MD14G1143500.v1.1

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006071
Physical Location & Seq
Forward (+)
23612311 .. 23616858
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1143500.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGGGGATGCCAAGAGGAACACTTCTTCTTCTTCTTCCGGCAGTGACGAGGAGATACAAGCACAAGTGGCCAAGGCGGTGGAGGAGGTGAAGGAGCTGCAGGACAAAGCTGCTTCCTTTGTATCCAGAGCCGCCACCGACGAGCAATCCCTCCGCCAGCGCGCCCAATCTCTCGATTCCTCCATCCGCCGCCTCCGCTCCCAGCTTTATTCCCTGCTTTCCAGCAAGGCCTTGGATCCCAAGTTAGCAGAAAAGCTGGAAGAAGATTTGCAGAGGGCCACATGTGTGTTGGTGGATGGCGAGGCCGCTTCATTTCTTCCTGGGAAATCTCAGGGAGGGTTTCTGAGGATGTTTCTCGGTCCTATCAATGTACGTTCCTCTAGCAAAGATATCCAACTCAAGGTTAAAGAGGAATACAACGCTTACAGAGATAGAACTGCCCTGCTATTCCTTATTTTTCCAGCAACACTACTAATACTAAGATCTTGGATTTGGGATGGATGTTTGCCAGCATTTCCAGTTCAGGCTTACCAGGCATGGCTGTTATTCCTCTACACTGGTTTGGCCCTGCGAGAAAACATACTGAGAGTAAATGGAAGTGATATTCGTCCATGGTGGATATACCATCACTATTGTGCTATGTTTATGGCCCTTGTTAGTCTCACATGGGAGATTAAAGGACAACCAAATTGTTCCCAAAAGCAGAGAGGTGTGCAACTTTTCCTACAATGGGCTATGATGCAAGGAGTTGCTATGCTTTTACAAAATAGATATCAACGCCAGAGACTCTATACTCGAATTGCATTGGGAAAGGCCAAAAGAATGGATGTGGTCTGGGGAGAAACAGCAGGCGTAGATGGTCAATTGTGGATTCTATGTCCAATACTATTTGTTTTACAGGGTTTTGAGGCATATGTCGGAATACAGTTGCTCACGACTGCTTTTATTGGCGTCGTTTCGGAATGGCAGGTGAGTTTTTGTGGCGTGCTGTTAGTTTTAATGGCAGTTGGGAACTTTGTAAATACGGTACAGACGCTTATGGTGAAGTCGAGGTTTAAGGCAAAGATGAAAAGAAGTAAGAGCAAGCAGGATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

366

Amino Acids

41.3

Weight (kDa)

9.5

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 21 - 352 6e-100 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 958
Acc36I ACCTGC 1 cut(s) 958
AccBSI CCGCTC 1 cut(s) 198
AccII CGCG 1 cut(s) 162
AciI CCGC 7 cut(s) 77, 132, 154, 187, 190, 196, 306
AclWI GGATC 2 cut(s) 230, 243
AcoI YGGCCR 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 951
AfaI GTAC 2 cut(s) 372, 1029
AfiI CCNNNNNNNGG 2 cut(s) 400, 730
AflIII ACRYGT 1 cut(s) 281
AjnI CCWGG 2 cut(s) 319, 531
AleI CACNNNNGTG 2 cut(s) 284, 633
AluBI AGCT 4 cut(s) 97, 110, 205, 256
AluI AGCT 4 cut(s) 97, 110, 205, 256
Alw26I GTCTC 2 cut(s) 665, 778
AlwI GGATC 2 cut(s) 230, 243
AlwNI CAGNNNCTG 1 cut(s) 1093
AoxI GGCC 7 cut(s) 69, 228, 276, 303, 564, 648, 813
ApeKI GCWGC 2 cut(s) 97, 110
AspLEI GCGC 2 cut(s) 162, 164
AspS9I GGNCC 4 cut(s) 276, 359, 565, 649
AsuHPI GGTGA 3 cut(s) 100, 982, 1054
AvaII GGWCC 1 cut(s) 359
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 235
BbvI GCAGC 2 cut(s) 84, 97
BccI CCATC 5 cut(s) 191, 290, 491, 633, 851
BciT130I CCWGG 2 cut(s) 321, 533
BciVI GTATCC 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 665, 778
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 2 cut(s) 98, 1094
BfuAI ACCTGC 1 cut(s) 958
BfuI GTATCC 1 cut(s) 133
BglII AGATCT 1 cut(s) 482
BisI GCNGC 5 cut(s) 98, 111, 132, 190, 306
BlsI GCNGC 5 cut(s) 99, 112, 133, 191, 307
Bme1390I CCNGG 2 cut(s) 321, 533
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 4 cut(s) 276, 359, 565, 649
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 2 cut(s) 321, 533
BmsI GCATC 1 cut(s) 729
BpuEI CTTGAG 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 951
BsaJI CCNNGG 4 cut(s) 72, 231, 320, 611
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 730
Bse1I ACTGG 2 cut(s) 518, 562
BseBI CCWGG 2 cut(s) 321, 533
BseDI CCNNGG 4 cut(s) 72, 231, 320, 611
BseGI GGATG 7 cut(s) 13, 183, 301, 354, 502, 506, 832
BseLI CCNNNNNNNGG 2 cut(s) 400, 730
BseMII CTCAG 3 cut(s) 335, 344, 575
BseNI ACTGG 2 cut(s) 518, 562
BsePI GCGCGC 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 65, 98
BseXI GCAGC 2 cut(s) 84, 97
BseYI CCCAGC 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 7 cut(s) 71, 230, 278, 305, 566, 650, 815
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 400, 730
BsmAI GTCTC 2 cut(s) 665, 778
BsnI GGCC 7 cut(s) 71, 230, 278, 305, 566, 650, 815
Bsp143I GATC 3 cut(s) 235, 482, 1090
Bsp19I CCATGG 1 cut(s) 611
BspACI CCGC 7 cut(s) 77, 132, 154, 187, 190, 196, 306
BspANI GGCC 7 cut(s) 71, 230, 278, 305, 566, 650, 815
BspCNI CTCAG 3 cut(s) 336, 343, 576
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 958
BspPI GGATC 2 cut(s) 230, 243
BsrBI CCGCTC 1 cut(s) 198
BsrI ACTGG 2 cut(s) 518, 562
BssECI CCNNGG 4 cut(s) 72, 231, 320, 611
BssHII GCGCGC 1 cut(s) 160
BssMI GATC 3 cut(s) 235, 482, 1090
BssNI GRCGYC 1 cut(s) 951
BssT1I CCWWGG 3 cut(s) 72, 231, 611
Bst2UI CCWGG 2 cut(s) 321, 533
Bst4CI ACNGT 2 cut(s) 927, 1027
BstACI GRCGYC 1 cut(s) 951
BstC8I GCNNGC 6 cut(s) 158, 162, 510, 850, 986, 1085
BstDEI CTNAG 4 cut(s) 330, 344, 479, 584
BstDSI CCRYGG 1 cut(s) 611
BstF5I GGATG 7 cut(s) 13, 183, 301, 354, 502, 506, 832
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 162, 164
BstKTI GATC 3 cut(s) 238, 485, 1093
BstMAI GTCTC 2 cut(s) 665, 778
BstMBI GATC 3 cut(s) 235, 482, 1090
BstMWI GCNNNNNNNGC 3 cut(s) 68, 195, 533
BstNI CCWGG 2 cut(s) 321, 533
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 319, 531
BstSFI CTRYAG 2 cut(s) 98, 1094
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 84, 97
BstX2I RGATCY 3 cut(s) 235, 482, 1090
BstXI CCANNNNNNTGG 2 cut(s) 79, 823
BstYI RGATCY 3 cut(s) 235, 482, 1090
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 7 cut(s) 71, 230, 278, 305, 566, 650, 815
BtgI CCRYGG 1 cut(s) 611
BtsCI GGATG 7 cut(s) 13, 183, 301, 354, 502, 506, 832
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 555
BveI ACCTGC 1 cut(s) 958
Cac8I GCNNGC 6 cut(s) 158, 162, 510, 850, 986, 1085
CaiI CAGNNNCTG 1 cut(s) 1093
CfoI GCGC 2 cut(s) 162, 164
Cfr13I GGNCC 4 cut(s) 276, 359, 565, 649
CseI GACGC 2 cut(s) 940, 1042
Csp6I GTAC 2 cut(s) 371, 1028
CviAII CATG 4 cut(s) 282, 537, 612, 666
CviQI GTAC 2 cut(s) 371, 1028
DdeI CTNAG 4 cut(s) 330, 344, 479, 584
DpnI GATC 3 cut(s) 237, 484, 1092
DpnII GATC 3 cut(s) 235, 482, 1090
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 2 cut(s) 143, 176
Eco130I CCWWGG 3 cut(s) 72, 231, 611
Eco147I AGGCCT 1 cut(s) 230
Eco32I GATATC 2 cut(s) 391, 773
Eco47I GGWCC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 319, 531
EcoRV GATATC 2 cut(s) 391, 773
EcoT14I CCWWGG 3 cut(s) 72, 231, 611
ErhI CCWWGG 3 cut(s) 72, 231, 611
FaeI CATG 4 cut(s) 285, 540, 615, 669
FatI CATG 4 cut(s) 281, 536, 611, 665
FauNDI CATATG 1 cut(s) 913
Fnu4HI GCNGC 5 cut(s) 98, 111, 132, 190, 306
FokI GGATG 7 cut(s) 20, 170, 308, 361, 509, 513, 839
Fsp4HI GCNGC 5 cut(s) 98, 111, 132, 190, 306
FspBI CTAG 1 cut(s) 381
GlaI GCGC 2 cut(s) 161, 163
GluI GCNGC 5 cut(s) 98, 111, 132, 190, 306
GsaI CCCAGC 1 cut(s) 205
HaeIII GGCC 7 cut(s) 71, 230, 278, 305, 566, 650, 815
HapII CCGG 1 cut(s) 39
HgaI GACGC 2 cut(s) 940, 1042
HhaI GCGC 2 cut(s) 162, 164
Hin1I GRCGYC 1 cut(s) 951
Hin1II CATG 4 cut(s) 285, 540, 615, 669
Hin6I GCGC 2 cut(s) 160, 162
HinP1I GCGC 2 cut(s) 160, 162
HinfI GANTC 3 cut(s) 176, 786, 871
HpaII CCGG 1 cut(s) 39
HphI GGTGA 3 cut(s) 100, 982, 1054
Hpy188I TCNGA 3 cut(s) 345, 920, 961
Hpy188III TCNNGA 3 cut(s) 126, 173, 934
Hpy99I CGWCG 2 cut(s) 143, 956
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 2 cut(s) 927, 1027
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 5 cut(s) 100, 271, 715, 742, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 195, 533
HpyF3I CTNAG 4 cut(s) 330, 344, 479, 584
HpySE526I ACGT 1 cut(s) 373
Hsp92I GRCGYC 1 cut(s) 951
Hsp92II CATG 4 cut(s) 285, 540, 615, 669
HspAI GCGC 2 cut(s) 160, 162
Kzo9I GATC 3 cut(s) 235, 482, 1090
LmnI GCTCC 2 cut(s) 94, 203
Lsp1109I GCAGC 2 cut(s) 84, 97
LweI GCATC 1 cut(s) 729
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 237, 484, 1092
MbiI CCGCTC 1 cut(s) 198
MboI GATC 3 cut(s) 235, 482, 1090
MboII GAAGA 7 cut(s) 18, 21, 24, 27, 272, 275, 308
MfeI CAATTG 1 cut(s) 863
MflI RGATCY 3 cut(s) 235, 482, 1090
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 3 cut(s) 688, 798, 863
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 780
MmeI TCCRAC 2 cut(s) 418, 898
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 4 cut(s) 405, 675, 998, 1056
MslI CAYNNNNRTG 2 cut(s) 284, 633
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 321, 533
MunI CAATTG 1 cut(s) 863
MvaI CCWGG 2 cut(s) 321, 533
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 68, 195, 533
NcoI CCATGG 1 cut(s) 611
NdeI CATATG 1 cut(s) 913
NdeII GATC 3 cut(s) 235, 482, 1090
NlaIII CATG 4 cut(s) 285, 540, 615, 669
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 44
NspI RCATGY 1 cut(s) 285
OliI CACNNNNGTG 2 cut(s) 284, 633
PaqCI CACCTGC 1 cut(s) 958
PauI GCGCGC 1 cut(s) 160
PceI AGGCCT 1 cut(s) 230
PciI ACATGT 1 cut(s) 281
PfeI GAWTC 2 cut(s) 176, 871
PkrI GCNGC 5 cut(s) 99, 112, 133, 191, 307
PleI GAGTC 1 cut(s) 780
PpsI GAGTC 1 cut(s) 780
PscI ACATGT 1 cut(s) 281
Psp6I CCWGG 2 cut(s) 319, 531
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 2 cut(s) 319, 531
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 4 cut(s) 276, 359, 565, 649
PstI CTGCAG 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 1093
PsuI RGATCY 3 cut(s) 235, 482, 1090
PteI GCGCGC 1 cut(s) 160
RsaI GTAC 2 cut(s) 372, 1029
RsaNI GTAC 2 cut(s) 371, 1028
RseI CAYNNNNRTG 2 cut(s) 284, 633
SaqAI TTAA 4 cut(s) 405, 675, 998, 1056
SatI GCNGC 5 cut(s) 98, 111, 132, 190, 306
Sau3AI GATC 3 cut(s) 235, 482, 1090
Sau96I GGNCC 4 cut(s) 276, 359, 565, 649
SchI GAGTC 1 cut(s) 780
ScrFI CCNGG 2 cut(s) 321, 533
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 2 cut(s) 98, 1094
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 2 cut(s) 284, 633
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 688, 798, 863
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 7 cut(s) 77, 132, 154, 187, 190, 196, 306
SspMI CTAG 1 cut(s) 381
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 319, 531
StyI CCWWGG 3 cut(s) 72, 231, 611
TaaI ACNGT 2 cut(s) 927, 1027
TaiI ACGT 1 cut(s) 376
TaqI TCGA 3 cut(s) 174, 796, 1049
TaqII GACCGA 1 cut(s) 347
TasI AATT 3 cut(s) 688, 798, 863
TauI GCSGC 3 cut(s) 134, 192, 308
TfiI GAWTC 2 cut(s) 176, 871
Tru1I TTAA 4 cut(s) 405, 675, 998, 1056
Tru9I TTAA 4 cut(s) 405, 675, 998, 1056
TscAI CASTG 2 cut(s) 49, 562
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 97, 110
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 300, 1082
TspRI CASTG 2 cut(s) 49, 562
VpaK11BI GGWCC 1 cut(s) 359
XceI RCATGY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 286
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.