RLG00000031594

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002793
Physical Location & Seq
Forward (+)
6552913 .. 6556492
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031594

Sequence Viewer

Length: 2856 bp
ATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGTCAAAGGTCTTTTGCACTCTGATGTTCAGGACTTGTACCGGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGAAAACAGAAAAGTTCAGCAGCGCCTCAACATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTTTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCATAATTAGCTTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAGAGCCGAAATTGGTCAGTTGCAGCCACCCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTATTGGCGATGTACATTGGTGATGAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACTTTATTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGACTGCCAATTTAATTTCTTACATCCATCTGAAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGTTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACGCTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTCGCATCTACTATGAGAGCATTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTATATTGGAGTCATATCAGCGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCATACAGAATCTAGTTAAGATCCCAGAAACAAAAGATTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGTTGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCAAGACATGCCCCCTTCTACCAGCTGTGCTTGTGTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCAATGTCAGTGAGGCCAAGTGTCCAGAAGGTACTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAAATTTTTCATCTCATTGGGAACATATAGACAATTATGAAAGTGGAATGGATTGTCGTTCTCAGCGTATCATCGATGCCGCAATTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACGGAGTCAAATGGATATCCGGACAGGAAAGAGTCAGGAGGATTGGAGACTAACAACTCCGATGTTGAATTGAAAGTGTCTAGTCAGAAGATTGACAAAGCACCAGAAGAATGTGAGAATCCGATGAGTGATGGGGAAAATCTAAGCTCTACTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTACTTTAAAGGACCCAACCTCTCCAAAACATTCATTGAATCAAAAGGTATCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCCATTTGCGGAAACTCCAATCACCGCTGGCCCTCCCTCCCTTAATGCCTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCACACATGGGACAAGCAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCCATAAGTGACAATGAAGATTCTTTCAGCCGTCATGAATTTGCAAGCACCTTATCCTCTCCGTCTTCTTATACAGCTCCTGTGCCGTCAGCCCCTCCTTTATTACCAGATGATGAGCACCTTTGGTTTAATAAGGGGATCAGCATGGCAGATAATGCTTCTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCCTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCACTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATGAATGGGGAAATCTTGCGGCTTCTAATCCGGCAATGCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACTGACGTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGAGAGATCCAACTGTGAGAGGTCCTTATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

952

Amino Acids

108.09

Weight (kDa)

5.84

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 51 - 156 7.4e-15 Telomerase activating protein Est1
EST1_DNA_bind PF10373 168 - 476 7.9e-56 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 517, 545
AccIII TCCGGA 1 cut(s) 1624
AciI CCGC 4 cut(s) 1518, 2045, 2061, 2639
AclWI GGATC 9 cut(s) 34, 447, 885, 1054, 1940, 2161, 2354, 2693, 2816
AcoI YGGCCR 1 cut(s) 536
AcsI RAATTY 9 cut(s) 194, 279, 295, 531, 820, 1313, 1441, 1586, 2246
AcuI CTGAAG 2 cut(s) 294, 2172
AfaI GTAC 7 cut(s) 83, 584, 1029, 1183, 1375, 2513, 2588
AfiI CCNNNNNNNGG 4 cut(s) 517, 545, 1606, 2176
AflII CTTAAG 1 cut(s) 2792
AflIII ACRYGT 1 cut(s) 1989
AjnI CCWGG 5 cut(s) 1014, 2084, 2457, 2683, 2701
AjuI GAANNNNNNNTTGG 2 cut(s) 2184, 2216
Alw21I GWGCWC 2 cut(s) 1014, 2328
Alw26I GTCTC 2 cut(s) 834, 1646
AlwI GGATC 9 cut(s) 34, 447, 885, 1054, 1940, 2161, 2354, 2693, 2816
AlwNI CAGNNNCTG 1 cut(s) 2288
Ama87I CYCGRG 1 cut(s) 434
Aor13HI TCCGGA 1 cut(s) 1624
ApaI GGGCCC 1 cut(s) 2550
ApeKI GCWGC 8 cut(s) 224, 503, 1099, 2002, 2005, 2008, 2394, 2770
ApoI RAATTY 9 cut(s) 194, 279, 295, 531, 820, 1313, 1441, 1586, 2246
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
Asp700I GAANNNNTTC 4 cut(s) 279, 1779, 2488, 2502
AspLEI GCGC 3 cut(s) 229, 1902, 1986
AspS9I GGNCC 7 cut(s) 998, 1843, 2066, 2423, 2546, 2547, 2837
AsuC2I CCSGG 1 cut(s) 1934
AsuHPI GGTGA 3 cut(s) 602, 1322, 2050
AsuII TTCGAA 1 cut(s) 1079
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 1843, 2837
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 2 cut(s) 37, 2550
BalI TGGCCA 1 cut(s) 538
BanII GRGCYC 2 cut(s) 2171, 2550
BbsI GAAGAC 3 cut(s) 1231, 1395, 2265
Bbv12I GWGCWC 2 cut(s) 1014, 2328
BbvCI CCTCAGC 1 cut(s) 1400
BbvI GCAGC 8 cut(s) 236, 515, 1111, 2014, 2017, 2020, 2406, 2782
BccI CCATC 5 cut(s) 423, 754, 922, 1730, 2450
BceAI ACGGC 3 cut(s) 1887, 2223, 2278
BciT130I CCWGG 5 cut(s) 1016, 2086, 2459, 2685, 2703
BciVI GTATCC 2 cut(s) 808, 2691
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 1 cut(s) 1934
BcoDI GTCTC 2 cut(s) 834, 1646
BfaI CTAG 7 cut(s) 447, 935, 1052, 1179, 1686, 1800, 2696
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 230
BfrI CTTAAG 1 cut(s) 2792
BfuI GTATCC 2 cut(s) 808, 2691
Bme1390I CCNGG 6 cut(s) 1016, 1934, 2086, 2459, 2685, 2703
Bme18I GGWCC 2 cut(s) 1843, 2837
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 7 cut(s) 998, 1843, 2066, 2423, 2546, 2547, 2837
BmiI GGNNCC 2 cut(s) 1845, 2548
BmrFI CCNGG 6 cut(s) 1016, 1934, 2086, 2459, 2685, 2703
BmsI GCATC 5 cut(s) 381, 908, 910, 1504, 2545
BpiI GAAGAC 3 cut(s) 1231, 1395, 2265
BpmI CTGGAG 2 cut(s) 998, 2798
Bpu10I CCTNAGC 1 cut(s) 1400
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 2 cut(s) 2403, 2552
BpuMI CCSGG 1 cut(s) 1934
Bsa29I ATCGAT 1 cut(s) 1512
BsaBI GATNNNNATC 1 cut(s) 1037
BsaI GGTCTC 1 cut(s) 834
BsaJI CCNNGG 5 cut(s) 433, 657, 2085, 2575, 2603
BsaWI WCCGGW 2 cut(s) 84, 1624
Bsc4I CCNNNNNNNGG 4 cut(s) 517, 545, 1606, 2176
Bse1I ACTGG 2 cut(s) 1415, 1817
Bse21I CCTNAGG 1 cut(s) 651
Bse3DI GCAATG 2 cut(s) 793, 2661
Bse8I GATNNNNATC 1 cut(s) 1037
BseAI TCCGGA 1 cut(s) 1624
BseBI CCWGG 5 cut(s) 1016, 2086, 2459, 2685, 2703
BseCI ATCGAT 1 cut(s) 1512
BseDI CCNNGG 5 cut(s) 433, 657, 2085, 2575, 2603
BseGI GGATG 4 cut(s) 114, 742, 925, 2560
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 4 cut(s) 517, 545, 1606, 2176
BseMI GCAATG 2 cut(s) 793, 2661
BseMII CTCAG 4 cut(s) 642, 1391, 1514, 1952
BseNI ACTGG 2 cut(s) 1415, 1817
BseSI GKGCMC 2 cut(s) 37, 2550
BseXI GCAGC 8 cut(s) 236, 515, 1111, 2014, 2017, 2020, 2406, 2782
BsgI GTGCAG 1 cut(s) 2460
BshVI ATCGAT 1 cut(s) 1512
BsiHKAI GWGCWC 2 cut(s) 1014, 2328
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 4 cut(s) 85, 1625, 1934, 2651
BslFI GGGAC 3 cut(s) 1164, 2152, 2424
BslI CCNNNNNNNGG 4 cut(s) 517, 545, 1606, 2176
BsmAI GTCTC 2 cut(s) 834, 1646
BsmFI GGGAC 3 cut(s) 1164, 2152, 2424
BsmI GAATGC 1 cut(s) 323
Bso31I GGTCTC 1 cut(s) 834
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 1079
Bsp120I GGGCCC 1 cut(s) 2546
Bsp1286I GDGCHC 5 cut(s) 37, 1014, 2171, 2328, 2550
Bsp13I TCCGGA 1 cut(s) 1624
Bsp1407I TGTACA 1 cut(s) 582
BspACI CCGC 4 cut(s) 1518, 2045, 2061, 2639
BspCNI CTCAG 4 cut(s) 643, 1392, 1513, 1951
BspDI ATCGAT 1 cut(s) 1512
BspEI TCCGGA 1 cut(s) 1624
BspHI TCATGA 1 cut(s) 2242
BspLI GGNNCC 2 cut(s) 1845, 2548
BspPI GGATC 9 cut(s) 34, 447, 885, 1054, 1940, 2161, 2354, 2693, 2816
BspT104I TTCGAA 1 cut(s) 1079
BspTI CTTAAG 1 cut(s) 2792
BspTNI GGTCTC 1 cut(s) 834
BsrDI GCAATG 2 cut(s) 793, 2661
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 2 cut(s) 1415, 1817
BssECI CCNNGG 5 cut(s) 433, 657, 2085, 2575, 2603
BssT1I CCWWGG 3 cut(s) 657, 2575, 2603
Bst2UI CCWGG 5 cut(s) 1016, 2086, 2459, 2685, 2703
Bst4CI ACNGT 4 cut(s) 617, 1119, 2591, 2830
Bst6I CTCTTC 2 cut(s) 330, 1761
BstAFI CTTAAG 1 cut(s) 2792
BstAPI GCANNNNNTGC 2 cut(s) 920, 2363
BstAUI TGTACA 1 cut(s) 582
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 6 cut(s) 13, 603, 1176, 2065, 2254, 2392
BstDEI CTNAG 6 cut(s) 651, 848, 1400, 1500, 1748, 1938
BstEII GGTNACC 1 cut(s) 2714
BstF5I GGATG 4 cut(s) 114, 742, 925, 2560
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 3 cut(s) 229, 1902, 1986
BstMAI GTCTC 2 cut(s) 834, 1646
BstMWI GCNNNNNNNGC 9 cut(s) 369, 575, 920, 929, 1108, 1418, 2008, 2109, 2363
BstNI CCWGG 5 cut(s) 1016, 2086, 2459, 2685, 2703
BstNSI RCATGY 3 cut(s) 1197, 1433, 1993
BstPI GGTNACC 1 cut(s) 2714
BstSCI CCNGG 6 cut(s) 1014, 1932, 2084, 2457, 2683, 2701
BstSFI CTRYAG 1 cut(s) 349
BstSLI GKGCMC 2 cut(s) 37, 2550
BstV1I GCAGC 8 cut(s) 236, 515, 1111, 2014, 2017, 2020, 2406, 2782
BstV2I GAAGAC 3 cut(s) 1231, 1395, 2265
BstX2I RGATCY 4 cut(s) 439, 1059, 2698, 2821
BstYI RGATCY 4 cut(s) 439, 1059, 2698, 2821
Bsu15I ATCGAT 1 cut(s) 1512
Bsu36I CCTNAGG 1 cut(s) 651
BsuI GTATCC 2 cut(s) 808, 2691
BsuTUI ATCGAT 1 cut(s) 1512
BtgZI GCGATG 2 cut(s) 401, 593
BtsCI GGATG 4 cut(s) 114, 742, 925, 2560
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 4 cut(s) 35, 613, 766, 1357
Cac8I GCNNGC 6 cut(s) 13, 603, 1176, 2065, 2254, 2392
CaiI CAGNNNCTG 1 cut(s) 2288
CciI TCATGA 1 cut(s) 2242
CfoI GCGC 3 cut(s) 229, 1902, 1986
Cfr13I GGNCC 7 cut(s) 998, 1843, 2066, 2423, 2546, 2547, 2837
ClaI ATCGAT 1 cut(s) 1512
CseI GACGC 2 cut(s) 853, 1161
Csp6I GTAC 7 cut(s) 82, 583, 1028, 1182, 1374, 2512, 2587
CspCI CAANNNNNGTGG 2 cut(s) 2557, 2592
CviQI GTAC 7 cut(s) 82, 583, 1028, 1182, 1374, 2512, 2587
DdeI CTNAG 6 cut(s) 651, 848, 1400, 1500, 1748, 1938
DraI TTTAAA 1 cut(s) 1839
EaeI YGGCCR 1 cut(s) 536
Eam1104I CTCTTC 2 cut(s) 330, 1761
EarI CTCTTC 2 cut(s) 330, 1761
Eco130I CCWWGG 3 cut(s) 657, 2575, 2603
Eco147I AGGCCT 1 cut(s) 1792
Eco24I GRGCYC 2 cut(s) 2171, 2550
Eco31I GGTCTC 1 cut(s) 834
Eco32I GATATC 1 cut(s) 1622
Eco47I GGWCC 2 cut(s) 1843, 2837
Eco57I CTGAAG 2 cut(s) 294, 2172
Eco81I CCTNAGG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 2714
EcoO109I RGGNCCY 3 cut(s) 998, 1843, 2837
EcoO65I GGTNACC 1 cut(s) 2714
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 5 cut(s) 1014, 2084, 2457, 2683, 2701
EcoRV GATATC 1 cut(s) 1622
EcoT14I CCWWGG 3 cut(s) 657, 2575, 2603
EcoT22I ATGCAT 3 cut(s) 925, 1435, 2661
EcoT38I GRGCYC 2 cut(s) 2171, 2550
ErhI CCWWGG 3 cut(s) 657, 2575, 2603
FalI AAGNNNNNCTT 2 cut(s) 1767, 1799
FaqI GGGAC 3 cut(s) 1164, 2152, 2424
FbaI TGATCA 1 cut(s) 837
FokI GGATG 4 cut(s) 101, 729, 932, 2547
FriOI GRGCYC 2 cut(s) 2171, 2550
FspBI CTAG 7 cut(s) 447, 935, 1052, 1179, 1686, 1800, 2696
GlaI GCGC 3 cut(s) 228, 1901, 1985
GsuI CTGGAG 2 cut(s) 998, 2798
HaeII RGCGCY 1 cut(s) 230
HapII CCGG 4 cut(s) 85, 1625, 1934, 2651
HgaI GACGC 2 cut(s) 853, 1161
HhaI GCGC 3 cut(s) 229, 1902, 1986
Hin6I GCGC 3 cut(s) 227, 1900, 1984
HinP1I GCGC 3 cut(s) 227, 1900, 1984
HindIII AAGCTT 2 cut(s) 170, 182
HpaII CCGG 4 cut(s) 85, 1625, 1934, 2651
HphI GGTGA 3 cut(s) 602, 1322, 2050
HpyAV CCTTC 4 cut(s) 245, 269, 1211, 1364
HpyCH4III ACNGT 4 cut(s) 617, 1119, 2591, 2830
HpyCH4IV ACGT 4 cut(s) 1262, 1804, 1910, 2757
HpyF10VI GCNNNNNNNGC 9 cut(s) 369, 575, 920, 929, 1108, 1418, 2008, 2109, 2363
HpyF3I CTNAG 6 cut(s) 651, 848, 1400, 1500, 1748, 1938
HpySE526I ACGT 4 cut(s) 1262, 1804, 1910, 2757
HspAI GCGC 3 cut(s) 227, 1900, 1984
Kpn2I TCCGGA 1 cut(s) 1624
Ksp22I TGATCA 1 cut(s) 837
LmnI GCTCC 3 cut(s) 1017, 1108, 2290
Lsp1109I GCAGC 8 cut(s) 236, 515, 1111, 2014, 2017, 2020, 2406, 2782
LweI GCATC 5 cut(s) 381, 908, 910, 1504, 2545
MaeI CTAG 7 cut(s) 447, 935, 1052, 1179, 1686, 1800, 2696
MaeII ACGT 4 cut(s) 1262, 1804, 1910, 2757
MaeIII GTNAC 5 cut(s) 1911, 2017, 2216, 2714, 2749
MfeI CAATTG 2 cut(s) 1334, 2851
MflI RGATCY 4 cut(s) 439, 1059, 2698, 2821
MhlI GDGCHC 5 cut(s) 37, 1014, 2171, 2328, 2550
MlsI TGGCCA 1 cut(s) 538
MluNI TGGCCA 1 cut(s) 538
MlyI GAGTC 6 cut(s) 347, 974, 1619, 1646, 1966, 2663
MmeI TCCRAC 1 cut(s) 2849
Mox20I TGGCCA 1 cut(s) 538
Mph1103I ATGCAT 3 cut(s) 925, 1435, 2661
MroI TCCGGA 1 cut(s) 1624
MroXI GAANNNNTTC 4 cut(s) 279, 1779, 2488, 2502
MscI TGGCCA 1 cut(s) 538
MslI CAYNNNNRTG 6 cut(s) 66, 1145, 2497, 2541, 2619, 2664
Msp20I TGGCCA 1 cut(s) 538
MspA1I CMGCKG 4 cut(s) 28, 136, 1211, 2063
MspCI CTTAAG 1 cut(s) 2792
MspI CCGG 4 cut(s) 85, 1625, 1934, 2651
MspR9I CCNGG 6 cut(s) 1016, 1934, 2086, 2459, 2685, 2703
MunI CAATTG 2 cut(s) 1334, 2851
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 5 cut(s) 1016, 2086, 2459, 2685, 2703
MwoI GCNNNNNNNGC 9 cut(s) 369, 575, 920, 929, 1108, 1418, 2008, 2109, 2363
NciI CCSGG 1 cut(s) 1934
NlaIV GGNNCC 2 cut(s) 1845, 2548
NmuCI GTSAC 4 cut(s) 1911, 2017, 2216, 2749
NsiI ATGCAT 3 cut(s) 925, 1435, 2661
NspI RCATGY 3 cut(s) 1197, 1433, 1993
NspV TTCGAA 1 cut(s) 1079
PagI TCATGA 1 cut(s) 2242
PceI AGGCCT 1 cut(s) 1792
PciI ACATGT 1 cut(s) 1989
PcsI WCGNNNNNNNCGW 1 cut(s) 1501
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 4 cut(s) 279, 1779, 2488, 2502
PfeI GAWTC 9 cut(s) 22, 983, 1048, 1076, 1547, 1723, 1870, 2192, 2228
PflFI GACNNNGTC 1 cut(s) 1609
PflMI CCANNNNNTGG 2 cut(s) 517, 545
PfoI TCCNGGA 1 cut(s) 2457
PleI GAGTC 6 cut(s) 346, 973, 1618, 1645, 1966, 2663
PpsI GAGTC 6 cut(s) 346, 973, 1618, 1645, 1966, 2663
PpuMI RGGWCCY 2 cut(s) 1843, 2837
PscI ACATGT 1 cut(s) 1989
Psp5II RGGWCCY 2 cut(s) 1843, 2837
Psp6I CCWGG 5 cut(s) 1014, 2084, 2457, 2683, 2701
PspEI GGTNACC 1 cut(s) 2714
PspGI CCWGG 5 cut(s) 1014, 2084, 2457, 2683, 2701
PspN4I GGNNCC 2 cut(s) 1845, 2548
PspOMI GGGCCC 1 cut(s) 2546
PspPI GGNCC 7 cut(s) 998, 1843, 2066, 2423, 2546, 2547, 2837
PspPPI RGGWCCY 2 cut(s) 1843, 2837
PstNI CAGNNNCTG 1 cut(s) 2288
PsuI RGATCY 4 cut(s) 439, 1059, 2698, 2821
PsyI GACNNNGTC 1 cut(s) 1609
PvuII CAGCTG 3 cut(s) 28, 136, 1211
RsaI GTAC 7 cut(s) 83, 584, 1029, 1183, 1375, 2513, 2588
RsaNI GTAC 7 cut(s) 82, 583, 1028, 1182, 1374, 2512, 2587
RseI CAYNNNNRTG 6 cut(s) 66, 1145, 2497, 2541, 2619, 2664
Sau96I GGNCC 7 cut(s) 998, 1843, 2066, 2423, 2546, 2547, 2837
SchI GAGTC 6 cut(s) 347, 974, 1619, 1646, 1966, 2663
ScrFI CCNGG 6 cut(s) 1016, 1934, 2086, 2459, 2685, 2703
SduI GDGCHC 5 cut(s) 37, 1014, 2171, 2328, 2550
SfaNI GCATC 5 cut(s) 381, 908, 910, 1504, 2545
SfcI CTRYAG 1 cut(s) 349
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 2 cut(s) 1843, 2837
SmiMI CAYNNNNRTG 6 cut(s) 66, 1145, 2497, 2541, 2619, 2664
SmlI CTYRAG 3 cut(s) 2418, 2531, 2792
SmoI CTYRAG 3 cut(s) 2418, 2531, 2792
SseBI AGGCCT 1 cut(s) 1792
SsiI CCGC 4 cut(s) 1518, 2045, 2061, 2639
SspMI CTAG 7 cut(s) 447, 935, 1052, 1179, 1686, 1800, 2696
StuI AGGCCT 1 cut(s) 1792
StyD4I CCNGG 6 cut(s) 1014, 1932, 2084, 2457, 2683, 2701
StyI CCWWGG 3 cut(s) 657, 2575, 2603
TaaI ACNGT 4 cut(s) 617, 1119, 2591, 2830
TaiI ACGT 4 cut(s) 1265, 1807, 1913, 2760
TaqI TCGA 4 cut(s) 1006, 1079, 1512, 2672
TatI WGTACW 4 cut(s) 582, 1027, 1181, 2511
TauI GCSGC 2 cut(s) 1520, 2642
TfiI GAWTC 9 cut(s) 22, 983, 1048, 1076, 1547, 1723, 1870, 2192, 2228
TscAI CASTG 4 cut(s) 42, 620, 766, 1357
TseFI GTSAC 4 cut(s) 1911, 2017, 2216, 2749
TseI GCWGC 8 cut(s) 224, 503, 1099, 2002, 2005, 2008, 2394, 2770
Tsp45I GTSAC 4 cut(s) 1911, 2017, 2216, 2749
TspGWI ACGGA 2 cut(s) 1622, 2259
TspRI CASTG 4 cut(s) 42, 620, 766, 1357
Tth111I GACNNNGTC 1 cut(s) 1609
Van91I CCANNNNNTGG 2 cut(s) 517, 545
Vha464I CTTAAG 1 cut(s) 2792
VpaK11BI GGWCC 2 cut(s) 1843, 2837
XapI RAATTY 9 cut(s) 194, 279, 295, 531, 820, 1313, 1441, 1586, 2246
XbaI TCTAGA 1 cut(s) 2695
XceI RCATGY 3 cut(s) 1197, 1433, 1993
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmnI GAANNNNTTC 4 cut(s) 279, 1779, 2488, 2502
XspI CTAG 7 cut(s) 447, 935, 1052, 1179, 1686, 1800, 2696
Zsp2I ATGCAT 3 cut(s) 925, 1435, 2661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.