AT1G33230

Transmembrane protein 120 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006071
Physical Location & Seq
Forward (+)
12046379 .. 12050095
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33230.1

Sequence Viewer

Length: 1044 bp
ATGGAAGAGGTTGAAGAAGAAGTGAGACGAATCGTCGAACAAGTTAAAGAGCTTCATGACTCTTCCACATCCTTCGTCTCTTCTTCTTCTCAAGAAGAGCTTTCTCTCCGTAACAGAGCCTCCGTCGTTGACTCATCAATCCGCCGTCTTCATTCTACTCTTGCCTCTGACAAACATCTCGATCCCAAGCTTTTCGAAAAGCTAGAAGAGGATCTGCAACGAGCTAGATGCATGCTTGCAGATGGAGACACTTCATCGTTTCTTCCATCTAAACCTCAAGGACGTTTTGTGAGAATGTTTTTGGGACCTGTGAATGTTAGAGCTTCACGAAAAGATATACAGCTTAAAGTTAAAGAAGAATATAATAGCTACAGAGATAGGACAGCTCTTCTTTTTCTTGTTTTCCCTGCAATACTTTTGACACTAAGATCTTATGTTTGGGATGGATGTTTGCCTGCATTCCCTGTTCAGCTCTACCAGGCTTGGCTACTGTTTCTTTATGCTGGTTTGGTTATGCGAGAGAACATTTTAAGAGCTAATGGGAGTGATATCCGTTCATGGTGGCTTTATCATCATTATTTTGCAATGGCTATGTCCCTTGTCAGTCTCACTTGGGAAATCAAAGGTCAACCAAACTGCGTTCAGAAGCAGAAAGGAGTGCGTCTTTTCCTCCAATGGGCTATGATGCAAGGTGTCGCGATGCTTCTGCAAAATAGATATCAGCGCCAAAGATTATACACTCGCATTGCACTTGGAAAGGCTAAAAGGATGGACGTCGTATGGGGAGAAACGGCTGGAGTAGATGGGCAACTATTGCTATTATTTCCTATTTTGTTCACTTTACAGGGCTTTGAAGCATACGTGGGATTCCAGTTGCTTAGGACAGTGTTGATGGGGGTAGTCGCTGAATGGCAGGTATTGGTTTGTGGCATTCTTCTGGTTGTGATGGCCATTGGAAACTTCATAAACACAGTAGAGACACTAATGGTGAAGTCGAGATTCAAGGCGAAGATGAAGAGAAGTAAAAGCAGAGCAGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

347

Amino Acids

40.01

Weight (kDa)

9.58

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 5 - 334 6.7e-97 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 777
Acc36I ACCTGC 1 cut(s) 904
AccII CGCG 1 cut(s) 698
AciI CCGC 1 cut(s) 142
AclWI GGATC 2 cut(s) 176, 219
AcoI YGGCCR 1 cut(s) 948
AcyI GRCGYC 1 cut(s) 774
AfiI CCNNNNNNNGG 1 cut(s) 676
AgsI TTSAA 3 cut(s) 14, 854, 1003
AhdI GACNNNNNGTC 1 cut(s) 32
AjnI CCWGG 1 cut(s) 477
Alw26I GTCTC 5 cut(s) 19, 82, 240, 611, 971
AlwI GGATC 2 cut(s) 176, 219
AoxI GGCC 1 cut(s) 948
AspLEI GCGC 1 cut(s) 726
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 1000
AsuII TTCGAA 1 cut(s) 195
AvaII GGWCC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 950
BbsI GAAGAC 1 cut(s) 140
BccI CCATC 7 cut(s) 236, 274, 437, 763, 797, 886, 940
BceAI ACGGC 2 cut(s) 129, 807
BcgI CGANNNNNNTGC 4 cut(s) 210, 244, 688, 722
BciT130I CCWGG 1 cut(s) 479
BcoDI GTCTC 5 cut(s) 19, 82, 240, 611, 971
BfaI CTAG 2 cut(s) 203, 225
BfmI CTRYAG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 727
BfuAI ACCTGC 1 cut(s) 904
BglII AGATCT 1 cut(s) 428
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 305
BmeRI GACNNNNNGTC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 3 cut(s) 218, 675, 690
BpiI GAAGAC 1 cut(s) 140
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 878
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 75, 261
BsaAI YACGTR 1 cut(s) 862
BsaHI GRCGYC 1 cut(s) 774
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 871
Bse3DI GCAATG 2 cut(s) 591, 744
BseBI CCWGG 1 cut(s) 479
BseGI GGATG 4 cut(s) 68, 448, 452, 774
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 2 cut(s) 591, 744
BseNI ACTGG 1 cut(s) 871
Bsh1236I CGCG 1 cut(s) 698
BshFI GGCC 1 cut(s) 950
BslFI GGGAC 2 cut(s) 318, 580
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 5 cut(s) 19, 82, 240, 611, 971
BsmBI CGTCTC 2 cut(s) 19, 82
BsmFI GGGAC 2 cut(s) 318, 580
BsmI GAATGC 2 cut(s) 458, 930
BsnI GGCC 1 cut(s) 950
Bsp119I TTCGAA 1 cut(s) 195
Bsp143I GATC 3 cut(s) 181, 211, 428
Bsp68I TCGCGA 1 cut(s) 698
BspACI CCGC 1 cut(s) 142
BspANI GGCC 1 cut(s) 950
BspFNI CGCG 1 cut(s) 698
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 306
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 2 cut(s) 176, 219
BspQI GCTCTTC 2 cut(s) 90, 393
BspT104I TTCGAA 1 cut(s) 195
BsrDI GCAATG 2 cut(s) 591, 744
BsrI ACTGG 1 cut(s) 871
BssMI GATC 3 cut(s) 181, 211, 428
BssNI GRCGYC 1 cut(s) 774
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 492, 886, 973
Bst6I CTCTTC 6 cut(s) 67, 85, 90, 201, 393, 1010
BstACI GRCGYC 1 cut(s) 774
BstAPI GCANNNNNTGC 1 cut(s) 814
BstBAI YACGTR 1 cut(s) 862
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 3 cut(s) 233, 237, 456
BstDEI CTNAG 2 cut(s) 425, 878
BstF5I GGATG 4 cut(s) 68, 448, 452, 774
BstFNI CGCG 1 cut(s) 698
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 1 cut(s) 726
BstKTI GATC 3 cut(s) 184, 214, 431
BstMAI GTCTC 5 cut(s) 19, 82, 240, 611, 971
BstMBI GATC 3 cut(s) 181, 211, 428
BstMWI GCNNNNNNNGC 1 cut(s) 814
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 370
BstUI CGCG 1 cut(s) 698
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 2 cut(s) 211, 428
BstYI RGATCY 2 cut(s) 211, 428
BsuRI GGCC 1 cut(s) 950
BtgZI GCGATG 1 cut(s) 713
BtsCI GGATG 4 cut(s) 68, 448, 452, 774
BtsIMutI CAGTG 1 cut(s) 891
BtuMI TCGCGA 1 cut(s) 698
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 3 cut(s) 233, 237, 456
CciI TCATGA 1 cut(s) 55
CfoI GCGC 1 cut(s) 726
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 650
CviAII CATG 3 cut(s) 56, 232, 558
DdeI CTNAG 2 cut(s) 425, 878
DpnI GATC 3 cut(s) 183, 213, 430
DpnII GATC 3 cut(s) 181, 211, 428
DriI GACNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 948
Eam1104I CTCTTC 6 cut(s) 67, 85, 90, 201, 393, 1010
Eam1105I GACNNNNNGTC 1 cut(s) 32
EarI CTCTTC 6 cut(s) 67, 85, 90, 201, 393, 1010
EciI GGCGGA 1 cut(s) 131
Eco32I GATATC 2 cut(s) 550, 719
Eco47I GGWCC 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 477
EcoRV GATATC 2 cut(s) 550, 719
EcoT22I ATGCAT 1 cut(s) 233
Esp3I CGTCTC 2 cut(s) 19, 82
FaeI CATG 3 cut(s) 59, 235, 561
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 2 cut(s) 318, 580
FatI CATG 3 cut(s) 55, 231, 557
FokI GGATG 4 cut(s) 55, 455, 459, 781
FspBI CTAG 2 cut(s) 203, 225
GlaI GCGC 1 cut(s) 725
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 1 cut(s) 950
HgaI GACGC 1 cut(s) 650
HhaI GCGC 1 cut(s) 726
Hin1I GRCGYC 1 cut(s) 774
Hin1II CATG 3 cut(s) 59, 235, 561
Hin6I GCGC 1 cut(s) 724
HinP1I GCGC 1 cut(s) 724
HincII GTYRAC 2 cut(s) 130, 629
HindII GTYRAC 2 cut(s) 130, 629
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 5 cut(s) 30, 59, 131, 867, 999
HphI GGTGA 1 cut(s) 1000
Hpy166II GTNNAC 3 cut(s) 130, 629, 837
Hpy188I TCNGA 2 cut(s) 169, 645
Hpy188III TCNNGA 6 cut(s) 56, 92, 179, 327, 697, 996
Hpy8I GTNNAC 3 cut(s) 130, 629, 837
Hpy99I CGWCG 3 cut(s) 38, 128, 779
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 492, 886, 973
HpyCH4IV ACGT 3 cut(s) 283, 774, 861
HpyCH4V TGCA 9 cut(s) 217, 231, 239, 410, 458, 584, 688, 709, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 814
HpyF3I CTNAG 2 cut(s) 425, 878
HpySE526I ACGT 3 cut(s) 283, 774, 861
Hsp92I GRCGYC 1 cut(s) 774
Hsp92II CATG 3 cut(s) 59, 235, 561
HspAI GCGC 1 cut(s) 724
Kzo9I GATC 3 cut(s) 181, 211, 428
LguI GCTCTTC 2 cut(s) 90, 393
LweI GCATC 3 cut(s) 218, 675, 690
MaeI CTAG 2 cut(s) 203, 225
MaeII ACGT 3 cut(s) 283, 774, 861
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 183, 213, 430
MboI GATC 3 cut(s) 181, 211, 428
MflI RGATCY 2 cut(s) 211, 428
MlsI TGGCCA 1 cut(s) 950
MluNI TGGCCA 1 cut(s) 950
MlyI GAGTC 2 cut(s) 53, 125
MnlI CCTC 5 cut(s) 130, 175, 202, 285, 680
Mox20I TGGCCA 1 cut(s) 950
Mph1103I ATGCAT 1 cut(s) 233
MscI TGGCCA 1 cut(s) 950
MseI TTAA 4 cut(s) 45, 345, 351, 530
Msp20I TGGCCA 1 cut(s) 950
MspR9I CCNGG 1 cut(s) 479
Mva1269I GAATGC 2 cut(s) 458, 930
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 698
MwoI GCNNNNNNNGC 1 cut(s) 814
NdeII GATC 3 cut(s) 181, 211, 428
NlaIII CATG 3 cut(s) 59, 235, 561
NlaIV GGNNCC 1 cut(s) 306
NruI TCGCGA 1 cut(s) 698
NsiI ATGCAT 1 cut(s) 233
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 1 cut(s) 195
PaeI GCATGC 1 cut(s) 235
PagI TCATGA 1 cut(s) 55
PciSI GCTCTTC 2 cut(s) 90, 393
PctI GAATGC 2 cut(s) 458, 930
PfeI GAWTC 3 cut(s) 30, 867, 999
PleI GAGTC 2 cut(s) 53, 125
PpsI GAGTC 2 cut(s) 53, 125
Ppu21I YACGTR 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 305
PspPPI RGGWCCY 1 cut(s) 305
PsuI RGATCY 2 cut(s) 211, 428
RruI TCGCGA 1 cut(s) 698
SapI GCTCTTC 2 cut(s) 90, 393
SaqAI TTAA 4 cut(s) 45, 345, 351, 530
Sau3AI GATC 3 cut(s) 181, 211, 428
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 53, 125
ScrFI CCNGG 1 cut(s) 479
SfaNI GCATC 3 cut(s) 218, 675, 690
SfcI CTRYAG 1 cut(s) 370
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 90, 276
SmoI CTYRAG 2 cut(s) 90, 276
SphI GCATGC 1 cut(s) 235
SsiI CCGC 1 cut(s) 142
SspMI CTAG 2 cut(s) 203, 225
StyD4I CCNGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 492, 886, 973
TaiI ACGT 3 cut(s) 286, 777, 864
TaqI TCGA 4 cut(s) 36, 180, 195, 995
TfiI GAWTC 3 cut(s) 30, 867, 999
Tru1I TTAA 4 cut(s) 45, 345, 351, 530
Tru9I TTAA 4 cut(s) 45, 345, 351, 530
TscAI CASTG 1 cut(s) 891
TspDTI ATGAA 6 cut(s) 44, 140, 243, 546, 952, 1028
TspGWI ACGGA 3 cut(s) 98, 112, 542
TspRI CASTG 1 cut(s) 891
VpaK11BI GGWCC 1 cut(s) 305
XceI RCATGY 1 cut(s) 235
XspI CTAG 2 cut(s) 203, 225
ZraI GACGTC 1 cut(s) 775
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.