAANNNNTTC 4 cut(s) 432, 1926, 2635, 2649
MscI TGGCCA 1 cut(s) 691
MseI TTAA 9 cut(s) 410, 789, 885, 1016, 1209, 1671, 1985, 2484, 2940
MslI CAYNNNNRTG 7 cut(s) 19, 219, 1298, 2034, 2644, 2688, 2811
Msp20I TGGCCA 1 cut(s) 691
MspA1I CMGCKG 3 cut(s) 181, 289, 1364
MspCI CTTAAG 1 cut(s) 2939
MspI CCGG 4 cut(s) 1772, 2081, 2192, 2798
MspR9I CCNGG 5 cut(s) 1169, 2081, 2832, 2850, 2963
MunI CAATTG 2 cut(s) 1487, 2998
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 4 cut(s) 1169, 2832, 2850, 2963
MwoI GCNNNNNNNGC 9 cut(s) 386, 656, 728, 1082, 1261, 1565, 2155, 2256, 2510
NciI CCSGG 1 cut(s) 2081
NdeI CATATG 1 cut(s) 1131
NlaIV GGNNCC 2 cut(s) 1992, 2695
NmuCI GTSAC 4 cut(s) 2058, 2164, 2363, 2896
NsiI ATGCAT 3 cut(s) 1078, 1582, 2808
NspI RCATGY 3 cut(s) 1350, 1580, 2140
PceI AGGCCT 1 cut(s) 1939
PciI ACATGT 1 cut(s) 2136
PcsI WCGNNNNNNNCGW 1 cut(s) 1648
PctI GAATGC 1 cut(s) 476
PdmI GAANNNNTTC 4 cut(s) 432, 1926, 2635, 2649
PfeI GAWTC 6 cut(s) 175, 1136, 1201, 1694, 2339, 2375
PflFI GACNNNGTC 1 cut(s) 1756
PflMI CCANNNNNTGG 1 cut(s) 698
PleI GAGTC 7 cut(s) 499, 1126, 1223, 1765, 1792, 2113, 2810
PpsI GAGTC 7 cut(s) 499, 1126, 1223, 1765, 1792, 2113, 2810
Ppu21I YACGTR 1 cut(s) 1416
PpuMI RGGWCCY 1 cut(s) 1990
PscI ACATGT 1 cut(s) 2136
Psp5II RGGWCCY 1 cut(s) 1990
Psp6I CCWGG 4 cut(s) 1167, 2830, 2848, 2961
PspEI GGTNACC 1 cut(s) 2861
PspGI CCWGG 4 cut(s) 1167, 2830, 2848, 2961
PspN4I GGNNCC 2 cut(s) 1992, 2695
PspOMI GGGCCC 1 cut(s) 2693
PspPI GGNCC 7 cut(s) 1151, 1990, 2213, 2570, 2693, 2694, 2984
PspPPI RGGWCCY 1 cut(s) 1990
PstNI CAGNNNCTG 1 cut(s) 2510
PsuI RGATCY 4 cut(s) 592, 1212, 2845, 2968
PsyI GACNNNGTC 1 cut(s) 1756
PvuII CAGCTG 3 cut(s) 181, 289, 1364
RsaI GTAC 7 cut(s) 236, 1182, 1197, 1336, 2028, 2660, 2735
RsaNI GTAC 7 cut(s) 235, 1181, 1196, 1335, 2027, 2659, 2734
RseI CAYNNNNRTG 7 cut(s) 19, 219, 1298, 2034, 2644, 2688, 2811
SaqAI TTAA 9 cut(s) 410, 789, 885, 1016, 1209, 1671, 1985, 2484, 2940
Sau96I GGNCC 7 cut(s) 1151, 1990, 2213, 2570, 2693, 2694, 2984
SchI GAGTC 7 cut(s) 500, 1127, 1223, 1766, 1793, 2113, 2810
ScrFI CCNGG 5 cut(s) 1169, 2081, 2832, 2850, 2963
SduI GDGCHC 5 cut(s) 190, 1167, 2318, 2475, 2697
SfaNI GCATC 5 cut(s) 534, 1061, 1063, 1651, 2692
SfcI CTRYAG 1 cut(s) 502
SinI GGWCC 2 cut(s) 1990, 2984
SmiMI CAYNNNNRTG 7 cut(s) 19, 219, 1298, 2034, 2644, 2688, 2811
SmlI CTYRAG 4 cut(s) 384, 2565, 2678, 2939
SmoI CTYRAG 4 cut(s) 384, 2565, 2678, 2939
SseBI AGGCCT 1 cut(s) 1939
SsiI CCGC 1 cut(s) 2786
SspMI CTAG 8 cut(s) 600, 1088, 1205, 1332, 1586, 1833, 1947, 2843
StuI AGGCCT 1 cut(s) 1939
StyD4I CCNGG 5 cut(s) 1167, 2079, 2830, 2848, 2961
StyI CCWWGG 4 cut(s) 526, 810, 2722, 2750
TaaI ACNGT 6 cut(s) 126, 770, 831, 1272, 2738, 2977
TaiI ACGT 3 cut(s) 1418, 1954, 2060
TaqI TCGA 4 cut(s) 1126, 1159, 1232, 2819
TatI WGTACW 3 cut(s) 1180, 1334, 2658
TauI GCSGC 1 cut(s) 2789
TfiI GAWTC 6 cut(s) 175, 1136, 1201, 1694, 2339, 2375
Tru1I TTAA 9 cut(s) 410, 789, 885, 1016, 1209, 1671, 1985, 2484, 2940
Tru9I TTAA 9 cut(s) 410, 789, 885, 1016, 1209, 1671, 1985, 2484, 2940
TscAI CASTG 3 cut(s) 195, 773, 919
TseFI GTSAC 4 cut(s) 2058, 2164, 2363, 2896
TseI GCWGC 9 cut(s) 377, 656, 1252, 1664, 2149, 2152, 2155, 2541, 2917
Tsp45I GTSAC 4 cut(s) 2058, 2164, 2363, 2896
TspGWI ACGGA 1 cut(s) 2406
TspRI CASTG 3 cut(s) 195, 773, 919
Tth111I GACNNNGTC 1 cut(s) 1756
Van91I CCANNNNNTGG 1 cut(s) 698
Vha464I CTTAAG 1 cut(s) 2939
VpaK11BI GGWCC 2 cut(s) 1990, 2984
XapI RAATTY 8 cut(s) 347, 432, 448, 684, 973, 1466, 1733, 2393
XbaI TCTAGA 1 cut(s) 2842
XceI RCATGY 3 cut(s) 1350, 1580, 2140
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XmiI GTMKAC 1 cut(s) 105
XmnI GAANNNNTTC 4 cut(s) 432, 1926, 2635, 2649
XspI CTAG 8 cut(s) 600, 1088, 1205, 1332, 1586, 1833, 1947, 2843
Zsp2I ATGCAT 3 cut(s) 1078, 1582, 2808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.