Rmu_sc0021235.1_g000003

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021235.1
OG0013471
Physical Location & Seq
Forward (+)
5172 .. 7339
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021235.1_g000003.1.cds

Sequence Viewer

Length: 1161 bp
atgacttctcctgtcgaattcaaaacccagaattatttggttttgaaaccaaacaaggctggtccgatcaagctcgtacaattcttgtgggctggctacatagcggagggagaagatgctatgatattgtgcccaaagggagaggaggaaaatctcaaacgtaaatggcttatatctttcatcctcctgcttcaaatgattctgttatggattgccaatcccatgagaaactttggtttagtttttgagtggttcctcaaccttccttctagtaagcgaaactggcttgagctctctcttgatcttttaacagattatcaaggaagacatactactcaagccttcatgctccatgaccctgttgatcaattgatcgttgtggcattcagaggtaccgagttctttgatgcagatgcatggagtactgacctcgatatttcctggtacgactacaggtgcattgggaaagttcatggtgggtttatgaaagccctaggcttaacccccgaaggttttcctgacaaacaaccagaactagacgctccgtaccctttagcttactacactctcaggaatcaattgcgagatctcgtgaaagacaccaataacacgaaattcatagtgaccggccatagcttgggtggtgcactggcaattttgtttgcagcaattttggcattgcataaggaagatttgttgttggaaagattgcaaggggtttatacgttcgggcagccacgagttggggataaggactttcaaggattcatggagaaacaaatgaaaaatcataagatcaagtatcagaggatcgtttatagtaacgatttggttcctaggataccctttgatagttctacatttttgttcaagcattttgggacatgcctctactataacagcctgtataaagaaaagatgcttacagaagagccaaacaagaattactttgctccaaaggaggcaatattcaagtacctagttgctgcttgggagctcataagaagcttcataattccacacgtagaaggatgggagtacaaagaaacttggttactcaaggggattagggtatttggactcataataccaggttttgcagctcataatccccatgattatgttaatgcaacccgactggctgagtcagctgtgaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

386

Amino Acids

44.66

Weight (kDa)

7.15

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 2 cut(s) 637, 743
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 818
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 2 cut(s) 17, 614
AfaI GTAC 7 cut(s) 78, 394, 424, 446, 548, 977, 1040
AfiI CCNNNNNNNGG 2 cut(s) 637, 743
AflIII ACRYGT 1 cut(s) 1021
AgsI TTSAA 6 cut(s) 22, 46, 194, 761, 871, 973
AjnI CCWGG 2 cut(s) 440, 1090
AluBI AGCT 8 cut(s) 73, 292, 557, 636, 997, 1008, 1103, 1151
AluI AGCT 8 cut(s) 73, 292, 557, 636, 997, 1008, 1103, 1151
Alw21I GWGCWC 3 cut(s) 294, 649, 999
Alw44I GTGCAC 1 cut(s) 645
AlwI GGATC 1 cut(s) 818
AoxI GGCC 1 cut(s) 628
ApaLI GTGCAC 1 cut(s) 645
ApeKI GCWGC 4 cut(s) 665, 733, 986, 1100
ApoI RAATTY 2 cut(s) 17, 614
Asp700I GAANNNNTTC 1 cut(s) 513
Asp718I GGTACC 1 cut(s) 392
AspA2I CCTAGG 2 cut(s) 493, 836
AspS9I GGNCC 1 cut(s) 62
AvaII GGWCC 1 cut(s) 62
AvrII CCTAGG 2 cut(s) 493, 836
BaeGI GKGCMC 2 cut(s) 134, 649
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 2 cut(s) 294, 999
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BauI CACGAG 2 cut(s) 590, 738
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 3 cut(s) 294, 649, 999
BbvI GCAGC 4 cut(s) 677, 745, 973, 1112
BccI CCATC 1 cut(s) 1026
BciT130I CCWGG 2 cut(s) 442, 1092
BciVI GTATCC 1 cut(s) 834
BclI TGATCA 1 cut(s) 364
BfaI CTAG 5 cut(s) 270, 494, 536, 837, 980
BfmI CTRYAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 834
BglII AGATCT 1 cut(s) 586
BisI GCNGC 4 cut(s) 666, 734, 987, 1101
BlnI CCTAGG 2 cut(s) 493, 836
BlsI GCNGC 4 cut(s) 667, 735, 988, 1102
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 442, 1092
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 3 cut(s) 254, 394, 834
BmrFI CCNGG 2 cut(s) 442, 1092
BmsI GCATC 4 cut(s) 106, 397, 403, 909
BpiI GAAGAC 1 cut(s) 331
BpuEI CTTGAG 3 cut(s) 308, 321, 1043
BsaAI YACGTR 1 cut(s) 1024
BsaJI CCNNGG 2 cut(s) 493, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 637, 743
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 3 cut(s) 287, 654, 1143
Bse3DI GCAATG 1 cut(s) 677
BseBI CCWGG 2 cut(s) 442, 1092
BseDI CCNNGG 2 cut(s) 493, 836
BseGI GGATG 2 cut(s) 180, 1037
BseLI CCNNNNNNNGG 2 cut(s) 637, 743
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 2 cut(s) 583, 1134
BseNI ACTGG 3 cut(s) 287, 654, 1143
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 649
BseXI GCAGC 4 cut(s) 677, 745, 973, 1112
BshFI GGCC 1 cut(s) 630
BshNI GGYRCC 1 cut(s) 392
BsiHKAI GWGCWC 3 cut(s) 294, 649, 999
BsiSI CCGG 1 cut(s) 627
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 2 cut(s) 637, 743
BsmFI GGGAC 1 cut(s) 895
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 1 cut(s) 630
Bsp1286I GDGCHC 4 cut(s) 134, 294, 649, 999
Bsp143I GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 630
BspCNI CTCAG 2 cut(s) 582, 1135
BspLI GGNNCC 3 cut(s) 254, 394, 834
BspPI GGATC 1 cut(s) 818
BspQI GCTCTTC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 3 cut(s) 287, 654, 1143
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 2 cut(s) 493, 836
BssMI GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
BssSI CACGAG 2 cut(s) 590, 738
BssT1I CCWWGG 2 cut(s) 493, 836
Bst2BI CACGAG 2 cut(s) 590, 738
Bst2UI CCWGG 2 cut(s) 442, 1092
Bst6I CTCTTC 1 cut(s) 924
BstBAI YACGTR 1 cut(s) 1024
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 569, 1143
BstF5I GGATG 2 cut(s) 180, 1037
BstKTI GATC 7 cut(s) 69, 304, 367, 375, 589, 798, 813
BstMBI GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
BstMWI GCNNNNNNNGC 3 cut(s) 283, 674, 1148
BstNI CCWGG 2 cut(s) 442, 1092
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 2 cut(s) 440, 1090
BstSFI CTRYAG 1 cut(s) 451
BstSLI GKGCMC 2 cut(s) 134, 649
BstV1I GCAGC 4 cut(s) 677, 745, 973, 1112
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuI GTATCC 1 cut(s) 834
BsuRI GGCC 1 cut(s) 630
BtsCI GGATG 2 cut(s) 180, 1037
BtsIMutI CAGTG 1 cut(s) 647
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 1 cut(s) 548
CsiI ACCWGGT 1 cut(s) 1090
Csp6I GTAC 7 cut(s) 77, 393, 423, 445, 547, 976, 1039
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 8 cut(s) 223, 346, 353, 417, 473, 769, 885, 1115
CviQI GTAC 7 cut(s) 77, 393, 423, 445, 547, 976, 1039
DdeI CTNAG 2 cut(s) 569, 1143
DpnI GATC 7 cut(s) 68, 303, 366, 374, 588, 797, 812
DpnII GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 924
EarI CTCTTC 1 cut(s) 924
Ecl136II GAGCTC 2 cut(s) 292, 997
Eco130I CCWWGG 2 cut(s) 493, 836
Eco24I GRGCYC 2 cut(s) 294, 999
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 2 cut(s) 292, 997
EcoICRI GAGCTC 2 cut(s) 292, 997
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 440, 1090
EcoT14I CCWWGG 2 cut(s) 493, 836
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 2 cut(s) 294, 999
ErhI CCWWGG 2 cut(s) 493, 836
FaeI CATG 8 cut(s) 226, 349, 356, 420, 476, 772, 888, 1118
FalI AAGNNNNNCTT 2 cut(s) 932, 964
FaqI GGGAC 1 cut(s) 895
FatI CATG 8 cut(s) 222, 345, 352, 416, 472, 768, 884, 1114
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 666, 734, 987, 1101
FokI GGATG 2 cut(s) 167, 1044
FriOI GRGCYC 2 cut(s) 294, 999
Fsp4HI GCNGC 4 cut(s) 666, 734, 987, 1101
FspBI CTAG 5 cut(s) 270, 494, 536, 837, 980
GluI GCNGC 4 cut(s) 666, 734, 987, 1101
HaeIII GGCC 1 cut(s) 630
HapII CCGG 1 cut(s) 627
HgaI GACGC 1 cut(s) 548
Hin1II CATG 8 cut(s) 226, 349, 356, 420, 476, 772, 888, 1118
HindIII AAGCTT 1 cut(s) 1006
HinfI GANTC 5 cut(s) 199, 574, 765, 1080, 1145
HpaII CCGG 1 cut(s) 627
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 3 cut(s) 66, 389, 807
Hpy188III TCNNGA 4 cut(s) 299, 518, 571, 592
Hpy8I GTNNAC 1 cut(s) 647
HpyAV CCTTC 5 cut(s) 272, 276, 352, 503, 1022
HpyCH4IV ACGT 3 cut(s) 160, 725, 1023
HpyCH4V TGCA 9 cut(s) 410, 416, 459, 647, 665, 682, 712, 1100, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 674, 1148
HpyF3I CTNAG 2 cut(s) 569, 1143
HpySE526I ACGT 3 cut(s) 160, 725, 1023
Hsp92II CATG 8 cut(s) 226, 349, 356, 420, 476, 772, 888, 1118
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
LguI GCTCTTC 1 cut(s) 924
LmnI GCTCC 4 cut(s) 354, 547, 958, 994
Lsp1109I GCAGC 4 cut(s) 677, 745, 973, 1112
LweI GCATC 4 cut(s) 106, 397, 403, 909
MabI ACCWGGT 1 cut(s) 1090
MaeI CTAG 5 cut(s) 270, 494, 536, 837, 980
MaeII ACGT 3 cut(s) 160, 725, 1023
MaeIII GTNAC 3 cut(s) 622, 821, 1053
MalI GATC 7 cut(s) 68, 303, 366, 374, 588, 797, 812
MboI GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
MboII GAAGA 4 cut(s) 125, 336, 701, 941
MfeI CAATTG 2 cut(s) 368, 578
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 4 cut(s) 134, 294, 649, 999
MlyI GAGTC 2 cut(s) 1074, 1154
MmeI TCCRAC 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 3 cut(s) 308, 500, 1125
MslI CAYNNNNRTG 1 cut(s) 1119
MspA1I CMGCKG 1 cut(s) 1151
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 2 cut(s) 442, 1092
MunI CAATTG 2 cut(s) 368, 578
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 2 cut(s) 442, 1092
MwoI GCNNNNNNNGC 3 cut(s) 283, 674, 1148
NdeII GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
NlaIII CATG 8 cut(s) 226, 349, 356, 420, 476, 772, 888, 1118
NlaIV GGNNCC 3 cut(s) 254, 394, 834
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 1 cut(s) 888
PciSI GCTCTTC 1 cut(s) 924
PctI GAATGC 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 199, 574, 765
PflMI CCANNNNNTGG 2 cut(s) 637, 743
PkrI GCNGC 4 cut(s) 667, 735, 988, 1102
PleI GAGTC 2 cut(s) 1074, 1153
PpsI GAGTC 2 cut(s) 1074, 1153
Ppu21I YACGTR 1 cut(s) 1024
Psp124BI GAGCTC 2 cut(s) 294, 999
Psp6I CCWGG 2 cut(s) 440, 1090
PspGI CCWGG 2 cut(s) 440, 1090
PspN4I GGNNCC 3 cut(s) 254, 394, 834
PspPI GGNCC 1 cut(s) 62
PsuI RGATCY 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 7 cut(s) 78, 394, 424, 446, 548, 977, 1040
RsaNI GTAC 7 cut(s) 77, 393, 423, 445, 547, 976, 1039
RseI CAYNNNNRTG 1 cut(s) 1119
SacI GAGCTC 2 cut(s) 294, 999
SapI GCTCTTC 1 cut(s) 924
SaqAI TTAA 3 cut(s) 308, 500, 1125
SatI GCNGC 4 cut(s) 666, 734, 987, 1101
Sau3AI GATC 7 cut(s) 66, 301, 364, 372, 586, 795, 810
Sau96I GGNCC 1 cut(s) 62
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 2 cut(s) 1074, 1154
ScrFI CCNGG 2 cut(s) 442, 1092
SduI GDGCHC 4 cut(s) 134, 294, 649, 999
SexAI ACCWGGT 1 cut(s) 1090
SfaNI GCATC 4 cut(s) 106, 397, 403, 909
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 1119
SmlI CTYRAG 3 cut(s) 287, 336, 1058
SmoI CTYRAG 3 cut(s) 287, 336, 1058
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 969
SspMI CTAG 5 cut(s) 270, 494, 536, 837, 980
SstI GAGCTC 2 cut(s) 294, 999
StyD4I CCNGG 2 cut(s) 440, 1090
StyI CCWWGG 2 cut(s) 493, 836
TaiI ACGT 3 cut(s) 163, 728, 1026
TaqI TCGA 2 cut(s) 15, 432
TatI WGTACW 2 cut(s) 422, 1038
TfiI GAWTC 3 cut(s) 199, 574, 765
Tru1I TTAA 3 cut(s) 308, 500, 1125
Tru9I TTAA 3 cut(s) 308, 500, 1125
TscAI CASTG 1 cut(s) 654
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 4 cut(s) 665, 733, 986, 1100
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 8 cut(s) 169, 334, 461, 500, 607, 757, 797, 1000
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 1 cut(s) 654
Van91I CCANNNNNTGG 2 cut(s) 637, 743
VneI GTGCAC 1 cut(s) 645
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 2 cut(s) 17, 614
XceI RCATGY 1 cut(s) 888
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmaJI CCTAGG 2 cut(s) 493, 836
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 5 cut(s) 270, 494, 536, 837, 980
ZrmI AGTACT 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.