MD16G1208100.v1.1

Embryogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004956
Physical Location & Seq
Forward (+)
19534713 .. 19536354
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1208100.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGACTGCTTTCGGAGCTTTCATGACTCTTCCGCCAACCCCTACGAACTTCTTTTCACCGCCGCGACCCTCATTCCCGTCTGGCACTACTTGTTAGGGTTCCTCATCATCTCCTATGTGTTCCTCTACAACTACTTGGACTTCCATTTCTGGCAAGACTTTTTCACTGGATTCAGAGGCTCTTATGTGGACTTGACATACAACTCCAATTCCCACATCTACCACGGCATGGTTTCCAAGTGCCGGGTACTCCATGCCCGGTACTTGGCCACGCCCTGGCTCGCCAGCCCTCATCTGCAGAATCTTTTCCTCAATTTTTTTGGCAACACTCCTGACGTCACATACCAAAGAGAATCTTTTCGCCTGTCGGATGGCGGTACTATTGCGTTGGATTGGGCGGCGGCAGCGAGATCTCATATATTTGGTGGTGGTCTTGTGCCTAAAGATGACAGCACAACCCCCATTGTAGTTGTACTTCCCGGACTAACAAGTGATTCGGATTCCGCTTATGTAAGGCATCTTGCCTTTAACACAGCTAAAATGGGATGGAATGTAGTAATCAGCAACCACAGAGGACTTGGTGGAGTTCCACTTACTTCAGACTTCATCTACAATTGCGGATGGACTGATGATATTCGCGAAGTCAGTAATCACCTCCACCATAAGCATCCCAAGGCTCCATTGTTTCTTATCGGAACAAGCATAGGTGCCAACATTTTGGTGAAGTATCTTGGGGAAGACGGAAACAATGTCCCGGTTGCTGGCGCTGCAGCCATCTGTAATCCGTGGGACTTTCTCATTGGTGACAGATTCATTCAGCGTACCTTGCTGCAGAAGTTATATGACAAAGCTCTAACAAAAGGCCTTCAATGGTATGCACAATTTCACCAGCATGCCTACTCCCGGCTTGCAAATTGGGACGGCAAGATGCATTGCATTCGACACTACCATGCTACCTGTGACGTGGAGAAGGTTGAGACTGTCGACACATTTTACAGGCGCACAAGCAGTTCCATCTACGTGTGCAACGTGGCGGTTCCCCTTCTTTGTATCAGCGCGTTGGACGATCCAATCTGCACCAGAGAGTCCATTCCTTGGGATGAATGTAGGAACAATGTGAATGTTGTTTTGGCCACCACAAAGCACGGCGGTCACTTGGCGTTTTTCGAAGGCTTCACTTGTTCCGGGATCTGGTGGGTGAGTGCTGTGGATGAGTTTTTCAAGGTTCTGAACTGTAGCAAGTATATGCATGTAAAGAAGAATAAGAAGATTAAGAGTACTACTTCTGGGGCAGAATTGTCATTGGACTCTAGTATTCCTAGGGTTGCAGAAGATGGAATCGGAGTGGTGTTTCAATTGGTGAAGGTAGCTGCATTTGTTAAGGGAGTTGCATTCGCCAAAGTTGTTGCCCAAAACTTAGGAATGGGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

478

Amino Acids

53.42

Weight (kDa)

7.64

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 154 - 363 9.2e-11 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 707
AccB7I CCANNNNNTGG 3 cut(s) 229, 276, 1095
AccI GTMKAC 1 cut(s) 984
AccII CGCG 3 cut(s) 65, 639, 1058
AclWI GGATC 2 cut(s) 1061, 1196
AcoI YGGCCR 2 cut(s) 267, 1131
AcuI CTGAAG 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 6 cut(s) 249, 263, 379, 474, 823, 1279
AfiI CCNNNNNNNGG 8 cut(s) 229, 243, 265, 276, 761, 903, 1095, 1191
AflIII ACRYGT 1 cut(s) 1020
AgsI TTSAA 3 cut(s) 869, 1222, 1355
AjiI CACGTC 1 cut(s) 964
AjnI CCWGG 1 cut(s) 275
AjuI GAANNNNNNNTTGG 2 cut(s) 1112, 1144
AluBI AGCT 4 cut(s) 18, 536, 851, 1370
AluI AGCT 4 cut(s) 18, 536, 851, 1370
Alw26I GTCTC 1 cut(s) 971
AlwI GGATC 2 cut(s) 1061, 1196
AoxI GGCC 3 cut(s) 267, 862, 1131
ApeKI GCWGC 5 cut(s) 404, 767, 770, 829, 1370
Asp700I GAANNNNTTC 2 cut(s) 305, 357
AspA2I CCTAGG 1 cut(s) 1319
AspLEI GCGC 3 cut(s) 767, 1002, 1058
AsuC2I CCSGG 6 cut(s) 245, 259, 480, 755, 904, 1186
AsuHPI GGTGA 7 cut(s) 49, 644, 733, 815, 878, 1210, 1372
AsuII TTCGAA 1 cut(s) 1167
AvrII CCTAGG 1 cut(s) 1319
BalI TGGCCA 2 cut(s) 269, 1133
BanI GGYRCC 1 cut(s) 707
BarI GAAGNNNNNNTAC 2 cut(s) 459, 491
BbsI GAAGAC 1 cut(s) 744
BbvI GCAGC 5 cut(s) 416, 754, 782, 816, 1357
BccI CCATC 6 cut(s) 365, 540, 615, 782, 1022, 1328
BceAI ACGGC 3 cut(s) 241, 937, 1162
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 1 cut(s) 277
BcnI CCSGG 6 cut(s) 245, 259, 480, 755, 904, 1186
BcoDI GTCTC 1 cut(s) 971
BfaI CTAG 2 cut(s) 1311, 1320
BfmI CTRYAG 4 cut(s) 296, 768, 830, 1234
BfoI RGCGCY 1 cut(s) 768
BglII AGATCT 1 cut(s) 410
BisI GCNGC 8 cut(s) 63, 399, 402, 405, 768, 771, 830, 1371
BlnI CCTAGG 1 cut(s) 1319
BlsI GCNGC 8 cut(s) 64, 400, 403, 406, 769, 772, 831, 1372
BmcAI AGTACT 1 cut(s) 1279
Bme1390I CCNGG 7 cut(s) 245, 259, 277, 480, 755, 904, 1186
BmgBI CACGTC 1 cut(s) 964
BmiI GGNNCC 4 cut(s) 101, 678, 709, 1038
BmrFI CCNGG 7 cut(s) 245, 259, 277, 480, 755, 904, 1186
BmsI GCATC 3 cut(s) 526, 676, 918
BpiI GAAGAC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 1167
BpuMI CCSGG 6 cut(s) 245, 259, 480, 755, 904, 1186
BsaAI YACGTR 1 cut(s) 1021
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 6 cut(s) 223, 275, 672, 785, 1094, 1319
Bsc4I CCNNNNNNNGG 8 cut(s) 229, 243, 265, 276, 761, 903, 1095, 1191
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 931
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 6 cut(s) 223, 275, 672, 785, 1094, 1319
BseGI GGATG 6 cut(s) 376, 551, 626, 667, 1105, 1216
BseLI CCNNNNNNNGG 8 cut(s) 229, 243, 265, 276, 761, 903, 1095, 1191
BseMI GCAATG 1 cut(s) 931
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 5 cut(s) 416, 754, 782, 816, 1357
BsgI GTGCAG 1 cut(s) 1060
Bsh1236I CGCG 3 cut(s) 65, 639, 1058
BshFI GGCC 3 cut(s) 269, 864, 1133
BshNI GGYRCC 1 cut(s) 707
BsiSI CCGG 6 cut(s) 244, 259, 480, 755, 904, 1185
BslFI GGGAC 3 cut(s) 737, 803, 932
BslI CCNNNNNNNGG 8 cut(s) 229, 243, 265, 276, 761, 903, 1095, 1191
BsmAI GTCTC 1 cut(s) 971
BsmFI GGGAC 3 cut(s) 737, 803, 932
BsmI GAATGC 2 cut(s) 936, 1391
BsnI GGCC 3 cut(s) 269, 864, 1133
Bsp119I TTCGAA 1 cut(s) 1167
Bsp143I GATC 3 cut(s) 410, 1066, 1188
Bsp68I TCGCGA 1 cut(s) 639
BspANI GGCC 3 cut(s) 269, 864, 1133
BspFNI CGCG 3 cut(s) 65, 639, 1058
BspHI TCATGA 1 cut(s) 22
BspLI GGNNCC 4 cut(s) 101, 678, 709, 1038
BspMAI CTGCAG 3 cut(s) 300, 772, 834
BspPI GGATC 2 cut(s) 1061, 1196
BspT104I TTCGAA 1 cut(s) 1167
BspT107I GGYRCC 1 cut(s) 707
BsrDI GCAATG 1 cut(s) 931
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 6 cut(s) 223, 275, 672, 785, 1094, 1319
BssMI GATC 3 cut(s) 410, 1066, 1188
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 3 cut(s) 672, 1094, 1319
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 2 cut(s) 982, 1235
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 1021
BstBI TTCGAA 1 cut(s) 1167
BstC8I GCNNGC 5 cut(s) 282, 286, 763, 894, 909
BstDEI CTNAG 1 cut(s) 1417
BstDSI CCRYGG 2 cut(s) 223, 785
BstF5I GGATG 6 cut(s) 376, 551, 626, 667, 1105, 1216
BstFNI CGCG 3 cut(s) 65, 639, 1058
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 3 cut(s) 767, 1002, 1058
BstKTI GATC 3 cut(s) 413, 1069, 1191
BstMAI GTCTC 1 cut(s) 971
BstMBI GATC 3 cut(s) 410, 1066, 1188
BstMWI GCNNNNNNNGC 4 cut(s) 15, 404, 767, 826
BstNI CCWGG 1 cut(s) 277
BstNSI RCATGY 2 cut(s) 896, 1253
BstSCI CCNGG 7 cut(s) 243, 257, 275, 478, 753, 902, 1184
BstSFI CTRYAG 4 cut(s) 296, 768, 830, 1234
BstUI CGCG 3 cut(s) 65, 639, 1058
BstV1I GCAGC 5 cut(s) 416, 754, 782, 816, 1357
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 2 cut(s) 410, 1188
BstXI CCANNNNNNTGG 1 cut(s) 718
BstYI RGATCY 2 cut(s) 410, 1188
BsuRI GGCC 3 cut(s) 269, 864, 1133
BtgI CCRYGG 2 cut(s) 223, 785
BtrI CACGTC 1 cut(s) 964
BtsCI GGATG 6 cut(s) 376, 551, 626, 667, 1105, 1216
BtsIMutI CAGTG 1 cut(s) 165
BtuMI TCGCGA 1 cut(s) 639
Cac8I GCNNGC 5 cut(s) 282, 286, 763, 894, 909
CciI TCATGA 1 cut(s) 22
CfoI GCGC 3 cut(s) 767, 1002, 1058
Csp6I GTAC 6 cut(s) 248, 262, 378, 473, 822, 1278
CviAII CATG 6 cut(s) 23, 229, 254, 893, 950, 1250
CviQI GTAC 6 cut(s) 248, 262, 378, 473, 822, 1278
DdeI CTNAG 1 cut(s) 1417
DpnI GATC 3 cut(s) 412, 1068, 1190
DpnII GATC 3 cut(s) 410, 1066, 1188
EaeI YGGCCR 2 cut(s) 267, 1131
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 672, 1094, 1319
Eco147I AGGCCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 275
EcoT14I CCWWGG 3 cut(s) 672, 1094, 1319
EcoT22I ATGCAT 2 cut(s) 933, 1251
ErhI CCWWGG 3 cut(s) 672, 1094, 1319
FaeI CATG 6 cut(s) 26, 232, 257, 896, 953, 1253
FalI AAGNNNNNCTT 2 cut(s) 340, 372
FaqI GGGAC 3 cut(s) 737, 803, 932
FatI CATG 6 cut(s) 22, 228, 253, 892, 949, 1249
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 8 cut(s) 63, 399, 402, 405, 768, 771, 830, 1371
FokI GGATG 6 cut(s) 383, 558, 633, 654, 1112, 1223
Fsp4HI GCNGC 8 cut(s) 63, 399, 402, 405, 768, 771, 830, 1371
FspBI CTAG 2 cut(s) 1311, 1320
GlaI GCGC 3 cut(s) 766, 1001, 1057
GluI GCNGC 8 cut(s) 63, 399, 402, 405, 768, 771, 830, 1371
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 3 cut(s) 269, 864, 1133
HapII CCGG 6 cut(s) 244, 259, 480, 755, 904, 1185
HhaI GCGC 3 cut(s) 767, 1002, 1058
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 6 cut(s) 26, 232, 257, 896, 953, 1253
Hin6I GCGC 3 cut(s) 765, 1000, 1056
HinP1I GCGC 3 cut(s) 765, 1000, 1056
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HpaII CCGG 6 cut(s) 244, 259, 480, 755, 904, 1185
HphI GGTGA 7 cut(s) 49, 644, 733, 815, 878, 1210, 1372
Hpy166II GTNNAC 2 cut(s) 190, 985
Hpy188I TCNGA 8 cut(s) 15, 176, 370, 499, 601, 695, 1230, 1343
Hpy188III TCNNGA 3 cut(s) 23, 332, 638
Hpy8I GTNNAC 2 cut(s) 190, 985
HpyAV CCTTC 5 cut(s) 875, 964, 1052, 1163, 1357
HpyCH4III ACNGT 2 cut(s) 982, 1235
HpyCH4IV ACGT 4 cut(s) 336, 963, 1020, 1029
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 404, 767, 826
HpyF3I CTNAG 1 cut(s) 1417
HpySE526I ACGT 4 cut(s) 336, 963, 1020, 1029
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 6 cut(s) 26, 232, 257, 896, 953, 1253
HspAI GCGC 3 cut(s) 765, 1000, 1056
Kzo9I GATC 3 cut(s) 410, 1066, 1188
LmnI GCTCC 2 cut(s) 15, 682
Lsp1109I GCAGC 5 cut(s) 416, 754, 782, 816, 1357
LweI GCATC 3 cut(s) 526, 676, 918
MaeI CTAG 2 cut(s) 1311, 1320
MaeII ACGT 4 cut(s) 336, 963, 1020, 1029
MaeIII GTNAC 4 cut(s) 337, 803, 959, 1151
MalI GATC 3 cut(s) 412, 1068, 1190
MboI GATC 3 cut(s) 410, 1066, 1188
MboII GAAGA 5 cut(s) 21, 749, 1270, 1279, 1343
MfeI CAATTG 2 cut(s) 613, 1355
MflI RGATCY 2 cut(s) 410, 1188
MlsI TGGCCA 2 cut(s) 269, 1133
MluCI AATT 7 cut(s) 208, 313, 613, 881, 913, 1295, 1355
MluNI TGGCCA 2 cut(s) 269, 1133
MlyI GAGTC 3 cut(s) 20, 1094, 1301
MmeI TCCRAC 3 cut(s) 348, 369, 1041
MnlI CCTC 8 cut(s) 80, 113, 134, 170, 300, 320, 566, 665
Mox20I TGGCCA 2 cut(s) 269, 1133
Mph1103I ATGCAT 2 cut(s) 933, 1251
MroXI GAANNNNTTC 2 cut(s) 305, 357
MscI TGGCCA 2 cut(s) 269, 1133
MseI TTAA 4 cut(s) 528, 1272, 1380, 1432
MslI CAYNNNNRTG 4 cut(s) 719, 891, 948, 1019
Msp20I TGGCCA 2 cut(s) 269, 1133
MspI CCGG 6 cut(s) 244, 259, 480, 755, 904, 1185
MspR9I CCNGG 7 cut(s) 245, 259, 277, 480, 755, 904, 1186
MunI CAATTG 2 cut(s) 613, 1355
Mva1269I GAATGC 2 cut(s) 936, 1391
MvaI CCWGG 1 cut(s) 277
MvnI CGCG 3 cut(s) 65, 639, 1058
MwoI GCNNNNNNNGC 4 cut(s) 15, 404, 767, 826
NciI CCSGG 6 cut(s) 245, 259, 480, 755, 904, 1186
NdeII GATC 3 cut(s) 410, 1066, 1188
NlaIII CATG 6 cut(s) 26, 232, 257, 896, 953, 1253
NlaIV GGNNCC 4 cut(s) 101, 678, 709, 1038
NmuCI GTSAC 4 cut(s) 337, 803, 959, 1151
NruI TCGCGA 1 cut(s) 639
NsiI ATGCAT 2 cut(s) 933, 1251
NspI RCATGY 2 cut(s) 896, 1253
NspV TTCGAA 1 cut(s) 1167
PaeI GCATGC 1 cut(s) 896
PagI TCATGA 1 cut(s) 22
PceI AGGCCT 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 1026
PctI GAATGC 2 cut(s) 936, 1391
PdmI GAANNNNTTC 2 cut(s) 305, 357
PfeI GAWTC 7 cut(s) 171, 301, 353, 494, 500, 810, 1338
PflMI CCANNNNNTGG 3 cut(s) 229, 276, 1095
PfoI TCCNGGA 2 cut(s) 478, 1184
PkrI GCNGC 8 cut(s) 64, 400, 403, 406, 769, 772, 831, 1372
PleI GAGTC 3 cut(s) 20, 1093, 1301
PpsI GAGTC 3 cut(s) 20, 1093, 1301
Ppu21I YACGTR 1 cut(s) 1021
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 4 cut(s) 101, 678, 709, 1038
PstI CTGCAG 3 cut(s) 300, 772, 834
PsuI RGATCY 2 cut(s) 410, 1188
RruI TCGCGA 1 cut(s) 639
RsaI GTAC 6 cut(s) 249, 263, 379, 474, 823, 1279
RsaNI GTAC 6 cut(s) 248, 262, 378, 473, 822, 1278
RseI CAYNNNNRTG 4 cut(s) 719, 891, 948, 1019
SalI GTCGAC 1 cut(s) 983
SaqAI TTAA 4 cut(s) 528, 1272, 1380, 1432
SatI GCNGC 8 cut(s) 63, 399, 402, 405, 768, 771, 830, 1371
Sau3AI GATC 3 cut(s) 410, 1066, 1188
ScaI AGTACT 1 cut(s) 1279
SchI GAGTC 3 cut(s) 20, 1094, 1301
ScrFI CCNGG 7 cut(s) 245, 259, 277, 480, 755, 904, 1186
SfaNI GCATC 3 cut(s) 526, 676, 918
SfcI CTRYAG 4 cut(s) 296, 768, 830, 1234
SfuI TTCGAA 1 cut(s) 1167
SmiMI CAYNNNNRTG 4 cut(s) 719, 891, 948, 1019
SphI GCATGC 1 cut(s) 896
Sse9I AATT 7 cut(s) 208, 313, 613, 881, 913, 1295, 1355
SseBI AGGCCT 1 cut(s) 864
SspMI CTAG 2 cut(s) 1311, 1320
StuI AGGCCT 1 cut(s) 864
StyD4I CCNGG 7 cut(s) 243, 257, 275, 478, 753, 902, 1184
StyI CCWWGG 3 cut(s) 672, 1094, 1319
TaaI ACNGT 2 cut(s) 982, 1235
TaiI ACGT 4 cut(s) 339, 966, 1023, 1032
TaqI TCGA 3 cut(s) 940, 984, 1167
TasI AATT 7 cut(s) 208, 313, 613, 881, 913, 1295, 1355
TatI WGTACW 2 cut(s) 472, 1277
TauI GCSGC 3 cut(s) 65, 401, 404
TfiI GAWTC 7 cut(s) 171, 301, 353, 494, 500, 810, 1338
Tru1I TTAA 4 cut(s) 528, 1272, 1380, 1432
Tru9I TTAA 4 cut(s) 528, 1272, 1380, 1432
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 4 cut(s) 337, 803, 959, 1151
TseI GCWGC 5 cut(s) 404, 767, 770, 829, 1370
Tsp45I GTSAC 4 cut(s) 337, 803, 959, 1151
TspDTI ATGAA 4 cut(s) 11, 595, 802, 1116
TspGWI ACGGA 2 cut(s) 756, 774
TspRI CASTG 1 cut(s) 172
Van91I CCANNNNNTGG 3 cut(s) 229, 276, 1095
XceI RCATGY 2 cut(s) 896, 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmaJI CCTAGG 1 cut(s) 1319
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 305, 357
XspI CTAG 2 cut(s) 1311, 1320
ZraI GACGTC 1 cut(s) 337
ZrmI AGTACT 1 cut(s) 1279
Zsp2I ATGCAT 2 cut(s) 933, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.