MD06G1127700.v1.1

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006071
Physical Location & Seq
Forward (+)
26969672 .. 26974308
4637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1127700.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGGGGATGCGAAGAGGAACACTTCTTCTTCTTCCGGCGGTGACGAGGAGATACGAGCACAAGTGGCCAAGGCGGTGGAGGGGGTGAAGGAGCTGCAGGACTCAGCTGCCTCCTTCGTCTCCAGAGCCTCCGCCGACGAGCAATCCCTACGCCACCGCGCCCAATCTCTCGATTCCTCCATCCGACGCCTCCGCTCCCAGCTTGATTCCCTGCTCTCCAGCAAGGCCTTGGATCCCAAGTTAGCAGAAAAGCTGGAAGAAGACTTGCAGAGGGCCACATGTGTGTTGGTGGATGGCGATGCCGCTTCGTTTCTTCCTGGGAAATCTCAGGGGAGGTTTCTGAGGATGTTTCTCGGTCCTATCAACGTGCGTTCCTCTAGCAAAGATATCCGACTCAAGGTTAAAGAGGAATACAACGGTTACAGAGATAGAACTGCCCTTCTATTCCTTCTTTTTCCAGCAGCACTACTAACACTAAGATCTTGGATTTGGGATGGATGTTTGCCAGCGTTTCCAGTTCAGGCTTACCAGGCATGGTTGTTATTCCTCTACACTGGTTTGGCACTGCGAGAAAACATTTTGAGAGTAAATGGAAGCGATATTCGTCCATGGTGGATATACCATCACTATTGTGCTATGTTTATGGCCCTTGTTAGTCTCACATGGGAGATTAAAGGACAACCAAATTGTTCCCAAAAGCAGAGAGGTGTGCAATTTTTCCTACAATGGGCTATGATGCAAGGAGTTGCTATGCTTTTACAAAATAGATATCAACGCCAGAGACTCTATACTCGAATTGCATTGGGAAAGGCCAAAAGAATGGATGTGGTATGGGGAGAAACAGCAGGTGTAGATGGTCAATTATGGATTCTATGTCCAATACTATTTATTTTACAGGGTTTTGAGGCATATGTCGGAATACAGTTGCTTACGACTGCTTTTATTGGCATTGCTTCGGAATGGCAGGTGAGTTTTTGCGGCGTACTGTTAATTTTAATGGCAGTTGTGAACTTTGTAAATACGGTAGAGACGCTTATGGTGAAGTCAAGGTTTAAGGCAAAGATGAAAAGAAGTAAGAGCAAGCAGGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

365

Amino Acids

41.19

Weight (kDa)

9.59

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 22 - 351 7.3e-97 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 836, 955
Acc36I ACCTGC 2 cut(s) 836, 955
AccBSI CCGCTC 1 cut(s) 195
AccII CGCG 1 cut(s) 159
AciI CCGC 7 cut(s) 39, 74, 132, 157, 193, 303, 978
AclWI GGATC 2 cut(s) 227, 240
AcoI YGGCCR 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 984
AfiI CCNNNNNNNGG 2 cut(s) 397, 727
AflIII ACRYGT 1 cut(s) 278
AjnI CCWGG 2 cut(s) 316, 528
AleI CACNNNNGTG 2 cut(s) 281, 630
AluBI AGCT 4 cut(s) 94, 107, 202, 253
AluI AGCT 4 cut(s) 94, 107, 202, 253
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 4 cut(s) 124, 662, 775, 1022
AlwI GGATC 2 cut(s) 227, 240
AoxI GGCC 5 cut(s) 66, 225, 273, 645, 810
ApeKI GCWGC 3 cut(s) 94, 107, 461
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 3 cut(s) 273, 356, 646
AsuHPI GGTGA 4 cut(s) 53, 97, 979, 1051
AvaII GGWCC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 3 cut(s) 81, 94, 473
BccI CCATC 5 cut(s) 188, 287, 488, 630, 848
BciT130I CCWGG 2 cut(s) 318, 530
BcoDI GTCTC 4 cut(s) 124, 662, 775, 1022
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 95
BfuAI ACCTGC 2 cut(s) 836, 955
BglII AGATCT 1 cut(s) 479
BisI GCNGC 5 cut(s) 95, 108, 303, 462, 979
BlsI GCNGC 5 cut(s) 96, 109, 304, 463, 980
Bme1390I CCNGG 2 cut(s) 318, 530
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 3 cut(s) 273, 356, 646
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 318, 530
BmsI GCATC 2 cut(s) 289, 726
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 2 cut(s) 106, 202
BpuEI CTTGAG 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 187
BsaJI CCNNGG 4 cut(s) 69, 228, 317, 608
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 727
Bse1I ACTGG 2 cut(s) 515, 559
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 2 cut(s) 318, 530
BseDI CCNNGG 4 cut(s) 69, 228, 317, 608
BseGI GGATG 7 cut(s) 13, 180, 298, 351, 499, 503, 829
BseLI CCNNNNNNNGG 2 cut(s) 397, 727
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 3 cut(s) 117, 332, 341
BseNI ACTGG 2 cut(s) 515, 559
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 3 cut(s) 81, 94, 473
BseYI CCCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 5 cut(s) 68, 227, 275, 647, 812
BsiHKAI GWGCWC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 397, 727
BsmAI GTCTC 4 cut(s) 124, 662, 775, 1022
BsmBI CGTCTC 2 cut(s) 124, 1022
BsnI GGCC 5 cut(s) 68, 227, 275, 647, 812
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 2 cut(s) 232, 479
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 7 cut(s) 39, 74, 132, 157, 193, 303, 978
BspANI GGCC 5 cut(s) 68, 227, 275, 647, 812
BspCNI CTCAG 3 cut(s) 116, 333, 340
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 234
BspMAI CTGCAG 1 cut(s) 99
BspMI ACCTGC 2 cut(s) 836, 955
BspPI GGATC 2 cut(s) 227, 240
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 2 cut(s) 515, 559
BssECI CCNNGG 4 cut(s) 69, 228, 317, 608
BssMI GATC 2 cut(s) 232, 479
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 3 cut(s) 69, 228, 608
Bst2UI CCWGG 2 cut(s) 318, 530
Bst4CI ACNGT 4 cut(s) 419, 924, 987, 1024
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 1 cut(s) 187
BstC8I GCNNGC 2 cut(s) 507, 1082
BstDEI CTNAG 4 cut(s) 103, 327, 341, 476
BstDSI CCRYGG 1 cut(s) 608
BstF5I GGATG 7 cut(s) 13, 180, 298, 351, 499, 503, 829
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 235, 482
BstMAI GTCTC 4 cut(s) 124, 662, 775, 1022
BstMBI GATC 2 cut(s) 232, 479
BstMWI GCNNNNNNNGC 2 cut(s) 65, 530
BstNI CCWGG 2 cut(s) 318, 530
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 316, 528
BstSFI CTRYAG 1 cut(s) 95
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 3 cut(s) 81, 94, 473
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 2 cut(s) 232, 479
BstXI CCANNNNNNTGG 2 cut(s) 76, 820
BstYI RGATCY 2 cut(s) 232, 479
BsuRI GGCC 5 cut(s) 68, 227, 275, 647, 812
BtgI CCRYGG 1 cut(s) 608
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 7 cut(s) 13, 180, 298, 351, 499, 503, 829
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 2 cut(s) 552, 563
BveI ACCTGC 2 cut(s) 836, 955
Cac8I GCNNGC 2 cut(s) 507, 1082
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 273, 356, 646
CseI GACGC 2 cut(s) 195, 1039
Csp6I GTAC 1 cut(s) 983
CviAII CATG 4 cut(s) 279, 534, 609, 663
CviQI GTAC 1 cut(s) 983
DdeI CTNAG 4 cut(s) 103, 327, 341, 476
DpnI GATC 2 cut(s) 234, 481
DpnII GATC 2 cut(s) 232, 479
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 69, 228, 608
Eco147I AGGCCT 1 cut(s) 227
Eco32I GATATC 2 cut(s) 388, 770
Eco47I GGWCC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 316, 528
EcoRV GATATC 2 cut(s) 388, 770
EcoT14I CCWWGG 3 cut(s) 69, 228, 608
ErhI CCWWGG 3 cut(s) 69, 228, 608
Esp3I CGTCTC 2 cut(s) 124, 1022
FaeI CATG 4 cut(s) 282, 537, 612, 666
FatI CATG 4 cut(s) 278, 533, 608, 662
FauNDI CATATG 1 cut(s) 910
Fnu4HI GCNGC 5 cut(s) 95, 108, 303, 462, 979
FokI GGATG 7 cut(s) 20, 167, 305, 358, 506, 510, 836
Fsp4HI GCNGC 5 cut(s) 95, 108, 303, 462, 979
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 160
GluI GCNGC 5 cut(s) 95, 108, 303, 462, 979
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 2 cut(s) 106, 202
HaeIII GGCC 5 cut(s) 68, 227, 275, 647, 812
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 195, 1039
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 4 cut(s) 282, 537, 612, 666
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 6 cut(s) 101, 173, 206, 393, 783, 868
HpaII CCGG 1 cut(s) 36
HphI GGTGA 4 cut(s) 53, 97, 979, 1051
Hpy166II GTNNAC 1 cut(s) 1009
Hpy188I TCNGA 5 cut(s) 185, 342, 392, 917, 958
Hpy188III TCNNGA 2 cut(s) 123, 170
Hpy8I GTNNAC 1 cut(s) 1009
Hpy99I CGWCG 2 cut(s) 140, 189
HpyAV CCTTC 4 cut(s) 82, 124, 449, 458
HpyCH4III ACNGT 4 cut(s) 419, 924, 987, 1024
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 5 cut(s) 97, 268, 712, 739, 800
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 530
HpyF3I CTNAG 4 cut(s) 103, 327, 341, 476
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 4 cut(s) 282, 537, 612, 666
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 232, 479
LmnI GCTCC 2 cut(s) 91, 200
Lsp1109I GCAGC 3 cut(s) 81, 94, 473
LweI GCATC 2 cut(s) 289, 726
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 41, 419
MalI GATC 2 cut(s) 234, 481
MbiI CCGCTC 1 cut(s) 195
MboI GATC 2 cut(s) 232, 479
MboII GAAGA 7 cut(s) 18, 21, 24, 25, 269, 272, 305
MflI RGATCY 2 cut(s) 232, 479
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 5 cut(s) 685, 713, 795, 860, 990
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 3 cut(s) 95, 387, 777
MmeI TCCRAC 3 cut(s) 208, 415, 895
Mox20I TGGCCA 1 cut(s) 68
MscI TGGCCA 1 cut(s) 68
MseI TTAA 5 cut(s) 402, 672, 989, 995, 1053
MslI CAYNNNNRTG 2 cut(s) 281, 630
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 318, 530
MvaI CCWGG 2 cut(s) 318, 530
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 65, 530
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 1 cut(s) 910
NdeII GATC 2 cut(s) 232, 479
NlaIII CATG 4 cut(s) 282, 537, 612, 666
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 41
NspI RCATGY 1 cut(s) 282
OliI CACNNNNGTG 2 cut(s) 281, 630
PaqCI CACCTGC 2 cut(s) 836, 955
PceI AGGCCT 1 cut(s) 227
PciI ACATGT 1 cut(s) 278
PfeI GAWTC 3 cut(s) 173, 206, 868
PkrI GCNGC 5 cut(s) 96, 109, 304, 463, 980
PleI GAGTC 3 cut(s) 95, 387, 777
PpsI GAGTC 3 cut(s) 95, 387, 777
PscI ACATGT 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 316, 528
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 2 cut(s) 316, 528
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 3 cut(s) 273, 356, 646
PstI CTGCAG 1 cut(s) 99
PsuI RGATCY 2 cut(s) 232, 479
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 1 cut(s) 984
RsaNI GTAC 1 cut(s) 983
RseI CAYNNNNRTG 2 cut(s) 281, 630
SaqAI TTAA 5 cut(s) 402, 672, 989, 995, 1053
SatI GCNGC 5 cut(s) 95, 108, 303, 462, 979
Sau3AI GATC 2 cut(s) 232, 479
Sau96I GGNCC 3 cut(s) 273, 356, 646
SchI GAGTC 3 cut(s) 95, 387, 777
ScrFI CCNGG 2 cut(s) 318, 530
SduI GDGCHC 1 cut(s) 61
SfaNI GCATC 2 cut(s) 289, 726
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 2 cut(s) 281, 630
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 5 cut(s) 685, 713, 795, 860, 990
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 7 cut(s) 39, 74, 132, 157, 193, 303, 978
SspMI CTAG 1 cut(s) 378
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 316, 528
StyI CCWWGG 3 cut(s) 69, 228, 608
TaaI ACNGT 4 cut(s) 419, 924, 987, 1024
TaiI ACGT 1 cut(s) 369
TaqI TCGA 2 cut(s) 171, 793
TaqII GACCGA 1 cut(s) 344
TasI AATT 5 cut(s) 685, 713, 795, 860, 990
TauI GCSGC 2 cut(s) 305, 981
TfiI GAWTC 3 cut(s) 173, 206, 868
Tru1I TTAA 5 cut(s) 402, 672, 989, 995, 1053
Tru9I TTAA 5 cut(s) 402, 672, 989, 995, 1053
TscAI CASTG 2 cut(s) 559, 570
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 3 cut(s) 94, 107, 461
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 1079
TspRI CASTG 2 cut(s) 559, 570
VpaK11BI GGWCC 1 cut(s) 356
XceI RCATGY 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 2 cut(s) 226, 283
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.