RLG00000004724

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006071
Physical Location & Seq
Reverse (-)
61738289 .. 61742437
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004724

Sequence Viewer

Length: 1104 bp
ATGGGAGATGATGCGAAGAAGAAGAGCTCTTGTTCTGATTCGCCTACTCTGGACGCCCTACAAGCTGAAGTCACCACAGCCATGGAGGGCGCCAAGTCGTTACAGGACTCAGCTTCCTCCTTCATCTCCAGAAACTCCAGCGAAGAACAGTCGCTTCGCCAGCGAGCCCAGTCTCTCGATTCCACAATCCGCCGCCTCCGTTCCCAAATCTATTCTCTTCATCACGACAACAAGGCCGTCGATCCCAAGCTCTGCGAAAAGCTGGAAGAAGATTTGCACAGGGCCACGTGTGTTTTGACGGACGGCGACGCCGGTTCTTTTCTTCCTCCCAAAGCTCACGGGGGGTTTCTGAGGATGTTTCTTGGTCCTATCAATGTGCGTTCGTCTAGCAAAGACATCCAACTCAAGGTTAAAGAGGAATACAATGCTTACAGAGATCGAACTGCCCTGCTATTCCTTCTCTTTCCATTTGCACTTCTTATTCTGAGATCTTGGATATGGGATGGATGCTTACCAACATTTCCTGTTCAAGCTTACCAGGCATGGCTCTTATTCCTCTACACTGGTTTGTCATTGCGAGAAAACATACTGAGAGTAAACGGAAGTGATATTCGTCCATGGTGGATATACCATCACTATTGTGCTATGTTTATGGCCATGGTTAGTCTCACATGGGATATTCAAGGACAACCAAATTGTGCCCAAAAGCAGAGAGGGGTGGAACTTTTCCTGCAATGGGCAATGATGCAAGGAGTGGCTATGCTTTTACAAAATAGATATCAACGCCAGAGACTGTATACTCGAATTGCATTGGGAAAGGCCAATAGGATGGACGTCGTATGGGGTGAAACAGCTGGTGTTGATGGTCAATTGTGGCTTCTATGTCCCATACTTTTTATAATGCAGGGTTTTGAGGCATATGTTGGACTACAGTTGCTTAAGACTGCTTTGTTCGGGGATGTTACCGAATGGCAGGTGAGTTTGTGCGGTGTGCTATTAATTCTTATGGCGATCGGGAACTTTGTAAATACTGTACAAACACTCATGGTGAAATCGAGGTTCAAGGCAAAGATGAAAAAGACCAAGAGCAAGCAGGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

368

Amino Acids

41.77

Weight (kDa)

9.11

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 25 - 354 2.6e-99 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 1 cut(s) 964
AatII GACGTC 1 cut(s) 837
Acc36I ACCTGC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 797
AciI CCGC 3 cut(s) 190, 193, 987
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 654
AcuI CTGAAG 1 cut(s) 87
AcvI CACGTG 1 cut(s) 288
AcyI GRCGYC 4 cut(s) 54, 90, 309, 834
AfaI GTAC 1 cut(s) 1035
AfiI CCNNNNNNNGG 2 cut(s) 406, 736
AflII CTTAAG 1 cut(s) 938
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 3 cut(s) 530, 683, 1063
AjnI CCWGG 1 cut(s) 537
AleI CACNNNNGTG 1 cut(s) 639
AluBI AGCT 8 cut(s) 27, 65, 113, 250, 262, 335, 533, 854
AluI AGCT 8 cut(s) 27, 65, 113, 250, 262, 335, 533, 854
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 3 cut(s) 177, 671, 784
AlwI GGATC 1 cut(s) 236
AlwNI CAGNNNCTG 1 cut(s) 793
AoxI GGCC 4 cut(s) 234, 282, 654, 819
AseI ATTAAT 1 cut(s) 998
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 282, 365
AsuHPI GGTGA 4 cut(s) 64, 857, 988, 1060
AvaII GGWCC 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 703
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 29, 169
BbrPI CACGTG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 29
BccI CCATC 4 cut(s) 497, 639, 823, 857
BceAI ACGGC 2 cut(s) 221, 319
BciT130I CCWGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 177, 671, 784
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 929
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 964
BglII AGATCT 1 cut(s) 488
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 282, 365
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 497, 735
BmuI ACTGGG 1 cut(s) 163
BpmI CTGGAG 2 cut(s) 112, 121
BpuEI CTTGAG 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 288
BsaHI GRCGYC 4 cut(s) 54, 90, 309, 834
BsaJI CCNNGG 3 cut(s) 81, 617, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 736
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 169, 568
Bse3DI GCAATG 3 cut(s) 572, 740, 747
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 3 cut(s) 81, 617, 657
BseGI GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BseLI CCNNNNNNNGG 2 cut(s) 406, 736
BseMI GCAATG 3 cut(s) 572, 740, 747
BseMII CTCAG 4 cut(s) 123, 341, 476, 581
BseNI ACTGG 2 cut(s) 169, 568
BseSI GKGCMC 1 cut(s) 703
Bsh1285I CGRYCG 1 cut(s) 1014
BshFI GGCC 4 cut(s) 236, 284, 656, 821
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 2 cut(s) 406, 736
BsmAI GTCTC 3 cut(s) 177, 671, 784
BsmFI GGGAC 1 cut(s) 870
BsnI GGCC 4 cut(s) 236, 284, 656, 821
Bsp1286I GDGCHC 3 cut(s) 29, 169, 703
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 4 cut(s) 241, 436, 488, 1011
Bsp19I CCATGG 3 cut(s) 81, 617, 657
BspACI CCGC 3 cut(s) 190, 193, 987
BspANI GGCC 4 cut(s) 236, 284, 656, 821
BspCNI CTCAG 4 cut(s) 122, 342, 477, 582
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 938
BsrDI GCAATG 3 cut(s) 572, 740, 747
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 2 cut(s) 169, 568
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 3 cut(s) 81, 617, 657
BssMI GATC 4 cut(s) 241, 436, 488, 1011
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 4 cut(s) 54, 90, 309, 834
BssT1I CCWWGG 3 cut(s) 81, 617, 657
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 4 cut(s) 150, 795, 933, 1033
Bst6I CTCTTC 2 cut(s) 17, 222
BstACI GRCGYC 4 cut(s) 54, 90, 309, 834
BstAFI CTTAAG 1 cut(s) 938
BstAUI TGTACA 1 cut(s) 1033
BstBAI YACGTR 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 161, 165, 1091
BstDEI CTNAG 4 cut(s) 109, 350, 485, 590
BstDSI CCRYGG 3 cut(s) 81, 617, 657
BstF5I GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 4 cut(s) 244, 439, 491, 1014
BstMAI GTCTC 3 cut(s) 177, 671, 784
BstMBI GATC 4 cut(s) 241, 436, 488, 1011
BstMCI CGRYCG 1 cut(s) 1014
BstMWI GCNNNNNNNGC 3 cut(s) 62, 160, 539
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 929
BstSLI GKGCMC 1 cut(s) 703
BstX2I RGATCY 1 cut(s) 488
BstXI CCANNNNNNTGG 2 cut(s) 82, 829
BstYI RGATCY 1 cut(s) 488
BstZ17I GTATAC 1 cut(s) 798
BsuRI GGCC 4 cut(s) 236, 284, 656, 821
BtgI CCRYGG 3 cut(s) 81, 617, 657
BtsCI GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BtsIMutI CAGTG 1 cut(s) 561
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 3 cut(s) 161, 165, 1091
CaiI CAGNNNCTG 1 cut(s) 793
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 282, 365
CseI GACGC 2 cut(s) 62, 317
Csp6I GTAC 1 cut(s) 1034
CviAII CATG 6 cut(s) 82, 543, 618, 658, 672, 1045
CviQI GTAC 1 cut(s) 1034
DdeI CTNAG 4 cut(s) 109, 350, 485, 590
DinI GGCGCC 1 cut(s) 91
DpnI GATC 4 cut(s) 243, 438, 490, 1013
DpnII GATC 4 cut(s) 241, 436, 488, 1011
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 2 cut(s) 17, 222
EarI CTCTTC 2 cut(s) 17, 222
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 81, 617, 657
Eco24I GRGCYC 2 cut(s) 29, 169
Eco32I GATATC 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 87
Eco72I CACGTG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 779
EcoT14I CCWWGG 3 cut(s) 81, 617, 657
EcoT38I GRGCYC 2 cut(s) 29, 169
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 81, 617, 657
FaeI CATG 6 cut(s) 85, 546, 621, 661, 675, 1048
FaqI GGGAC 1 cut(s) 870
FatI CATG 6 cut(s) 81, 542, 617, 657, 671, 1044
FauNDI CATATG 1 cut(s) 919
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 6 cut(s) 367, 383, 515, 519, 841, 971
FriOI GRGCYC 2 cut(s) 29, 169
Fsp4HI GCNGC 1 cut(s) 193
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 91
GluI GCNGC 1 cut(s) 193
GsuI CTGGAG 2 cut(s) 112, 121
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 4 cut(s) 236, 284, 656, 821
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 62, 317
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 4 cut(s) 54, 90, 309, 834
Hin1II CATG 6 cut(s) 85, 546, 621, 661, 675, 1048
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 3 cut(s) 38, 107, 179
HpaII CCGG 1 cut(s) 312
HphI GGTGA 4 cut(s) 64, 857, 988, 1060
Hpy166II GTNNAC 2 cut(s) 598, 798
Hpy188I TCNGA 3 cut(s) 37, 351, 486
Hpy188III TCNNGA 5 cut(s) 50, 129, 176, 224, 1015
Hpy8I GTNNAC 2 cut(s) 598, 798
Hpy99I CGWCG 3 cut(s) 242, 311, 839
HpyAV CCTTC 2 cut(s) 130, 467
HpyCH4III ACNGT 4 cut(s) 150, 795, 933, 1033
HpyCH4IV ACGT 2 cut(s) 287, 834
HpyCH4V TGCA 6 cut(s) 277, 473, 733, 748, 809, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 160, 539
HpyF3I CTNAG 4 cut(s) 109, 350, 485, 590
HpySE526I ACGT 2 cut(s) 287, 834
Hsp92I GRCGYC 4 cut(s) 54, 90, 309, 834
Hsp92II CATG 6 cut(s) 85, 546, 621, 661, 675, 1048
HspAI GCGC 1 cut(s) 90
KasI GGCGCC 1 cut(s) 89
Kzo9I GATC 4 cut(s) 241, 436, 488, 1011
LguI GCTCTTC 1 cut(s) 17
LweI GCATC 2 cut(s) 497, 735
MaeI CTAG 1 cut(s) 387
MaeII ACGT 2 cut(s) 287, 834
MaeIII GTNAC 3 cut(s) 70, 99, 961
MalI GATC 4 cut(s) 243, 438, 490, 1013
MboI GATC 4 cut(s) 241, 436, 488, 1011
MboII GAAGA 8 cut(s) 28, 31, 34, 155, 209, 278, 281, 314
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 1 cut(s) 488
MhlI GDGCHC 3 cut(s) 29, 169, 703
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 5 cut(s) 694, 804, 869, 999, 1097
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 424, 904
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 411, 939, 998
MslI CAYNNNNRTG 2 cut(s) 80, 639
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 1 cut(s) 854
MspCI CTTAAG 1 cut(s) 938
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 539
MunI CAATTG 1 cut(s) 869
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 62, 160, 539
NarI GGCGCC 1 cut(s) 90
NcoI CCATGG 3 cut(s) 81, 617, 657
NdeI CATATG 1 cut(s) 919
NdeII GATC 4 cut(s) 241, 436, 488, 1011
NlaIII CATG 6 cut(s) 85, 546, 621, 661, 675, 1048
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 639
PaqCI CACCTGC 1 cut(s) 964
PciSI GCTCTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 38, 179
PkrI GCNGC 1 cut(s) 194
Ple19I CGATCG 1 cut(s) 1014
PleI GAGTC 1 cut(s) 101
PluTI GGCGCC 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 288
PmlI CACGTG 1 cut(s) 288
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 998
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 537
PspCI CACGTG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 282, 365
PstNI CAGNNNCTG 1 cut(s) 793
PsuI RGATCY 1 cut(s) 488
PvuI CGATCG 1 cut(s) 1014
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 1 cut(s) 1035
RsaNI GTAC 1 cut(s) 1034
RseI CAYNNNNRTG 2 cut(s) 80, 639
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 3 cut(s) 411, 939, 998
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 4 cut(s) 241, 436, 488, 1011
Sau96I GGNCC 2 cut(s) 282, 365
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 3 cut(s) 29, 169, 703
SfaNI GCATC 2 cut(s) 497, 735
SfcI CTRYAG 1 cut(s) 929
SfoI GGCGCC 1 cut(s) 91
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 80, 639
SmlI CTYRAG 2 cut(s) 404, 938
SmoI CTYRAG 2 cut(s) 404, 938
Sse9I AATT 5 cut(s) 694, 804, 869, 999, 1097
SsiI CCGC 3 cut(s) 190, 193, 987
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 3 cut(s) 81, 617, 657
TaaI ACNGT 4 cut(s) 150, 795, 933, 1033
TaiI ACGT 2 cut(s) 290, 837
TaqI TCGA 5 cut(s) 177, 240, 439, 802, 1055
TasI AATT 5 cut(s) 694, 804, 869, 999, 1097
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 38, 179
Tru1I TTAA 3 cut(s) 411, 939, 998
Tru9I TTAA 3 cut(s) 411, 939, 998
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 112, 209, 1088
TspGWI ACGGA 3 cut(s) 188, 314, 615
TspRI CASTG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 365
VspI ATTAAT 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 1 cut(s) 387
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.