AT5G49950

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004956
Physical Location & Seq
Reverse (-)
20319733 .. 20323649
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49950.1

Sequence Viewer

Length: 1614 bp
ATGAATTGCGCAATCACAGAGGATGATTTGATCTCTCCTTACCTCATCATCTTCAAATCGCTCTTACTCATCCCAATCTCTCACTATCTCATCTTCTTCTTCCTCACAATCTTACTTTTCCTCTACTTCTTCCTAGAGATTCATTTTCCTAAAGCAGATCCTATCTCTCTTACCTTCAATCCAAACTCTGACTTCTGCAAGTTTATTGTCTCCAAGTGTCGTCTCCTTCATGGACGATACTTTCCTACGTTCTGGCTTTCAAGCCCTCATCTTCAAACAGCTTTCCTTACCCTTTTTGGAAAGTCTCCTCCTTTCTCCTATAAGCGAATTCTCTATCAGGCAACAGATGGTGGCACAATTGCTTTGGATTGGTTAATGCATTCTGATGTTGTGGAAGGTATATCTCAAGTGGTCAATGCCTCAAATCCAGGAACTGATAGGACTCCAATTGCTATTATAGTTCCAGGGCTCACTAGTGATTCTTCTGCTGCATATATTAAACATATTGCCTTCAGGCTGGCAAAGGAAGGATGGAATGTTGTTGTGCAAAACCACCGTGGACTTGGAGGGATATCTTTAACTTCTGACTGCGTCTACACTGCCGGGTGGACAGAAGATCTTCGGAAAGTAATTGCTCACATCCATTCTCAGTTCCCAGAGGCTCCTTTGTTTGCTGTTGGAACAAGCATTGGTGCCAACGTTCTGGTAAAATATCTTGGGGAGGATGGGCCAAATACACCTCTTATTGGAGCTACTGCTGTGTGTTCTCCTTGGGATCTATTGATATGTGATCGATTTATCAACCGCAAACTCGTGCAAAAAGTGTATGACAGAATGCTTACTATTGGTCTACAAGGTTATGCACAATTGCACCATTCCATCATTTCTCGTATAGCCGATTGGGAAGGCATTAAGAAGTCACGTTCAGTTCGAGAGTTTGACAACTACGCCACCAGGCTTGTTGCCAAATTCGAGACAACTGATACATACTATAGACGTTCAAGCAGTTCACAATATGTGGAAAATGTCGCTGTTCCGCTCCTTTGCATCAGTGCTTTGGATGATCCAGTTTGTACTAGAGAAGCTATTCCTTGGGATGAATGTAGGGCAAACAAAAACATAGTTCTGGCTACCACAACACATGGTGGTCATCTAGCTTACTATGAAGGGTTGACAGCGTCAAGCATGTGGTGGACAAGGGTTGTACACGAATATTTTGAAGTGCTACTCTCCAGCCCCTTTGCAGACAGAAGACACAAGATCATCCCCGAACCATTGGGATCTTCGATTGATCAGGGACCATTCGTGGATGCTGGAGAACACGGGATGGTTGCTGCTGCAAGTGAAGTGGACACAACAAGAGCTGATGCAGAACCAGAGGATAATGCTCAAATTTATGTAAAGACAAGTAATCATCTACACAAGGACAATAATCCTCAAGTTTATAACAATATACTCGGTCCTTTGAAGAAGAGAGTGGATCAGCTTTCAAGATACAGCCGGAAATCGATTTGGCTATTGGCATACATTGCTATAGTCACGACGTGGCCACTAGTAGGACCTGCACTTTTGTTTTCAATTAAGAGAAGATTCAGAAAGCTCGTAGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

537

Amino Acids

60.46

Weight (kDa)

8.0

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 152 - 363 3.1e-12 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1446
Acc16I TGCGCA 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 1570
AccB1I GGYRCC 1 cut(s) 692
AccBSI CCGCTC 1 cut(s) 1039
AccI GTMKAC 2 cut(s) 594, 850
AciI CCGC 2 cut(s) 805, 1037
AclI AACGTT 1 cut(s) 699
AclWI GGATC 5 cut(s) 152, 783, 1058, 1288, 1488
AcoI YGGCCR 1 cut(s) 1547
AcsI RAATTY 3 cut(s) 327, 968, 1392
AcuI CTGAAG 1 cut(s) 496
AdeI CACNNNGTG 2 cut(s) 1145, 1545
AfaI GTAC 2 cut(s) 1075, 1206
AfiI CCNNNNNNNGG 2 cut(s) 746, 1556
AgsI TTSAA 9 cut(s) 55, 178, 261, 275, 1002, 1220, 1468, 1491, 1578
AhlI ACTAGT 2 cut(s) 473, 1552
AjiI CACGTC 1 cut(s) 1545
AjnI CCWGG 3 cut(s) 427, 463, 953
AluBI AGCT 7 cut(s) 281, 752, 1085, 1157, 1364, 1486, 1600
AluI AGCT 7 cut(s) 281, 752, 1085, 1157, 1364, 1486, 1600
Alw26I GTCTC 4 cut(s) 214, 227, 309, 968
AlwI GGATC 5 cut(s) 152, 783, 1058, 1288, 1488
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 2 cut(s) 728, 1547
ApeKI GCWGC 3 cut(s) 488, 1334, 1337
ApoI RAATTY 3 cut(s) 327, 968, 1392
Asp700I GAANNNNTTC 2 cut(s) 618, 1086
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 4 cut(s) 728, 1298, 1460, 1559
AsuC2I CCSGG 1 cut(s) 604
AvaII GGWCC 3 cut(s) 1298, 1460, 1559
BalI TGGCCA 1 cut(s) 1549
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 1 cut(s) 471
BauI CACGAG 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 1258
BbvI GCAGC 3 cut(s) 475, 1321, 1324
BccI CCATC 5 cut(s) 341, 525, 719, 887, 1321
BciT130I CCWGG 3 cut(s) 429, 465, 955
BclI TGATCA 1 cut(s) 1291
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 4 cut(s) 214, 227, 309, 968
BcuI ACTAGT 2 cut(s) 473, 1552
BfaI CTAG 5 cut(s) 134, 474, 1077, 1154, 1553
BfmI CTRYAG 2 cut(s) 991, 1533
BfuAI ACCTGC 1 cut(s) 1570
BglII AGATCT 1 cut(s) 616
BisI GCNGC 3 cut(s) 489, 1335, 1338
BlsI GCNGC 3 cut(s) 490, 1336, 1339
Bme1390I CCNGG 4 cut(s) 429, 465, 604, 955
Bme18I GGWCC 3 cut(s) 1298, 1460, 1559
BmgBI CACGTC 1 cut(s) 1545
BmgT120I GGNCC 4 cut(s) 728, 1298, 1460, 1559
BmiI GGNNCC 3 cut(s) 663, 694, 1299
BmrFI CCNGG 4 cut(s) 429, 465, 604, 955
BmsI GCATC 3 cut(s) 1056, 1300, 1357
BpiI GAAGAC 1 cut(s) 1258
BpmI CTGGAG 2 cut(s) 1216, 1335
BpuEI CTTGAG 2 cut(s) 390, 1422
BpuMI CCSGG 1 cut(s) 604
Bsa29I ATCGAT 2 cut(s) 793, 1508
BsaJI CCNNGG 4 cut(s) 464, 556, 770, 1091
Bsc4I CCNNNNNNNGG 2 cut(s) 746, 1556
Bse1I ACTGG 1 cut(s) 1067
Bse3DI GCAATG 1 cut(s) 1527
BseBI CCWGG 3 cut(s) 429, 465, 955
BseCI ATCGAT 2 cut(s) 793, 1508
BseDI CCNNGG 4 cut(s) 464, 556, 770, 1091
BseLI CCNNNNNNNGG 2 cut(s) 746, 1556
BseMI GCAATG 1 cut(s) 1527
BseMII CTCAG 1 cut(s) 662
BseNI ACTGG 1 cut(s) 1067
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 475, 1321, 1324
BsgI GTGCAG 1 cut(s) 1548
BshFI GGCC 2 cut(s) 730, 1549
BshNI GGYRCC 1 cut(s) 692
BshVI ATCGAT 2 cut(s) 793, 1508
BsiSI CCGG 2 cut(s) 603, 1501
BslFI GGGAC 1 cut(s) 1311
BslI CCNNNNNNNGG 2 cut(s) 746, 1556
BsmAI GTCTC 4 cut(s) 214, 227, 309, 968
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 1311
BsmI GAATGC 2 cut(s) 379, 840
BsnI GGCC 2 cut(s) 730, 1549
Bsp1286I GDGCHC 1 cut(s) 471
Bsp1407I TGTACA 1 cut(s) 1204
BspACI CCGC 2 cut(s) 805, 1037
BspANI GGCC 2 cut(s) 730, 1549
BspCNI CTCAG 1 cut(s) 661
BspDI ATCGAT 2 cut(s) 793, 1508
BspLI GGNNCC 3 cut(s) 663, 694, 1299
BspMI ACCTGC 1 cut(s) 1570
BspPI GGATC 5 cut(s) 152, 783, 1058, 1288, 1488
BspT107I GGYRCC 1 cut(s) 692
BsrBI CCGCTC 1 cut(s) 1039
BsrDI GCAATG 1 cut(s) 1527
BsrGI TGTACA 1 cut(s) 1204
BsrI ACTGG 1 cut(s) 1067
BssECI CCNNGG 4 cut(s) 464, 556, 770, 1091
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 2 cut(s) 770, 1091
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 3 cut(s) 429, 465, 955
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 1466
BstAPI GCANNNNNTGC 1 cut(s) 1529
BstAUI TGTACA 1 cut(s) 1204
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 1 cut(s) 648
BstDSI CCRYGG 1 cut(s) 556
BstHHI GCGC 1 cut(s) 11
BstMAI GTCTC 4 cut(s) 214, 227, 309, 968
BstMWI GCNNNNNNNGC 1 cut(s) 1529
BstNI CCWGG 3 cut(s) 429, 465, 955
BstNSI RCATGY 1 cut(s) 1189
BstSCI CCNGG 4 cut(s) 427, 463, 602, 953
BstSFI CTRYAG 2 cut(s) 991, 1533
BstV1I GCAGC 3 cut(s) 475, 1321, 1324
BstV2I GAAGAC 1 cut(s) 1258
BstX2I RGATCY 4 cut(s) 157, 616, 775, 1280
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 4 cut(s) 157, 616, 775, 1280
Bsu15I ATCGAT 2 cut(s) 793, 1508
BsuRI GGCC 2 cut(s) 730, 1549
BsuTUI ATCGAT 2 cut(s) 793, 1508
BtgI CCRYGG 1 cut(s) 556
BtrI CACGTC 1 cut(s) 1545
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 597, 1057
BveI ACCTGC 1 cut(s) 1570
Cac8I GCNNGC 1 cut(s) 519
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 728, 1298, 1460, 1559
ClaI ATCGAT 2 cut(s) 793, 1508
CseI GACGC 2 cut(s) 580, 1167
Csp6I GTAC 2 cut(s) 1074, 1205
CspCI CAANNNNNGTGG 4 cut(s) 940, 975, 1329, 1364
CviAII CATG 3 cut(s) 230, 1142, 1186
CviQI GTAC 2 cut(s) 1074, 1205
DdeI CTNAG 1 cut(s) 648
DraIII CACNNNGTG 2 cut(s) 1145, 1545
EaeI YGGCCR 1 cut(s) 1547
Eam1104I CTCTTC 1 cut(s) 1466
EarI CTCTTC 1 cut(s) 1466
Eco130I CCWWGG 2 cut(s) 770, 1091
Eco24I GRGCYC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 573
Eco47I GGWCC 3 cut(s) 1298, 1460, 1559
Eco57I CTGAAG 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 1559
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 3 cut(s) 427, 463, 953
EcoRV GATATC 1 cut(s) 573
EcoT14I CCWWGG 2 cut(s) 770, 1091
EcoT22I ATGCAT 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 2 cut(s) 770, 1091
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 3 cut(s) 233, 1145, 1189
FaqI GGGAC 1 cut(s) 1311
FatI CATG 3 cut(s) 229, 1141, 1185
FbaI TGATCA 1 cut(s) 1291
FblI GTMKAC 2 cut(s) 594, 850
Fnu4HI GCNGC 3 cut(s) 489, 1335, 1338
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 3 cut(s) 489, 1335, 1338
FspBI CTAG 5 cut(s) 134, 474, 1077, 1154, 1553
FspI TGCGCA 1 cut(s) 10
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 489, 1335, 1338
GsuI CTGGAG 2 cut(s) 1216, 1335
HaeIII GGCC 2 cut(s) 730, 1549
HapII CCGG 2 cut(s) 603, 1501
HgaI GACGC 2 cut(s) 580, 1167
HhaI GCGC 1 cut(s) 11
Hin1II CATG 3 cut(s) 233, 1145, 1189
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HinfI GANTC 4 cut(s) 139, 442, 479, 1590
HpaII CCGG 2 cut(s) 603, 1501
Hpy166II GTNNAC 9 cut(s) 560, 595, 609, 851, 1010, 1173, 1194, 1207, 1351
Hpy188I TCNGA 5 cut(s) 190, 385, 586, 624, 1595
Hpy188III TCNNGA 4 cut(s) 932, 973, 1491, 1540
Hpy8I GTNNAC 9 cut(s) 560, 595, 609, 851, 1010, 1173, 1194, 1207, 1351
Hpy99I CGWCG 1 cut(s) 1546
HpyAV CCTTC 7 cut(s) 184, 236, 389, 520, 521, 899, 1160
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 5 cut(s) 248, 699, 922, 997, 1544
HpyF10VI GCNNNNNNNGC 1 cut(s) 1529
HpyF3I CTNAG 1 cut(s) 648
HpySE526I ACGT 5 cut(s) 248, 699, 922, 997, 1544
Hsp92II CATG 3 cut(s) 233, 1145, 1189
HspAI GCGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 1291
LmnI GCTCC 3 cut(s) 667, 749, 1044
Lsp1109I GCAGC 3 cut(s) 475, 1321, 1324
LweI GCATC 3 cut(s) 1056, 1300, 1357
MaeI CTAG 5 cut(s) 134, 474, 1077, 1154, 1553
MaeII ACGT 5 cut(s) 248, 699, 922, 997, 1544
MaeIII GTNAC 2 cut(s) 918, 1537
MbiI CCGCTC 1 cut(s) 1039
MfeI CAATTG 3 cut(s) 357, 447, 866
MflI RGATCY 4 cut(s) 157, 616, 775, 1280
MhlI GDGCHC 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 1549
MluCI AATT 9 cut(s) 4, 327, 357, 447, 630, 866, 968, 1392, 1578
MluNI TGGCCA 1 cut(s) 1549
MlyI GAGTC 1 cut(s) 436
MmeI TCCRAC 1 cut(s) 658
Mox20I TGGCCA 1 cut(s) 1549
Mph1103I ATGCAT 1 cut(s) 381
MroXI GAANNNNTTC 2 cut(s) 618, 1086
MscI TGGCCA 1 cut(s) 1549
MseI TTAA 5 cut(s) 374, 498, 578, 912, 1581
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 1549
MspI CCGG 2 cut(s) 603, 1501
MspR9I CCNGG 4 cut(s) 429, 465, 604, 955
MunI CAATTG 3 cut(s) 357, 447, 866
Mva1269I GAATGC 2 cut(s) 379, 840
MvaI CCWGG 3 cut(s) 429, 465, 955
MwoI GCNNNNNNNGC 1 cut(s) 1529
NciI CCSGG 1 cut(s) 604
NlaIII CATG 3 cut(s) 233, 1145, 1189
NlaIV GGNNCC 3 cut(s) 663, 694, 1299
NmuCI GTSAC 2 cut(s) 918, 1537
NsbI TGCGCA 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 381
NspI RCATGY 1 cut(s) 1189
PcsI WCGNNNNNNNCGW 1 cut(s) 928
PctI GAATGC 2 cut(s) 379, 840
PdmI GAANNNNTTC 2 cut(s) 618, 1086
PfeI GAWTC 3 cut(s) 139, 479, 1590
PflFI GACNNNGTC 2 cut(s) 590, 1177
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 3 cut(s) 490, 1336, 1339
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 1559
PsiI TTATAA 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 1559
Psp6I CCWGG 3 cut(s) 427, 463, 953
PspGI CCWGG 3 cut(s) 427, 463, 953
PspN4I GGNNCC 3 cut(s) 663, 694, 1299
PspPI GGNCC 4 cut(s) 728, 1298, 1460, 1559
PspPPI RGGWCCY 1 cut(s) 1559
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 4 cut(s) 157, 616, 775, 1280
PsyI GACNNNGTC 2 cut(s) 590, 1177
RsaI GTAC 2 cut(s) 1075, 1206
RsaNI GTAC 2 cut(s) 1074, 1205
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 5 cut(s) 374, 498, 578, 912, 1581
SatI GCNGC 3 cut(s) 489, 1335, 1338
Sau96I GGNCC 4 cut(s) 728, 1298, 1460, 1559
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 4 cut(s) 429, 465, 604, 955
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 3 cut(s) 1056, 1300, 1357
SfcI CTRYAG 2 cut(s) 991, 1533
SinI GGWCC 3 cut(s) 1298, 1460, 1559
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 405, 1437
SmoI CTYRAG 2 cut(s) 405, 1437
SpeI ACTAGT 2 cut(s) 473, 1552
Sse9I AATT 9 cut(s) 4, 327, 357, 447, 630, 866, 968, 1392, 1578
SsiI CCGC 2 cut(s) 805, 1037
SspI AATATT 1 cut(s) 1214
SspMI CTAG 5 cut(s) 134, 474, 1077, 1154, 1553
StyD4I CCNGG 4 cut(s) 427, 463, 602, 953
StyI CCWWGG 2 cut(s) 770, 1091
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 5 cut(s) 251, 702, 925, 1000, 1547
TaqI TCGA 5 cut(s) 793, 931, 972, 1286, 1508
TaqII GACCGA 1 cut(s) 1448
TasI AATT 9 cut(s) 4, 327, 357, 447, 630, 866, 968, 1392, 1578
TatI WGTACW 2 cut(s) 1073, 1204
TfiI GAWTC 3 cut(s) 139, 479, 1590
Tru1I TTAA 5 cut(s) 374, 498, 578, 912, 1581
Tru9I TTAA 5 cut(s) 374, 498, 578, 912, 1581
TscAI CASTG 2 cut(s) 604, 1057
TseFI GTSAC 2 cut(s) 918, 1537
TseI GCWGC 3 cut(s) 488, 1334, 1337
Tsp45I GTSAC 2 cut(s) 918, 1537
TspDTI ATGAA 5 cut(s) 17, 131, 218, 1113, 1179
TspRI CASTG 2 cut(s) 604, 1057
Tth111I GACNNNGTC 2 cut(s) 590, 1177
VpaK11BI GGWCC 3 cut(s) 1298, 1460, 1559
XapI RAATTY 3 cut(s) 327, 968, 1392
XceI RCATGY 1 cut(s) 1189
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmiI GTMKAC 2 cut(s) 594, 850
XmnI GAANNNNTTC 2 cut(s) 618, 1086
XspI CTAG 5 cut(s) 134, 474, 1077, 1154, 1553
Zsp2I ATGCAT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.