RchiOBHm_Chr6g0272251

Embryogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004956
Physical Location & Seq
Reverse (-)
32435272 .. 32458620
23349 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24424

Sequence Viewer

Length: 1137 bp
ATGTCCCTATTTGTCTCTCTATCGAGCTCAACGCTGCACTCCCCTTCCTTTTCCTCTTCATCTTCCTCTCCCCGTGATAAGAAAAACGAGATGCATAGGATGGTGGATTGGTGGGGTCTCTGTGCAGCTTTAGCGAGCAGCAGCCCCTACCTTGTTTTGCTGAACGATCTCCTTCTCATTCCTATTTGGCACTACGTCTTGGCCTTCCTCACAATCTCCTTCGCATTCCTCTACTATTTCCTTGAATTCCATTTCCTCCATGACCTTTTCACCGGATTCAGAGGCTGCCCTGTCACCTTGACCTACCACTCCTCCTCCCATATCTACCATGACGTCGTTTCCAAGTGTCGGATTCTCCATTCCAGGTATTTGGTCACGCCCTGGCTCTCAAGCCCTCATGTACAGACCGCCTTCCTCAGTATCTTTGGTCGACCTCCACTTTTCAACTACCGGAGACAACTTTTTCATCTATCTGATGGTGGAACTATTGCCTTGGATTGGTTACTGAACTCTGATGTTCTGGGAAATAACTTTGACATAACAACTGCTATTCCGAAAGATGATATAAACCCGATTGTGCTAGCCATTCCTGGATTGACAAGTGACTCCGAATCTGCTAGTATAAAGCATCTTGCTTTTAACACGGCGCAAAAGGGATGGAACGTTGTTGTTATCAACCACAGAGGTTTGGGTGGTGTTCCAATTACTTCCGATTGCTTTTATGATGCTGGTTGGACAGAGGATCTACGGGCAGTCATTAATCATCTCCATCATGAATACCCCAACGCTCCTTTATTTGTTGTTGGAATGAGTATGGGAGCCAACATTTTGGTGAAATATCTTGGGGAGGATGGAGATAGTATTCCAGTTGCTGGAGCTGTAGCAATATGTTCTCCTTGGGACCTTCTGATTAGTGATAGATTTTTACACCGTAGGCTGTTGCAAAAAATTTATGGAAGAGTTCTTGCAATTGGCCTTCAAGACTACGTACAATTGTATTCGCGTTCTATTCGTGATTTTGATCATCATGCTATTCGCCTTCTTGGGAAATTTGAGACTGTGGACACATATTACAGGCATTGTATGGAAATTATTCTATGCACCGACGGTGTAAGTGATCCAACACTTATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

378

Amino Acids

42.62

Weight (kDa)

6.45

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 191 - 332 5.8e-10 alpha/beta hydrolase fold
Hydrolase_4 PF12146 192 - 300 7.4e-13 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
AatII GACGTC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 872
AccI GTMKAC 1 cut(s) 430
AccII CGCG 1 cut(s) 1003
AciI CCGC 1 cut(s) 408
AclI AACGTT 1 cut(s) 663
AclWI GGATC 2 cut(s) 750, 1112
AcsI RAATTY 3 cut(s) 245, 948, 1049
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 2 cut(s) 402, 990
AfiI CCNNNNNNNGG 3 cut(s) 348, 498, 872
AgsI TTSAA 3 cut(s) 245, 445, 980
AjnI CCWGG 3 cut(s) 362, 380, 589
AluBI AGCT 3 cut(s) 27, 128, 878
AluI AGCT 3 cut(s) 27, 128, 878
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 4 cut(s) 19, 122, 448, 1049
AlwI GGATC 2 cut(s) 750, 1112
AlwNI CAGNNNCTG 2 cut(s) 285, 872
AoxI GGCC 2 cut(s) 201, 973
ApeKI GCWGC 5 cut(s) 34, 125, 138, 141, 285
ApoI RAATTY 3 cut(s) 245, 948, 1049
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 1092
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 901
AsuHPI GGTGA 3 cut(s) 262, 286, 844
AsuNHI GCTAGC 1 cut(s) 580
AvaII GGWCC 1 cut(s) 901
BanII GRGCYC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 5 cut(s) 21, 137, 150, 153, 272
BccI CCATC 5 cut(s) 94, 470, 651, 777, 845
BceAI ACGGC 1 cut(s) 660
BciT130I CCWGG 3 cut(s) 364, 382, 591
BclI TGATCA 1 cut(s) 1021
BcoDI GTCTC 4 cut(s) 19, 122, 448, 1049
BfaI CTAG 2 cut(s) 581, 618
BfmI CTRYAG 1 cut(s) 879
BisI GCNGC 5 cut(s) 35, 126, 139, 142, 286
BlsI GCNGC 5 cut(s) 36, 127, 140, 143, 287
Bme1390I CCNGG 3 cut(s) 364, 382, 591
Bme18I GGWCC 1 cut(s) 901
BmgT120I GGNCC 1 cut(s) 901
BmiI GGNNCC 2 cut(s) 820, 902
BmrFI CCNGG 3 cut(s) 364, 382, 591
BmsI GCATC 3 cut(s) 81, 637, 715
BmtI GCTAGC 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 373
BsaAI YACGTR 1 cut(s) 988
BsaBI GATNNNNATC 1 cut(s) 1020
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 380, 492, 896
BsaWI WCCGGW 2 cut(s) 272, 450
BsaXI ACNNNNNCTCC 4 cut(s) 296, 299, 326, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 498, 872
Bse1I ACTGG 1 cut(s) 866
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 3 cut(s) 364, 382, 591
BseDI CCNNGG 3 cut(s) 380, 492, 896
BseGI GGATG 3 cut(s) 105, 662, 856
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 3 cut(s) 348, 498, 872
BseMII CTCAG 1 cut(s) 430
BseNI ACTGG 1 cut(s) 866
BseRI GAGGAG 2 cut(s) 301, 304
BseXI GCAGC 5 cut(s) 21, 137, 150, 153, 272
BsgI GTGCAG 2 cut(s) 20, 144
Bsh1236I CGCG 1 cut(s) 1003
BshFI GGCC 2 cut(s) 203, 975
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 2 cut(s) 273, 451
BslFI GGGAC 1 cut(s) 914
BslI CCNNNNNNNGG 3 cut(s) 348, 498, 872
BsmAI GTCTC 4 cut(s) 19, 122, 448, 1049
BsmFI GGGAC 1 cut(s) 914
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 2 cut(s) 203, 975
Bso31I GGTCTC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 29
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 4 cut(s) 166, 742, 1021, 1117
BspACI CCGC 1 cut(s) 408
BspANI GGCC 2 cut(s) 203, 975
BspCNI CTCAG 1 cut(s) 429
BspFNI CGCG 1 cut(s) 1003
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 2 cut(s) 820, 902
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 2 cut(s) 750, 1112
BspTNI GGTCTC 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 1 cut(s) 866
BssECI CCNNGG 3 cut(s) 380, 492, 896
BssMI GATC 4 cut(s) 166, 742, 1021, 1117
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 492, 896
Bst2UI CCWGG 3 cut(s) 364, 382, 591
Bst4CI ACNGT 3 cut(s) 932, 1060, 1109
Bst6I CTCTTC 2 cut(s) 61, 952
BstACI GRCGYC 1 cut(s) 333
BstAUI TGTACA 1 cut(s) 400
BstBAI YACGTR 1 cut(s) 988
BstC8I GCNNGC 2 cut(s) 136, 582
BstDEI CTNAG 1 cut(s) 416
BstF5I GGATG 3 cut(s) 105, 662, 856
BstFNI CGCG 1 cut(s) 1003
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 4 cut(s) 169, 745, 1024, 1120
BstMAI GTCTC 4 cut(s) 19, 122, 448, 1049
BstMBI GATC 4 cut(s) 166, 742, 1021, 1117
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 3 cut(s) 364, 382, 591
BstSCI CCNGG 3 cut(s) 362, 380, 589
BstSFI CTRYAG 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 988
BstUI CGCG 1 cut(s) 1003
BstV1I GCAGC 5 cut(s) 21, 137, 150, 153, 272
BstX2I RGATCY 1 cut(s) 742
BstXI CCANNNNNNTGG 2 cut(s) 370, 829
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 2 cut(s) 203, 975
BtsCI GGATG 3 cut(s) 105, 662, 856
Cac8I GCNNGC 2 cut(s) 136, 582
CaiI CAGNNNCTG 2 cut(s) 285, 872
CciI TCATGA 1 cut(s) 772
CfoI GCGC 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 901
Csp6I GTAC 2 cut(s) 401, 989
CviAII CATG 5 cut(s) 260, 329, 398, 773, 1028
CviQI GTAC 2 cut(s) 401, 989
DdeI CTNAG 1 cut(s) 416
DpnI GATC 4 cut(s) 168, 744, 1023, 1119
DpnII GATC 4 cut(s) 166, 742, 1021, 1117
Eam1104I CTCTTC 2 cut(s) 61, 952
EarI CTCTTC 2 cut(s) 61, 952
Ecl136II GAGCTC 1 cut(s) 27
Eco105I TACGTA 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 492, 896
Eco24I GRGCYC 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 901
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 901
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 362, 380, 589
EcoT14I CCWWGG 2 cut(s) 492, 896
EcoT22I ATGCAT 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 2 cut(s) 492, 896
FaeI CATG 5 cut(s) 263, 332, 401, 776, 1031
FaqI GGGAC 1 cut(s) 914
FatI CATG 5 cut(s) 259, 328, 397, 772, 1027
FbaI TGATCA 1 cut(s) 1021
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 5 cut(s) 35, 126, 139, 142, 286
FokI GGATG 3 cut(s) 112, 669, 863
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 5 cut(s) 35, 126, 139, 142, 286
FspBI CTAG 2 cut(s) 581, 618
GlaI GCGC 1 cut(s) 648
GluI GCNGC 5 cut(s) 35, 126, 139, 142, 286
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 2 cut(s) 203, 975
HapII CCGG 2 cut(s) 273, 451
HhaI GCGC 1 cut(s) 649
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 5 cut(s) 263, 332, 401, 776, 1031
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 431
HindII GTYRAC 1 cut(s) 431
HinfI GANTC 4 cut(s) 276, 352, 605, 611
HpaII CCGG 2 cut(s) 273, 451
HphI GGTGA 3 cut(s) 262, 286, 844
Hpy166II GTNNAC 2 cut(s) 431, 1063
Hpy188I TCNGA 8 cut(s) 281, 351, 475, 514, 555, 610, 712, 909
Hpy188III TCNNGA 3 cut(s) 773, 980, 1013
Hpy8I GTNNAC 2 cut(s) 431, 1063
Hpy99I CGWCG 2 cut(s) 338, 1109
HpyAV CCTTC 8 cut(s) 54, 182, 214, 229, 421, 914, 986, 1049
HpyCH4III ACNGT 3 cut(s) 932, 1060, 1109
HpyCH4IV ACGT 4 cut(s) 195, 333, 663, 987
HpyCH4V TGCA 6 cut(s) 37, 94, 125, 943, 968, 1101
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 1 cut(s) 416
HpySE526I ACGT 4 cut(s) 195, 333, 663, 987
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 5 cut(s) 263, 332, 401, 776, 1031
HspAI GCGC 1 cut(s) 647
Ksp22I TGATCA 1 cut(s) 1021
Kzo9I GATC 4 cut(s) 166, 742, 1021, 1117
LmnI GCTCC 3 cut(s) 793, 818, 875
Lsp1109I GCAGC 5 cut(s) 21, 137, 150, 153, 272
LweI GCATC 3 cut(s) 81, 637, 715
MaeI CTAG 2 cut(s) 581, 618
MaeII ACGT 4 cut(s) 195, 333, 663, 987
MaeIII GTNAC 4 cut(s) 292, 373, 501, 602
MalI GATC 4 cut(s) 168, 744, 1023, 1119
MboI GATC 4 cut(s) 166, 742, 1021, 1117
MboII GAAGA 3 cut(s) 48, 54, 969
MfeI CAATTG 2 cut(s) 969, 992
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 7 cut(s) 245, 702, 948, 969, 992, 1049, 1089
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 3 cut(s) 329, 713, 784
Mph1103I ATGCAT 1 cut(s) 96
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 2 cut(s) 639, 759
MslI CAYNNNNRTG 1 cut(s) 830
MspI CCGG 2 cut(s) 273, 451
MspR9I CCNGG 3 cut(s) 364, 382, 591
MunI CAATTG 2 cut(s) 969, 992
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 3 cut(s) 364, 382, 591
MvnI CGCG 1 cut(s) 1003
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeII GATC 4 cut(s) 166, 742, 1021, 1117
NheI GCTAGC 1 cut(s) 580
NlaIII CATG 5 cut(s) 263, 332, 401, 776, 1031
NlaIV GGNNCC 2 cut(s) 820, 902
NmuCI GTSAC 3 cut(s) 292, 373, 602
NsiI ATGCAT 1 cut(s) 96
PagI TCATGA 1 cut(s) 772
PctI GAATGC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 3 cut(s) 276, 352, 611
PflMI CCANNNNNTGG 1 cut(s) 872
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 5 cut(s) 36, 127, 140, 143, 287
PleI GAGTC 1 cut(s) 599
PpsI GAGTC 1 cut(s) 599
Ppu21I YACGTR 1 cut(s) 988
PpuMI RGGWCCY 1 cut(s) 901
PshBI ATTAAT 1 cut(s) 759
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 3 cut(s) 362, 380, 589
PspGI CCWGG 3 cut(s) 362, 380, 589
PspN4I GGNNCC 2 cut(s) 820, 902
PspPI GGNCC 1 cut(s) 901
PspPPI RGGWCCY 1 cut(s) 901
PstNI CAGNNNCTG 2 cut(s) 285, 872
PsuI RGATCY 1 cut(s) 742
RsaI GTAC 2 cut(s) 402, 990
RsaNI GTAC 2 cut(s) 401, 989
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 1 cut(s) 29
SalI GTCGAC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 639, 759
SatI GCNGC 5 cut(s) 35, 126, 139, 142, 286
Sau3AI GATC 4 cut(s) 166, 742, 1021, 1117
Sau96I GGNCC 1 cut(s) 901
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 364, 382, 591
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 3 cut(s) 81, 637, 715
SfcI CTRYAG 1 cut(s) 879
SinI GGWCC 1 cut(s) 901
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SnaBI TACGTA 1 cut(s) 988
Sse9I AATT 7 cut(s) 245, 702, 948, 969, 992, 1049, 1089
SsiI CCGC 1 cut(s) 408
SspMI CTAG 2 cut(s) 581, 618
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 362, 380, 589
StyI CCWWGG 2 cut(s) 492, 896
TaaI ACNGT 3 cut(s) 932, 1060, 1109
TaiI ACGT 4 cut(s) 198, 336, 666, 990
TaqI TCGA 2 cut(s) 23, 430
TasI AATT 7 cut(s) 245, 702, 948, 969, 992, 1049, 1089
TatI WGTACW 1 cut(s) 400
TfiI GAWTC 3 cut(s) 276, 352, 611
Tru1I TTAA 2 cut(s) 639, 759
Tru9I TTAA 2 cut(s) 639, 759
TseFI GTSAC 3 cut(s) 292, 373, 602
TseI GCWGC 5 cut(s) 34, 125, 138, 141, 285
Tsp45I GTSAC 3 cut(s) 292, 373, 602
TspDTI ATGAA 3 cut(s) 48, 455, 789
Van91I CCANNNNNTGG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 901
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 3 cut(s) 245, 948, 1049
XmiI GTMKAC 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 2 cut(s) 581, 618
ZraI GACGTC 1 cut(s) 334
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.