Rroxscaffold_3G00266730

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006071
Physical Location & Seq
Reverse (-)
59927042 .. 59932191
5150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266730.1

Sequence Viewer

Length: 1104 bp
ATGGGAGATGATGCGAAGAAGAAGAGCTCTTGTTCTGATTCGCCTACTCTGGACGCCCTACAAGCTGAAGTCACCGAAGCCATGGAGGGCGCCAAGTCGTTACAGGACTCAGCTTCCTCCTTCATCTCCAGAAACTCCAGCGAGGAACAGTCGCTTCGCCAGCGAGCCCAGTCTCTCGATTCCACAATCCGCCGCCTCCGTTCCCAAATCTATTCTCTTCATCATGACAACAAGGCCGTGGATCCCAAGCTCTGCGAAAAGCTGGAAGAAGATTTGCACAGGGCCACGTGTGTCTTGACGGACGGGGACGCCGGTTCTTTTCTTCCTCCCAAAGCGCACGGCGGGTTTCTGAGGATGTTTCTTGGTCCTATCAACGTGCGTTCGTCTAGCAAAGACATCCAACTCAAGGTTAAAGAGGAATACAATGCTTACAGAGATCGAACTGCCCTGCTATTCCTTCTCTTTCCATTTGCACTACTTATTCTGAGGTCTTGGATATGGGATGGATGCTTGCCAACATTTCCTGTTCAAGCTTACCAGGCATGGCTCTTATTCCTCTACACTGGTTTGTCATTGCGAGAAAACATACTGAGAGTAAACGGAAGTGATATTCGTCCATGGTGGATATACCATCACTATTGTGCTATGTTTATGGCCATGGTTAGTCTCACATGGGACATTCAAGGACAACCAAATTGTGCCCAAAAGCAGAGAGGGGTGGAACTTTTCCTGCAATGGGCCATGATGCAAGGAGTGGCCATGCTTTTACAAAATAGATATCAACGCCAGAGACTGTATACTCGAATTGCATTGGGAAAGGCCAATAGGATGGACGTCGTATGGGGTGAAACAGCTGGTGTTGATGGTCAATTGTGGCTTCTATGTCCCATACTTTTTATAATGCAGGGTTTTGAGGCGTATGTTGGACTACAGTTGCTTAAGACTGCTTTGTTCGGGGATGTTACCGAATGGCAGGTGAGTTTGTGCGGTGTGCTATTAATTCTTATGGCGATCGGGAACTTTGTAAATACTGTACAAACACTCATGGTGAAATCGAGGTTCAAGGCAAAGATGAAAAAGACCAAGAGCAAGCAGGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

367

Amino Acids

41.8

Weight (kDa)

9.02

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 22 - 354 1.5e-99 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 1 cut(s) 964
AatII GACGTC 1 cut(s) 837
Acc36I ACCTGC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 797
AciI CCGC 4 cut(s) 190, 193, 342, 987
AclWI GGATC 2 cut(s) 236, 249
AcoI YGGCCR 2 cut(s) 654, 756
AcuI CTGAAG 1 cut(s) 87
AcvI CACGTG 1 cut(s) 288
AcyI GRCGYC 4 cut(s) 54, 90, 309, 834
AfaI GTAC 1 cut(s) 1035
AfiI CCNNNNNNNGG 2 cut(s) 406, 736
AflII CTTAAG 1 cut(s) 938
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 3 cut(s) 530, 683, 1063
AjnI CCWGG 1 cut(s) 537
AleI CACNNNNGTG 1 cut(s) 639
AluBI AGCT 7 cut(s) 27, 65, 113, 250, 262, 533, 854
AluI AGCT 7 cut(s) 27, 65, 113, 250, 262, 533, 854
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 3 cut(s) 177, 671, 784
AlwI GGATC 2 cut(s) 236, 249
AlwNI CAGNNNCTG 1 cut(s) 793
AoxI GGCC 6 cut(s) 234, 282, 654, 738, 756, 819
AseI ATTAAT 1 cut(s) 998
AspLEI GCGC 2 cut(s) 92, 337
AspS9I GGNCC 3 cut(s) 282, 365, 738
AsuHPI GGTGA 4 cut(s) 64, 857, 988, 1060
AvaII GGWCC 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 703
BalI TGGCCA 2 cut(s) 656, 758
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 29, 169
BbrPI CACGTG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 29
BccI CCATC 4 cut(s) 497, 639, 823, 857
BceAI ACGGC 2 cut(s) 221, 355
BciT130I CCWGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 177, 671, 784
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 929
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 964
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 3 cut(s) 282, 365, 738
BmiI GGNNCC 2 cut(s) 91, 243
BmrFI CCNGG 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 497, 735
BmuI ACTGGG 1 cut(s) 163
BpmI CTGGAG 2 cut(s) 112, 121
BpuEI CTTGAG 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 288
BsaHI GRCGYC 4 cut(s) 54, 90, 309, 834
BsaJI CCNNGG 4 cut(s) 81, 237, 617, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 736
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 169, 568
Bse3DI GCAATG 2 cut(s) 572, 740
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 81, 237, 617, 657
BseGI GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BseLI CCNNNNNNNGG 2 cut(s) 406, 736
BseMI GCAATG 2 cut(s) 572, 740
BseMII CTCAG 4 cut(s) 123, 341, 476, 581
BseNI ACTGG 2 cut(s) 169, 568
BseSI GKGCMC 1 cut(s) 703
Bsh1285I CGRYCG 1 cut(s) 1014
BshFI GGCC 6 cut(s) 236, 284, 656, 740, 758, 821
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 3 cut(s) 320, 689, 870
BslI CCNNNNNNNGG 2 cut(s) 406, 736
BsmAI GTCTC 3 cut(s) 177, 671, 784
BsmFI GGGAC 3 cut(s) 320, 689, 870
BsnI GGCC 6 cut(s) 236, 284, 656, 740, 758, 821
Bsp1286I GDGCHC 3 cut(s) 29, 169, 703
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 3 cut(s) 241, 436, 1011
Bsp19I CCATGG 3 cut(s) 81, 617, 657
BspACI CCGC 4 cut(s) 190, 193, 342, 987
BspANI GGCC 6 cut(s) 236, 284, 656, 740, 758, 821
BspCNI CTCAG 4 cut(s) 122, 342, 477, 582
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 91, 243
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 2 cut(s) 236, 249
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 938
BsrDI GCAATG 2 cut(s) 572, 740
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 2 cut(s) 169, 568
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 4 cut(s) 81, 237, 617, 657
BssMI GATC 3 cut(s) 241, 436, 1011
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 4 cut(s) 54, 90, 309, 834
BssT1I CCWWGG 3 cut(s) 81, 617, 657
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 4 cut(s) 150, 795, 933, 1033
Bst6I CTCTTC 2 cut(s) 17, 222
BstACI GRCGYC 4 cut(s) 54, 90, 309, 834
BstAFI CTTAAG 1 cut(s) 938
BstAUI TGTACA 1 cut(s) 1033
BstBAI YACGTR 1 cut(s) 288
BstC8I GCNNGC 4 cut(s) 161, 165, 512, 1091
BstDEI CTNAG 4 cut(s) 109, 350, 485, 590
BstDSI CCRYGG 4 cut(s) 81, 237, 617, 657
BstF5I GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 337
BstKTI GATC 3 cut(s) 244, 439, 1014
BstMAI GTCTC 3 cut(s) 177, 671, 784
BstMBI GATC 3 cut(s) 241, 436, 1011
BstMCI CGRYCG 1 cut(s) 1014
BstMWI GCNNNNNNNGC 3 cut(s) 62, 160, 539
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 929
BstSLI GKGCMC 1 cut(s) 703
BstX2I RGATCY 1 cut(s) 241
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 798
BsuRI GGCC 6 cut(s) 236, 284, 656, 740, 758, 821
BtgI CCRYGG 4 cut(s) 81, 237, 617, 657
BtsCI GGATG 6 cut(s) 360, 396, 508, 512, 834, 964
BtsIMutI CAGTG 1 cut(s) 561
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 4 cut(s) 161, 165, 512, 1091
CaiI CAGNNNCTG 1 cut(s) 793
CciI TCATGA 1 cut(s) 223
CfoI GCGC 2 cut(s) 92, 337
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 282, 365, 738
CseI GACGC 2 cut(s) 62, 317
Csp6I GTAC 1 cut(s) 1034
CviAII CATG 9 cut(s) 82, 224, 543, 618, 658, 672, 742, 760, 1045
CviQI GTAC 1 cut(s) 1034
DdeI CTNAG 4 cut(s) 109, 350, 485, 590
DinI GGCGCC 1 cut(s) 91
DpnI GATC 3 cut(s) 243, 438, 1013
DpnII GATC 3 cut(s) 241, 436, 1011
EaeI YGGCCR 2 cut(s) 654, 756
Eam1104I CTCTTC 2 cut(s) 17, 222
EarI CTCTTC 2 cut(s) 17, 222
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 81, 617, 657
Eco24I GRGCYC 2 cut(s) 29, 169
Eco32I GATATC 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 87
Eco72I CACGTG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 779
EcoT14I CCWWGG 3 cut(s) 81, 617, 657
EcoT38I GRGCYC 2 cut(s) 29, 169
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 81, 617, 657
FaeI CATG 9 cut(s) 85, 227, 546, 621, 661, 675, 745, 763, 1048
FaqI GGGAC 3 cut(s) 320, 689, 870
FatI CATG 9 cut(s) 81, 223, 542, 617, 657, 671, 741, 759, 1044
FauI CCCGC 1 cut(s) 335
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 6 cut(s) 367, 383, 515, 519, 841, 971
FriOI GRGCYC 2 cut(s) 29, 169
Fsp4HI GCNGC 1 cut(s) 193
FspBI CTAG 1 cut(s) 387
GlaI GCGC 2 cut(s) 91, 336
GluI GCNGC 1 cut(s) 193
GsuI CTGGAG 2 cut(s) 112, 121
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 6 cut(s) 236, 284, 656, 740, 758, 821
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 62, 317
HhaI GCGC 2 cut(s) 92, 337
Hin1I GRCGYC 4 cut(s) 54, 90, 309, 834
Hin1II CATG 9 cut(s) 85, 227, 546, 621, 661, 675, 745, 763, 1048
Hin6I GCGC 2 cut(s) 90, 335
HinP1I GCGC 2 cut(s) 90, 335
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 3 cut(s) 38, 107, 179
HpaII CCGG 1 cut(s) 312
HphI GGTGA 4 cut(s) 64, 857, 988, 1060
Hpy166II GTNNAC 2 cut(s) 598, 798
Hpy188I TCNGA 3 cut(s) 37, 351, 486
Hpy188III TCNNGA 6 cut(s) 50, 129, 176, 224, 295, 1015
Hpy8I GTNNAC 2 cut(s) 598, 798
Hpy99I CGWCG 1 cut(s) 839
HpyAV CCTTC 2 cut(s) 130, 467
HpyCH4III ACNGT 4 cut(s) 150, 795, 933, 1033
HpyCH4IV ACGT 3 cut(s) 287, 375, 834
HpyCH4V TGCA 6 cut(s) 277, 473, 733, 748, 809, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 160, 539
HpyF3I CTNAG 4 cut(s) 109, 350, 485, 590
HpySE526I ACGT 3 cut(s) 287, 375, 834
Hsp92I GRCGYC 4 cut(s) 54, 90, 309, 834
Hsp92II CATG 9 cut(s) 85, 227, 546, 621, 661, 675, 745, 763, 1048
HspAI GCGC 2 cut(s) 90, 335
KasI GGCGCC 1 cut(s) 89
Kzo9I GATC 3 cut(s) 241, 436, 1011
LguI GCTCTTC 1 cut(s) 17
LweI GCATC 2 cut(s) 497, 735
MaeI CTAG 1 cut(s) 387
MaeII ACGT 3 cut(s) 287, 375, 834
MaeIII GTNAC 3 cut(s) 70, 99, 961
MalI GATC 3 cut(s) 243, 438, 1013
MboI GATC 3 cut(s) 241, 436, 1011
MboII GAAGA 7 cut(s) 28, 31, 34, 209, 278, 281, 314
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 3 cut(s) 29, 169, 703
MlsI TGGCCA 2 cut(s) 656, 758
MluCI AATT 5 cut(s) 694, 804, 869, 999, 1097
MluNI TGGCCA 2 cut(s) 656, 758
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 424, 904
Mox20I TGGCCA 2 cut(s) 656, 758
MscI TGGCCA 2 cut(s) 656, 758
MseI TTAA 3 cut(s) 411, 939, 998
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 2 cut(s) 656, 758
MspA1I CMGCKG 1 cut(s) 854
MspCI CTTAAG 1 cut(s) 938
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 539
MunI CAATTG 1 cut(s) 869
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 62, 160, 539
NarI GGCGCC 1 cut(s) 90
NcoI CCATGG 3 cut(s) 81, 617, 657
NdeII GATC 3 cut(s) 241, 436, 1011
NlaIII CATG 9 cut(s) 85, 227, 546, 621, 661, 675, 745, 763, 1048
NlaIV GGNNCC 2 cut(s) 91, 243
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 639
PagI TCATGA 1 cut(s) 223
PaqCI CACCTGC 1 cut(s) 964
PciSI GCTCTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 38, 179
PkrI GCNGC 1 cut(s) 194
Ple19I CGATCG 1 cut(s) 1014
PleI GAGTC 1 cut(s) 101
PluTI GGCGCC 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 288
PmlI CACGTG 1 cut(s) 288
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 998
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 537
PspCI CACGTG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 91, 243
PspPI GGNCC 3 cut(s) 282, 365, 738
PstNI CAGNNNCTG 1 cut(s) 793
PsuI RGATCY 1 cut(s) 241
PvuI CGATCG 1 cut(s) 1014
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 1 cut(s) 1035
RsaNI GTAC 1 cut(s) 1034
RseI CAYNNNNRTG 1 cut(s) 639
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 3 cut(s) 411, 939, 998
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 3 cut(s) 241, 436, 1011
Sau96I GGNCC 3 cut(s) 282, 365, 738
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 3 cut(s) 29, 169, 703
SfaNI GCATC 2 cut(s) 497, 735
SfcI CTRYAG 1 cut(s) 929
SfoI GGCGCC 1 cut(s) 91
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 404, 938
SmoI CTYRAG 2 cut(s) 404, 938
Sse9I AATT 5 cut(s) 694, 804, 869, 999, 1097
SsiI CCGC 4 cut(s) 190, 193, 342, 987
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 3 cut(s) 81, 617, 657
TaaI ACNGT 4 cut(s) 150, 795, 933, 1033
TaiI ACGT 3 cut(s) 290, 378, 837
TaqI TCGA 4 cut(s) 177, 439, 802, 1055
TasI AATT 5 cut(s) 694, 804, 869, 999, 1097
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 38, 179
Tru1I TTAA 3 cut(s) 411, 939, 998
Tru9I TTAA 3 cut(s) 411, 939, 998
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 112, 209, 1088
TspGWI ACGGA 3 cut(s) 188, 314, 615
TspRI CASTG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 365
VspI ATTAAT 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 1 cut(s) 387
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.