AT1G45201

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013471
Physical Location & Seq
Forward (+)
17123785 .. 17129223
5439 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G45201.1

Sequence Viewer

Length: 1440 bp
ATGTCCAAAACCAATATGAAATTTTGCAATAGTTATTTTCTGGTCGATCCGACCAAAGCAAGTTTTCTTGATCTCCTTTTGCTTTTGTTTTCCTCCAACCTGACCAGTGCAAGATTCATCGATTCTCCTCCGGATACGCTTAAAGGCTTCCGGAGAAGTTTCGCGAGTCGATGGATCCTTGCGTTGGCCATCTTCCTTCAAAAAGTATTAATGCTCTTAAGTAAACCTTTTGCATTCATTGGTCAAAAGCTCACATATTGGCTCAACCTTCTTACGGCAAATGGAGGCTTCTTCAACTTGATACTTAACCTTATGTCAGGAAAGCTGGTCAAGCCTGACAAATCTTCGGCGACGTATACTTCTTTTATAGGATGCTCAGATCGACGAATAGAGCTTGACGAGAAGATAAATGTTGGTAGCATCGAATACAAATCGATGTTGTCAATAATGGCTTCTAAGATCTCTTATGAGAGCAAACCTTACATCACTTCCGTCGTCAAAAACACTTGGAAGATGGACTTGGTGGGTAACTACGACTTTTACAACGCTTTCCAAGAAAGTAAATTGACGCAAGCCTTCGTGTTCAAGACTTCGAGCACCAATCCAGACCTCATCGTCGTTAGCTTCAGAGGAACCGAACCTTTCGAGGCTGCCGATTGGTGCACTGATCTCGACCTCTCTTGGTACGAGATGAAGAACGTTGGCAAAGTCCACGCCGGGTTCTCAAGAGCTTTAGGTCTCCAAAAAGATGGATGGCCCAAGGAAAATATTAGTCTACTACACCAATATGCTTACTACACCATCAGACAGATGCTTAGAGACAAGCTTGGCAGAAACAAGAACCTTAAATATATTCTAACAGGTCACAGCCTTGGCGGAGCACTAGCTGCTCTTTTCCCGGCGATTCTGGCCATTCACGGTGAGGATGAGCTGCTAGATAAGCTAGAGGGAATCTATACGTTTGGACAGCCACGTGTAGGAGATGAGGACTTTGGTGAGTTTATGAAGGGTGTGGTGAAAAAGCATGGAATAGAGTATGAGAGATTCGTCTATAACAATGATGTTGTGCCTAGAGTGCCCTTTGATGACAAGTACTTATTCTCATACAAGCACTATGGACCTTGCAATTCCTTCAACAGTCTCTACAAAGGAAAGGTAAGAGAAGATGCACCAAATGCGAACTACTTCAACTTGTTGTGGTTAATACCGCAGCTGTTGACTGGTTTGTGGGAGTTTATAAGGAGTTTCATATTACAGTTTTGGAAAGGCGACGAGTACAAAGAGAATTGGTTAATGAGATTTGTTAGGGTTGTGGGAATAGTGTTCCCTGGTGGCTCTAACCATTTCCCATTTGATTACGTCAACTCTACTCGCCTAGGAGGCTTGGTTCGACCTCCTCCTACTACTACTCCTGAGGATAAACTTGCCCTCATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

479

Amino Acids

54.78

Weight (kDa)

9.04

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 206 - 362 6.5e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1238
AasI GACNNNNNNGTC 1 cut(s) 614
AccI GTMKAC 2 cut(s) 356, 775
AccII CGCG 1 cut(s) 164
AccIII TCCGGA 2 cut(s) 130, 150
AciI CCGC 2 cut(s) 876, 1208
AclI AACGTT 1 cut(s) 699
AclWI GGATC 3 cut(s) 41, 169, 182
AcoI YGGCCR 2 cut(s) 186, 909
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 610
AcvI CACGTG 1 cut(s) 974
AfaI GTAC 3 cut(s) 686, 1094, 1277
AfiI CCNNNNNNNGG 3 cut(s) 184, 274, 977
AflII CTTAAG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 973
AgsI TTSAA 5 cut(s) 200, 295, 586, 1135, 1189
AjnI CCWGG 1 cut(s) 1327
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AloI GAACNNNNNNTCC 2 cut(s) 1371, 1403
Alw21I GWGCWC 3 cut(s) 599, 665, 883
Alw26I GTCTC 3 cut(s) 743, 813, 1145
Alw44I GTGCAC 1 cut(s) 661
AlwI GGATC 3 cut(s) 41, 169, 182
Aor13HI TCCGGA 2 cut(s) 130, 150
AoxI GGCC 3 cut(s) 186, 755, 909
ApaLI GTGCAC 1 cut(s) 661
ApeKI GCWGC 4 cut(s) 650, 887, 931, 1210
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 1184
AspA2I CCTAGG 1 cut(s) 1375
AspS9I GGNCC 2 cut(s) 756, 1118
AsuC2I CCSGG 2 cut(s) 718, 899
AsuHPI GGTGA 3 cut(s) 932, 1007, 1027
AvaII GGWCC 1 cut(s) 1118
AvrII CCTAGG 1 cut(s) 1375
AxyI CCTNAGG 1 cut(s) 1413
BaeGI GKGCMC 2 cut(s) 665, 1080
BalI TGGCCA 2 cut(s) 188, 911
BamHI GGATCC 1 cut(s) 174
BbrPI CACGTG 1 cut(s) 974
Bbv12I GWGCWC 3 cut(s) 599, 665, 883
BbvI GCAGC 4 cut(s) 637, 874, 918, 1222
BccI CCATC 6 cut(s) 165, 197, 508, 743, 747, 809
BceAI ACGGC 1 cut(s) 291
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 1 cut(s) 1329
BciVI GTATCC 1 cut(s) 127
BcnI CCSGG 2 cut(s) 718, 899
BcoDI GTCTC 3 cut(s) 743, 813, 1145
BfaI CTAG 5 cut(s) 884, 935, 944, 1071, 1376
BfrI CTTAAG 1 cut(s) 217
BfuI GTATCC 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 1380
BglII AGATCT 1 cut(s) 459
BisI GCNGC 4 cut(s) 651, 888, 932, 1211
BlnI CCTAGG 1 cut(s) 1375
BlsI GCNGC 4 cut(s) 652, 889, 933, 1212
BmcAI AGTACT 1 cut(s) 1094
Bme1390I CCNGG 3 cut(s) 718, 899, 1329
Bme18I GGWCC 1 cut(s) 1118
BmgT120I GGNCC 2 cut(s) 756, 1118
BmiI GGNNCC 2 cut(s) 176, 634
BmrFI CCNGG 3 cut(s) 718, 899, 1329
BmsI GCATC 4 cut(s) 362, 429, 801, 1156
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 2 cut(s) 718, 899
Bsa29I ATCGAT 2 cut(s) 120, 434
BsaAI YACGTR 1 cut(s) 974
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 4 cut(s) 759, 871, 1327, 1375
BsaWI WCCGGW 2 cut(s) 130, 150
BsaXI ACNNNNNCTCC 4 cut(s) 1371, 1393, 1401, 1423
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 274, 977
Bse1I ACTGG 2 cut(s) 105, 1225
Bse21I CCTNAGG 1 cut(s) 1413
Bse3DI GCAATG 1 cut(s) 1431
BseAI TCCGGA 2 cut(s) 130, 150
BseBI CCWGG 1 cut(s) 1329
BseCI ATCGAT 2 cut(s) 120, 434
BseDI CCNNGG 4 cut(s) 759, 871, 1327, 1375
BseGI GGATG 3 cut(s) 377, 758, 931
BseLI CCNNNNNNNGG 3 cut(s) 184, 274, 977
BseMI GCAATG 1 cut(s) 1431
BseMII CTCAG 2 cut(s) 390, 1404
BseNI ACTGG 2 cut(s) 105, 1225
BseRI GAGGAG 2 cut(s) 117, 1386
BseSI GKGCMC 2 cut(s) 665, 1080
BseXI GCAGC 4 cut(s) 637, 874, 918, 1222
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 3 cut(s) 188, 757, 911
BshVI ATCGAT 2 cut(s) 120, 434
BsiHKAI GWGCWC 3 cut(s) 599, 665, 883
BsiSI CCGG 4 cut(s) 131, 151, 717, 899
BslI CCNNNNNNNGG 3 cut(s) 184, 274, 977
BsmAI GTCTC 3 cut(s) 743, 813, 1145
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 3 cut(s) 188, 757, 911
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 4 cut(s) 599, 665, 883, 1080
Bsp13I TCCGGA 2 cut(s) 130, 150
Bsp143I GATC 6 cut(s) 46, 70, 174, 379, 459, 667
Bsp68I TCGCGA 1 cut(s) 164
BspACI CCGC 2 cut(s) 876, 1208
BspANI GGCC 3 cut(s) 188, 757, 911
BspCNI CTCAG 2 cut(s) 389, 1405
BspDI ATCGAT 2 cut(s) 120, 434
BspEI TCCGGA 2 cut(s) 130, 150
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 2 cut(s) 176, 634
BspPI GGATC 3 cut(s) 41, 169, 182
BspTI CTTAAG 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 1431
BsrI ACTGG 2 cut(s) 105, 1225
BssECI CCNNGG 4 cut(s) 759, 871, 1327, 1375
BssMI GATC 6 cut(s) 46, 70, 174, 379, 459, 667
BssNAI GTATAC 1 cut(s) 357
BssT1I CCWWGG 3 cut(s) 759, 871, 1375
Bst1107I GTATAC 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 1329
Bst4CI ACNGT 3 cut(s) 920, 1139, 1257
BstAFI CTTAAG 1 cut(s) 217
BstAPI GCANNNNNTGC 2 cut(s) 887, 1175
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 1 cut(s) 573
BstDEI CTNAG 4 cut(s) 376, 456, 815, 1413
BstF5I GGATG 3 cut(s) 377, 758, 931
BstFNI CGCG 1 cut(s) 164
BstKTI GATC 6 cut(s) 49, 73, 177, 382, 462, 670
BstMAI GTCTC 3 cut(s) 743, 813, 1145
BstMBI GATC 6 cut(s) 46, 70, 174, 379, 459, 667
BstMWI GCNNNNNNNGC 8 cut(s) 331, 887, 908, 940, 1075, 1175, 1380, 1433
BstNI CCWGG 1 cut(s) 1329
BstSCI CCNGG 3 cut(s) 716, 897, 1327
BstSLI GKGCMC 2 cut(s) 665, 1080
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 4 cut(s) 637, 874, 918, 1222
BstX2I RGATCY 2 cut(s) 174, 459
BstXI CCANNNNNNTGG 1 cut(s) 749
BstYI RGATCY 2 cut(s) 174, 459
BstZ17I GTATAC 1 cut(s) 357
Bsu15I ATCGAT 2 cut(s) 120, 434
Bsu36I CCTNAGG 1 cut(s) 1413
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 3 cut(s) 188, 757, 911
BsuTUI ATCGAT 2 cut(s) 120, 434
BtsCI GGATG 3 cut(s) 377, 758, 931
BtsIMutI CAGTG 2 cut(s) 112, 663
BtuMI TCGCGA 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 573
Cfr13I GGNCC 2 cut(s) 756, 1118
ClaI ATCGAT 2 cut(s) 120, 434
CseI GACGC 1 cut(s) 577
Csp6I GTAC 3 cut(s) 685, 1093, 1276
CviAII CATG 1 cut(s) 1025
CviQI GTAC 3 cut(s) 685, 1093, 1276
DdeI CTNAG 4 cut(s) 376, 456, 815, 1413
DpnI GATC 6 cut(s) 48, 72, 176, 381, 461, 669
DpnII GATC 6 cut(s) 46, 70, 174, 379, 459, 667
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 2 cut(s) 186, 909
EciI GGCGGA 1 cut(s) 891
Eco130I CCWWGG 3 cut(s) 759, 871, 1375
Eco31I GGTCTC 1 cut(s) 743
Eco47I GGWCC 1 cut(s) 1118
Eco57I CTGAAG 1 cut(s) 610
Eco72I CACGTG 1 cut(s) 974
Eco81I CCTNAGG 1 cut(s) 1413
EcoRII CCWGG 1 cut(s) 1327
EcoT14I CCWWGG 3 cut(s) 759, 871, 1375
ErhI CCWWGG 3 cut(s) 759, 871, 1375
FaeI CATG 1 cut(s) 1028
FalI AAGNNNNNCTT 4 cut(s) 211, 243, 503, 535
FatI CATG 1 cut(s) 1024
FblI GTMKAC 2 cut(s) 356, 775
Fnu4HI GCNGC 4 cut(s) 651, 888, 932, 1211
FokI GGATG 3 cut(s) 384, 765, 938
Fsp4HI GCNGC 4 cut(s) 651, 888, 932, 1211
FspBI CTAG 5 cut(s) 884, 935, 944, 1071, 1376
GluI GCNGC 4 cut(s) 651, 888, 932, 1211
HaeIII GGCC 3 cut(s) 188, 757, 911
HapII CCGG 4 cut(s) 131, 151, 717, 899
HgaI GACGC 1 cut(s) 577
Hin1II CATG 1 cut(s) 1028
HincII GTYRAC 2 cut(s) 1218, 1363
HindII GTYRAC 2 cut(s) 1218, 1363
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 6 cut(s) 114, 122, 166, 904, 951, 1044
HpaII CCGG 4 cut(s) 131, 151, 717, 899
HphI GGTGA 3 cut(s) 932, 1007, 1027
Hpy166II GTNNAC 7 cut(s) 224, 357, 663, 712, 776, 1218, 1363
Hpy188I TCNGA 4 cut(s) 51, 379, 629, 806
Hpy8I GTNNAC 7 cut(s) 224, 357, 663, 712, 776, 1218, 1363
Hpy99I CGWCG 5 cut(s) 355, 387, 497, 620, 1274
HpyAV CCTTC 5 cut(s) 206, 278, 586, 1000, 1141
HpyCH4III ACNGT 3 cut(s) 920, 1139, 1257
HpyCH4IV ACGT 5 cut(s) 353, 699, 959, 973, 1359
HpyCH4V TGCA 6 cut(s) 27, 110, 233, 663, 1125, 1169
HpyF10VI GCNNNNNNNGC 8 cut(s) 331, 887, 908, 940, 1075, 1175, 1380, 1433
HpyF3I CTNAG 4 cut(s) 376, 456, 815, 1413
HpySE526I ACGT 5 cut(s) 353, 699, 959, 973, 1359
Hsp92II CATG 1 cut(s) 1028
Kpn2I TCCGGA 2 cut(s) 130, 150
Kzo9I GATC 6 cut(s) 46, 70, 174, 379, 459, 667
LmnI GCTCC 1 cut(s) 878
Lsp1109I GCAGC 4 cut(s) 637, 874, 918, 1222
LweI GCATC 4 cut(s) 362, 429, 801, 1156
MaeI CTAG 5 cut(s) 884, 935, 944, 1071, 1376
MaeII ACGT 5 cut(s) 353, 699, 959, 973, 1359
MaeIII GTNAC 2 cut(s) 527, 863
MalI GATC 6 cut(s) 48, 72, 176, 381, 461, 669
MboI GATC 6 cut(s) 46, 70, 174, 379, 459, 667
MboII GAAGA 7 cut(s) 184, 283, 336, 415, 523, 706, 1175
MflI RGATCY 2 cut(s) 174, 459
MhlI GDGCHC 4 cut(s) 599, 665, 883, 1080
MlsI TGGCCA 2 cut(s) 188, 911
MluCI AATT 4 cut(s) 20, 563, 1126, 1285
MluNI TGGCCA 2 cut(s) 188, 911
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 2 cut(s) 74, 120
Mox20I TGGCCA 2 cut(s) 188, 911
MroI TCCGGA 2 cut(s) 130, 150
MroXI GAANNNNTTC 1 cut(s) 1184
MscI TGGCCA 2 cut(s) 188, 911
MseI TTAA 7 cut(s) 141, 209, 218, 306, 846, 1202, 1292
MslI CAYNNNNRTG 1 cut(s) 786
Msp20I TGGCCA 2 cut(s) 188, 911
MspA1I CMGCKG 1 cut(s) 1213
MspCI CTTAAG 1 cut(s) 217
MspI CCGG 4 cut(s) 131, 151, 717, 899
MspR9I CCNGG 3 cut(s) 718, 899, 1329
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 1 cut(s) 1329
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 8 cut(s) 331, 887, 908, 940, 1075, 1175, 1380, 1433
NciI CCSGG 2 cut(s) 718, 899
NdeII GATC 6 cut(s) 46, 70, 174, 379, 459, 667
NlaIII CATG 1 cut(s) 1028
NlaIV GGNNCC 2 cut(s) 176, 634
NmuCI GTSAC 1 cut(s) 863
NruI TCGCGA 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PctI GAATGC 1 cut(s) 233
PdmI GAANNNNTTC 1 cut(s) 1184
PfeI GAWTC 5 cut(s) 114, 122, 904, 951, 1044
PkrI GCNGC 4 cut(s) 652, 889, 933, 1212
PleI GAGTC 1 cut(s) 174
PmaCI CACGTG 1 cut(s) 974
PmlI CACGTG 1 cut(s) 974
PpsI GAGTC 1 cut(s) 174
Ppu21I YACGTR 1 cut(s) 974
PshBI ATTAAT 1 cut(s) 209
PsiI TTATAA 1 cut(s) 1238
Psp1406I AACGTT 1 cut(s) 699
Psp6I CCWGG 1 cut(s) 1327
PspCI CACGTG 1 cut(s) 974
PspGI CCWGG 1 cut(s) 1327
PspN4I GGNNCC 2 cut(s) 176, 634
PspPI GGNCC 2 cut(s) 756, 1118
PsuI RGATCY 2 cut(s) 174, 459
PvuII CAGCTG 1 cut(s) 1213
RruI TCGCGA 1 cut(s) 164
RsaI GTAC 3 cut(s) 686, 1094, 1277
RsaNI GTAC 3 cut(s) 685, 1093, 1276
RseI CAYNNNNRTG 1 cut(s) 786
SaqAI TTAA 7 cut(s) 141, 209, 218, 306, 846, 1202, 1292
SatI GCNGC 4 cut(s) 651, 888, 932, 1211
Sau3AI GATC 6 cut(s) 46, 70, 174, 379, 459, 667
Sau96I GGNCC 2 cut(s) 756, 1118
ScaI AGTACT 1 cut(s) 1094
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 3 cut(s) 718, 899, 1329
SduI GDGCHC 4 cut(s) 599, 665, 883, 1080
SfaNI GCATC 4 cut(s) 362, 429, 801, 1156
SinI GGWCC 1 cut(s) 1118
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 2 cut(s) 217, 724
SmoI CTYRAG 2 cut(s) 217, 724
Sse9I AATT 4 cut(s) 20, 563, 1126, 1285
SsiI CCGC 2 cut(s) 876, 1208
SspI AATATT 1 cut(s) 769
SspMI CTAG 5 cut(s) 884, 935, 944, 1071, 1376
StyD4I CCNGG 3 cut(s) 716, 897, 1327
StyI CCWWGG 3 cut(s) 759, 871, 1375
TaaI ACNGT 3 cut(s) 920, 1139, 1257
TaiI ACGT 5 cut(s) 356, 702, 962, 976, 1362
TasI AATT 4 cut(s) 20, 563, 1126, 1285
TatI WGTACW 2 cut(s) 1092, 1275
TfiI GAWTC 5 cut(s) 114, 122, 904, 951, 1044
Tru1I TTAA 7 cut(s) 141, 209, 218, 306, 846, 1202, 1292
Tru9I TTAA 7 cut(s) 141, 209, 218, 306, 846, 1202, 1292
TscAI CASTG 2 cut(s) 112, 670
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 4 cut(s) 650, 887, 931, 1210
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 6 cut(s) 32, 106, 226, 707, 1019, 1237
TspGWI ACGGA 1 cut(s) 481
TspRI CASTG 2 cut(s) 112, 670
Vha464I CTTAAG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 661
VpaK11BI GGWCC 1 cut(s) 1118
VspI ATTAAT 1 cut(s) 209
XapI RAATTY 1 cut(s) 20
XmaJI CCTAGG 1 cut(s) 1375
XmiI GTMKAC 2 cut(s) 356, 775
XmnI GAANNNNTTC 1 cut(s) 1184
XspI CTAG 5 cut(s) 884, 935, 944, 1071, 1376
ZrmI AGTACT 1 cut(s) 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.