Rroxscaffold_1G00067880

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002793
Physical Location & Seq
Reverse (-)
89164147 .. 89169878
5732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067880.1

Sequence Viewer

Length: 2901 bp
ATGACGGCTAAGCCACAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGGCTTGTACCGGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATCCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGAATAAAGAAAAGTTCAGCAGCGCCTCAACATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCAAAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTTGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAAAGCCGAAATTGGTCAGTTGCAGCCACCCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTATTGGCGATGTACATTGGTGATGAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTGAAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACGCTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTCACATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTACATTGGAGTCATATCAGCGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCATACAGAATCTAGTTAAGATCCCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGAACATGGAGCTGACACAATTGGGATTGACTGCCACATTCATAATCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCGAGACATGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGCCAATGTCGGTGAGGCCAAGTGTCCAGAAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATAGACAATTATGAAAGTGGAATGGATTTTCGTTCTCAGCGTATCATCGATGCCGCATTTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTTTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCTGGACAGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAAAGTGTCTAGTCAGAAGATTGACAAAGCACCAGAAGAATGTGAGAAACCGATGAGTGATGGGGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTCACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGGTTTAAAGAACCCAACCTCTCCAAAACATTCATTGGATCAAAAGGTGCCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGGAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCTATTTCCGGAAATTTCAATCACCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCACACTTGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCCATAAGTGACAATGAAGATTCTTTCAGCCATCCCGAATTTGCAAGCACCTTATCCTCTCCGTCTTCTTATACAGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATAAGGAGATCAGCATGGCAGATAATGCTTCTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCCTCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGATTCATCATTTCAATCAATGGGGAAATCTTGCGGCTTCTAATCCGGCAATGCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTCTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAAAGAGAGAAGCAACTCCAGAGAGATCCAACTGTGAGAGGTCCTTATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

966

Amino Acids

109.82

Weight (kDa)

5.82

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 66 - 171 7.2e-16 Telomerase activating protein Est1
EST1_DNA_bind PF10373 183 - 491 1e-55 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1924
AccB7I CCANNNNNTGG 2 cut(s) 562, 590
AccIII TCCGGA 1 cut(s) 2089
AciI CCGC 4 cut(s) 1563, 2029, 2106, 2684
AclWI GGATC 9 cut(s) 79, 492, 930, 1099, 1923, 1985, 2206, 2738, 2861
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 9 cut(s) 239, 324, 340, 576, 865, 1358, 1631, 2094, 2291
AcuI CTGAAG 3 cut(s) 339, 2217, 2325
AfaI GTAC 6 cut(s) 128, 629, 1074, 1228, 2558, 2633
AfiI CCNNNNNNNGG 4 cut(s) 562, 590, 2221, 2544
AflII CTTAAG 1 cut(s) 2837
AflIII ACRYGT 1 cut(s) 2034
AjnI CCWGG 3 cut(s) 1059, 2728, 2746
AjuI GAANNNNNNNTTGG 2 cut(s) 2229, 2261
Alw21I GWGCWC 1 cut(s) 1059
Alw26I GTCTC 4 cut(s) 879, 1229, 1691, 2756
AlwI GGATC 9 cut(s) 79, 492, 930, 1099, 1923, 1985, 2206, 2738, 2861
AlwNI CAGNNNCTG 1 cut(s) 2333
Aor13HI TCCGGA 1 cut(s) 2089
ApaI GGGCCC 1 cut(s) 2595
ApeKI GCWGC 7 cut(s) 269, 548, 2047, 2050, 2053, 2439, 2815
ApoI RAATTY 9 cut(s) 239, 324, 340, 576, 865, 1358, 1631, 2094, 2291
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
Asp700I GAANNNNTTC 4 cut(s) 324, 1824, 2533, 2547
AspLEI GCGC 2 cut(s) 274, 1947
AspS9I GGNCC 6 cut(s) 1043, 2111, 2468, 2591, 2592, 2882
AsuC2I CCSGG 1 cut(s) 1979
AsuHPI GGTGA 5 cut(s) 647, 706, 1367, 1409, 2095
AvaII GGWCC 1 cut(s) 2882
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 2 cut(s) 82, 2595
BalI TGGCCA 1 cut(s) 583
BanI GGYRCC 1 cut(s) 1924
BanII GRGCYC 3 cut(s) 2216, 2373, 2595
BbsI GAAGAC 3 cut(s) 1276, 1440, 2310
Bbv12I GWGCWC 1 cut(s) 1059
BbvCI CCTCAGC 1 cut(s) 1445
BbvI GCAGC 7 cut(s) 281, 560, 2059, 2062, 2065, 2451, 2827
BccI CCATC 6 cut(s) 468, 799, 967, 1775, 2292, 2495
BceAI ACGGC 2 cut(s) 21, 1932
BciT130I CCWGG 3 cut(s) 1061, 2730, 2748
BciVI GTATCC 2 cut(s) 853, 2736
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 1 cut(s) 1979
BcoDI GTCTC 4 cut(s) 879, 1229, 1691, 2756
BfaI CTAG 8 cut(s) 492, 980, 1097, 1224, 1484, 1731, 1845, 2741
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 1 cut(s) 275
BfrI CTTAAG 1 cut(s) 2837
BfuI GTATCC 2 cut(s) 853, 2736
BisI GCNGC 9 cut(s) 270, 549, 1563, 2048, 2051, 2054, 2440, 2685, 2816
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 9 cut(s) 271, 550, 1564, 2049, 2052, 2055, 2441, 2686, 2817
Bme1390I CCNGG 4 cut(s) 1061, 1979, 2730, 2748
Bme18I GGWCC 1 cut(s) 2882
BmgT120I GGNCC 6 cut(s) 1043, 2111, 2468, 2591, 2592, 2882
BmiI GGNNCC 2 cut(s) 1926, 2593
BmrFI CCNGG 4 cut(s) 1061, 1979, 2730, 2748
BmrI ACTGGG 1 cut(s) 2607
BmsI GCATC 4 cut(s) 426, 955, 1549, 2590
BmuI ACTGGG 1 cut(s) 2607
BpiI GAAGAC 3 cut(s) 1276, 1440, 2310
BpmI CTGGAG 2 cut(s) 1043, 2843
Bpu10I CCTNAGC 1 cut(s) 1445
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 2 cut(s) 2448, 2597
BpuMI CCSGG 1 cut(s) 1979
Bsa29I ATCGAT 1 cut(s) 1557
BsaBI GATNNNNATC 1 cut(s) 1082
BsaI GGTCTC 2 cut(s) 879, 2756
BsaJI CCNNGG 5 cut(s) 418, 478, 702, 2620, 2648
BsaWI WCCGGW 2 cut(s) 129, 2089
Bsc4I CCNNNNNNNGG 4 cut(s) 562, 590, 2221, 2544
Bse1I ACTGG 3 cut(s) 1460, 1862, 2613
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 2 cut(s) 838, 2706
Bse8I GATNNNNATC 1 cut(s) 1082
BseAI TCCGGA 1 cut(s) 2089
BseBI CCWGG 3 cut(s) 1061, 2730, 2748
BseCI ATCGAT 1 cut(s) 1557
BseDI CCNNGG 5 cut(s) 418, 478, 702, 2620, 2648
BseGI GGATG 5 cut(s) 159, 787, 970, 2284, 2605
BseJI GATNNNNATC 1 cut(s) 1082
BseLI CCNNNNNNNGG 4 cut(s) 562, 590, 2221, 2544
BseMI GCAATG 2 cut(s) 838, 2706
BseMII CTCAG 4 cut(s) 687, 1436, 1559, 1997
BseNI ACTGG 3 cut(s) 1460, 1862, 2613
BseSI GKGCMC 2 cut(s) 82, 2595
BseXI GCAGC 7 cut(s) 281, 560, 2059, 2062, 2065, 2451, 2827
BsgI GTGCAG 1 cut(s) 2505
BshNI GGYRCC 1 cut(s) 1924
BshVI ATCGAT 1 cut(s) 1557
BsiHKAI GWGCWC 1 cut(s) 1059
BsiSI CCGG 4 cut(s) 130, 1979, 2090, 2696
BslFI GGGAC 3 cut(s) 1209, 2197, 2469
BslI CCNNNNNNNGG 4 cut(s) 562, 590, 2221, 2544
BsmAI GTCTC 4 cut(s) 879, 1229, 1691, 2756
BsmFI GGGAC 3 cut(s) 1209, 2197, 2469
BsmI GAATGC 2 cut(s) 368, 1390
Bso31I GGTCTC 2 cut(s) 879, 2756
Bsp120I GGGCCC 1 cut(s) 2591
Bsp1286I GDGCHC 5 cut(s) 82, 1059, 2216, 2373, 2595
Bsp13I TCCGGA 1 cut(s) 2089
Bsp1407I TGTACA 1 cut(s) 627
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 4 cut(s) 1563, 2029, 2106, 2684
BspCNI CTCAG 4 cut(s) 688, 1437, 1558, 1996
BspDI ATCGAT 1 cut(s) 1557
BspEI TCCGGA 1 cut(s) 2089
BspLI GGNNCC 2 cut(s) 1926, 2593
BspPI GGATC 9 cut(s) 79, 492, 930, 1099, 1923, 1985, 2206, 2738, 2861
BspT107I GGYRCC 1 cut(s) 1924
BspTI CTTAAG 1 cut(s) 2837
BspTNI GGTCTC 2 cut(s) 879, 2756
BsrDI GCAATG 2 cut(s) 838, 2706
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 3 cut(s) 1460, 1862, 2613
BssECI CCNNGG 5 cut(s) 418, 478, 702, 2620, 2648
BssT1I CCWWGG 5 cut(s) 418, 478, 702, 2620, 2648
Bst2UI CCWGG 3 cut(s) 1061, 2730, 2748
Bst4CI ACNGT 5 cut(s) 18, 662, 723, 2636, 2875
Bst6I CTCTTC 2 cut(s) 375, 1806
BstAFI CTTAAG 1 cut(s) 2837
BstAPI GCANNNNNTGC 1 cut(s) 2408
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 6 cut(s) 58, 648, 1221, 2110, 2299, 2437
BstDEI CTNAG 9 cut(s) 9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
BstEII GGTNACC 1 cut(s) 2759
BstF5I GGATG 5 cut(s) 159, 787, 970, 2284, 2605
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 2 cut(s) 274, 1947
BstMAI GTCTC 4 cut(s) 879, 1229, 1691, 2756
BstMWI GCNNNNNNNGC 6 cut(s) 620, 974, 1463, 2053, 2154, 2408
BstNI CCWGG 3 cut(s) 1061, 2730, 2748
BstNSI RCATGY 3 cut(s) 1242, 1478, 2038
BstPI GGTNACC 1 cut(s) 2759
BstSCI CCNGG 4 cut(s) 1059, 1977, 2728, 2746
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 82, 2595
BstV1I GCAGC 7 cut(s) 281, 560, 2059, 2062, 2065, 2451, 2827
BstV2I GAAGAC 3 cut(s) 1276, 1440, 2310
BstX2I RGATCY 4 cut(s) 484, 1104, 2743, 2866
BstYI RGATCY 4 cut(s) 484, 1104, 2743, 2866
Bsu15I ATCGAT 1 cut(s) 1557
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 2 cut(s) 853, 2736
BsuTUI ATCGAT 1 cut(s) 1557
BtgZI GCGATG 2 cut(s) 446, 638
BtsCI GGATG 5 cut(s) 159, 787, 970, 2284, 2605
BtsI GCAGTG 1 cut(s) 811
BtsIMutI CAGTG 3 cut(s) 80, 658, 811
Cac8I GCNNGC 6 cut(s) 58, 648, 1221, 2110, 2299, 2437
CaiI CAGNNNCTG 1 cut(s) 2333
CfoI GCGC 2 cut(s) 274, 1947
Cfr13I GGNCC 6 cut(s) 1043, 2111, 2468, 2591, 2592, 2882
ClaI ATCGAT 1 cut(s) 1557
CseI GACGC 2 cut(s) 898, 1206
Csp6I GTAC 6 cut(s) 127, 628, 1073, 1227, 2557, 2632
CviQI GTAC 6 cut(s) 127, 628, 1073, 1227, 2557, 2632
DdeI CTNAG 9 cut(s) 9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
DraI TTTAAA 2 cut(s) 1570, 1884
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 2 cut(s) 375, 1806
EarI CTCTTC 2 cut(s) 375, 1806
EciI GGCGGA 1 cut(s) 2044
Eco130I CCWWGG 5 cut(s) 418, 478, 702, 2620, 2648
Eco147I AGGCCT 1 cut(s) 1837
Eco24I GRGCYC 3 cut(s) 2216, 2373, 2595
Eco31I GGTCTC 2 cut(s) 879, 2756
Eco32I GATATC 1 cut(s) 1667
Eco47I GGWCC 1 cut(s) 2882
Eco57I CTGAAG 3 cut(s) 339, 2217, 2325
Eco81I CCTNAGG 1 cut(s) 696
Eco91I GGTNACC 1 cut(s) 2759
EcoO109I RGGNCCY 2 cut(s) 1043, 2882
EcoO65I GGTNACC 1 cut(s) 2759
EcoRI GAATTC 1 cut(s) 324
EcoRII CCWGG 3 cut(s) 1059, 2728, 2746
EcoRV GATATC 1 cut(s) 1667
EcoT14I CCWWGG 5 cut(s) 418, 478, 702, 2620, 2648
EcoT22I ATGCAT 3 cut(s) 970, 1480, 2706
EcoT38I GRGCYC 3 cut(s) 2216, 2373, 2595
ErhI CCWWGG 5 cut(s) 418, 478, 702, 2620, 2648
FalI AAGNNNNNCTT 2 cut(s) 1812, 1844
FaqI GGGAC 3 cut(s) 1209, 2197, 2469
FbaI TGATCA 1 cut(s) 882
Fnu4HI GCNGC 9 cut(s) 270, 549, 1563, 2048, 2051, 2054, 2440, 2685, 2816
FokI GGATG 5 cut(s) 146, 774, 977, 2271, 2592
FriOI GRGCYC 3 cut(s) 2216, 2373, 2595
Fsp4HI GCNGC 9 cut(s) 270, 549, 1563, 2048, 2051, 2054, 2440, 2685, 2816
FspBI CTAG 8 cut(s) 492, 980, 1097, 1224, 1484, 1731, 1845, 2741
GlaI GCGC 2 cut(s) 273, 1946
GluI GCNGC 9 cut(s) 270, 549, 1563, 2048, 2051, 2054, 2440, 2685, 2816
GsuI CTGGAG 2 cut(s) 1043, 2843
HaeII RGCGCY 1 cut(s) 275
HapII CCGG 4 cut(s) 130, 1979, 2090, 2696
HgaI GACGC 2 cut(s) 898, 1206
HhaI GCGC 2 cut(s) 274, 1947
Hin6I GCGC 2 cut(s) 272, 1945
HinP1I GCGC 2 cut(s) 272, 1945
HindIII AAGCTT 2 cut(s) 215, 227
HpaII CCGG 4 cut(s) 130, 1979, 2090, 2696
HphI GGTGA 5 cut(s) 647, 706, 1367, 1409, 2095
Hpy166II GTNNAC 2 cut(s) 2192, 2506
Hpy8I GTNNAC 2 cut(s) 2192, 2506
HpyAV CCTTC 6 cut(s) 290, 314, 1256, 1409, 1938, 2349
HpyCH4III ACNGT 5 cut(s) 18, 662, 723, 2636, 2875
HpyCH4IV ACGT 3 cut(s) 1307, 1849, 1955
HpyF10VI GCNNNNNNNGC 6 cut(s) 620, 974, 1463, 2053, 2154, 2408
HpyF3I CTNAG 9 cut(s) 9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
HpySE526I ACGT 3 cut(s) 1307, 1849, 1955
HspAI GCGC 2 cut(s) 272, 1945
Kpn2I TCCGGA 1 cut(s) 2089
Ksp22I TGATCA 1 cut(s) 882
LmnI GCTCC 3 cut(s) 1062, 1153, 2335
Lsp1109I GCAGC 7 cut(s) 281, 560, 2059, 2062, 2065, 2451, 2827
LweI GCATC 4 cut(s) 426, 955, 1549, 2590
MaeI CTAG 8 cut(s) 492, 980, 1097, 1224, 1484, 1731, 1845, 2741
MaeII ACGT 3 cut(s) 1307, 1849, 1955
MaeIII GTNAC 6 cut(s) 1956, 2062, 2261, 2615, 2759, 2794
MfeI CAATTG 3 cut(s) 1163, 1379, 2896
MflI RGATCY 4 cut(s) 484, 1104, 2743, 2866
MhlI GDGCHC 5 cut(s) 82, 1059, 2216, 2373, 2595
MlsI TGGCCA 1 cut(s) 583
MluNI TGGCCA 1 cut(s) 583
MlyI GAGTC 7 cut(s) 392, 1019, 1115, 1664, 1691, 2011, 2708
MmeI TCCRAC 2 cut(s) 942, 2894
Mox20I TGGCCA 1 cut(s) 583
Mph1103I ATGCAT 3 cut(s) 970, 1480, 2706
MroI TCCGGA 1 cut(s) 2089
MroXI GAANNNNTTC 4 cut(s) 324, 1824, 2533, 2547
MscI TGGCCA 1 cut(s) 583
MseI TTAA 9 cut(s) 302, 681, 777, 908, 1101, 1569, 1883, 2382, 2838
MslI CAYNNNNRTG 4 cut(s) 111, 1190, 2586, 2709
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 4 cut(s) 73, 181, 1256, 2108
MspCI CTTAAG 1 cut(s) 2837
MspI CCGG 4 cut(s) 130, 1979, 2090, 2696
MspR9I CCNGG 4 cut(s) 1061, 1979, 2730, 2748
MunI CAATTG 3 cut(s) 1163, 1379, 2896
Mva1269I GAATGC 2 cut(s) 368, 1390
MvaI CCWGG 3 cut(s) 1061, 2730, 2748
MwoI GCNNNNNNNGC 6 cut(s) 620, 974, 1463, 2053, 2154, 2408
NciI CCSGG 1 cut(s) 1979
NlaIV GGNNCC 2 cut(s) 1926, 2593
NmuCI GTSAC 4 cut(s) 1956, 2062, 2261, 2794
NsiI ATGCAT 3 cut(s) 970, 1480, 2706
NspI RCATGY 3 cut(s) 1242, 1478, 2038
PceI AGGCCT 1 cut(s) 1837
PciI ACATGT 1 cut(s) 2034
PcsI WCGNNNNNNNCGW 1 cut(s) 1546
PctI GAATGC 2 cut(s) 368, 1390
PdmI GAANNNNTTC 4 cut(s) 324, 1824, 2533, 2547
PfeI GAWTC 7 cut(s) 67, 1028, 1093, 1592, 2237, 2273, 2653
PflFI GACNNNGTC 1 cut(s) 1654
PflMI CCANNNNNTGG 2 cut(s) 562, 590
PkrI GCNGC 9 cut(s) 271, 550, 1564, 2049, 2052, 2055, 2441, 2686, 2817
PleI GAGTC 7 cut(s) 391, 1018, 1115, 1663, 1690, 2011, 2708
PpsI GAGTC 7 cut(s) 391, 1018, 1115, 1663, 1690, 2011, 2708
PpuMI RGGWCCY 1 cut(s) 2882
PscI ACATGT 1 cut(s) 2034
Psp5II RGGWCCY 1 cut(s) 2882
Psp6I CCWGG 3 cut(s) 1059, 2728, 2746
PspEI GGTNACC 1 cut(s) 2759
PspGI CCWGG 3 cut(s) 1059, 2728, 2746
PspN4I GGNNCC 2 cut(s) 1926, 2593
PspOMI GGGCCC 1 cut(s) 2591
PspPI GGNCC 6 cut(s) 1043, 2111, 2468, 2591, 2592, 2882
PspPPI RGGWCCY 1 cut(s) 2882
PstNI CAGNNNCTG 1 cut(s) 2333
PsuI RGATCY 4 cut(s) 484, 1104, 2743, 2866
PsyI GACNNNGTC 1 cut(s) 1654
PvuII CAGCTG 3 cut(s) 73, 181, 1256
RsaI GTAC 6 cut(s) 128, 629, 1074, 1228, 2558, 2633
RsaNI GTAC 6 cut(s) 127, 628, 1073, 1227, 2557, 2632
RseI CAYNNNNRTG 4 cut(s) 111, 1190, 2586, 2709
SaqAI TTAA 9 cut(s) 302, 681, 777, 908, 1101, 1569, 1883, 2382, 2838
SatI GCNGC 9 cut(s) 270, 549, 1563, 2048, 2051, 2054, 2440, 2685, 2816
Sau96I GGNCC 6 cut(s) 1043, 2111, 2468, 2591, 2592, 2882
SchI GAGTC 7 cut(s) 392, 1019, 1115, 1664, 1691, 2011, 2708
ScrFI CCNGG 4 cut(s) 1061, 1979, 2730, 2748
SduI GDGCHC 5 cut(s) 82, 1059, 2216, 2373, 2595
SfaNI GCATC 4 cut(s) 426, 955, 1549, 2590
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 2882
SmiMI CAYNNNNRTG 4 cut(s) 111, 1190, 2586, 2709
SmlI CTYRAG 3 cut(s) 2463, 2576, 2837
SmoI CTYRAG 3 cut(s) 2463, 2576, 2837
SseBI AGGCCT 1 cut(s) 1837
SsiI CCGC 4 cut(s) 1563, 2029, 2106, 2684
SspMI CTAG 8 cut(s) 492, 980, 1097, 1224, 1484, 1731, 1845, 2741
StuI AGGCCT 1 cut(s) 1837
StyD4I CCNGG 4 cut(s) 1059, 1977, 2728, 2746
StyI CCWWGG 5 cut(s) 418, 478, 702, 2620, 2648
TaaI ACNGT 5 cut(s) 18, 662, 723, 2636, 2875
TaiI ACGT 3 cut(s) 1310, 1852, 1958
TaqI TCGA 5 cut(s) 1051, 1124, 1233, 1557, 2717
TatI WGTACW 4 cut(s) 627, 1072, 1226, 2556
TauI GCSGC 2 cut(s) 1565, 2687
TfiI GAWTC 7 cut(s) 67, 1028, 1093, 1592, 2237, 2273, 2653
Tru1I TTAA 9 cut(s) 302, 681, 777, 908, 1101, 1569, 1883, 2382, 2838
Tru9I TTAA 9 cut(s) 302, 681, 777, 908, 1101, 1569, 1883, 2382, 2838
TscAI CASTG 3 cut(s) 87, 665, 811
TseFI GTSAC 4 cut(s) 1956, 2062, 2261, 2794
TseI GCWGC 7 cut(s) 269, 548, 2047, 2050, 2053, 2439, 2815
Tsp45I GTSAC 4 cut(s) 1956, 2062, 2261, 2794
TspGWI ACGGA 1 cut(s) 2304
TspRI CASTG 3 cut(s) 87, 665, 811
Tth111I GACNNNGTC 1 cut(s) 1654
Van91I CCANNNNNTGG 2 cut(s) 562, 590
Vha464I CTTAAG 1 cut(s) 2837
VpaK11BI GGWCC 1 cut(s) 2882
XapI RAATTY 9 cut(s) 239, 324, 340, 576, 865, 1358, 1631, 2094, 2291
XbaI TCTAGA 1 cut(s) 2740
XceI RCATGY 3 cut(s) 1242, 1478, 2038
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XmnI GAANNNNTTC 4 cut(s) 324, 1824, 2533, 2547
XspI CTAG 8 cut(s) 492, 980, 1097, 1224, 1484, 1731, 1845, 2741
Zsp2I ATGCAT 3 cut(s) 970, 1480, 2706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.