AT1G32550

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009435
Physical Location & Seq
Reverse (-)
11771621 .. 11774294
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32550.1

Sequence Viewer

Length: 546 bp
ATGGCTCTGATTTTGCCCTGTACATTTTGTACTTCGCTTCAGAAGAAGAATTTTCCGATTAATCGGAGATATATTACGAATTTCCGTCGAGGAGCTACGACTGCGACGTGTGAGTTCCGGATTCCTGTAGAAGTTTCCACTCCATCAGATAGAGGATCGTTGGTTGTTCCTTCGCACAAAGTCACTGTTCACGATCGACAACGAGGAGTGGTTCACGAATTCGAGGTTCCAGAGGATCAGTATATATTGCATTCAGCTGAATCTCAGAACATTAGTCTTCCGTTTGCTTGCAGGCATGGTTGTTGTACTAGTTGTGCTGTACGTGTAAAATCTGGAGAGCTGAGGCAGCCTCAAGCATTGGGAATATCAGCAGAACTGAAGTCCCAGGGGTATGCACTTCTTTGTGTGGGTTTCCCCACATCTGACCTTGAAGTAGAAACACAAGACGAGGACGAGGTCTACTGGCTACAATTTGGAAGATACTTTGCTCGTGGACCAATTGAAAGAGACGATTACGCTCTTGAACTCGCCATGGGAGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 map00195
Pfam Domains
Protein Families
Fer2

Protein Analysis

181

Amino Acids

20.38

Weight (kDa)

5.57

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 69 - 139 1e-15 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 459
AccIII TCCGGA 1 cut(s) 117
AclWI GGATC 2 cut(s) 163, 243
AcsI RAATTY 3 cut(s) 49, 79, 218
AcuI CTGAAG 2 cut(s) 23, 398
AfaI GTAC 4 cut(s) 22, 31, 307, 321
AflIII ACRYGT 2 cut(s) 107, 322
AgsI TTSAA 3 cut(s) 431, 503, 524
AhlI ACTAGT 1 cut(s) 308
AjiI CACGTC 1 cut(s) 108
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 3 cut(s) 95, 257, 340
AluI AGCT 3 cut(s) 95, 257, 340
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 2 cut(s) 163, 243
Aor13HI TCCGGA 1 cut(s) 117
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 3 cut(s) 49, 79, 218
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 494
AvaII GGWCC 1 cut(s) 494
BauI CACGAG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 269
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 501
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 126
BisI GCNGC 1 cut(s) 347
BlsI GCNGC 1 cut(s) 348
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 386
BpiI GAAGAC 1 cut(s) 269
BplI GAGNNNNNCTC 2 cut(s) 334, 366
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 384, 385, 531
BsaWI WCCGGW 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 467
BseAI TCCGGA 1 cut(s) 117
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 384, 385, 531
BseMII CTCAG 2 cut(s) 278, 332
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 2 cut(s) 105, 219
BseXI GCAGC 1 cut(s) 358
Bsh1285I CGRYCG 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 501
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 250
Bsp13I TCCGGA 1 cut(s) 117
Bsp1407I TGTACA 1 cut(s) 20
Bsp143I GATC 3 cut(s) 155, 193, 235
Bsp19I CCATGG 1 cut(s) 531
BspCNI CTCAG 2 cut(s) 277, 333
BspEI TCCGGA 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 2 cut(s) 163, 243
BsrGI TGTACA 1 cut(s) 20
BsrI ACTGG 1 cut(s) 467
BssECI CCNNGG 3 cut(s) 384, 385, 531
BssMI GATC 3 cut(s) 155, 193, 235
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 1 cut(s) 531
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 289, 293
BstDEI CTNAG 2 cut(s) 264, 341
BstDSI CCRYGG 1 cut(s) 531
BstKTI GATC 3 cut(s) 158, 196, 238
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 3 cut(s) 155, 193, 235
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 101, 346
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 269
BtgI CCRYGG 1 cut(s) 531
BtrI CACGTC 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 289, 293
Cfr13I GGNCC 1 cut(s) 494
Csp6I GTAC 4 cut(s) 21, 30, 306, 320
CviAII CATG 2 cut(s) 296, 532
CviJI RGCY 6 cut(s) 5, 95, 257, 340, 349, 466
CviKI_1 RGCY 6 cut(s) 5, 95, 257, 340, 349, 466
CviQI GTAC 4 cut(s) 21, 30, 306, 320
DdeI CTNAG 2 cut(s) 264, 341
DpnI GATC 3 cut(s) 157, 195, 237
DpnII GATC 3 cut(s) 155, 193, 235
Eco130I CCWWGG 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 494
Eco57I CTGAAG 2 cut(s) 23, 398
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 2 cut(s) 299, 535
FaiI YATR 6 cut(s) 72, 243, 245, 297, 393, 533
FaqI GGGAC 1 cut(s) 367
FatI CATG 2 cut(s) 295, 531
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 347
FspBI CTAG 1 cut(s) 309
GluI GCNGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 354
HapII CCGG 1 cut(s) 118
Hin1II CATG 2 cut(s) 299, 535
HinfI GANTC 2 cut(s) 121, 260
HpaII CCGG 1 cut(s) 118
Hpy166II GTNNAC 4 cut(s) 190, 214, 460, 494
Hpy188I TCNGA 7 cut(s) 9, 42, 57, 66, 148, 267, 424
Hpy188III TCNNGA 6 cut(s) 118, 191, 215, 230, 333, 521
Hpy8I GTNNAC 4 cut(s) 190, 214, 460, 494
Hpy99I CGWCG 2 cut(s) 90, 109
HpyAV CCTTC 1 cut(s) 180
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 107, 322
HpyCH4V TGCA 3 cut(s) 250, 291, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 346
HpyF3I CTNAG 2 cut(s) 264, 341
HpySE526I ACGT 2 cut(s) 107, 322
Hsp92II CATG 2 cut(s) 299, 535
Kpn2I TCCGGA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 155, 193, 235
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 9 cut(s) 31, 131, 138, 243, 277, 318, 371, 398, 448
Lsp1109I GCAGC 1 cut(s) 358
MaeI CTAG 1 cut(s) 309
MaeII ACGT 2 cut(s) 107, 322
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 157, 195, 237
MboI GATC 3 cut(s) 155, 193, 235
MboII GAAGA 4 cut(s) 55, 58, 269, 489
MfeI CAATTG 1 cut(s) 498
MluCI AATT 5 cut(s) 49, 79, 218, 470, 498
MnlI CCTC 9 cut(s) 83, 146, 197, 217, 226, 336, 360, 442, 448
MroI TCCGGA 1 cut(s) 117
MseI TTAA 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 1 cut(s) 250
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 101, 346
NcoI CCATGG 1 cut(s) 531
NdeII GATC 3 cut(s) 155, 193, 235
NlaIII CATG 2 cut(s) 299, 535
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 181
PasI CCCWGGG 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PctI GAATGC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 121, 260
PflFI GACNNNGTC 1 cut(s) 455
PkrI GCNGC 1 cut(s) 348
Ple19I CGATCG 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 323
PshBI ATTAAT 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 494
PsyI GACNNNGTC 1 cut(s) 455
PvuI CGATCG 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 4 cut(s) 22, 31, 307, 321
RsaNI GTAC 4 cut(s) 21, 30, 306, 320
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 1 cut(s) 347
Sau3AI GATC 3 cut(s) 155, 193, 235
Sau96I GGNCC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 386
SetI ASST 8 cut(s) 97, 110, 228, 259, 325, 342, 429, 459
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 308
Sse9I AATT 5 cut(s) 49, 79, 218, 470, 498
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 2 cut(s) 110, 325
TaqI TCGA 3 cut(s) 88, 196, 222
TasI AATT 5 cut(s) 49, 79, 218, 470, 498
TatI WGTACW 3 cut(s) 20, 29, 305
TfiI GAWTC 2 cut(s) 121, 260
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 181
TspGWI ACGGA 2 cut(s) 74, 270
TspRI CASTG 1 cut(s) 190
Tth111I GACNNNGTC 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 494
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 3 cut(s) 49, 79, 218
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.