RchiOBHm_Chr5g0008711

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002793
Physical Location & Seq
Forward (+)
5631244 .. 5636321
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28964

Sequence Viewer

Length: 2904 bp
ATGGCTAAGCCACAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGACTTGTACCGGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGGATAAAGAAAAGTTCAGCATTGCCTCAACATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATTCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCATAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAAAGCCGAAATTGGTCAGTTGCAGCCACTCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTATTGGCGATGTATATTGGTGATCAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTACAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACACTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTCGCATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTACATTGGAGTCATATCAACGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCATACAGAATCTAGTTAAGATCTCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGTTGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCAAGACATGCCCCCTTCTACCAGCTGTGCTTGTGTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCAATGTCGCTGAGGCCAAGTGTCCAGAAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATAGACAATTATGAAGGTGGAATGGATTGTCGTTCTCAGCGTATCATTGATGCCGCAATTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCCGGACAGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAGAGTGTCTAGTCAGAAGATTGACAAAGCACCAGAGGAATGTGAGAAACCGATGAGTGATGGGGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGCTTTAAAGGACCCAACCTCTCCAAAACATTCATTGGATCAAAAGGTACCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGACCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCCATTTCCGGAAACTCCAATCACCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCACACATGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTGTCCATAAGTGGCAATGAAGATTCTTTCAGCCATCACGAATTTGCAAGCACCTTATCCTCTCCGTCTTCTTATACTGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATGAGGGGATCAGCACGGCAGATAATGCTTTTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATCAACGGGGAAATCTTGTGGCTTCTAATCCGGCAATGTCGAGTCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGAGAGATCCAACTGTGAGAGGTCCATATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

967

Amino Acids

109.96

Weight (kDa)

5.96

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 65 - 170 3.6e-15 Telomerase activating protein Est1
EST1_DNA_bind PF10373 182 - 490 3.5e-56 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1921
AccB1I GGYRCC 1 cut(s) 1921
AccB7I CCANNNNNTGG 1 cut(s) 587
AccIII TCCGGA 2 cut(s) 1666, 2086
AciI CCGC 2 cut(s) 1560, 2103
AclWI GGATC 8 cut(s) 76, 489, 927, 1920, 2203, 2396, 2741, 2864
AcoI YGGCCR 1 cut(s) 578
AcsI RAATTY 9 cut(s) 236, 321, 337, 359, 573, 862, 1355, 1628, 2288
AcuI CTGAAG 3 cut(s) 336, 2214, 2322
AfaI GTAC 6 cut(s) 125, 1071, 1225, 1923, 2555, 2630
AfiI CCNNNNNNNGG 2 cut(s) 587, 2218
AflII CTTAAG 1 cut(s) 2840
AflIII ACRYGT 1 cut(s) 2031
AgsI TTSAA 8 cut(s) 19, 188, 725, 759, 1205, 1381, 1715, 2662
AjnI CCWGG 3 cut(s) 1056, 2731, 2749
AjuI GAANNNNNNNTTGG 2 cut(s) 2226, 2258
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 2 cut(s) 876, 1688
AlwI GGATC 8 cut(s) 76, 489, 927, 1920, 2203, 2396, 2741, 2864
Ama87I CYCGRG 1 cut(s) 476
Aor13HI TCCGGA 2 cut(s) 1666, 2086
ApaI GGGCCC 1 cut(s) 2592
ApeKI GCWGC 7 cut(s) 545, 1141, 2044, 2047, 2050, 2436, 2818
ApoI RAATTY 9 cut(s) 236, 321, 337, 359, 573, 862, 1355, 1628, 2288
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
Asp700I GAANNNNTTC 4 cut(s) 321, 1821, 2530, 2544
Asp718I GGTACC 1 cut(s) 1921
AspLEI GCGC 2 cut(s) 1944, 2028
AspS9I GGNCC 7 cut(s) 1040, 1885, 2108, 2465, 2588, 2589, 2885
AsuC2I CCSGG 1 cut(s) 1976
AsuHPI GGTGA 4 cut(s) 644, 703, 1364, 2092
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 2 cut(s) 1885, 2885
AxyI CCTNAGG 1 cut(s) 693
BaeGI GKGCMC 2 cut(s) 79, 2592
BalI TGGCCA 1 cut(s) 580
BanI GGYRCC 1 cut(s) 1921
BanII GRGCYC 3 cut(s) 2213, 2370, 2592
BbsI GAAGAC 3 cut(s) 1273, 1437, 2307
Bbv12I GWGCWC 1 cut(s) 1056
BbvCI CCTCAGC 2 cut(s) 1395, 1442
BbvI GCAGC 7 cut(s) 557, 1153, 2056, 2059, 2062, 2448, 2830
BccI CCATC 6 cut(s) 465, 796, 964, 1772, 2289, 2492
BceAI ACGGC 2 cut(s) 1929, 2412
BciT130I CCWGG 3 cut(s) 1058, 2733, 2751
BciVI GTATCC 2 cut(s) 850, 2739
BclI TGATCA 2 cut(s) 634, 879
BcnI CCSGG 1 cut(s) 1976
BcoDI GTCTC 2 cut(s) 876, 1688
BfaI CTAG 8 cut(s) 489, 977, 1094, 1221, 1481, 1728, 1842, 2744
BfmI CTRYAG 1 cut(s) 391
BfrI CTTAAG 1 cut(s) 2840
BfuI GTATCC 2 cut(s) 850, 2739
BglII AGATCT 1 cut(s) 1101
BisI GCNGC 8 cut(s) 546, 1142, 1560, 2045, 2048, 2051, 2437, 2819
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 8 cut(s) 547, 1143, 1561, 2046, 2049, 2052, 2438, 2820
Bme1390I CCNGG 4 cut(s) 1058, 1976, 2733, 2751
Bme18I GGWCC 2 cut(s) 1885, 2885
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 7 cut(s) 1040, 1885, 2108, 2465, 2588, 2589, 2885
BmiI GGNNCC 3 cut(s) 1887, 1923, 2590
BmrFI CCNGG 4 cut(s) 1058, 1976, 2733, 2751
BmrI ACTGGG 1 cut(s) 2604
BmsI GCATC 5 cut(s) 423, 950, 952, 1546, 2587
BmuI ACTGGG 1 cut(s) 2604
BpiI GAAGAC 3 cut(s) 1273, 1437, 2307
BpmI CTGGAG 2 cut(s) 1040, 2846
Bpu10I CCTNAGC 2 cut(s) 1395, 1442
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 2445, 2594
BpuMI CCSGG 1 cut(s) 1976
BsaBI GATNNNNATC 1 cut(s) 1079
BsaI GGTCTC 1 cut(s) 876
BsaJI CCNNGG 5 cut(s) 415, 475, 699, 2617, 2645
BsaWI WCCGGW 3 cut(s) 126, 1666, 2086
Bsc4I CCNNNNNNNGG 2 cut(s) 587, 2218
Bse1I ACTGG 3 cut(s) 1457, 1859, 2610
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 4 cut(s) 266, 835, 2269, 2703
Bse8I GATNNNNATC 1 cut(s) 1079
BseAI TCCGGA 2 cut(s) 1666, 2086
BseBI CCWGG 3 cut(s) 1058, 2733, 2751
BseDI CCNNGG 5 cut(s) 415, 475, 699, 2617, 2645
BseGI GGATG 4 cut(s) 156, 784, 967, 2602
BseJI GATNNNNATC 1 cut(s) 1079
BseLI CCNNNNNNNGG 2 cut(s) 587, 2218
BseMI GCAATG 4 cut(s) 266, 835, 2269, 2703
BseMII CTCAG 6 cut(s) 684, 1119, 1386, 1433, 1556, 1994
BseNI ACTGG 3 cut(s) 1457, 1859, 2610
BseSI GKGCMC 2 cut(s) 79, 2592
BseXI GCAGC 7 cut(s) 557, 1153, 2056, 2059, 2062, 2448, 2830
BsgI GTGCAG 1 cut(s) 2502
BshNI GGYRCC 1 cut(s) 1921
BsiHKAI GWGCWC 1 cut(s) 1056
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 5 cut(s) 127, 1667, 1976, 2087, 2693
BslFI GGGAC 3 cut(s) 1206, 2194, 2466
BslI CCNNNNNNNGG 2 cut(s) 587, 2218
BsmAI GTCTC 2 cut(s) 876, 1688
BsmFI GGGAC 3 cut(s) 1206, 2194, 2466
Bso31I GGTCTC 1 cut(s) 876
BsoBI CYCGRG 1 cut(s) 476
Bsp120I GGGCCC 1 cut(s) 2588
Bsp1286I GDGCHC 5 cut(s) 79, 1056, 2213, 2370, 2592
Bsp13I TCCGGA 2 cut(s) 1666, 2086
Bsp1720I GCTNAGC 1 cut(s) 6
BspACI CCGC 2 cut(s) 1560, 2103
BspCNI CTCAG 6 cut(s) 685, 1118, 1387, 1434, 1555, 1993
BspEI TCCGGA 2 cut(s) 1666, 2086
BspLI GGNNCC 3 cut(s) 1887, 1923, 2590
BspPI GGATC 8 cut(s) 76, 489, 927, 1920, 2203, 2396, 2741, 2864
BspT107I GGYRCC 1 cut(s) 1921
BspTI CTTAAG 1 cut(s) 2840
BspTNI GGTCTC 1 cut(s) 876
BsrDI GCAATG 4 cut(s) 266, 835, 2269, 2703
BsrI ACTGG 3 cut(s) 1457, 1859, 2610
BssECI CCNNGG 5 cut(s) 415, 475, 699, 2617, 2645
BssT1I CCWWGG 4 cut(s) 415, 699, 2617, 2645
Bst2UI CCWGG 3 cut(s) 1058, 2733, 2751
Bst4CI ACNGT 6 cut(s) 15, 659, 720, 1161, 2633, 2878
Bst6I CTCTTC 2 cut(s) 372, 1803
BstAFI CTTAAG 1 cut(s) 2840
BstAPI GCANNNNNTGC 1 cut(s) 2405
BstC8I GCNNGC 6 cut(s) 55, 645, 1218, 2107, 2296, 2434
BstEII GGTNACC 1 cut(s) 2762
BstF5I GGATG 4 cut(s) 156, 784, 967, 2602
BstHHI GCGC 2 cut(s) 1944, 2028
BstMAI GTCTC 2 cut(s) 876, 1688
BstMWI GCNNNNNNNGC 7 cut(s) 617, 971, 1150, 1460, 2050, 2151, 2405
BstNI CCWGG 3 cut(s) 1058, 2733, 2751
BstNSI RCATGY 3 cut(s) 1239, 1475, 2035
BstPI GGTNACC 1 cut(s) 2762
BstSCI CCNGG 4 cut(s) 1056, 1974, 2731, 2749
BstSFI CTRYAG 1 cut(s) 391
BstSLI GKGCMC 2 cut(s) 79, 2592
BstV1I GCAGC 7 cut(s) 557, 1153, 2056, 2059, 2062, 2448, 2830
BstV2I GAAGAC 3 cut(s) 1273, 1437, 2307
BstX2I RGATCY 4 cut(s) 481, 1101, 2746, 2869
BstYI RGATCY 4 cut(s) 481, 1101, 2746, 2869
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 2 cut(s) 850, 2739
BtgZI GCGATG 2 cut(s) 443, 635
BtsCI GGATG 4 cut(s) 156, 784, 967, 2602
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 3 cut(s) 77, 655, 808
Cac8I GCNNGC 6 cut(s) 55, 645, 1218, 2107, 2296, 2434
CfoI GCGC 2 cut(s) 1944, 2028
Cfr13I GGNCC 7 cut(s) 1040, 1885, 2108, 2465, 2588, 2589, 2885
CseI GACGC 1 cut(s) 1203
Csp6I GTAC 6 cut(s) 124, 1070, 1224, 1922, 2554, 2629
CviQI GTAC 6 cut(s) 124, 1070, 1224, 1922, 2554, 2629
DraI TTTAAA 1 cut(s) 1881
EaeI YGGCCR 1 cut(s) 578
Eam1104I CTCTTC 2 cut(s) 372, 1803
EarI CTCTTC 2 cut(s) 372, 1803
Eco130I CCWWGG 4 cut(s) 415, 699, 2617, 2645
Eco147I AGGCCT 1 cut(s) 1834
Eco24I GRGCYC 3 cut(s) 2213, 2370, 2592
Eco31I GGTCTC 1 cut(s) 876
Eco32I GATATC 1 cut(s) 1664
Eco47I GGWCC 2 cut(s) 1885, 2885
Eco57I CTGAAG 3 cut(s) 336, 2214, 2322
Eco81I CCTNAGG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 476
Eco91I GGTNACC 1 cut(s) 2762
EcoO109I RGGNCCY 2 cut(s) 1040, 1885
EcoO65I GGTNACC 1 cut(s) 2762
EcoRI GAATTC 2 cut(s) 321, 359
EcoRII CCWGG 3 cut(s) 1056, 2731, 2749
EcoRV GATATC 1 cut(s) 1664
EcoT14I CCWWGG 4 cut(s) 415, 699, 2617, 2645
EcoT22I ATGCAT 2 cut(s) 967, 1477
EcoT38I GRGCYC 3 cut(s) 2213, 2370, 2592
ErhI CCWWGG 4 cut(s) 415, 699, 2617, 2645
FalI AAGNNNNNCTT 2 cut(s) 1809, 1841
FaqI GGGAC 3 cut(s) 1206, 2194, 2466
FbaI TGATCA 2 cut(s) 634, 879
Fnu4HI GCNGC 8 cut(s) 546, 1142, 1560, 2045, 2048, 2051, 2437, 2819
FokI GGATG 4 cut(s) 143, 771, 974, 2589
FriOI GRGCYC 3 cut(s) 2213, 2370, 2592
Fsp4HI GCNGC 8 cut(s) 546, 1142, 1560, 2045, 2048, 2051, 2437, 2819
FspBI CTAG 8 cut(s) 489, 977, 1094, 1221, 1481, 1728, 1842, 2744
GlaI GCGC 2 cut(s) 1943, 2027
GluI GCNGC 8 cut(s) 546, 1142, 1560, 2045, 2048, 2051, 2437, 2819
GsuI CTGGAG 2 cut(s) 1040, 2846
HapII CCGG 5 cut(s) 127, 1667, 1976, 2087, 2693
HgaI GACGC 1 cut(s) 1203
HhaI GCGC 2 cut(s) 1944, 2028
Hin6I GCGC 2 cut(s) 1942, 2026
HinP1I GCGC 2 cut(s) 1942, 2026
HindIII AAGCTT 2 cut(s) 212, 224
HpaII CCGG 5 cut(s) 127, 1667, 1976, 2087, 2693
HphI GGTGA 4 cut(s) 644, 703, 1364, 2092
Hpy166II GTNNAC 2 cut(s) 2189, 2503
Hpy8I GTNNAC 2 cut(s) 2189, 2503
HpyAV CCTTC 7 cut(s) 287, 311, 1253, 1406, 1514, 1935, 2346
HpyCH4III ACNGT 6 cut(s) 15, 659, 720, 1161, 2633, 2878
HpyCH4IV ACGT 4 cut(s) 1018, 1304, 1846, 1952
HpyF10VI GCNNNNNNNGC 7 cut(s) 617, 971, 1150, 1460, 2050, 2151, 2405
HpySE526I ACGT 4 cut(s) 1018, 1304, 1846, 1952
HspAI GCGC 2 cut(s) 1942, 2026
Kpn2I TCCGGA 2 cut(s) 1666, 2086
KpnI GGTACC 1 cut(s) 1925
Ksp22I TGATCA 2 cut(s) 634, 879
LmnI GCTCC 3 cut(s) 1059, 1150, 2332
Lsp1109I GCAGC 7 cut(s) 557, 1153, 2056, 2059, 2062, 2448, 2830
LweI GCATC 5 cut(s) 423, 950, 952, 1546, 2587
MaeI CTAG 8 cut(s) 489, 977, 1094, 1221, 1481, 1728, 1842, 2744
MaeII ACGT 4 cut(s) 1018, 1304, 1846, 1952
MaeIII GTNAC 6 cut(s) 1953, 1985, 2059, 2612, 2762, 2797
MfeI CAATTG 3 cut(s) 638, 1376, 2899
MflI RGATCY 4 cut(s) 481, 1101, 2746, 2869
MhlI GDGCHC 5 cut(s) 79, 1056, 2213, 2370, 2592
MlsI TGGCCA 1 cut(s) 580
MluNI TGGCCA 1 cut(s) 580
MlyI GAGTC 8 cut(s) 389, 1016, 1112, 1661, 1688, 2008, 2711, 2713
MmeI TCCRAC 2 cut(s) 939, 2897
Mox20I TGGCCA 1 cut(s) 580
Mph1103I ATGCAT 2 cut(s) 967, 1477
MroI TCCGGA 2 cut(s) 1666, 2086
MroXI GAANNNNTTC 4 cut(s) 321, 1821, 2530, 2544
MscI TGGCCA 1 cut(s) 580
MseI TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1566, 1880, 2379, 2841
MslI CAYNNNNRTG 5 cut(s) 108, 1187, 2539, 2583, 2712
Msp20I TGGCCA 1 cut(s) 580
MspA1I CMGCKG 4 cut(s) 70, 178, 1253, 2105
MspCI CTTAAG 1 cut(s) 2840
MspI CCGG 5 cut(s) 127, 1667, 1976, 2087, 2693
MspR9I CCNGG 4 cut(s) 1058, 1976, 2733, 2751
MunI CAATTG 3 cut(s) 638, 1376, 2899
MvaI CCWGG 3 cut(s) 1058, 2733, 2751
MwoI GCNNNNNNNGC 7 cut(s) 617, 971, 1150, 1460, 2050, 2151, 2405
NciI CCSGG 1 cut(s) 1976
NlaIV GGNNCC 3 cut(s) 1887, 1923, 2590
NmuCI GTSAC 4 cut(s) 1953, 1985, 2059, 2797
NsiI ATGCAT 2 cut(s) 967, 1477
NspI RCATGY 3 cut(s) 1239, 1475, 2035
PceI AGGCCT 1 cut(s) 1834
PciI ACATGT 1 cut(s) 2031
PcsI WCGNNNNNNNCGW 1 cut(s) 1543
PdmI GAANNNNTTC 4 cut(s) 321, 1821, 2530, 2544
PfeI GAWTC 6 cut(s) 64, 1025, 1090, 1589, 2234, 2270
PflFI GACNNNGTC 1 cut(s) 1651
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 8 cut(s) 547, 1143, 1561, 2046, 2049, 2052, 2438, 2820
PleI GAGTC 8 cut(s) 388, 1015, 1112, 1660, 1687, 2008, 2711, 2712
PpsI GAGTC 8 cut(s) 388, 1015, 1112, 1660, 1687, 2008, 2711, 2712
PpuMI RGGWCCY 1 cut(s) 1885
PscI ACATGT 1 cut(s) 2031
Psp5II RGGWCCY 1 cut(s) 1885
Psp6I CCWGG 3 cut(s) 1056, 2731, 2749
PspEI GGTNACC 1 cut(s) 2762
PspGI CCWGG 3 cut(s) 1056, 2731, 2749
PspN4I GGNNCC 3 cut(s) 1887, 1923, 2590
PspOMI GGGCCC 1 cut(s) 2588
PspPI GGNCC 7 cut(s) 1040, 1885, 2108, 2465, 2588, 2589, 2885
PspPPI RGGWCCY 1 cut(s) 1885
PsuI RGATCY 4 cut(s) 481, 1101, 2746, 2869
PsyI GACNNNGTC 1 cut(s) 1651
PvuII CAGCTG 3 cut(s) 70, 178, 1253
RsaI GTAC 6 cut(s) 125, 1071, 1225, 1923, 2555, 2630
RsaNI GTAC 6 cut(s) 124, 1070, 1224, 1922, 2554, 2629
RseI CAYNNNNRTG 5 cut(s) 108, 1187, 2539, 2583, 2712
SaqAI TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1566, 1880, 2379, 2841
SatI GCNGC 8 cut(s) 546, 1142, 1560, 2045, 2048, 2051, 2437, 2819
Sau96I GGNCC 7 cut(s) 1040, 1885, 2108, 2465, 2588, 2589, 2885
SchI GAGTC 8 cut(s) 389, 1016, 1112, 1661, 1688, 2008, 2711, 2713
ScrFI CCNGG 4 cut(s) 1058, 1976, 2733, 2751
SduI GDGCHC 5 cut(s) 79, 1056, 2213, 2370, 2592
SfaNI GCATC 5 cut(s) 423, 950, 952, 1546, 2587
SfcI CTRYAG 1 cut(s) 391
SinI GGWCC 2 cut(s) 1885, 2885
SmiMI CAYNNNNRTG 5 cut(s) 108, 1187, 2539, 2583, 2712
SmlI CTYRAG 3 cut(s) 2460, 2573, 2840
SmoI CTYRAG 3 cut(s) 2460, 2573, 2840
SseBI AGGCCT 1 cut(s) 1834
SsiI CCGC 2 cut(s) 1560, 2103
SspMI CTAG 8 cut(s) 489, 977, 1094, 1221, 1481, 1728, 1842, 2744
StuI AGGCCT 1 cut(s) 1834
StyD4I CCNGG 4 cut(s) 1056, 1974, 2731, 2749
StyI CCWWGG 4 cut(s) 415, 699, 2617, 2645
TaaI ACNGT 6 cut(s) 15, 659, 720, 1161, 2633, 2878
TaiI ACGT 4 cut(s) 1021, 1307, 1849, 1955
TaqI TCGA 4 cut(s) 1048, 1121, 2702, 2720
TatI WGTACW 3 cut(s) 1069, 1223, 2553
TauI GCSGC 1 cut(s) 1562
TfiI GAWTC 6 cut(s) 64, 1025, 1090, 1589, 2234, 2270
Tru1I TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1566, 1880, 2379, 2841
Tru9I TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1566, 1880, 2379, 2841
TscAI CASTG 3 cut(s) 84, 662, 808
TseFI GTSAC 4 cut(s) 1953, 1985, 2059, 2797
TseI GCWGC 7 cut(s) 545, 1141, 2044, 2047, 2050, 2436, 2818
Tsp45I GTSAC 4 cut(s) 1953, 1985, 2059, 2797
TspGWI ACGGA 1 cut(s) 2301
TspRI CASTG 3 cut(s) 84, 662, 808
Tth111I GACNNNGTC 1 cut(s) 1651
Van91I CCANNNNNTGG 1 cut(s) 587
Vha464I CTTAAG 1 cut(s) 2840
VpaK11BI GGWCC 2 cut(s) 1885, 2885
XapI RAATTY 9 cut(s) 236, 321, 337, 359, 573, 862, 1355, 1628, 2288
XbaI TCTAGA 1 cut(s) 2743
XceI RCATGY 3 cut(s) 1239, 1475, 2035
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XmnI GAANNNNTTC 4 cut(s) 321, 1821, 2530, 2544
XspI CTAG 8 cut(s) 489, 977, 1094, 1221, 1481, 1728, 1842, 2744
Zsp2I ATGCAT 2 cut(s) 967, 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.