Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0002793

Physical Location & Seq

Genomic Coordinates Forward (+)

5593000 .. 5596632

Gene Structure 3633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG066700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCTAAGCCACAGATCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGTTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGACTTGTACCGGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGGATAAAGAAAAGTTCAGCAGCGCCTCAAGATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCATAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAAAGCCGAAATTGGTCAGTTGCAGCCACTCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTGTTGGCGATGTATATTGGTGATGAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTGCAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGTTAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACACTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTTGCATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCCACATTGGAGTCATATCGACGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCATACAGAATCTAGTTAAGATCCCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGATGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCAAGACGTGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCAATGTCACCGAGGCCAAGTGTCCAGAAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATAGACAATTATGAAGGTGGAATGGATTGTCGTTCTCAGCGTATCATTGATGCCGCAATTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCCGGACCGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAGAGTGTCTAGTCAGAAGATTGACAAAGCACCGGAGGAATGTGAGAAACCGATGAGTGATGGCGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGCTTTAAAGGACCCAACCTCTCCAAAACATTCATTGGATCAAAAGGTACCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCCATTTCCGGAAACTCCAATCAGCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCAAACATGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCCATAAGTGACAATGAAGATTCTTTCAGCCATCACGAATTTACAAGCACCTTGTCCTCTCCGTCTTCTTATACTGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATGAGGGGATCAGCACGGCAGATACTGCTTTTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATCAACTGGAAAATCTTGCGGCTTCTAATCCGGCAATGCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGGGAGATCCAACTGTGAGAGGTCCATATATGGACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

EST1 EST1_DNA_bind

Protein Analysis

965

Amino Acids

109.68

Weight (kDa)

5.82

Isoelectric Point (pI)

49.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EST1	PF10374	65 - 170	2.4e-15	Telomerase activating protein Est1
EST1_DNA_bind	PF10373	182 - 490	4.5e-56	Est1 DNA/RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28260 AT1G28260
fragaria_vesca	FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000 FvH4_3g09000
malus_domestica	MD00G1116100.v1.1 MD10G1292700.v1.1
prunus_persica	Prupe.4G049100_v2.0.a1 Prupe.4G049100_v2.0.a1 Prupe.4G049100_v2.0.a1 Prupe.4G049100_v2.0.a1 Prupe.4G049100_v2.0.a1
pyrus_communis	pycom05g28930 pycom10g24480
rosa_chinensis	RchiOBHm_Chr5g0008711
rosa_laevigata	RLG00000031594
rosa_multiflora	Rmu_sc0004761.1_g000007 Rmu_sc0004761.1_g000008
rosa_roxburghii	Rroxscaffold_1G00067880
rosa_rugosa	Rorug04G0437800 Rorug04G0437900 Rorug04G0438000
rosa_samantha	Rh5AG066700 Rh5BG063800 Rh5CG074200 Rh5DG062400
rosa_wichuraiana	Rw5G006220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1921
AccB1I	GGYRCC	1 cut(s)	1921
AccB7I	CCANNNNNTGG	1 cut(s)	587
AccIII	TCCGGA	2 cut(s)	1666, 2086
AciI	CCGC	2 cut(s)	1560, 2681
AcoI	YGGCCR	1 cut(s)	578
AcsI	RAATTY	8 cut(s)	236, 321, 337, 573, 862, 1355, 1628, 2288
AcuI	CTGAAG	3 cut(s)	336, 2214, 2322
AfaI	GTAC	6 cut(s)	125, 1071, 1225, 1923, 2555, 2630
AfeI	AGCGCT	1 cut(s)	2104
AfiI	CCNNNNNNNGG	2 cut(s)	587, 2218
AflII	CTTAAG	1 cut(s)	2834
AflIII	ACRYGT	1 cut(s)	2031
AgsI	TTSAA	7 cut(s)	188, 725, 759, 1205, 1381, 1715, 2662
AjiI	CACGTC	1 cut(s)	1236
AjnI	CCWGG	4 cut(s)	1056, 2725, 2743, 2856
AjuI	GAANNNNNNNTTGG	2 cut(s)	2226, 2258
Alw21I	GWGCWC	1 cut(s)	1056
Alw26I	GTCTC	2 cut(s)	876, 1688
AlwNI	CAGNNNCTG	1 cut(s)	2405
Ama87I	CYCGRG	1 cut(s)	476
Aor13HI	TCCGGA	2 cut(s)	1666, 2086
Aor51HI	AGCGCT	1 cut(s)	2104
ApaI	GGGCCC	1 cut(s)	2592
ApeKI	GCWGC	8 cut(s)	266, 545, 1141, 2044, 2047, 2050, 2436, 2812
ApoI	RAATTY	8 cut(s)	236, 321, 337, 573, 862, 1355, 1628, 2288
ArsI	GACNNNNNNTTYG	2 cut(s)	854, 886
Asp700I	GAANNNNTTC	4 cut(s)	321, 1821, 2530, 2544
Asp718I	GGTACC	1 cut(s)	1921
AspLEI	GCGC	4 cut(s)	271, 1944, 2028, 2105
AspS9I	GGNCC	8 cut(s)	1040, 1669, 1885, 2108, 2465, 2588, 2589, 2879
AsuC2I	CCSGG	1 cut(s)	1976
AsuHPI	GGTGA	4 cut(s)	644, 703, 1364, 1384
AvaI	CYCGRG	1 cut(s)	476
AvaII	GGWCC	3 cut(s)	1669, 1885, 2879
AxyI	CCTNAGG	1 cut(s)	693
BaeGI	GKGCMC	3 cut(s)	79, 1241, 2592
BalI	TGGCCA	1 cut(s)	580
BanI	GGYRCC	1 cut(s)	1921
BanII	GRGCYC	3 cut(s)	2213, 2370, 2592
BbsI	GAAGAC	3 cut(s)	1273, 1437, 2307
Bbv12I	GWGCWC	1 cut(s)	1056
BbvCI	CCTCAGC	1 cut(s)	1442
BbvI	GCAGC	8 cut(s)	278, 557, 1153, 2056, 2059, 2062, 2448, 2824
BccI	CCATC	7 cut(s)	465, 796, 964, 1156, 1772, 2289, 2492
BceAI	ACGGC	2 cut(s)	1929, 2412
BciT130I	CCWGG	4 cut(s)	1058, 2727, 2745, 2858
BciVI	GTATCC	2 cut(s)	850, 2733
BclI	TGATCA	1 cut(s)	879
BcnI	CCSGG	1 cut(s)	1976
BcoDI	GTCTC	2 cut(s)	876, 1688
BfaI	CTAG	8 cut(s)	489, 977, 1094, 1221, 1481, 1728, 1842, 2738
BfmI	CTRYAG	1 cut(s)	391
BfoI	RGCGCY	2 cut(s)	272, 2106
BfrI	CTTAAG	1 cut(s)	2834
BfuI	GTATCC	2 cut(s)	850, 2733
BlpI	GCTNAGC	1 cut(s)	6
Bme1390I	CCNGG	5 cut(s)	1058, 1976, 2727, 2745, 2858
Bme18I	GGWCC	3 cut(s)	1669, 1885, 2879
BmeT110I	CYCGRG	1 cut(s)	476
BmgBI	CACGTC	1 cut(s)	1236
BmgT120I	GGNCC	8 cut(s)	1040, 1669, 1885, 2108, 2465, 2588, 2589, 2879
BmiI	GGNNCC	3 cut(s)	1887, 1923, 2590
BmrFI	CCNGG	5 cut(s)	1058, 1976, 2727, 2745, 2858
BmrI	ACTGGG	1 cut(s)	2604
BmsI	GCATC	5 cut(s)	423, 950, 952, 1546, 2587
BmuI	ACTGGG	1 cut(s)	2604
BpiI	GAAGAC	3 cut(s)	1273, 1437, 2307
BpmI	CTGGAG	2 cut(s)	1040, 2840
Bpu10I	CCTNAGC	1 cut(s)	1442
Bpu1102I	GCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	3 cut(s)	258, 2445, 2594
BpuMI	CCSGG	1 cut(s)	1976
BsaBI	GATNNNNATC	1 cut(s)	1079
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	7 cut(s)	415, 475, 699, 1395, 2617, 2645, 2857
BsaWI	WCCGGW	5 cut(s)	126, 1666, 1671, 1750, 2086
Bsc4I	CCNNNNNNNGG	2 cut(s)	587, 2218
Bse1I	ACTGG	4 cut(s)	1457, 1859, 2610, 2673
Bse21I	CCTNAGG	1 cut(s)	693
Bse3DI	GCAATG	2 cut(s)	835, 2703
Bse8I	GATNNNNATC	1 cut(s)	1079
BseAI	TCCGGA	2 cut(s)	1666, 2086
BseBI	CCWGG	4 cut(s)	1058, 2727, 2745, 2858
BseDI	CCNNGG	7 cut(s)	415, 475, 699, 1395, 2617, 2645, 2857
BseGI	GGATG	4 cut(s)	156, 784, 967, 2602
BseJI	GATNNNNATC	1 cut(s)	1079
BseLI	CCNNNNNNNGG	2 cut(s)	587, 2218
BseMI	GCAATG	2 cut(s)	835, 2703
BseMII	CTCAG	4 cut(s)	684, 1433, 1556, 1994
BseNI	ACTGG	4 cut(s)	1457, 1859, 2610, 2673
BseSI	GKGCMC	3 cut(s)	79, 1241, 2592
BseXI	GCAGC	8 cut(s)	278, 557, 1153, 2056, 2059, 2062, 2448, 2824
BsgI	GTGCAG	1 cut(s)	2502
BshNI	GGYRCC	1 cut(s)	1921
BsiHKAI	GWGCWC	1 cut(s)	1056
BsiHKCI	CYCGRG	1 cut(s)	476
BsiSI	CCGG	7 cut(s)	127, 1667, 1672, 1751, 1976, 2087, 2693
BslFI	GGGAC	3 cut(s)	1206, 2194, 2466
BslI	CCNNNNNNNGG	2 cut(s)	587, 2218
BsmAI	GTCTC	2 cut(s)	876, 1688
BsmFI	GGGAC	3 cut(s)	1206, 2194, 2466
BsmI	GAATGC	1 cut(s)	365
Bso31I	GGTCTC	1 cut(s)	876
BsoBI	CYCGRG	1 cut(s)	476
Bsp120I	GGGCCC	1 cut(s)	2588
Bsp1286I	GDGCHC	6 cut(s)	79, 1056, 1241, 2213, 2370, 2592
Bsp13I	TCCGGA	2 cut(s)	1666, 2086
Bsp1720I	GCTNAGC	1 cut(s)	6
BspACI	CCGC	2 cut(s)	1560, 2681
BspCNI	CTCAG	4 cut(s)	685, 1434, 1555, 1993
BspEI	TCCGGA	2 cut(s)	1666, 2086
BspLI	GGNNCC	3 cut(s)	1887, 1923, 2590
BspT107I	GGYRCC	1 cut(s)	1921
BspTI	CTTAAG	1 cut(s)	2834
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	2 cut(s)	835, 2703
BsrI	ACTGG	4 cut(s)	1457, 1859, 2610, 2673
BssECI	CCNNGG	7 cut(s)	415, 475, 699, 1395, 2617, 2645, 2857
BssT1I	CCWWGG	4 cut(s)	415, 699, 2617, 2645
Bst2UI	CCWGG	4 cut(s)	1058, 2727, 2745, 2858
Bst4CI	ACNGT	4 cut(s)	659, 720, 2633, 2872
Bst6I	CTCTTC	2 cut(s)	372, 1803
BstAFI	CTTAAG	1 cut(s)	2834
BstAPI	GCANNNNNTGC	1 cut(s)	2405
BstC8I	GCNNGC	5 cut(s)	55, 645, 1218, 2107, 2434
BstDEI	CTNAG	9 cut(s)	6, 693, 890, 1442, 1542, 1790, 1980, 2120, 2801
BstEII	GGTNACC	1 cut(s)	2756
BstF5I	GGATG	4 cut(s)	156, 784, 967, 2602
BstH2I	RGCGCY	2 cut(s)	272, 2106
BstHHI	GCGC	4 cut(s)	271, 1944, 2028, 2105
BstMAI	GTCTC	2 cut(s)	876, 1688
BstMWI	GCNNNNNNNGC	7 cut(s)	617, 971, 1150, 1460, 2050, 2151, 2405
BstNI	CCWGG	4 cut(s)	1058, 2727, 2745, 2858
BstNSI	RCATGY	2 cut(s)	1475, 2035
BstPI	GGTNACC	1 cut(s)	2756
BstSCI	CCNGG	5 cut(s)	1056, 1974, 2725, 2743, 2856
BstSFI	CTRYAG	1 cut(s)	391
BstSLI	GKGCMC	3 cut(s)	79, 1241, 2592
BstV1I	GCAGC	8 cut(s)	278, 557, 1153, 2056, 2059, 2062, 2448, 2824
BstV2I	GAAGAC	3 cut(s)	1273, 1437, 2307
BstX2I	RGATCY	4 cut(s)	481, 1101, 2740, 2863
BstYI	RGATCY	4 cut(s)	481, 1101, 2740, 2863
Bsu36I	CCTNAGG	1 cut(s)	693
BsuI	GTATCC	2 cut(s)	850, 2733
BtgZI	GCGATG	2 cut(s)	443, 635
BtrI	CACGTC	1 cut(s)	1236
BtsCI	GGATG	4 cut(s)	156, 784, 967, 2602
BtsI	GCAGTG	1 cut(s)	808
BtsIMutI	CAGTG	3 cut(s)	77, 655, 808
Cac8I	GCNNGC	5 cut(s)	55, 645, 1218, 2107, 2434
CaiI	CAGNNNCTG	1 cut(s)	2405
CfoI	GCGC	4 cut(s)	271, 1944, 2028, 2105
Cfr13I	GGNCC	8 cut(s)	1040, 1669, 1885, 2108, 2465, 2588, 2589, 2879
CpoI	CGGWCCG	1 cut(s)	1669
CseI	GACGC	1 cut(s)	1203
Csp6I	GTAC	6 cut(s)	124, 1070, 1224, 1922, 2554, 2629
CspI	CGGWCCG	1 cut(s)	1669
CviQI	GTAC	6 cut(s)	124, 1070, 1224, 1922, 2554, 2629
DdeI	CTNAG	9 cut(s)	6, 693, 890, 1442, 1542, 1790, 1980, 2120, 2801
DraI	TTTAAA	1 cut(s)	1881
EaeI	YGGCCR	1 cut(s)	578
Eam1104I	CTCTTC	2 cut(s)	372, 1803
EarI	CTCTTC	2 cut(s)	372, 1803
Eco130I	CCWWGG	4 cut(s)	415, 699, 2617, 2645
Eco147I	AGGCCT	1 cut(s)	1834
Eco24I	GRGCYC	3 cut(s)	2213, 2370, 2592
Eco31I	GGTCTC	1 cut(s)	876
Eco32I	GATATC	1 cut(s)	1664
Eco47I	GGWCC	3 cut(s)	1669, 1885, 2879
Eco47III	AGCGCT	1 cut(s)	2104
Eco57I	CTGAAG	3 cut(s)	336, 2214, 2322
Eco81I	CCTNAGG	1 cut(s)	693
Eco88I	CYCGRG	1 cut(s)	476
Eco91I	GGTNACC	1 cut(s)	2756
EcoO109I	RGGNCCY	2 cut(s)	1040, 1885
EcoO65I	GGTNACC	1 cut(s)	2756
EcoRI	GAATTC	1 cut(s)	321
EcoRII	CCWGG	4 cut(s)	1056, 2725, 2743, 2856
EcoRV	GATATC	1 cut(s)	1664
EcoT14I	CCWWGG	4 cut(s)	415, 699, 2617, 2645
EcoT22I	ATGCAT	3 cut(s)	967, 1477, 2703
EcoT38I	GRGCYC	3 cut(s)	2213, 2370, 2592
ErhI	CCWWGG	4 cut(s)	415, 699, 2617, 2645
FalI	AAGNNNNNCTT	2 cut(s)	1809, 1841
FaqI	GGGAC	3 cut(s)	1206, 2194, 2466
FbaI	TGATCA	1 cut(s)	879
FokI	GGATG	4 cut(s)	143, 771, 974, 2589
FriOI	GRGCYC	3 cut(s)	2213, 2370, 2592
FspBI	CTAG	8 cut(s)	489, 977, 1094, 1221, 1481, 1728, 1842, 2738
GlaI	GCGC	4 cut(s)	270, 1943, 2027, 2104
GsuI	CTGGAG	2 cut(s)	1040, 2840
HaeII	RGCGCY	2 cut(s)	272, 2106
HapII	CCGG	7 cut(s)	127, 1667, 1672, 1751, 1976, 2087, 2693
HgaI	GACGC	1 cut(s)	1203
HhaI	GCGC	4 cut(s)	271, 1944, 2028, 2105
Hin6I	GCGC	4 cut(s)	269, 1942, 2026, 2103
HinP1I	GCGC	4 cut(s)	269, 1942, 2026, 2103
HindIII	AAGCTT	2 cut(s)	212, 224
HpaII	CCGG	7 cut(s)	127, 1667, 1672, 1751, 1976, 2087, 2693
HphI	GGTGA	4 cut(s)	644, 703, 1364, 1384
Hpy166II	GTNNAC	2 cut(s)	2189, 2503
Hpy8I	GTNNAC	2 cut(s)	2189, 2503
Hpy99I	CGWCG	1 cut(s)	1020
HpyAV	CCTTC	7 cut(s)	287, 311, 1253, 1406, 1514, 1935, 2346
HpyCH4III	ACNGT	4 cut(s)	659, 720, 2633, 2872
HpyCH4IV	ACGT	5 cut(s)	1018, 1235, 1304, 1846, 1952
HpyF10VI	GCNNNNNNNGC	7 cut(s)	617, 971, 1150, 1460, 2050, 2151, 2405
HpyF3I	CTNAG	9 cut(s)	6, 693, 890, 1442, 1542, 1790, 1980, 2120, 2801
HpySE526I	ACGT	5 cut(s)	1018, 1235, 1304, 1846, 1952
HspAI	GCGC	4 cut(s)	269, 1942, 2026, 2103
Kpn2I	TCCGGA	2 cut(s)	1666, 2086
KpnI	GGTACC	1 cut(s)	1925
Ksp22I	TGATCA	1 cut(s)	879
LmnI	GCTCC	3 cut(s)	1059, 1150, 2332
Lsp1109I	GCAGC	8 cut(s)	278, 557, 1153, 2056, 2059, 2062, 2448, 2824
LweI	GCATC	5 cut(s)	423, 950, 952, 1546, 2587
MaeI	CTAG	8 cut(s)	489, 977, 1094, 1221, 1481, 1728, 1842, 2738
MaeII	ACGT	5 cut(s)	1018, 1235, 1304, 1846, 1952
MaeIII	GTNAC	7 cut(s)	1390, 1953, 2059, 2258, 2612, 2756, 2791
MfeI	CAATTG	2 cut(s)	1376, 2893
MflI	RGATCY	4 cut(s)	481, 1101, 2740, 2863
MhlI	GDGCHC	6 cut(s)	79, 1056, 1241, 2213, 2370, 2592
MlsI	TGGCCA	1 cut(s)	580
MluNI	TGGCCA	1 cut(s)	580
MlyI	GAGTC	7 cut(s)	389, 1016, 1112, 1661, 1688, 2008, 2705
MmeI	TCCRAC	2 cut(s)	939, 2891
Mox20I	TGGCCA	1 cut(s)	580
Mph1103I	ATGCAT	3 cut(s)	967, 1477, 2703
MroI	TCCGGA	2 cut(s)	1666, 2086
MroXI	GAANNNNTTC	4 cut(s)	321, 1821, 2530, 2544
MscI	TGGCCA	1 cut(s)	580
MslI	CAYNNNNRTG	5 cut(s)	108, 1187, 2539, 2583, 2706
Msp20I	TGGCCA	1 cut(s)	580
MspA1I	CMGCKG	2 cut(s)	178, 1253
MspCI	CTTAAG	1 cut(s)	2834
MspI	CCGG	7 cut(s)	127, 1667, 1672, 1751, 1976, 2087, 2693
MspR9I	CCNGG	5 cut(s)	1058, 1976, 2727, 2745, 2858
MunI	CAATTG	2 cut(s)	1376, 2893
Mva1269I	GAATGC	1 cut(s)	365
MvaI	CCWGG	4 cut(s)	1058, 2727, 2745, 2858
MwoI	GCNNNNNNNGC	7 cut(s)	617, 971, 1150, 1460, 2050, 2151, 2405
NciI	CCSGG	1 cut(s)	1976
NlaIV	GGNNCC	3 cut(s)	1887, 1923, 2590
NmuCI	GTSAC	5 cut(s)	1390, 1953, 2059, 2258, 2791
NsiI	ATGCAT	3 cut(s)	967, 1477, 2703
NspI	RCATGY	2 cut(s)	1475, 2035
PceI	AGGCCT	1 cut(s)	1834
PciI	ACATGT	1 cut(s)	2031
PcsI	WCGNNNNNNNCGW	1 cut(s)	1543
PctI	GAATGC	1 cut(s)	365
PdmI	GAANNNNTTC	4 cut(s)	321, 1821, 2530, 2544
PfeI	GAWTC	6 cut(s)	64, 1025, 1090, 1589, 2234, 2270
PflFI	GACNNNGTC	1 cut(s)	1651
PflMI	CCANNNNNTGG	1 cut(s)	587
PleI	GAGTC	7 cut(s)	388, 1015, 1112, 1660, 1687, 2008, 2705
PpsI	GAGTC	7 cut(s)	388, 1015, 1112, 1660, 1687, 2008, 2705
PpuMI	RGGWCCY	1 cut(s)	1885
PscI	ACATGT	1 cut(s)	2031
Psp5II	RGGWCCY	1 cut(s)	1885
Psp6I	CCWGG	4 cut(s)	1056, 2725, 2743, 2856
PspEI	GGTNACC	1 cut(s)	2756
PspGI	CCWGG	4 cut(s)	1056, 2725, 2743, 2856
PspN4I	GGNNCC	3 cut(s)	1887, 1923, 2590
PspOMI	GGGCCC	1 cut(s)	2588
PspPI	GGNCC	8 cut(s)	1040, 1669, 1885, 2108, 2465, 2588, 2589, 2879
PspPPI	RGGWCCY	1 cut(s)	1885
PstNI	CAGNNNCTG	1 cut(s)	2405
PsuI	RGATCY	4 cut(s)	481, 1101, 2740, 2863
PsyI	GACNNNGTC	1 cut(s)	1651
PvuII	CAGCTG	2 cut(s)	178, 1253
RsaI	GTAC	6 cut(s)	125, 1071, 1225, 1923, 2555, 2630
RsaNI	GTAC	6 cut(s)	124, 1070, 1224, 1922, 2554, 2629
RseI	CAYNNNNRTG	5 cut(s)	108, 1187, 2539, 2583, 2706
Rsr2I	CGGWCCG	1 cut(s)	1669
RsrII	CGGWCCG	1 cut(s)	1669
Sau96I	GGNCC	8 cut(s)	1040, 1669, 1885, 2108, 2465, 2588, 2589, 2879
SchI	GAGTC	7 cut(s)	389, 1016, 1112, 1661, 1688, 2008, 2705
ScrFI	CCNGG	5 cut(s)	1058, 1976, 2727, 2745, 2858
SduI	GDGCHC	6 cut(s)	79, 1056, 1241, 2213, 2370, 2592
SfaNI	GCATC	5 cut(s)	423, 950, 952, 1546, 2587
SfcI	CTRYAG	1 cut(s)	391
SinI	GGWCC	3 cut(s)	1669, 1885, 2879
SmiMI	CAYNNNNRTG	5 cut(s)	108, 1187, 2539, 2583, 2706
SmlI	CTYRAG	4 cut(s)	273, 2460, 2573, 2834
SmoI	CTYRAG	4 cut(s)	273, 2460, 2573, 2834
SseBI	AGGCCT	1 cut(s)	1834
SsiI	CCGC	2 cut(s)	1560, 2681
SspMI	CTAG	8 cut(s)	489, 977, 1094, 1221, 1481, 1728, 1842, 2738
StuI	AGGCCT	1 cut(s)	1834
StyD4I	CCNGG	5 cut(s)	1056, 1974, 2725, 2743, 2856
StyI	CCWWGG	4 cut(s)	415, 699, 2617, 2645
TaaI	ACNGT	4 cut(s)	659, 720, 2633, 2872
TaiI	ACGT	5 cut(s)	1021, 1238, 1307, 1849, 1955
TaqI	TCGA	4 cut(s)	1015, 1048, 1121, 2714
TatI	WGTACW	3 cut(s)	1069, 1223, 2553
TauI	GCSGC	2 cut(s)	1562, 2684
TfiI	GAWTC	6 cut(s)	64, 1025, 1090, 1589, 2234, 2270
TscAI	CASTG	3 cut(s)	84, 662, 808
TseFI	GTSAC	5 cut(s)	1390, 1953, 2059, 2258, 2791
TseI	GCWGC	8 cut(s)	266, 545, 1141, 2044, 2047, 2050, 2436, 2812
Tsp45I	GTSAC	5 cut(s)	1390, 1953, 2059, 2258, 2791
TspGWI	ACGGA	1 cut(s)	2301
TspRI	CASTG	3 cut(s)	84, 662, 808
Tth111I	GACNNNGTC	1 cut(s)	1651
Van91I	CCANNNNNTGG	1 cut(s)	587
Vha464I	CTTAAG	1 cut(s)	2834
VpaK11BI	GGWCC	3 cut(s)	1669, 1885, 2879
XapI	RAATTY	8 cut(s)	236, 321, 337, 573, 862, 1355, 1628, 2288
XbaI	TCTAGA	1 cut(s)	2737
XceI	RCATGY	2 cut(s)	1475, 2035
XcmI	CCANNNNNNNNNTGG	1 cut(s)	556
XmnI	GAANNNNTTC	4 cut(s)	321, 1821, 2530, 2544
XspI	CTAG	8 cut(s)	489, 977, 1094, 1221, 1481, 1728, 1842, 2738
Zsp2I	ATGCAT	3 cut(s)	967, 1477, 2703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.