MD13G1206800.v1.1

Embryogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004956
Physical Location & Seq
Forward (+)
18875027 .. 18877288
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1206800.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGACTGTTTTCGGAGCCTCCATGACTCTTCCGCTAACCCCTACGAGCTTCTGTTCACCGCCGCGGCACTCATTCCCGTCTGGCACTCGTTAGGGTCCCTCATCATTTCCTGTGTGTTCCTCTACAACTACTCGGACTTCCATTTTTGGCAAGACTTTTTCACTGGATTCAGAGGTTCTCCAGTGGACTTGACATACAGCTCCAATTCCCACATCTACCGCGGTGTTGTTTCCAAGTGCCGGATACTCCATGCCCGGTACTTGGCCACGCCCTGGCTTGCCAGCCCTCATCTGCAGAATCTTTTCCTCAGTTTTTTTGGCAACACTCCAGATGTCACATACCGAAGAGAAATTTTTCGCCTGTCGGATGGCTGTACTATTGCATTGGATTGGGCAGCGGCGGCAAGTTCTCATGTTTTGGGTGGTAGTTTTGTGCCTGTAGATGACAGCACAAACCCCATTGTAGTTGTGCTTCCGGGACTAACAAGCGATTCGGATTCGGCTTATATAAGACATCTTGCTTTCAACACAGCAAAAATGGGATGGAATGTAGTGATCAGCAACCACAGAGGACTTGGTGGAGTTCCAATTACTTCAGACTTTATTTACAATTGTGGATGGACCAATGATATTCGCGAAGTCTGTAATCACCTCCACCATAAGCATCCCAAGGCTCCATTATTTCTCATTGGAACAAGCATAGGTGCCAACATTTTGGTGAAGTATCTTGGGGAAGACGGAAGCAATGTCCCGACCGTTGACACATTTTATCGGTGCACTAGCAGCGCCGTCTACGTTTGCAATGTGGCGGTTCCCTTGCTTTGTATCAGCGCGTTGGACGATCCAATCTGCACCAGGGAATCCATTCCTTGGGATGAATGTAGGGACAATGTGAATGTTGTTTTGGCCACCACACAGCATCGCGGTCACTTGGCGTTTTTCGAAGGCTTCAATGCTTCTGGGATCTGGTGGGTGAGTGCTGTGGATGAGTTTTTCAAGGTTCTGAACTGTAGCGAGTATAAGCATGTAAAGAAGAAGATGAAGAGTACTTCTGGGGCAGAATTGTCATTGAACTTCACTACACCTAGGGTTGCAGAAGATGGAATTGGAGTGGTGGTTCAATTGGTGAAGGTAGCAGCATTTGTTAAGGGAGTTGCATTGGCCAAAGTTGTTGCCCAGAAAATAGGGATTGGGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

400

Amino Acids

43.94

Weight (kDa)

6.75

Isoelectric Point (pI)

26.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 152 - 247 4.6e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 2 cut(s) 273, 870
AccI GTMKAC 1 cut(s) 792
AccII CGCG 5 cut(s) 65, 222, 636, 833, 924
AclWI GGATC 2 cut(s) 836, 971
AcoI YGGCCR 3 cut(s) 264, 906, 1161
AcsI RAATTY 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 3 cut(s) 260, 376, 1048
AfiI CCNNNNNNNGG 4 cut(s) 240, 262, 273, 870
AgsI TTSAA 5 cut(s) 526, 952, 997, 1072, 1121
AjnI CCWGG 2 cut(s) 272, 854
AjuI GAANNNNNNNTTGG 4 cut(s) 887, 919, 1089, 1121
AloI GAACNNNNNNTCC 2 cut(s) 1101, 1133
AluBI AGCT 2 cut(s) 49, 201
AluI AGCT 2 cut(s) 49, 201
Alw21I GWGCWC 1 cut(s) 779
Alw44I GTGCAC 1 cut(s) 775
AlwI GGATC 2 cut(s) 836, 971
AoxI GGCC 4 cut(s) 264, 906, 1161, 1194
ApaLI GTGCAC 1 cut(s) 775
ApeKI GCWGC 3 cut(s) 395, 783, 1136
ApoI RAATTY 1 cut(s) 351
Asp700I GAANNNNTTC 3 cut(s) 302, 354, 864
AspA2I CCTAGG 1 cut(s) 1085
AspLEI GCGC 2 cut(s) 788, 833
AspS9I GGNCC 3 cut(s) 96, 621, 1194
AsuC2I CCSGG 2 cut(s) 256, 477
AsuHPI GGTGA 5 cut(s) 49, 641, 730, 985, 1138
AsuII TTCGAA 1 cut(s) 942
AvaII GGWCC 2 cut(s) 96, 621
AvrII CCTAGG 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 779
BalI TGGCCA 3 cut(s) 266, 908, 1163
BanI GGYRCC 1 cut(s) 704
BarI GAAGNNNNNNTAC 2 cut(s) 456, 488
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 779
BbvI GCAGC 3 cut(s) 407, 795, 1148
BccI CCATC 4 cut(s) 362, 537, 612, 1094
BceAI ACGGC 1 cut(s) 773
BciT130I CCWGG 2 cut(s) 274, 856
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 2 cut(s) 256, 477
BfaI CTAG 2 cut(s) 780, 1086
BfmI CTRYAG 3 cut(s) 293, 438, 1009
BfoI RGCGCY 1 cut(s) 789
BfuI GTATCC 1 cut(s) 237
BisI GCNGC 7 cut(s) 63, 66, 396, 399, 402, 784, 1137
BlnI CCTAGG 1 cut(s) 1085
BlsI GCNGC 7 cut(s) 64, 67, 397, 400, 403, 785, 1138
BmcAI AGTACT 1 cut(s) 1048
Bme1390I CCNGG 4 cut(s) 256, 274, 477, 856
Bme18I GGWCC 2 cut(s) 96, 621
BmgT120I GGNCC 3 cut(s) 96, 621, 1194
BmiI GGNNCC 7 cut(s) 17, 97, 98, 675, 706, 813, 1195
BmrFI CCNGG 4 cut(s) 256, 274, 477, 856
BmsI GCATC 2 cut(s) 673, 928
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 2 cut(s) 165, 312
Bpu14I TTCGAA 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 256, 477
BsaJI CCNNGG 7 cut(s) 63, 220, 272, 669, 855, 869, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 1101, 1131
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 262, 273, 870
Bse1I ACTGG 2 cut(s) 169, 182
Bse3DI GCAATG 2 cut(s) 751, 808
BseBI CCWGG 2 cut(s) 274, 856
BseDI CCNNGG 7 cut(s) 63, 220, 272, 669, 855, 869, 1085
BseGI GGATG 6 cut(s) 373, 548, 623, 664, 880, 991
BseLI CCNNNNNNNGG 4 cut(s) 240, 262, 273, 870
BseMI GCAATG 2 cut(s) 751, 808
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 2 cut(s) 169, 182
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 3 cut(s) 407, 795, 1148
BsgI GTGCAG 1 cut(s) 835
Bsh1236I CGCG 5 cut(s) 65, 222, 636, 833, 924
Bsh1285I CGRYCG 1 cut(s) 756
BshFI GGCC 4 cut(s) 266, 908, 1163, 1196
BshNI GGYRCC 1 cut(s) 704
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 779
BsiSI CCGG 3 cut(s) 241, 256, 476
BslFI GGGAC 4 cut(s) 82, 492, 734, 899
BslI CCNNNNNNNGG 4 cut(s) 240, 262, 273, 870
BsmFI GGGAC 4 cut(s) 82, 492, 734, 899
BsnI GGCC 4 cut(s) 266, 908, 1163, 1196
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 1 cut(s) 779
Bsp143I GATC 3 cut(s) 555, 841, 963
Bsp68I TCGCGA 1 cut(s) 636
BspANI GGCC 4 cut(s) 266, 908, 1163, 1196
BspCNI CTCAG 1 cut(s) 321
BspFNI CGCG 5 cut(s) 65, 222, 636, 833, 924
BspLI GGNNCC 7 cut(s) 17, 97, 98, 675, 706, 813, 1195
BspMAI CTGCAG 1 cut(s) 297
BspPI GGATC 2 cut(s) 836, 971
BspT104I TTCGAA 1 cut(s) 942
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 751, 808
BsrI ACTGG 2 cut(s) 169, 182
BssECI CCNNGG 7 cut(s) 63, 220, 272, 669, 855, 869, 1085
BssMI GATC 3 cut(s) 555, 841, 963
BssT1I CCWWGG 3 cut(s) 669, 869, 1085
Bst2UI CCWGG 2 cut(s) 274, 856
Bst4CI ACNGT 3 cut(s) 8, 757, 1010
Bst6I CTCTTC 3 cut(s) 34, 340, 1037
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 2 cut(s) 279, 283
BstDEI CTNAG 1 cut(s) 308
BstDSI CCRYGG 2 cut(s) 63, 220
BstF5I GGATG 6 cut(s) 373, 548, 623, 664, 880, 991
BstFNI CGCG 5 cut(s) 65, 222, 636, 833, 924
BstH2I RGCGCY 1 cut(s) 789
BstHHI GCGC 2 cut(s) 788, 833
BstKTI GATC 3 cut(s) 558, 844, 966
BstMBI GATC 3 cut(s) 555, 841, 963
BstMCI CGRYCG 1 cut(s) 756
BstMWI GCNNNNNNNGC 2 cut(s) 401, 783
BstNI CCWGG 2 cut(s) 274, 856
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 4 cut(s) 254, 272, 475, 854
BstSFI CTRYAG 3 cut(s) 293, 438, 1009
BstSLI GKGCMC 1 cut(s) 779
BstUI CGCG 5 cut(s) 65, 222, 636, 833, 924
BstV1I GCAGC 3 cut(s) 407, 795, 1148
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 1 cut(s) 963
BstXI CCANNNNNNTGG 1 cut(s) 715
BstYI RGATCY 1 cut(s) 963
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 4 cut(s) 266, 908, 1163, 1196
BtgI CCRYGG 2 cut(s) 63, 220
BtgZI GCGATG 1 cut(s) 905
BtsCI GGATG 6 cut(s) 373, 548, 623, 664, 880, 991
BtsIMutI CAGTG 2 cut(s) 162, 189
BtuMI TCGCGA 1 cut(s) 636
Cac8I GCNNGC 2 cut(s) 279, 283
CfoI GCGC 2 cut(s) 788, 833
Cfr13I GGNCC 3 cut(s) 96, 621, 1194
Cfr42I CCGCGG 2 cut(s) 66, 223
Csp6I GTAC 3 cut(s) 259, 375, 1047
CviAII CATG 4 cut(s) 23, 251, 413, 1025
CviQI GTAC 3 cut(s) 259, 375, 1047
DdeI CTNAG 1 cut(s) 308
DpnI GATC 3 cut(s) 557, 843, 965
DpnII GATC 3 cut(s) 555, 841, 963
EaeI YGGCCR 3 cut(s) 264, 906, 1161
Eam1104I CTCTTC 3 cut(s) 34, 340, 1037
EarI CTCTTC 3 cut(s) 34, 340, 1037
Eco130I CCWWGG 3 cut(s) 669, 869, 1085
Eco47I GGWCC 2 cut(s) 96, 621
Eco57I CTGAAG 1 cut(s) 579
EcoO109I RGGNCCY 2 cut(s) 96, 1194
EcoRII CCWGG 2 cut(s) 272, 854
EcoT14I CCWWGG 3 cut(s) 669, 869, 1085
ErhI CCWWGG 3 cut(s) 669, 869, 1085
FaeI CATG 4 cut(s) 26, 254, 416, 1028
FaqI GGGAC 4 cut(s) 82, 492, 734, 899
FatI CATG 4 cut(s) 22, 250, 412, 1024
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 63, 66, 396, 399, 402, 784, 1137
FokI GGATG 6 cut(s) 380, 555, 630, 651, 887, 998
Fsp4HI GCNGC 7 cut(s) 63, 66, 396, 399, 402, 784, 1137
FspBI CTAG 2 cut(s) 780, 1086
GlaI GCGC 2 cut(s) 787, 832
GluI GCNGC 7 cut(s) 63, 66, 396, 399, 402, 784, 1137
GsuI CTGGAG 2 cut(s) 165, 312
HaeII RGCGCY 1 cut(s) 789
HaeIII GGCC 4 cut(s) 266, 908, 1163, 1196
HapII CCGG 3 cut(s) 241, 256, 476
HhaI GCGC 2 cut(s) 788, 833
Hin1II CATG 4 cut(s) 26, 254, 416, 1028
Hin6I GCGC 2 cut(s) 786, 831
HinP1I GCGC 2 cut(s) 786, 831
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 6 cut(s) 26, 168, 298, 491, 497, 860
HpaII CCGG 3 cut(s) 241, 256, 476
HphI GGTGA 5 cut(s) 49, 641, 730, 985, 1138
Hpy166II GTNNAC 5 cut(s) 57, 187, 760, 777, 793
Hpy188I TCNGA 7 cut(s) 15, 136, 173, 367, 496, 598, 1005
Hpy188III TCNNGA 3 cut(s) 329, 635, 751
Hpy8I GTNNAC 5 cut(s) 57, 187, 760, 777, 793
HpyAV CCTTC 2 cut(s) 938, 1123
HpyCH4III ACNGT 3 cut(s) 8, 757, 1010
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 7 cut(s) 295, 383, 777, 801, 852, 1094, 1157
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 783
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 1 cut(s) 795
Hsp92II CATG 4 cut(s) 26, 254, 416, 1028
HspAI GCGC 2 cut(s) 786, 831
KflI GGGWCCC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 555
KspI CCGCGG 2 cut(s) 66, 223
Kzo9I GATC 3 cut(s) 555, 841, 963
LmnI GCTCC 3 cut(s) 15, 206, 679
Lsp1109I GCAGC 3 cut(s) 407, 795, 1148
LweI GCATC 2 cut(s) 673, 928
MaeI CTAG 2 cut(s) 780, 1086
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 2 cut(s) 334, 926
MalI GATC 3 cut(s) 557, 843, 965
MboI GATC 3 cut(s) 555, 841, 963
MboII GAAGA 7 cut(s) 21, 357, 746, 1045, 1048, 1054, 1109
MfeI CAATTG 2 cut(s) 610, 1121
MflI RGATCY 1 cut(s) 963
MhlI GDGCHC 1 cut(s) 779
MlsI TGGCCA 3 cut(s) 266, 908, 1163
MluCI AATT 7 cut(s) 205, 351, 588, 610, 1061, 1104, 1121
MluNI TGGCCA 3 cut(s) 266, 908, 1163
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 345, 816
MnlI CCTC 8 cut(s) 29, 110, 131, 167, 297, 317, 563, 662
Mox20I TGGCCA 3 cut(s) 266, 908, 1163
MroXI GAANNNNTTC 3 cut(s) 302, 354, 864
MscI TGGCCA 3 cut(s) 266, 908, 1163
MseI TTAA 1 cut(s) 1146
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 3 cut(s) 266, 908, 1163
MspA1I CMGCKG 3 cut(s) 65, 222, 398
MspI CCGG 3 cut(s) 241, 256, 476
MspR9I CCNGG 4 cut(s) 256, 274, 477, 856
MunI CAATTG 2 cut(s) 610, 1121
MvaI CCWGG 2 cut(s) 274, 856
MvnI CGCG 5 cut(s) 65, 222, 636, 833, 924
MwoI GCNNNNNNNGC 2 cut(s) 401, 783
NciI CCSGG 2 cut(s) 256, 477
NdeII GATC 3 cut(s) 555, 841, 963
NlaIII CATG 4 cut(s) 26, 254, 416, 1028
NlaIV GGNNCC 7 cut(s) 17, 97, 98, 675, 706, 813, 1195
NmuCI GTSAC 2 cut(s) 334, 926
NruI TCGCGA 1 cut(s) 636
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 942
PdmI GAANNNNTTC 3 cut(s) 302, 354, 864
PfeI GAWTC 5 cut(s) 168, 298, 491, 497, 860
PflMI CCANNNNNTGG 2 cut(s) 273, 870
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 7 cut(s) 64, 67, 397, 400, 403, 785, 1138
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 272, 854
PspGI CCWGG 2 cut(s) 272, 854
PspN4I GGNNCC 7 cut(s) 17, 97, 98, 675, 706, 813, 1195
PspPI GGNCC 3 cut(s) 96, 621, 1194
PspPPI RGGWCCY 1 cut(s) 96
PstI CTGCAG 1 cut(s) 297
PsuI RGATCY 1 cut(s) 963
RruI TCGCGA 1 cut(s) 636
RsaI GTAC 3 cut(s) 260, 376, 1048
RsaNI GTAC 3 cut(s) 259, 375, 1047
RseI CAYNNNNRTG 1 cut(s) 716
SacII CCGCGG 2 cut(s) 66, 223
SaqAI TTAA 1 cut(s) 1146
SatI GCNGC 7 cut(s) 63, 66, 396, 399, 402, 784, 1137
Sau3AI GATC 3 cut(s) 555, 841, 963
Sau96I GGNCC 3 cut(s) 96, 621, 1194
ScaI AGTACT 1 cut(s) 1048
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 4 cut(s) 256, 274, 477, 856
SduI GDGCHC 1 cut(s) 779
SetI ASST 9 cut(s) 51, 178, 203, 654, 706, 798, 1002, 1087, 1134
SfaNI GCATC 2 cut(s) 673, 928
SfcI CTRYAG 3 cut(s) 293, 438, 1009
Sfr303I CCGCGG 2 cut(s) 66, 223
SfuI TTCGAA 1 cut(s) 942
SgrBI CCGCGG 2 cut(s) 66, 223
SinI GGWCC 2 cut(s) 96, 621
SmiMI CAYNNNNRTG 1 cut(s) 716
Sse9I AATT 7 cut(s) 205, 351, 588, 610, 1061, 1104, 1121
SspMI CTAG 2 cut(s) 780, 1086
StyD4I CCNGG 4 cut(s) 254, 272, 475, 854
StyI CCWWGG 3 cut(s) 669, 869, 1085
TaaI ACNGT 3 cut(s) 8, 757, 1010
TaiI ACGT 1 cut(s) 798
TaqI TCGA 1 cut(s) 942
TasI AATT 7 cut(s) 205, 351, 588, 610, 1061, 1104, 1121
TatI WGTACW 2 cut(s) 374, 1046
TauI GCSGC 4 cut(s) 65, 68, 401, 404
TfiI GAWTC 5 cut(s) 168, 298, 491, 497, 860
Tru1I TTAA 1 cut(s) 1146
Tru9I TTAA 1 cut(s) 1146
TscAI CASTG 2 cut(s) 169, 189
TseFI GTSAC 2 cut(s) 334, 926
TseI GCWGC 3 cut(s) 395, 783, 1136
Tsp45I GTSAC 2 cut(s) 334, 926
TspDTI ATGAA 2 cut(s) 891, 1055
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 2 cut(s) 169, 189
Van91I CCANNNNNTGG 2 cut(s) 273, 870
VneI GTGCAC 1 cut(s) 775
VpaK11BI GGWCC 2 cut(s) 96, 621
XapI RAATTY 1 cut(s) 351
XceI RCATGY 1 cut(s) 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmaJI CCTAGG 1 cut(s) 1085
XmiI GTMKAC 1 cut(s) 792
XmnI GAANNNNTTC 3 cut(s) 302, 354, 864
XspI CTAG 2 cut(s) 780, 1086
ZrmI AGTACT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.