FvH4_5g10150

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006071
Physical Location & Seq
Reverse (-)
5789929 .. 5794683
4755 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10150.t1

Sequence Viewer

Length: 1092 bp
ATGGGAGATGATGCTGCGAACAAGAACTCCTCTGTTTTGGAGACCCTTCAAGCTGAAGTAACCAAAGCTATGGAAGGCGCCAAGTCTTTACAGGAGTCAGCTTCCTCCTTCATCTCCCGAAACTCCAGCGAAGAACAGTCGCTCCGCCACCGCGCCCAGTCTCTCGATTCCACCATCCGCCGCCTCCGCTCCCAAATCTACTCTCTCCACCATGACAACAAGGCCTTGGATCCCAAGCTCGCCGACAAGCTGGAAGAGGATTTGCATAGGGCGACGTGTATTTTGACGGACGGTGACGCCGGTTCTTTTCTGCCTCCGAAAGCTCATGGTGGGTTTTTGAGGATGTTTCTTGGTCCTATCAATGTGCGTTCGTCTAGCAAGGACATCCAGCTCAAGGTTAAAGAGGAATACAATGCTTATAGAGATCGAACTGCCCTGCTATTCCTTCTCTTTCCATTAGCACTTCTTATTCTGAGATCTTGGGTATGGGATGGATGCTTGCCAACATTTCCTGTTCAGGCTTACCAGGCATGGCTCTTATTTCTCTACACTGGTCTGTCATTGCGAGAAAACATACTGAGAGTAAACGGAAGTGATATTCGTCCATGGTGGATATACCATCATTATTGCGCTATGTTTATGGCCATGGTTAGTCTCACATGGGATATTCAAGGACAACCGAATTGTGCCCAAAAGCAGAGAGGGGTGCAACTTTTCCTGCAATGGGCAATGATGCAAGGAGTGGCTATGCTTTTACAAAATAGATATCAACGCCAGAGACTATATACTCGAATTGCATTGGGAAAGGCCAATAGGATGGACGTTGTATGGGGTGAAACAGCTGGTGTTGATGGTCAACTGTGGCTTCTATGTCCCATACTCTTTATAATGCAGGGTTTTGAGGCATATGTTGGACTACAGTTGCTCAAGACTGCTTTGTTCGGGGTTGTTACCGAATGGCAGGTGAGTTTGTGCGGTGTGCTATTAATTTTAATGGCGATCGGGAACTTTGTAAATACTGTACAAACACTAATGGTAAAATCAAGGTTCAAGGCAAAGATGAAAAAAACCAAGAGCAAGCAGGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

364

Amino Acids

41.33

Weight (kDa)

9.37

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 13 - 350 6.4e-99 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
AarI CACCTGC 1 cut(s) 952
Acc36I ACCTGC 1 cut(s) 952
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 189
AccII CGCG 1 cut(s) 153
AciI CCGC 6 cut(s) 145, 151, 178, 181, 187, 975
AclWI GGATC 2 cut(s) 224, 237
AcoI YGGCCR 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 2 cut(s) 78, 297
AfaI GTAC 1 cut(s) 1023
AfiI CCNNNNNNNGG 2 cut(s) 394, 724
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 3 cut(s) 50, 671, 1051
AjiI CACGTC 1 cut(s) 276
AjnI CCWGG 1 cut(s) 525
AloI GAACNNNNNNTCC 4 cut(s) 11, 43, 126, 158
AluBI AGCT 8 cut(s) 53, 68, 101, 238, 250, 323, 391, 842
AluI AGCT 8 cut(s) 53, 68, 101, 238, 250, 323, 391, 842
Alw26I GTCTC 4 cut(s) 35, 165, 659, 772
AlwI GGATC 2 cut(s) 224, 237
AoxI GGCC 3 cut(s) 222, 642, 807
ApeKI GCWGC 1 cut(s) 14
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
AseI ATTAAT 1 cut(s) 986
AspLEI GCGC 3 cut(s) 80, 155, 632
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 305, 845, 976
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 691
BalI TGGCCA 1 cut(s) 644
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 77
BccI CCATC 5 cut(s) 182, 485, 627, 811, 845
BciT130I CCWGG 1 cut(s) 527
BcoDI GTCTC 4 cut(s) 35, 165, 659, 772
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 917
BfoI RGCGCY 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 952
BglII AGATCT 1 cut(s) 476
BisI GCNGC 2 cut(s) 15, 181
BlsI GCNGC 2 cut(s) 16, 182
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 79, 231
BmrFI CCNGG 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 485, 723
BmuI ACTGGG 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 109
BpuEI CTTGAG 2 cut(s) 377, 911
BsaHI GRCGYC 2 cut(s) 78, 297
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 225, 605, 645
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 126, 156
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 724
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 157, 556
Bse3DI GCAATG 3 cut(s) 560, 728, 735
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 3 cut(s) 225, 605, 645
BseGI GGATG 6 cut(s) 174, 348, 384, 496, 500, 822
BseLI CCNNNNNNNGG 2 cut(s) 394, 724
BseMI GCAATG 3 cut(s) 560, 728, 735
BseMII CTCAG 2 cut(s) 464, 569
BseNI ACTGG 2 cut(s) 157, 556
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 691
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 1002
BshFI GGCC 3 cut(s) 224, 644, 809
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 1002
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 2 cut(s) 394, 724
BsmAI GTCTC 4 cut(s) 35, 165, 659, 772
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 3 cut(s) 224, 644, 809
Bso31I GGTCTC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 691
Bsp1407I TGTACA 1 cut(s) 1021
Bsp143I GATC 4 cut(s) 229, 424, 476, 999
Bsp19I CCATGG 2 cut(s) 605, 645
BspACI CCGC 6 cut(s) 145, 151, 178, 181, 187, 975
BspANI GGCC 3 cut(s) 224, 644, 809
BspCNI CTCAG 2 cut(s) 465, 570
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 79, 231
BspMI ACCTGC 1 cut(s) 952
BspPI GGATC 2 cut(s) 224, 237
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 189
BsrDI GCAATG 3 cut(s) 560, 728, 735
BsrFI RCCGGY 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 1021
BsrI ACTGG 2 cut(s) 157, 556
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 225, 605, 645
BssMI GATC 4 cut(s) 229, 424, 476, 999
BssNI GRCGYC 2 cut(s) 78, 297
BssT1I CCWWGG 3 cut(s) 225, 605, 645
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 5 cut(s) 138, 293, 861, 921, 1021
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 2 cut(s) 78, 297
BstAUI TGTACA 1 cut(s) 1021
BstC8I GCNNGC 3 cut(s) 240, 500, 1079
BstDEI CTNAG 2 cut(s) 473, 578
BstDSI CCRYGG 2 cut(s) 605, 645
BstF5I GGATG 6 cut(s) 174, 348, 384, 496, 500, 822
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 3 cut(s) 80, 155, 632
BstKTI GATC 4 cut(s) 232, 427, 479, 1002
BstMAI GTCTC 4 cut(s) 35, 165, 659, 772
BstMBI GATC 4 cut(s) 229, 424, 476, 999
BstMCI CGRYCG 1 cut(s) 1002
BstMWI GCNNNNNNNGC 2 cut(s) 186, 527
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 917
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 229, 476
BstXI CCANNNNNNTGG 2 cut(s) 70, 817
BstYI RGATCY 2 cut(s) 229, 476
BsuRI GGCC 3 cut(s) 224, 644, 809
BtgI CCRYGG 2 cut(s) 605, 645
BtrI CACGTC 1 cut(s) 276
BtsCI GGATG 6 cut(s) 174, 348, 384, 496, 500, 822
BtsIMutI CAGTG 1 cut(s) 549
BveI ACCTGC 1 cut(s) 952
Cac8I GCNNGC 3 cut(s) 240, 500, 1079
CfoI GCGC 3 cut(s) 80, 155, 632
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 1022
CviAII CATG 6 cut(s) 212, 326, 531, 606, 646, 660
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 2 cut(s) 473, 578
DinI GGCGCC 1 cut(s) 79
DpnI GATC 4 cut(s) 231, 426, 478, 1001
DpnII GATC 4 cut(s) 229, 424, 476, 999
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
EciI GGCGGA 2 cut(s) 134, 167
Eco130I CCWWGG 3 cut(s) 225, 605, 645
Eco147I AGGCCT 1 cut(s) 224
Eco31I GGTCTC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 525
EcoRV GATATC 1 cut(s) 767
EcoT14I CCWWGG 3 cut(s) 225, 605, 645
EgeI GGCGCC 1 cut(s) 79
EheI GGCGCC 1 cut(s) 79
ErhI CCWWGG 3 cut(s) 225, 605, 645
FaeI CATG 6 cut(s) 215, 329, 534, 609, 649, 663
FaqI GGGAC 1 cut(s) 858
FatI CATG 6 cut(s) 211, 325, 530, 605, 645, 659
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 15, 181
FokI GGATG 6 cut(s) 161, 355, 371, 503, 507, 829
Fsp4HI GCNGC 2 cut(s) 15, 181
FspBI CTAG 1 cut(s) 375
GlaI GCGC 3 cut(s) 79, 154, 631
GluI GCNGC 2 cut(s) 15, 181
GsuI CTGGAG 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 3 cut(s) 224, 644, 809
HapII CCGG 1 cut(s) 300
HgaI GACGC 1 cut(s) 305
HhaI GCGC 3 cut(s) 80, 155, 632
Hin1I GRCGYC 2 cut(s) 78, 297
Hin1II CATG 6 cut(s) 215, 329, 534, 609, 649, 663
Hin6I GCGC 3 cut(s) 78, 153, 630
HinP1I GCGC 3 cut(s) 78, 153, 630
HincII GTYRAC 1 cut(s) 857
HindII GTYRAC 1 cut(s) 857
HinfI GANTC 2 cut(s) 95, 167
HpaII CCGG 1 cut(s) 300
HphI GGTGA 3 cut(s) 305, 845, 976
Hpy166II GTNNAC 2 cut(s) 586, 857
Hpy188I TCNGA 2 cut(s) 318, 474
Hpy188III TCNNGA 4 cut(s) 117, 164, 928, 1003
Hpy8I GTNNAC 2 cut(s) 586, 857
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 4 cut(s) 56, 68, 118, 455
HpyCH4III ACNGT 5 cut(s) 138, 293, 861, 921, 1021
HpyCH4IV ACGT 2 cut(s) 275, 822
HpyCH4V TGCA 6 cut(s) 265, 709, 721, 736, 797, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 527
HpyF3I CTNAG 2 cut(s) 473, 578
HpySE526I ACGT 2 cut(s) 275, 822
Hsp92I GRCGYC 2 cut(s) 78, 297
Hsp92II CATG 6 cut(s) 215, 329, 534, 609, 649, 663
HspAI GCGC 3 cut(s) 78, 153, 630
KasI GGCGCC 1 cut(s) 77
Kzo9I GATC 4 cut(s) 229, 424, 476, 999
LmnI GCTCC 2 cut(s) 147, 194
LweI GCATC 2 cut(s) 485, 723
MaeI CTAG 1 cut(s) 375
MaeII ACGT 2 cut(s) 275, 822
MaeIII GTNAC 3 cut(s) 58, 293, 949
MalI GATC 4 cut(s) 231, 426, 478, 1001
MbiI CCGCTC 1 cut(s) 189
MboI GATC 4 cut(s) 229, 424, 476, 999
MboII GAAGA 2 cut(s) 143, 266
MflI RGATCY 2 cut(s) 229, 476
MhlI GDGCHC 1 cut(s) 691
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 4 cut(s) 682, 792, 987, 1085
MluNI TGGCCA 1 cut(s) 644
Mly113I GGCGCC 1 cut(s) 78
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 9 cut(s) 40, 115, 194, 250, 324, 333, 397, 695, 895
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 3 cut(s) 399, 986, 992
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 1 cut(s) 527
MvaI CCWGG 1 cut(s) 527
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 186, 527
NarI GGCGCC 1 cut(s) 78
NcoI CCATGG 2 cut(s) 605, 645
NdeI CATATG 1 cut(s) 907
NdeII GATC 4 cut(s) 229, 424, 476, 999
NlaIII CATG 6 cut(s) 215, 329, 534, 609, 649, 663
NlaIV GGNNCC 2 cut(s) 79, 231
NmuCI GTSAC 1 cut(s) 293
PaqCI CACCTGC 1 cut(s) 952
PceI AGGCCT 1 cut(s) 224
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 2 cut(s) 16, 182
Ple19I CGATCG 1 cut(s) 1002
PleI GAGTC 1 cut(s) 103
PluTI GGCGCC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 986
PsiI TTATAA 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 2 cut(s) 79, 231
PspPI GGNCC 1 cut(s) 353
PsuI RGATCY 2 cut(s) 229, 476
PvuI CGATCG 1 cut(s) 1002
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
SaqAI TTAA 3 cut(s) 399, 986, 992
SatI GCNGC 2 cut(s) 15, 181
Sau3AI GATC 4 cut(s) 229, 424, 476, 999
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 2 cut(s) 485, 723
SfcI CTRYAG 1 cut(s) 917
SfoI GGCGCC 1 cut(s) 79
SinI GGWCC 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 392, 926
SmoI CTYRAG 2 cut(s) 392, 926
Sse9I AATT 4 cut(s) 682, 792, 987, 1085
SseBI AGGCCT 1 cut(s) 224
SsiI CCGC 6 cut(s) 145, 151, 178, 181, 187, 975
SspDI GGCGCC 1 cut(s) 77
SspMI CTAG 1 cut(s) 375
StuI AGGCCT 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 3 cut(s) 225, 605, 645
TaaI ACNGT 5 cut(s) 138, 293, 861, 921, 1021
TaiI ACGT 2 cut(s) 278, 825
TaqI TCGA 3 cut(s) 165, 427, 790
TasI AATT 4 cut(s) 682, 792, 987, 1085
TatI WGTACW 1 cut(s) 1021
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 3 cut(s) 399, 986, 992
Tru9I TTAA 3 cut(s) 399, 986, 992
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 100, 1076
TspGWI ACGGA 2 cut(s) 302, 603
TspRI CASTG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 353
VspI ATTAAT 1 cut(s) 986
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.