Rh2CG185500

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013471
Physical Location & Seq
Reverse (-)
17231908 .. 17234445
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG185500.1

Sequence Viewer

Length: 1056 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGGCTGGCTACATAGTGGAGGGAGAAGATGCTATGATATTGTGCCCAACGGGAGAGGAGGAAAATCTCAAACGTAAATGGCTTATATCTTTCATCCTCCTGCTTCAAATGATTCTGTTATGGATTGCCAAACCCATGAGAAATTTTGGTTTAGTTTTTGAGTGGTTCCTCAACCTTCCTTCTAGTAAGCGAAACTGGCTTGAGCTCTCTCTTGATCTTTTAACAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTTAGATGTCAGATATAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCATTGGAAGATGAAACTAATCAAGTTCTTTGACTTTTATAATTATTATCAAGGAAGACATACTACTCAAGCCTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCGAAGGTTTTCCTGACAAAGAACCAGAACTAGATGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACATGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

351

Amino Acids

40.62

Weight (kDa)

8.74

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 201 - 351 1.6e-30 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
Acc65I GGTACC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 617
AccB7I CCANNNNNTGG 2 cut(s) 862, 968
AclWI GGATC 1 cut(s) 1043
AcoI YGGCCR 1 cut(s) 853
AcsI RAATTY 3 cut(s) 17, 229, 839
AfaI GTAC 5 cut(s) 78, 619, 649, 671, 773
AfiI CCNNNNNNNGG 2 cut(s) 862, 968
AgsI TTSAA 5 cut(s) 22, 46, 194, 479, 986
AjnI CCWGG 1 cut(s) 665
AluBI AGCT 4 cut(s) 73, 292, 782, 861
AluI AGCT 4 cut(s) 73, 292, 782, 861
Alw21I GWGCWC 3 cut(s) 294, 430, 874
Alw44I GTGCAC 1 cut(s) 870
AlwI GGATC 1 cut(s) 1043
AoxI GGCC 1 cut(s) 853
ApaLI GTGCAC 1 cut(s) 870
ApeKI GCWGC 3 cut(s) 443, 890, 958
ApoI RAATTY 3 cut(s) 17, 229, 839
Asp700I GAANNNNTTC 1 cut(s) 738
Asp718I GGTACC 1 cut(s) 617
AspA2I CCTAGG 1 cut(s) 718
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 62
AvrII CCTAGG 1 cut(s) 718
BaeGI GKGCMC 2 cut(s) 134, 874
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 1 cut(s) 294
BarI GAAGNNNNNNTAC 2 cut(s) 541, 573
BauI CACGAG 2 cut(s) 815, 963
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 3 cut(s) 294, 430, 874
BbvI GCAGC 3 cut(s) 430, 902, 970
BciT130I CCWGG 1 cut(s) 667
BclI TGATCA 1 cut(s) 589
BfaI CTAG 3 cut(s) 270, 719, 761
BfmI CTRYAG 1 cut(s) 676
BglII AGATCT 1 cut(s) 811
BisI GCNGC 3 cut(s) 444, 891, 959
BlnI CCTAGG 1 cut(s) 718
BlsI GCNGC 3 cut(s) 445, 892, 960
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 1 cut(s) 667
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 2 cut(s) 254, 619
BmrFI CCNGG 1 cut(s) 667
BmsI GCATC 5 cut(s) 106, 405, 622, 628, 754
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 2 cut(s) 308, 546
BsaJI CCNNGG 1 cut(s) 718
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 968
Bse118I RCCGGY 2 cut(s) 402, 851
Bse1I ACTGG 2 cut(s) 287, 879
Bse3DI GCAATG 2 cut(s) 444, 902
BseBI CCWGG 1 cut(s) 667
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 862, 968
BseMI GCAATG 2 cut(s) 444, 902
BseMII CTCAG 2 cut(s) 351, 808
BseNI ACTGG 2 cut(s) 287, 879
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 874
BseXI GCAGC 3 cut(s) 430, 902, 970
BshFI GGCC 1 cut(s) 855
BshNI GGYRCC 1 cut(s) 617
BsiHKAI GWGCWC 3 cut(s) 294, 430, 874
BsiSI CCGG 2 cut(s) 403, 852
BslI CCNNNNNNNGG 2 cut(s) 862, 968
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 855
Bsp1286I GDGCHC 4 cut(s) 134, 294, 430, 874
Bsp143I GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BspANI GGCC 1 cut(s) 855
BspCNI CTCAG 2 cut(s) 350, 807
BspLI GGNNCC 2 cut(s) 254, 619
BspPI GGATC 1 cut(s) 1043
BspQI GCTCTTC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 444, 902
BsrFI RCCGGY 2 cut(s) 402, 851
BsrI ACTGG 2 cut(s) 287, 879
BssAI RCCGGY 2 cut(s) 402, 851
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BssSI CACGAG 2 cut(s) 815, 963
BssT1I CCWWGG 1 cut(s) 718
Bst2BI CACGAG 2 cut(s) 815, 963
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 2 cut(s) 358, 487
Bst6I CTCTTC 1 cut(s) 418
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 337, 794
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 7 cut(s) 69, 304, 592, 600, 814, 1023, 1038
BstMBI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BstMWI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 899
BstNI CCWGG 1 cut(s) 667
BstSCI CCNGG 1 cut(s) 665
BstSFI CTRYAG 1 cut(s) 676
BstSLI GKGCMC 2 cut(s) 134, 874
BstV1I GCAGC 3 cut(s) 430, 902, 970
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 1 cut(s) 855
BtsCI GGATG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 354, 872
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 2 cut(s) 402, 851
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 5 cut(s) 77, 618, 648, 670, 772
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 7 cut(s) 223, 571, 578, 642, 698, 835, 994
CviQI GTAC 5 cut(s) 77, 618, 648, 670, 772
DdeI CTNAG 2 cut(s) 337, 794
DpnI GATC 7 cut(s) 68, 303, 591, 599, 813, 1022, 1037
DpnII GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
EaeI YGGCCR 1 cut(s) 853
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 718
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 292
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 665
EcoT14I CCWWGG 1 cut(s) 718
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 7 cut(s) 226, 574, 581, 645, 701, 838, 997
FatI CATG 7 cut(s) 222, 570, 577, 641, 697, 834, 993
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 3 cut(s) 444, 891, 959
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 444, 891, 959
FspBI CTAG 3 cut(s) 270, 719, 761
GluI GCNGC 3 cut(s) 444, 891, 959
HaeIII GGCC 1 cut(s) 855
HapII CCGG 2 cut(s) 403, 852
Hin1II CATG 7 cut(s) 226, 574, 581, 645, 701, 838, 997
HinfI GANTC 4 cut(s) 199, 335, 799, 990
HpaII CCGG 2 cut(s) 403, 852
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 872
Hpy188I TCNGA 4 cut(s) 66, 416, 614, 1032
Hpy188III TCNNGA 4 cut(s) 299, 743, 796, 817
Hpy8I GTNNAC 1 cut(s) 872
HpyAV CCTTC 5 cut(s) 272, 276, 481, 577, 728
HpyCH4III ACNGT 2 cut(s) 358, 487
HpyCH4IV ACGT 2 cut(s) 160, 950
HpyCH4V TGCA 8 cut(s) 437, 635, 641, 684, 872, 890, 907, 937
HpyF10VI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 899
HpyF3I CTNAG 2 cut(s) 337, 794
HpySE526I ACGT 2 cut(s) 160, 950
Hsp92II CATG 7 cut(s) 226, 574, 581, 645, 701, 838, 997
KpnI GGTACC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
LguI GCTCTTC 1 cut(s) 418
LmnI GCTCC 2 cut(s) 579, 772
Lsp1109I GCAGC 3 cut(s) 430, 902, 970
LweI GCATC 5 cut(s) 106, 405, 622, 628, 754
MaeI CTAG 3 cut(s) 270, 719, 761
MaeII ACGT 2 cut(s) 160, 950
MaeIII GTNAC 3 cut(s) 314, 847, 1046
MalI GATC 7 cut(s) 68, 303, 591, 599, 813, 1022, 1037
MboI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
MboII GAAGA 5 cut(s) 125, 435, 514, 561, 926
MfeI CAATTG 2 cut(s) 593, 803
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 4 cut(s) 134, 294, 430, 874
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 3 cut(s) 340, 366, 906
MnlI CCTC 8 cut(s) 100, 136, 139, 194, 266, 608, 665, 1026
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 2 cut(s) 308, 725
MspI CCGG 2 cut(s) 403, 852
MspR9I CCNGG 1 cut(s) 667
MunI CAATTG 2 cut(s) 593, 803
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 1 cut(s) 667
MwoI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 899
NdeI CATATG 1 cut(s) 457
NdeII GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
NlaIII CATG 7 cut(s) 226, 574, 581, 645, 701, 838, 997
NlaIV GGNNCC 2 cut(s) 254, 619
NmuCI GTSAC 2 cut(s) 314, 847
NsiI ATGCAT 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 418
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 199, 799, 990
PflMI CCANNNNNTGG 2 cut(s) 862, 968
PkrI GCNGC 3 cut(s) 445, 892, 960
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 2 cut(s) 254, 619
PspPI GGNCC 1 cut(s) 62
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 5 cut(s) 78, 619, 649, 671, 773
RsaNI GTAC 5 cut(s) 77, 618, 648, 670, 772
SacI GAGCTC 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 418
SaqAI TTAA 2 cut(s) 308, 725
SatI GCNGC 3 cut(s) 444, 891, 959
Sau3AI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
Sau96I GGNCC 1 cut(s) 62
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 667
SduI GDGCHC 4 cut(s) 134, 294, 430, 874
SfaNI GCATC 5 cut(s) 106, 405, 622, 628, 754
SfcI CTRYAG 1 cut(s) 676
SinI GGWCC 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 287, 561
SmoI CTYRAG 2 cut(s) 287, 561
SspMI CTAG 3 cut(s) 270, 719, 761
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 1 cut(s) 665
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 2 cut(s) 358, 487
TaiI ACGT 2 cut(s) 163, 953
TaqI TCGA 2 cut(s) 15, 657
TatI WGTACW 1 cut(s) 647
TfiI GAWTC 3 cut(s) 199, 799, 990
Tru1I TTAA 2 cut(s) 308, 725
Tru9I TTAA 2 cut(s) 308, 725
TscAI CASTG 2 cut(s) 361, 879
TseFI GTSAC 2 cut(s) 314, 847
TseI GCWGC 3 cut(s) 443, 890, 958
Tsp45I GTSAC 2 cut(s) 314, 847
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 2 cut(s) 361, 879
Van91I CCANNNNNTGG 2 cut(s) 862, 968
VneI GTGCAC 1 cut(s) 870
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 3 cut(s) 17, 229, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XmaJI CCTAGG 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 3 cut(s) 270, 719, 761
ZrmI AGTACT 1 cut(s) 649
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.