Rh5CG074200

Telomerase activating protein Est1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002793
Physical Location & Seq
Forward (+)
5555905 .. 5567392
11488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG074200.1

Sequence Viewer

Length: 2892 bp
ATGGCTAAGCCACAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGATTTGTACCAGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGGATAAAGAAAAGTTCAGCAGCGCCTCAAGATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCAAAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCTGATGTCCAAAGCCGAAATTGGTCAGTTGCAGCCACTCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTGTTGGCGATGTATATTGGTGATGAATTGCTCGCTTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTGCAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACACTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTTGCATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTACATTGGAGTCATATCGACGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCGTACAGAATCTAGTTAAGATCCCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGTTGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCTAGTACAATCAAGACATGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCAGTGTCAGCAAGTGTCCAGAAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTTTCTCAATCACATGCATTACTAGATTTTTCATCTCACTGGGAACATATAGACAATTATGAAGGTGGAATGGATTGTCGTTCTCAGCGTATCATTGATGCCGCAATTAAAATTGCAAACAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGAAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCCGGACAGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAGAGTGTCTAGTCAGAAGATTGACAAAGCACCAGAGGAATGTGAGAAACCGATGAGTGATGGGGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGCTTTAAAGGACCCAACCTCTCCAAAACATTCAATGGATCAAAAGGTACCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGACTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTGTCACAGATACAATGGCACAGCCATTTCCCGAAACTCCAATCAGCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCACACATGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAATAGCTTCAGAATCTTTGATTGGCCTATCCATAAGTGACAATGAAGATTCTTTCAGCCATCACGAATTTGCAAGCACCTTATCCTCTCCGTCTTCTTATACTGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATGAGGGGATCAGCACGGCAGATAATGCTTTTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATCAACGGGGAAATCTTGCGCCTTCTAATCCGGCAATGCATATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGAGAGATCCAACTGTGAGAGGTCCATATATGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000723 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

963

Amino Acids

109.57

Weight (kDa)

5.9

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 65 - 170 9.1e-16 Telomerase activating protein Est1
EST1_DNA_bind PF10373 182 - 488 6.8e-56 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1915
AccB1I GGYRCC 1 cut(s) 1915
AccB7I CCANNNNNTGG 1 cut(s) 587
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 1 cut(s) 1554
AcoI YGGCCR 1 cut(s) 578
AcsI RAATTY 8 cut(s) 236, 321, 337, 573, 862, 1355, 1622, 2282
AcuI CTGAAG 3 cut(s) 336, 2208, 2316
AfaI GTAC 7 cut(s) 125, 1071, 1086, 1225, 1917, 2549, 2624
AfeI AGCGCT 1 cut(s) 2098
AfiI CCNNNNNNNGG 2 cut(s) 587, 2212
AflII CTTAAG 1 cut(s) 2828
AflIII ACRYGT 1 cut(s) 2025
AgsI TTSAA 9 cut(s) 19, 188, 725, 759, 1205, 1381, 1709, 1902, 2656
AjnI CCWGG 3 cut(s) 1056, 2719, 2737
AjuI GAANNNNNNNTTGG 2 cut(s) 2220, 2252
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 2 cut(s) 876, 1682
Ama87I CYCGRG 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 1660
Aor51HI AGCGCT 1 cut(s) 2098
ApaI GGGCCC 1 cut(s) 2586
ApeKI GCWGC 8 cut(s) 266, 545, 1141, 2038, 2041, 2044, 2430, 2806
ApoI RAATTY 8 cut(s) 236, 321, 337, 573, 862, 1355, 1622, 2282
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
Asp700I GAANNNNTTC 4 cut(s) 321, 1815, 2524, 2538
Asp718I GGTACC 1 cut(s) 1915
AspLEI GCGC 5 cut(s) 271, 1938, 2022, 2099, 2677
AspS9I GGNCC 7 cut(s) 1040, 1879, 2102, 2459, 2582, 2583, 2873
AsuHPI GGTGA 3 cut(s) 644, 703, 1364
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 2 cut(s) 1879, 2873
AxyI CCTNAGG 1 cut(s) 693
BaeGI GKGCMC 2 cut(s) 79, 2586
BalI TGGCCA 1 cut(s) 580
BanI GGYRCC 1 cut(s) 1915
BanII GRGCYC 3 cut(s) 2207, 2364, 2586
BbsI GAAGAC 3 cut(s) 1273, 1431, 2301
Bbv12I GWGCWC 1 cut(s) 1056
BbvCI CCTCAGC 1 cut(s) 1436
BbvI GCAGC 8 cut(s) 278, 557, 1153, 2050, 2053, 2056, 2442, 2818
BccI CCATC 6 cut(s) 465, 796, 964, 1766, 2283, 2486
BceAI ACGGC 2 cut(s) 1923, 2406
BciT130I CCWGG 3 cut(s) 1058, 2721, 2739
BciVI GTATCC 2 cut(s) 850, 2727
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 2 cut(s) 876, 1682
BfaI CTAG 8 cut(s) 489, 977, 1094, 1221, 1475, 1722, 1836, 2732
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 2 cut(s) 272, 2100
BfrI CTTAAG 1 cut(s) 2828
BfuI GTATCC 2 cut(s) 850, 2727
BisI GCNGC 9 cut(s) 267, 546, 1142, 1554, 2039, 2042, 2045, 2431, 2807
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 9 cut(s) 268, 547, 1143, 1555, 2040, 2043, 2046, 2432, 2808
Bme1390I CCNGG 3 cut(s) 1058, 2721, 2739
Bme18I GGWCC 2 cut(s) 1879, 2873
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 7 cut(s) 1040, 1879, 2102, 2459, 2582, 2583, 2873
BmiI GGNNCC 3 cut(s) 1881, 1917, 2584
BmrFI CCNGG 3 cut(s) 1058, 2721, 2739
BmrI ACTGGG 2 cut(s) 1501, 2598
BmsI GCATC 5 cut(s) 423, 950, 952, 1540, 2581
BmuI ACTGGG 2 cut(s) 1501, 2598
BpiI GAAGAC 3 cut(s) 1273, 1431, 2301
BpmI CTGGAG 2 cut(s) 1040, 2834
Bpu10I CCTNAGC 1 cut(s) 1436
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 3 cut(s) 258, 2439, 2588
BsaBI GATNNNNATC 1 cut(s) 1079
BsaI GGTCTC 1 cut(s) 876
BsaJI CCNNGG 5 cut(s) 415, 475, 699, 2611, 2639
BsaWI WCCGGW 1 cut(s) 1660
Bsc4I CCNNNNNNNGG 2 cut(s) 587, 2212
Bse1I ACTGG 4 cut(s) 1451, 1496, 1853, 2604
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 2 cut(s) 835, 2697
Bse8I GATNNNNATC 1 cut(s) 1079
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 3 cut(s) 1058, 2721, 2739
BseDI CCNNGG 5 cut(s) 415, 475, 699, 2611, 2639
BseGI GGATG 4 cut(s) 156, 784, 967, 2596
BseJI GATNNNNATC 1 cut(s) 1079
BseLI CCNNNNNNNGG 2 cut(s) 587, 2212
BseMI GCAATG 2 cut(s) 835, 2697
BseMII CTCAG 4 cut(s) 684, 1427, 1550, 1988
BseNI ACTGG 4 cut(s) 1451, 1496, 1853, 2604
BseSI GKGCMC 2 cut(s) 79, 2586
BseXI GCAGC 8 cut(s) 278, 557, 1153, 2050, 2053, 2056, 2442, 2818
BsgI GTGCAG 1 cut(s) 2496
BshNI GGYRCC 1 cut(s) 1915
BsiHKAI GWGCWC 1 cut(s) 1056
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 2 cut(s) 1661, 2687
BslFI GGGAC 3 cut(s) 1206, 2188, 2460
BslI CCNNNNNNNGG 2 cut(s) 587, 2212
BsmAI GTCTC 2 cut(s) 876, 1682
BsmFI GGGAC 3 cut(s) 1206, 2188, 2460
BsmI GAATGC 1 cut(s) 365
Bso31I GGTCTC 1 cut(s) 876
BsoBI CYCGRG 1 cut(s) 476
Bsp120I GGGCCC 1 cut(s) 2582
Bsp1286I GDGCHC 5 cut(s) 79, 1056, 2207, 2364, 2586
Bsp13I TCCGGA 1 cut(s) 1660
Bsp1720I GCTNAGC 1 cut(s) 6
BspACI CCGC 1 cut(s) 1554
BspCNI CTCAG 4 cut(s) 685, 1428, 1549, 1987
BspEI TCCGGA 1 cut(s) 1660
BspLI GGNNCC 3 cut(s) 1881, 1917, 2584
BspT107I GGYRCC 1 cut(s) 1915
BspTI CTTAAG 1 cut(s) 2828
BspTNI GGTCTC 1 cut(s) 876
BsrDI GCAATG 2 cut(s) 835, 2697
BsrI ACTGG 4 cut(s) 1451, 1496, 1853, 2604
BssECI CCNNGG 5 cut(s) 415, 475, 699, 2611, 2639
BssT1I CCWWGG 4 cut(s) 415, 699, 2611, 2639
Bst2UI CCWGG 3 cut(s) 1058, 2721, 2739
Bst4CI ACNGT 6 cut(s) 15, 659, 720, 1161, 2627, 2866
Bst6I CTCTTC 2 cut(s) 372, 1797
BstAFI CTTAAG 1 cut(s) 2828
BstAPI GCANNNNNTGC 1 cut(s) 2399
BstC8I GCNNGC 6 cut(s) 55, 645, 1218, 2101, 2290, 2428
BstDEI CTNAG 9 cut(s) 6, 693, 890, 1436, 1536, 1784, 1974, 2114, 2795
BstEII GGTNACC 1 cut(s) 2750
BstF5I GGATG 4 cut(s) 156, 784, 967, 2596
BstH2I RGCGCY 2 cut(s) 272, 2100
BstHHI GCGC 5 cut(s) 271, 1938, 2022, 2099, 2677
BstMAI GTCTC 2 cut(s) 876, 1682
BstMWI GCNNNNNNNGC 6 cut(s) 617, 971, 1150, 2044, 2145, 2399
BstNI CCWGG 3 cut(s) 1058, 2721, 2739
BstNSI RCATGY 3 cut(s) 1239, 1469, 2029
BstPI GGTNACC 1 cut(s) 2750
BstSCI CCNGG 3 cut(s) 1056, 2719, 2737
BstSFI CTRYAG 1 cut(s) 391
BstSLI GKGCMC 2 cut(s) 79, 2586
BstV1I GCAGC 8 cut(s) 278, 557, 1153, 2050, 2053, 2056, 2442, 2818
BstV2I GAAGAC 3 cut(s) 1273, 1431, 2301
BstX2I RGATCY 4 cut(s) 481, 1101, 2734, 2857
BstYI RGATCY 4 cut(s) 481, 1101, 2734, 2857
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 2 cut(s) 850, 2727
BtgZI GCGATG 2 cut(s) 443, 635
BtsCI GGATG 4 cut(s) 156, 784, 967, 2596
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 5 cut(s) 77, 655, 808, 1393, 1489
Cac8I GCNNGC 6 cut(s) 55, 645, 1218, 2101, 2290, 2428
CfoI GCGC 5 cut(s) 271, 1938, 2022, 2099, 2677
Cfr13I GGNCC 7 cut(s) 1040, 1879, 2102, 2459, 2582, 2583, 2873
CseI GACGC 1 cut(s) 1203
Csp6I GTAC 7 cut(s) 124, 1070, 1085, 1224, 1916, 2548, 2623
CviQI GTAC 7 cut(s) 124, 1070, 1085, 1224, 1916, 2548, 2623
DdeI CTNAG 9 cut(s) 6, 693, 890, 1436, 1536, 1784, 1974, 2114, 2795
DraI TTTAAA 1 cut(s) 1875
EaeI YGGCCR 1 cut(s) 578
Eam1104I CTCTTC 2 cut(s) 372, 1797
EarI CTCTTC 2 cut(s) 372, 1797
Eco130I CCWWGG 4 cut(s) 415, 699, 2611, 2639
Eco147I AGGCCT 1 cut(s) 1828
Eco24I GRGCYC 3 cut(s) 2207, 2364, 2586
Eco31I GGTCTC 1 cut(s) 876
Eco32I GATATC 1 cut(s) 1658
Eco47I GGWCC 2 cut(s) 1879, 2873
Eco47III AGCGCT 1 cut(s) 2098
Eco57I CTGAAG 3 cut(s) 336, 2208, 2316
Eco81I CCTNAGG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 476
Eco91I GGTNACC 1 cut(s) 2750
EcoO109I RGGNCCY 2 cut(s) 1040, 1879
EcoO65I GGTNACC 1 cut(s) 2750
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 3 cut(s) 1056, 2719, 2737
EcoRV GATATC 1 cut(s) 1658
EcoT14I CCWWGG 4 cut(s) 415, 699, 2611, 2639
EcoT22I ATGCAT 3 cut(s) 967, 1471, 2697
EcoT38I GRGCYC 3 cut(s) 2207, 2364, 2586
ErhI CCWWGG 4 cut(s) 415, 699, 2611, 2639
FalI AAGNNNNNCTT 2 cut(s) 1803, 1835
FaqI GGGAC 3 cut(s) 1206, 2188, 2460
FbaI TGATCA 1 cut(s) 879
Fnu4HI GCNGC 9 cut(s) 267, 546, 1142, 1554, 2039, 2042, 2045, 2431, 2807
FokI GGATG 4 cut(s) 143, 771, 974, 2583
FriOI GRGCYC 3 cut(s) 2207, 2364, 2586
Fsp4HI GCNGC 9 cut(s) 267, 546, 1142, 1554, 2039, 2042, 2045, 2431, 2807
FspBI CTAG 8 cut(s) 489, 977, 1094, 1221, 1475, 1722, 1836, 2732
GlaI GCGC 5 cut(s) 270, 1937, 2021, 2098, 2676
GluI GCNGC 9 cut(s) 267, 546, 1142, 1554, 2039, 2042, 2045, 2431, 2807
GsuI CTGGAG 2 cut(s) 1040, 2834
HaeII RGCGCY 2 cut(s) 272, 2100
HapII CCGG 2 cut(s) 1661, 2687
HgaI GACGC 1 cut(s) 1203
HhaI GCGC 5 cut(s) 271, 1938, 2022, 2099, 2677
Hin6I GCGC 5 cut(s) 269, 1936, 2020, 2097, 2675
HinP1I GCGC 5 cut(s) 269, 1936, 2020, 2097, 2675
HindIII AAGCTT 2 cut(s) 212, 224
HpaII CCGG 2 cut(s) 1661, 2687
HphI GGTGA 3 cut(s) 644, 703, 1364
Hpy166II GTNNAC 2 cut(s) 2183, 2497
Hpy8I GTNNAC 2 cut(s) 2183, 2497
Hpy99I CGWCG 1 cut(s) 1020
HpyAV CCTTC 8 cut(s) 287, 311, 1253, 1400, 1508, 1929, 2340, 2688
HpyCH4III ACNGT 6 cut(s) 15, 659, 720, 1161, 2627, 2866
HpyCH4IV ACGT 4 cut(s) 1018, 1304, 1840, 1946
HpyF10VI GCNNNNNNNGC 6 cut(s) 617, 971, 1150, 2044, 2145, 2399
HpyF3I CTNAG 9 cut(s) 6, 693, 890, 1436, 1536, 1784, 1974, 2114, 2795
HpySE526I ACGT 4 cut(s) 1018, 1304, 1840, 1946
HspAI GCGC 5 cut(s) 269, 1936, 2020, 2097, 2675
Kpn2I TCCGGA 1 cut(s) 1660
KpnI GGTACC 1 cut(s) 1919
Ksp22I TGATCA 1 cut(s) 879
LmnI GCTCC 3 cut(s) 1059, 1150, 2326
Lsp1109I GCAGC 8 cut(s) 278, 557, 1153, 2050, 2053, 2056, 2442, 2818
LweI GCATC 5 cut(s) 423, 950, 952, 1540, 2581
MaeI CTAG 8 cut(s) 489, 977, 1094, 1221, 1475, 1722, 1836, 2732
MaeII ACGT 4 cut(s) 1018, 1304, 1840, 1946
MaeIII GTNAC 6 cut(s) 1947, 2053, 2252, 2606, 2750, 2785
MfeI CAATTG 2 cut(s) 1376, 2887
MflI RGATCY 4 cut(s) 481, 1101, 2734, 2857
MhlI GDGCHC 5 cut(s) 79, 1056, 2207, 2364, 2586
MlsI TGGCCA 1 cut(s) 580
MluNI TGGCCA 1 cut(s) 580
MlyI GAGTC 8 cut(s) 389, 1016, 1112, 1655, 1682, 1966, 2002, 2699
MmeI TCCRAC 2 cut(s) 939, 2885
Mox20I TGGCCA 1 cut(s) 580
Mph1103I ATGCAT 3 cut(s) 967, 1471, 2697
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 4 cut(s) 321, 1815, 2524, 2538
MscI TGGCCA 1 cut(s) 580
MseI TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1560, 1874, 2373, 2829
MslI CAYNNNNRTG 5 cut(s) 108, 1187, 2533, 2577, 2700
Msp20I TGGCCA 1 cut(s) 580
MspA1I CMGCKG 3 cut(s) 70, 178, 1253
MspCI CTTAAG 1 cut(s) 2828
MspI CCGG 2 cut(s) 1661, 2687
MspR9I CCNGG 3 cut(s) 1058, 2721, 2739
MunI CAATTG 2 cut(s) 1376, 2887
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 3 cut(s) 1058, 2721, 2739
MwoI GCNNNNNNNGC 6 cut(s) 617, 971, 1150, 2044, 2145, 2399
NlaIV GGNNCC 3 cut(s) 1881, 1917, 2584
NmuCI GTSAC 4 cut(s) 1947, 2053, 2252, 2785
NsiI ATGCAT 3 cut(s) 967, 1471, 2697
NspI RCATGY 3 cut(s) 1239, 1469, 2029
PceI AGGCCT 1 cut(s) 1828
PciI ACATGT 1 cut(s) 2025
PcsI WCGNNNNNNNCGW 1 cut(s) 1537
PctI GAATGC 1 cut(s) 365
PdmI GAANNNNTTC 4 cut(s) 321, 1815, 2524, 2538
PfeI GAWTC 6 cut(s) 64, 1025, 1090, 1583, 2228, 2264
PflFI GACNNNGTC 1 cut(s) 1645
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 9 cut(s) 268, 547, 1143, 1555, 2040, 2043, 2046, 2432, 2808
PleI GAGTC 8 cut(s) 388, 1015, 1112, 1654, 1681, 1966, 2002, 2699
PpsI GAGTC 8 cut(s) 388, 1015, 1112, 1654, 1681, 1966, 2002, 2699
PpuMI RGGWCCY 1 cut(s) 1879
PscI ACATGT 1 cut(s) 2025
Psp5II RGGWCCY 1 cut(s) 1879
Psp6I CCWGG 3 cut(s) 1056, 2719, 2737
PspEI GGTNACC 1 cut(s) 2750
PspGI CCWGG 3 cut(s) 1056, 2719, 2737
PspN4I GGNNCC 3 cut(s) 1881, 1917, 2584
PspOMI GGGCCC 1 cut(s) 2582
PspPI GGNCC 7 cut(s) 1040, 1879, 2102, 2459, 2582, 2583, 2873
PspPPI RGGWCCY 1 cut(s) 1879
PsuI RGATCY 4 cut(s) 481, 1101, 2734, 2857
PsyI GACNNNGTC 1 cut(s) 1645
PvuII CAGCTG 3 cut(s) 70, 178, 1253
RsaI GTAC 7 cut(s) 125, 1071, 1086, 1225, 1917, 2549, 2624
RsaNI GTAC 7 cut(s) 124, 1070, 1085, 1224, 1916, 2548, 2623
RseI CAYNNNNRTG 5 cut(s) 108, 1187, 2533, 2577, 2700
SaqAI TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1560, 1874, 2373, 2829
SatI GCNGC 9 cut(s) 267, 546, 1142, 1554, 2039, 2042, 2045, 2431, 2807
Sau96I GGNCC 7 cut(s) 1040, 1879, 2102, 2459, 2582, 2583, 2873
SchI GAGTC 8 cut(s) 389, 1016, 1112, 1655, 1682, 1966, 2002, 2699
ScrFI CCNGG 3 cut(s) 1058, 2721, 2739
SduI GDGCHC 5 cut(s) 79, 1056, 2207, 2364, 2586
SfaNI GCATC 5 cut(s) 423, 950, 952, 1540, 2581
SfcI CTRYAG 1 cut(s) 391
SinI GGWCC 2 cut(s) 1879, 2873
SmiMI CAYNNNNRTG 5 cut(s) 108, 1187, 2533, 2577, 2700
SmlI CTYRAG 4 cut(s) 273, 2454, 2567, 2828
SmoI CTYRAG 4 cut(s) 273, 2454, 2567, 2828
SseBI AGGCCT 1 cut(s) 1828
SsiI CCGC 1 cut(s) 1554
SspMI CTAG 8 cut(s) 489, 977, 1094, 1221, 1475, 1722, 1836, 2732
StuI AGGCCT 1 cut(s) 1828
StyD4I CCNGG 3 cut(s) 1056, 2719, 2737
StyI CCWWGG 4 cut(s) 415, 699, 2611, 2639
TaaI ACNGT 6 cut(s) 15, 659, 720, 1161, 2627, 2866
TaiI ACGT 4 cut(s) 1021, 1307, 1843, 1949
TaqI TCGA 4 cut(s) 1015, 1048, 1121, 2708
TatI WGTACW 3 cut(s) 1069, 1223, 2547
TauI GCSGC 1 cut(s) 1556
TfiI GAWTC 6 cut(s) 64, 1025, 1090, 1583, 2228, 2264
Tru1I TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1560, 1874, 2373, 2829
Tru9I TTAA 9 cut(s) 299, 678, 774, 905, 1098, 1560, 1874, 2373, 2829
TscAI CASTG 5 cut(s) 84, 662, 808, 1393, 1496
TseFI GTSAC 4 cut(s) 1947, 2053, 2252, 2785
TseI GCWGC 8 cut(s) 266, 545, 1141, 2038, 2041, 2044, 2430, 2806
Tsp45I GTSAC 4 cut(s) 1947, 2053, 2252, 2785
TspGWI ACGGA 1 cut(s) 2295
TspRI CASTG 5 cut(s) 84, 662, 808, 1393, 1496
Tth111I GACNNNGTC 1 cut(s) 1645
Van91I CCANNNNNTGG 1 cut(s) 587
Vha464I CTTAAG 1 cut(s) 2828
VpaK11BI GGWCC 2 cut(s) 1879, 2873
XapI RAATTY 8 cut(s) 236, 321, 337, 573, 862, 1355, 1622, 2282
XbaI TCTAGA 1 cut(s) 2731
XceI RCATGY 3 cut(s) 1239, 1469, 2029
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XmnI GAANNNNTTC 4 cut(s) 321, 1815, 2524, 2538
XspI CTAG 8 cut(s) 489, 977, 1094, 1221, 1475, 1722, 1836, 2732
Zsp2I ATGCAT 3 cut(s) 967, 1471, 2697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.