AT1G34340

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004956
Physical Location & Seq
Forward (+)
12530815 .. 12534306
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34340.1

Sequence Viewer

Length: 1593 bp
ATGGAAGAAGAAGATCTGATTCTATCTCCATCTCCATACGATCTTCTCTTTCAAGCTCTAACTCTCATCCCGATTCGTCACTACCTCATCGCTCTCTTCGTTCTCTGTACCATCTTCTTCTACAATTTCGTCGAATTTCACTTCCTTGGAGATGCGATTCTTCGTTATTTCAGATGTCGCGTCAATCTCATTTACAATCCAGATTCTCCTCTTTATCACGGTGTTGTTTCTCGGTGTCAGATTCTTCACGGCCGATATGTGGCGACGCCATGGTTAGCGAGCCCTCATATTCAGACTTGTTTCTTGAATTTCCATGGATTGCCTCCAGTTTTCACCTATACAAGACAGCTATTTCTTACTTCTGATGGTGGAACGATTGCATTAGATTGGCTTACGAATTCTGATGTTCTTGATGGTTCTCTTCACAATAAGAGTGAAATTACAAAAGAAGATACAACGCCTATAGCTGTTGTTATTCCAGGGTTAACTAGTGATTCTTCATCTGCATATTTAAAGCATCTTGCTTACGACACTGCAAAAACAGGTTGGAATGTTGTTATTAGCAACCATAGAGGATTGGGAGGAGTTTCTGTTACTTCAGATTGCTTCTATAATGCTGGATGGACAGATGATATAAGGGTAGTTCTTGATTATCTTCAACACAAATACCCGAGGGCTCCTCTCTTTGCTATTGGCACTAGCATTGGAGCAAATGTTCTGGTAAAATACCTTGGGGAAGAGGGTGAAAAGACTCCTCTGAGAGGTGCTGTTGCTATCTGCTCTCCATGGGACCTCTTGATTGGTGACAGGTTTATCTGTCGGACTTTGAAACAAAAGCTCTATGACAAAGCTCTCACCATCGGACTCCAAGGTTATGCCCAATTGCATGAACCTCAGTTTTTACGGCTTGCTAATTGGGAAGGCATCAAAAAGTCACGTTCCATCCGAGATTTTGACAACCATGCTACCTGTCTAGTTGGAAAATTCGAGACTGTGGATACGTATTACCGAAAATCGAGCAGCACTCAATACGTAGGAAATGTGGCGGTGCCACTACTCTGTATAAGTGCTCTAGATGATCCATTATGCACAAAGGAAGCTATTCCCTGGGACGAATGCAGGGCCAACAAAAACATTGTCTTGGCTACAACAAATCACGGGGGACATCTAGCGTTCTTCGAAGGATTAACTGGATCTAGCTTATGGTGGGTTCGAGCCACCAACGAGTTTCTTGGCGTCCTTAGCTGCAGTCCTTATATGCATATACAGAAAATTGTAGATAAAAGAAGCTCAGGGTCAGGGAAACAAGAGCCATCGATAAACCAAGGCCCGTACCTAAACATTGCAGAAGACGGGTTGGTGGCAGCAGTCAAATATGAGCAAGACACCAATAAGACCACACTGAAGCAGAGAGGGCGAAAGCCTGAGGAAGATGTAACAAAGAAAAGCTTTAAAGAGCTGTGTAGGCAGACAAAACAGTCGGTATGGTGGCTTGGTTACATAGGCATGGTTACAAGTTTCCCTTTACTTGGGATGCTCCTAAACTACATCTTCCGTAAGAAGCAACGACCCACAACAACATCCACATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

530

Amino Acids

59.67

Weight (kDa)

8.29

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 157 - 395 3.7e-11 alpha/beta hydrolase fold
Hydrolase_4 PF12146 157 - 263 1.6e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1477
AccB1I GGYRCC 1 cut(s) 1048
AccII CGCG 1 cut(s) 180
AciI CCGC 1 cut(s) 1046
AclWI GGATC 2 cut(s) 1073, 1201
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 4 cut(s) 134, 307, 397, 983
AcuI CTGAAG 2 cut(s) 582, 1424
AcyI GRCGYC 2 cut(s) 266, 1236
AfaI GTAC 2 cut(s) 109, 1334
AfiI CCNNNNNNNGG 3 cut(s) 259, 761, 1529
AgsI TTSAA 4 cut(s) 53, 307, 659, 829
AhlI ACTAGT 1 cut(s) 488
AjnI CCWGG 2 cut(s) 478, 1106
AloI GAACNNNNNNTCC 2 cut(s) 699, 731
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 1 cut(s) 983
AlwI GGATC 2 cut(s) 1073, 1201
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 3 cut(s) 250, 1122, 1327
ApeKI GCWGC 3 cut(s) 1020, 1245, 1364
ApoI RAATTY 4 cut(s) 134, 307, 397, 983
ArsI GACNNNNNNTTYG 2 cut(s) 1122, 1154
Asp700I GAANNNNTTC 1 cut(s) 1101
AspS9I GGNCC 3 cut(s) 790, 1122, 1328
AsuHPI GGTGA 4 cut(s) 325, 755, 815, 847
AsuII TTCGAA 1 cut(s) 1179
AvaI CYCGRG 1 cut(s) 670
AvaII GGWCC 1 cut(s) 790
AxyI CCTNAGG 1 cut(s) 1425
BanI GGYRCC 1 cut(s) 1048
BanII GRGCYC 2 cut(s) 284, 679
BbsI GAAGAC 1 cut(s) 1356
Bbv12I GWGCWC 1 cut(s) 1072
BbvI GCAGC 3 cut(s) 1032, 1232, 1376
BccI CCATC 8 cut(s) 37, 119, 359, 407, 615, 866, 950, 1321
BceAI ACGGC 2 cut(s) 265, 920
BciT130I CCWGG 2 cut(s) 480, 1108
BciVI GTATCC 1 cut(s) 991
BcoDI GTCTC 1 cut(s) 983
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 6 cut(s) 489, 699, 974, 1073, 1169, 1197
BfmI CTRYAG 2 cut(s) 462, 1246
BfuI GTATCC 1 cut(s) 991
BglII AGATCT 1 cut(s) 13
BisI GCNGC 3 cut(s) 1021, 1246, 1365
BlsI GCNGC 3 cut(s) 1022, 1247, 1366
Bme1390I CCNGG 2 cut(s) 480, 1108
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 3 cut(s) 790, 1122, 1328
BmiI GGNNCC 3 cut(s) 678, 791, 1050
BmrFI CCNGG 2 cut(s) 480, 1108
BmsI GCATC 4 cut(s) 142, 526, 933, 1524
BpiI GAAGAC 1 cut(s) 1356
BplI GAGNNNNNCTC 4 cut(s) 664, 696, 1009, 1041
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 2 cut(s) 1241, 1291
Bpu14I TTCGAA 1 cut(s) 1179
Bsa29I ATCGAT 1 cut(s) 1316
BsaAI YACGTR 2 cut(s) 1002, 1033
BsaHI GRCGYC 2 cut(s) 266, 1236
BsaXI ACNNNNNCTCC 4 cut(s) 576, 606, 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 761, 1529
Bse1I ACTGG 2 cut(s) 326, 1195
Bse21I CCTNAGG 1 cut(s) 1425
Bse3DI GCAATG 1 cut(s) 1341
BseBI CCWGG 2 cut(s) 480, 1108
BseCI ATCGAT 1 cut(s) 1316
BseGI GGATG 6 cut(s) 66, 626, 942, 1539, 1580, 1586
BseLI CCNNNNNNNGG 3 cut(s) 259, 761, 1529
BseMI GCAATG 1 cut(s) 1341
BseMII CTCAG 4 cut(s) 749, 908, 1305, 1416
BseNI ACTGG 2 cut(s) 326, 1195
BseRI GAGGAG 4 cut(s) 198, 597, 669, 744
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 3 cut(s) 1032, 1232, 1376
Bsh1236I CGCG 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 3 cut(s) 252, 1124, 1329
BshNI GGYRCC 1 cut(s) 1048
BshVI ATCGAT 1 cut(s) 1316
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 1072
BsiHKCI CYCGRG 1 cut(s) 670
BslFI GGGAC 3 cut(s) 803, 1124, 1176
BslI CCNNNNNNNGG 3 cut(s) 259, 761, 1529
BsmAI GTCTC 1 cut(s) 983
BsmFI GGGAC 3 cut(s) 803, 1124, 1176
BsmI GAATGC 1 cut(s) 1121
BsnI GGCC 3 cut(s) 252, 1124, 1329
BsoBI CYCGRG 1 cut(s) 670
Bsp119I TTCGAA 1 cut(s) 1179
Bsp1286I GDGCHC 3 cut(s) 284, 679, 1072
Bsp143I GATC 4 cut(s) 13, 40, 1078, 1193
Bsp19I CCATGG 3 cut(s) 269, 313, 785
BspACI CCGC 1 cut(s) 1046
BspANI GGCC 3 cut(s) 252, 1124, 1329
BspCNI CTCAG 4 cut(s) 750, 907, 1304, 1417
BspDI ATCGAT 1 cut(s) 1316
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 678, 791, 1050
BspMAI CTGCAG 1 cut(s) 1250
BspPI GGATC 2 cut(s) 1073, 1201
BspT104I TTCGAA 1 cut(s) 1179
BspT107I GGYRCC 1 cut(s) 1048
BsrDI GCAATG 1 cut(s) 1341
BsrI ACTGG 2 cut(s) 326, 1195
BssMI GATC 4 cut(s) 13, 40, 1078, 1193
BssNI GRCGYC 2 cut(s) 266, 1236
BssT1I CCWWGG 7 cut(s) 145, 269, 313, 730, 785, 868, 1324
Bst2UI CCWGG 2 cut(s) 480, 1108
Bst4CI ACNGT 3 cut(s) 221, 994, 1479
Bst6I CTCTTC 3 cut(s) 101, 426, 732
BstACI GRCGYC 2 cut(s) 266, 1236
BstBAI YACGTR 2 cut(s) 1002, 1033
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 2 cut(s) 280, 909
BstDEI CTNAG 5 cut(s) 758, 894, 1241, 1291, 1425
BstDSI CCRYGG 3 cut(s) 269, 313, 785
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 6 cut(s) 66, 626, 942, 1539, 1580, 1586
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 4 cut(s) 16, 43, 1081, 1196
BstMAI GTCTC 1 cut(s) 983
BstMBI GATC 4 cut(s) 13, 40, 1078, 1193
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 3 cut(s) 1242, 1414, 1465
BstNI CCWGG 2 cut(s) 480, 1108
BstSCI CCNGG 2 cut(s) 478, 1106
BstSFI CTRYAG 2 cut(s) 462, 1246
BstSNI TACGTA 2 cut(s) 1002, 1033
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 1032, 1232, 1376
BstV2I GAAGAC 1 cut(s) 1356
BstX2I RGATCY 2 cut(s) 13, 1193
BstYI RGATCY 2 cut(s) 13, 1193
BstZI CGGCCG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 1316
Bsu36I CCTNAGG 1 cut(s) 1425
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 3 cut(s) 252, 1124, 1329
BsuTUI ATCGAT 1 cut(s) 1316
BtgI CCRYGG 3 cut(s) 269, 313, 785
BtgZI GCGATG 1 cut(s) 73
BtsCI GGATG 6 cut(s) 66, 626, 942, 1539, 1580, 1586
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 531, 1400
Cac8I GCNNGC 2 cut(s) 280, 909
Cfr13I GGNCC 3 cut(s) 790, 1122, 1328
ClaI ATCGAT 1 cut(s) 1316
CseI GACGC 3 cut(s) 169, 274, 1225
Csp6I GTAC 2 cut(s) 108, 1333
CviAII CATG 6 cut(s) 270, 314, 786, 887, 962, 1507
CviQI GTAC 2 cut(s) 108, 1333
DdeI CTNAG 5 cut(s) 758, 894, 1241, 1291, 1425
DpnI GATC 4 cut(s) 15, 42, 1080, 1195
DpnII GATC 4 cut(s) 13, 40, 1078, 1193
DraI TTTAAA 2 cut(s) 513, 1453
DrdI GACNNNNNNGTC 1 cut(s) 1477
DseDI GACNNNNNNGTC 1 cut(s) 1477
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 101, 426, 732
EarI CTCTTC 3 cut(s) 101, 426, 732
EclXI CGGCCG 1 cut(s) 250
Eco105I TACGTA 2 cut(s) 1002, 1033
Eco130I CCWWGG 7 cut(s) 145, 269, 313, 730, 785, 868, 1324
Eco24I GRGCYC 2 cut(s) 284, 679
Eco47I GGWCC 1 cut(s) 790
Eco52I CGGCCG 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 582, 1424
Eco81I CCTNAGG 1 cut(s) 1425
Eco88I CYCGRG 1 cut(s) 670
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 478, 1106
EcoT14I CCWWGG 7 cut(s) 145, 269, 313, 730, 785, 868, 1324
EcoT22I ATGCAT 1 cut(s) 1263
EcoT38I GRGCYC 2 cut(s) 284, 679
ErhI CCWWGG 7 cut(s) 145, 269, 313, 730, 785, 868, 1324
FaeI CATG 6 cut(s) 273, 317, 789, 890, 965, 1510
FalI AAGNNNNNCTT 4 cut(s) 1433, 1465, 1507, 1539
FaqI GGGAC 3 cut(s) 803, 1124, 1176
FatI CATG 6 cut(s) 269, 313, 785, 886, 961, 1506
Fnu4HI GCNGC 3 cut(s) 1021, 1246, 1365
FokI GGATG 6 cut(s) 53, 633, 929, 1546, 1567, 1573
FriOI GRGCYC 2 cut(s) 284, 679
Fsp4HI GCNGC 3 cut(s) 1021, 1246, 1365
FspBI CTAG 6 cut(s) 489, 699, 974, 1073, 1169, 1197
GluI GCNGC 3 cut(s) 1021, 1246, 1365
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 3 cut(s) 252, 1124, 1329
HgaI GACGC 3 cut(s) 169, 274, 1225
Hin1I GRCGYC 2 cut(s) 266, 1236
Hin1II CATG 6 cut(s) 273, 317, 789, 890, 965, 1510
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 1 cut(s) 1447
HinfI GANTC 8 cut(s) 19, 73, 157, 203, 241, 494, 751, 864
HpaI GTTAAC 1 cut(s) 486
HphI GGTGA 4 cut(s) 325, 755, 815, 847
Hpy166II GTNNAC 1 cut(s) 486
Hpy188III TCNNGA 9 cut(s) 70, 200, 304, 410, 647, 796, 988, 1073, 1590
Hpy8I GTNNAC 1 cut(s) 486
Hpy99I CGWCG 2 cut(s) 134, 268
HpyAV CCTTC 2 cut(s) 914, 1175
HpyCH4III ACNGT 3 cut(s) 221, 994, 1479
HpyCH4IV ACGT 3 cut(s) 937, 1001, 1032
HpyCH4V TGCA 9 cut(s) 380, 506, 536, 886, 1089, 1119, 1248, 1261, 1346
HpyF10VI GCNNNNNNNGC 3 cut(s) 1242, 1414, 1465
HpyF3I CTNAG 5 cut(s) 758, 894, 1241, 1291, 1425
HpySE526I ACGT 3 cut(s) 937, 1001, 1032
Hsp92I GRCGYC 2 cut(s) 266, 1236
Hsp92II CATG 6 cut(s) 273, 317, 789, 890, 965, 1510
KspAI GTTAAC 1 cut(s) 486
Kzo9I GATC 4 cut(s) 13, 40, 1078, 1193
LmnI GCTCC 3 cut(s) 682, 707, 1542
Lsp1109I GCAGC 3 cut(s) 1032, 1232, 1376
LweI GCATC 4 cut(s) 142, 526, 933, 1524
MaeI CTAG 6 cut(s) 489, 699, 974, 1073, 1169, 1197
MaeII ACGT 3 cut(s) 937, 1001, 1032
MaeIII GTNAC 7 cut(s) 77, 592, 803, 933, 1435, 1496, 1510
MalI GATC 4 cut(s) 15, 42, 1080, 1195
MboI GATC 4 cut(s) 13, 40, 1078, 1193
MfeI CAATTG 1 cut(s) 881
MflI RGATCY 2 cut(s) 13, 1193
MhlI GDGCHC 3 cut(s) 284, 679, 1072
MluCI AATT 9 cut(s) 124, 134, 307, 397, 438, 881, 913, 983, 1272
MlyI GAGTC 2 cut(s) 745, 858
MmeI TCCRAC 3 cut(s) 527, 800, 958
Mph1103I ATGCAT 1 cut(s) 1263
MroXI GAANNNNTTC 1 cut(s) 1101
MseI TTAA 4 cut(s) 485, 512, 1187, 1452
MslI CAYNNNNRTG 1 cut(s) 1505
MspR9I CCNGG 2 cut(s) 480, 1108
MunI CAATTG 1 cut(s) 881
Mva1269I GAATGC 1 cut(s) 1121
MvaI CCWGG 2 cut(s) 480, 1108
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 3 cut(s) 1242, 1414, 1465
NcoI CCATGG 3 cut(s) 269, 313, 785
NdeII GATC 4 cut(s) 13, 40, 1078, 1193
NlaIII CATG 6 cut(s) 273, 317, 789, 890, 965, 1510
NlaIV GGNNCC 3 cut(s) 678, 791, 1050
NmuCI GTSAC 3 cut(s) 77, 803, 933
NsiI ATGCAT 1 cut(s) 1263
NspV TTCGAA 1 cut(s) 1179
PasI CCCWGGG 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 943
PctI GAATGC 1 cut(s) 1121
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 6 cut(s) 19, 73, 157, 203, 241, 494
PkrI GCNGC 3 cut(s) 1022, 1247, 1366
PleI GAGTC 2 cut(s) 745, 858
PpsI GAGTC 2 cut(s) 745, 858
Ppu21I YACGTR 2 cut(s) 1002, 1033
PpuMI RGGWCCY 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 478, 1106
PspGI CCWGG 2 cut(s) 478, 1106
PspN4I GGNNCC 3 cut(s) 678, 791, 1050
PspPI GGNCC 3 cut(s) 790, 1122, 1328
PspPPI RGGWCCY 1 cut(s) 790
PstI CTGCAG 1 cut(s) 1250
PsuI RGATCY 2 cut(s) 13, 1193
RsaI GTAC 2 cut(s) 109, 1334
RsaNI GTAC 2 cut(s) 108, 1333
RseI CAYNNNNRTG 1 cut(s) 1505
SaqAI TTAA 4 cut(s) 485, 512, 1187, 1452
SatI GCNGC 3 cut(s) 1021, 1246, 1365
Sau3AI GATC 4 cut(s) 13, 40, 1078, 1193
Sau96I GGNCC 3 cut(s) 790, 1122, 1328
SchI GAGTC 2 cut(s) 745, 858
ScrFI CCNGG 2 cut(s) 480, 1108
SduI GDGCHC 3 cut(s) 284, 679, 1072
SfaNI GCATC 4 cut(s) 142, 526, 933, 1524
SfcI CTRYAG 2 cut(s) 462, 1246
SfuI TTCGAA 1 cut(s) 1179
SinI GGWCC 1 cut(s) 790
SmiMI CAYNNNNRTG 1 cut(s) 1505
SnaBI TACGTA 2 cut(s) 1002, 1033
SpeI ACTAGT 1 cut(s) 488
Sse9I AATT 9 cut(s) 124, 134, 307, 397, 438, 881, 913, 983, 1272
SsiI CCGC 1 cut(s) 1046
SspMI CTAG 6 cut(s) 489, 699, 974, 1073, 1169, 1197
StyD4I CCNGG 2 cut(s) 478, 1106
StyI CCWWGG 7 cut(s) 145, 269, 313, 730, 785, 868, 1324
TaaI ACNGT 3 cut(s) 221, 994, 1479
TaiI ACGT 3 cut(s) 940, 1004, 1035
TaqI TCGA 6 cut(s) 132, 987, 1016, 1179, 1213, 1316
TasI AATT 9 cut(s) 124, 134, 307, 397, 438, 881, 913, 983, 1272
TfiI GAWTC 6 cut(s) 19, 73, 157, 203, 241, 494
Tru1I TTAA 4 cut(s) 485, 512, 1187, 1452
Tru9I TTAA 4 cut(s) 485, 512, 1187, 1452
TscAI CASTG 2 cut(s) 538, 1407
TseFI GTSAC 3 cut(s) 77, 803, 933
TseI GCWGC 3 cut(s) 1020, 1245, 1364
Tsp45I GTSAC 3 cut(s) 77, 803, 933
TspDTI ATGAA 2 cut(s) 489, 903
TspGWI ACGGA 1 cut(s) 1544
TspRI CASTG 2 cut(s) 538, 1407
VpaK11BI GGWCC 1 cut(s) 790
XagI CCTNNNNNAGG 1 cut(s) 759
XapI RAATTY 4 cut(s) 134, 307, 397, 983
XbaI TCTAGA 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 6 cut(s) 489, 699, 974, 1073, 1169, 1197
Zsp2I ATGCAT 1 cut(s) 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.