FvH4_4g02600

Embryogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004956
Physical Location & Seq
Forward (+)
2351951 .. 2353725
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02600.t1

Sequence Viewer

Length: 975 bp
ATGCCTAGAGATGATGCGTCTCCAATTTTAATGGTGCTTCCAGGACTAACCAGTGATTCTAACTCTTCTTACATTAGACACCTTACCTGCAACACATCTAAGTTGTTGGGCTGGAATGTAGTTGTTAGCAACCACAGAGGACTTGGTGGAGTTCCAATCACTTCAGACTTCATTTACACTTGTGGATGGACTAATGATGTACGTGAAGTCACTAATCATCTTCATCATAGACATCCTAACGCTCCATTGTTCATAGTCGGAACAAGCATAGGTGCCAACATATTGGTCAAGTATCTAGGGGAAGATGGAGAAAATGTTCCCGTTTCTGGTGCTGCAGCCATTTGTTGTCCTTGGGATTTTCTTGTTGGTGATAGATTCATAAGACGCACTCTTCTGCAAAGGTTATACGATAAAGCTCTAACGCTTGGCCTTCAATGGTATTCAGAACAACACAGACATTCCTATTCCCGCCTCTCTGATTGGGAAGGCAAGATGCATAGTATTAGGCATTACCACAAGACATGCGATGTGGGTAAGTTTGAGACTGTGGATGCATTTTACAGGCGCAATAGTAGTGCCATTTACGTATGTAACGTTTCTGTGCCACTGCTTTGCATCAACGCTTTGGATGATCCACTTTGTACCGCAGAAGCCATTCCATGGGACGAGTGTAGAGAAAATGTGAATGTTGTGTTGGCTACAACAACGCATGGGAGTCACTTGGCTTACTTTGAACGTATTACTGCTTCAGGGATTTGGTGGGTGAGAGCAGTGGATGAGTTCTTTAGTGTTCTGAATTGTAGTGAGTTTAAGCATAAGCAAAAGGATTTAGGGGCAGAATCTACATTGGACTCTACGCTTGATCATGATCATTCATCGCCTAATTTGATGGTTGCATTAGTGAAGGTGGTTGCATTAGTGAAGCTGGTTGCATTGGTTAAAGCAGGGTTTAGGAAATGGGGATTTTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

325

Amino Acids

36.33

Weight (kDa)

6.75

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 31 - 218 5.4e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 660
AciI CCGC 2 cut(s) 469, 645
AclI AACGTT 1 cut(s) 594
AclWI GGATC 1 cut(s) 626
AcuI CTGAAG 2 cut(s) 147, 732
AfaI GTAC 2 cut(s) 201, 643
AfiI CCNNNNNNNGG 2 cut(s) 326, 660
AgsI TTSAA 2 cut(s) 434, 734
AjnI CCWGG 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 2 cut(s) 416, 925
AluI AGCT 2 cut(s) 416, 925
Alw26I GTCTC 2 cut(s) 24, 536
AlwI GGATC 1 cut(s) 626
AoxI GGCC 2 cut(s) 427, 969
ApeKI GCWGC 2 cut(s) 332, 335
Asp700I GAANNNNTTC 2 cut(s) 315, 654
AspLEI GCGC 1 cut(s) 567
AsuHPI GGTGA 2 cut(s) 380, 775
BanI GGYRCC 1 cut(s) 272
BarI GAAGNNNNNNTAC 2 cut(s) 730, 762
BbvI GCAGC 2 cut(s) 319, 347
BccI CCATC 3 cut(s) 180, 299, 883
BciT130I CCWGG 1 cut(s) 42
BclI TGATCA 2 cut(s) 862, 868
BcoDI GTCTC 2 cut(s) 24, 536
BfaI CTAG 2 cut(s) 6, 296
BfmI CTRYAG 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 2 cut(s) 333, 336
BlsI GCNGC 2 cut(s) 334, 337
Bme1390I CCNGG 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 4 cut(s) 4, 483, 541, 624
BsaAI YACGTR 2 cut(s) 203, 586
BsaBI GATNNNNATC 1 cut(s) 867
BsaJI CCNNGG 2 cut(s) 350, 659
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 660
Bse1I ACTGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 350, 659
BseGI GGATG 5 cut(s) 191, 232, 556, 634, 781
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 2 cut(s) 326, 660
BseNI ACTGG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 319, 347
BshFI GGCC 2 cut(s) 429, 971
BshNI GGYRCC 1 cut(s) 272
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 2 cut(s) 326, 660
BsmAI GTCTC 2 cut(s) 24, 536
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 2 cut(s) 429, 971
Bsp143I GATC 3 cut(s) 631, 862, 868
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 2 cut(s) 469, 645
BspANI GGCC 2 cut(s) 429, 971
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 1 cut(s) 274
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 350, 659
BssMI GATC 3 cut(s) 631, 862, 868
BssT1I CCWWGG 2 cut(s) 350, 659
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 2 cut(s) 70, 396
BstBAI YACGTR 2 cut(s) 203, 586
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 5 cut(s) 191, 232, 556, 634, 781
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 3 cut(s) 634, 865, 871
BstMAI GTCTC 2 cut(s) 24, 536
BstMBI GATC 3 cut(s) 631, 862, 868
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 525
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 333
BstSNI TACGTA 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 319, 347
BstXI CCANNNNNNTGG 1 cut(s) 283
BsuRI GGCC 2 cut(s) 429, 971
BtgI CCRYGG 1 cut(s) 659
BtgZI GCGATG 2 cut(s) 540, 861
BtsCI GGATG 5 cut(s) 191, 232, 556, 634, 781
BtsI GCAGTG 2 cut(s) 605, 777
BtsIMutI CAGTG 3 cut(s) 58, 605, 777
BveI ACCTGC 1 cut(s) 95
CciI TCATGA 1 cut(s) 865
CfoI GCGC 1 cut(s) 567
CseI GACGC 2 cut(s) 6, 393
Csp6I GTAC 2 cut(s) 200, 642
CviAII CATG 4 cut(s) 522, 660, 710, 866
CviJI RGCY 9 cut(s) 111, 338, 416, 429, 653, 698, 725, 925, 971
CviKI_1 RGCY 9 cut(s) 111, 338, 416, 429, 653, 698, 725, 925, 971
CviQI GTAC 2 cut(s) 200, 642
DdeI CTNAG 1 cut(s) 99
DpnI GATC 3 cut(s) 633, 864, 870
DpnII GATC 3 cut(s) 631, 862, 868
Eam1104I CTCTTC 2 cut(s) 70, 396
EarI CTCTTC 2 cut(s) 70, 396
Eco105I TACGTA 1 cut(s) 586
Eco130I CCWWGG 2 cut(s) 350, 659
Eco57I CTGAAG 2 cut(s) 147, 732
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 350, 659
EcoT22I ATGCAT 2 cut(s) 498, 556
ErhI CCWWGG 2 cut(s) 350, 659
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 4 cut(s) 525, 663, 713, 869
FaqI GGGAC 1 cut(s) 677
FatI CATG 4 cut(s) 521, 659, 709, 865
FauI CCCGC 1 cut(s) 476
FbaI TGATCA 2 cut(s) 862, 868
Fnu4HI GCNGC 2 cut(s) 333, 336
FokI GGATG 5 cut(s) 198, 219, 563, 641, 788
Fsp4HI GCNGC 2 cut(s) 333, 336
FspBI CTAG 2 cut(s) 6, 296
GlaI GCGC 1 cut(s) 566
GluI GCNGC 2 cut(s) 333, 336
HaeIII GGCC 2 cut(s) 429, 971
HgaI GACGC 2 cut(s) 6, 393
HhaI GCGC 1 cut(s) 567
Hin1II CATG 4 cut(s) 525, 663, 713, 869
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HinfI GANTC 5 cut(s) 56, 375, 715, 839, 851
HphI GGTGA 2 cut(s) 380, 775
Hpy188I TCNGA 5 cut(s) 166, 260, 445, 478, 795
Hpy188III TCNNGA 1 cut(s) 866
HpyAV CCTTC 3 cut(s) 440, 479, 898
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 4 cut(s) 202, 585, 594, 736
HpyCH4V TGCA 9 cut(s) 90, 335, 397, 496, 554, 615, 896, 914, 932
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 4 cut(s) 202, 585, 594, 736
Hsp92II CATG 4 cut(s) 525, 663, 713, 869
HspAI GCGC 1 cut(s) 565
Ksp22I TGATCA 2 cut(s) 862, 868
Kzo9I GATC 3 cut(s) 631, 862, 868
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 319, 347
LweI GCATC 4 cut(s) 4, 483, 541, 624
MaeI CTAG 2 cut(s) 6, 296
MaeII ACGT 4 cut(s) 202, 585, 594, 736
MaeIII GTNAC 3 cut(s) 208, 590, 716
MalI GATC 3 cut(s) 633, 864, 870
MboI GATC 3 cut(s) 631, 862, 868
MboII GAAGA 4 cut(s) 57, 212, 314, 383
MluCI AATT 3 cut(s) 24, 796, 883
MlyI GAGTC 2 cut(s) 724, 845
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 2 cut(s) 131, 482
Mph1103I ATGCAT 2 cut(s) 498, 556
MroXI GAANNNNTTC 2 cut(s) 315, 654
MseI TTAA 3 cut(s) 29, 810, 939
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 659
NdeII GATC 3 cut(s) 631, 862, 868
NlaIII CATG 4 cut(s) 525, 663, 713, 869
NlaIV GGNNCC 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 208, 716
NsiI ATGCAT 2 cut(s) 498, 556
NspI RCATGY 1 cut(s) 525
PagI TCATGA 1 cut(s) 865
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PdmI GAANNNNTTC 2 cut(s) 315, 654
PfeI GAWTC 3 cut(s) 56, 375, 839
PflMI CCANNNNNTGG 1 cut(s) 660
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 2 cut(s) 334, 337
PleI GAGTC 2 cut(s) 723, 845
PpsI GAGTC 2 cut(s) 723, 845
Ppu21I YACGTR 2 cut(s) 203, 586
Psp1406I AACGTT 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 337
RsaI GTAC 2 cut(s) 201, 643
RsaNI GTAC 2 cut(s) 200, 642
SaqAI TTAA 3 cut(s) 29, 810, 939
SatI GCNGC 2 cut(s) 333, 336
Sau3AI GATC 3 cut(s) 631, 862, 868
SchI GAGTC 2 cut(s) 724, 845
ScrFI CCNGG 1 cut(s) 42
SfaNI GCATC 4 cut(s) 4, 483, 541, 624
SfcI CTRYAG 1 cut(s) 333
SnaBI TACGTA 1 cut(s) 586
Sse9I AATT 3 cut(s) 24, 796, 883
SsiI CCGC 2 cut(s) 469, 645
SspMI CTAG 2 cut(s) 6, 296
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 2 cut(s) 350, 659
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 4 cut(s) 205, 588, 597, 739
TasI AATT 3 cut(s) 24, 796, 883
TfiI GAWTC 3 cut(s) 56, 375, 839
Tru1I TTAA 3 cut(s) 29, 810, 939
Tru9I TTAA 3 cut(s) 29, 810, 939
TscAI CASTG 3 cut(s) 58, 612, 777
TseFI GTSAC 2 cut(s) 208, 716
TseI GCWGC 2 cut(s) 332, 335
Tsp45I GTSAC 2 cut(s) 208, 716
TspDTI ATGAA 5 cut(s) 160, 212, 241, 367, 864
TspRI CASTG 3 cut(s) 58, 612, 777
Van91I CCANNNNNTGG 1 cut(s) 660
XceI RCATGY 1 cut(s) 525
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmnI GAANNNNTTC 2 cut(s) 315, 654
XspI CTAG 2 cut(s) 6, 296
Zsp2I ATGCAT 2 cut(s) 498, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.