Rh2DG187300

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013471
Physical Location & Seq
Reverse (-)
16716507 .. 16719956
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG187300.1

Sequence Viewer

Length: 1260 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGGCTGGCTACATAGCGGAGGGAGAAGATGCTATGATTTTGTGCCCAAAGGGAGAGGAGGAAAATCTCAAACGTAAATGGCTTATATCTTTCATCCTCCTGCTTCAAATGATTCTGTTATGGATTGCCAAACCCATGAGAAACTTTGGTTTAGTTTTTGAGTGGTTCCTCAACCTTCCTTCTAGTAAGCGAAACTGGCTTGAGCTCTCTCTTGATCTTTTAACAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTTAGATGTCAGATATAGAAGAGCACTCTATGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCATTGGAAGATTATCAAGGAAGACATACTACTCAAGCCTTCATGCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCGAAGGTTTTCCTGACAAAGAACCAGAACTAGACGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTACGAGATCTCGTGAAAGATACCAATAACACGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGATACCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACATGCCTCTACTATAACAGCCTGTATAAAGAAAAGATACTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATATTCAAGTACCTAGTTGCTGCTTGGGAGCTCATAAGAAGCTTCATAATTCCACACAGAAATTGGTCCCTCAAGGAACGAATGGGAGCGAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

419

Amino Acids

48.48

Weight (kDa)

8.92

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 191 - 349 7.9e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 587
AccB7I CCANNNNNTGG 2 cut(s) 832, 938
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 1013
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 2 cut(s) 17, 809
AfaI GTAC 6 cut(s) 78, 589, 619, 641, 743, 1172
AfiI CCNNNNNNNGG 2 cut(s) 832, 938
AgsI TTSAA 7 cut(s) 22, 46, 194, 479, 956, 1066, 1168
AjnI CCWGG 1 cut(s) 635
AluBI AGCT 6 cut(s) 73, 292, 752, 831, 1192, 1203
AluI AGCT 6 cut(s) 73, 292, 752, 831, 1192, 1203
Alw21I GWGCWC 4 cut(s) 294, 430, 844, 1194
Alw44I GTGCAC 1 cut(s) 840
AlwI GGATC 1 cut(s) 1013
AoxI GGCC 1 cut(s) 823
ApaLI GTGCAC 1 cut(s) 840
ApeKI GCWGC 4 cut(s) 443, 860, 928, 1181
ApoI RAATTY 2 cut(s) 17, 809
Asp700I GAANNNNTTC 1 cut(s) 708
Asp718I GGTACC 1 cut(s) 587
AspA2I CCTAGG 2 cut(s) 688, 1031
AspS9I GGNCC 2 cut(s) 62, 1227
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 2 cut(s) 62, 1227
AvrII CCTAGG 2 cut(s) 688, 1031
BaeGI GKGCMC 2 cut(s) 134, 844
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 294, 1194
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BauI CACGAG 2 cut(s) 785, 933
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 4 cut(s) 294, 430, 844, 1194
BbvI GCAGC 4 cut(s) 430, 872, 940, 1168
BciT130I CCWGG 1 cut(s) 637
BciVI GTATCC 1 cut(s) 1029
BclI TGATCA 1 cut(s) 559
BfaI CTAG 5 cut(s) 270, 689, 731, 1032, 1175
BfmI CTRYAG 1 cut(s) 646
BfuI GTATCC 1 cut(s) 1029
BglII AGATCT 1 cut(s) 781
BisI GCNGC 4 cut(s) 444, 861, 929, 1182
BlnI CCTAGG 2 cut(s) 688, 1031
BlsI GCNGC 4 cut(s) 445, 862, 930, 1183
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 637
Bme18I GGWCC 2 cut(s) 62, 1227
BmgT120I GGNCC 2 cut(s) 62, 1227
BmiI GGNNCC 4 cut(s) 254, 589, 1029, 1229
BmrFI CCNGG 1 cut(s) 637
BmsI GCATC 4 cut(s) 106, 405, 592, 598
BpiI GAAGAC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 3 cut(s) 308, 512, 1217
BsaJI CCNNGG 2 cut(s) 688, 1031
BsaXI ACNNNNNCTCC 1 cut(s) 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 832, 938
Bse118I RCCGGY 2 cut(s) 402, 821
Bse1I ACTGG 2 cut(s) 287, 849
Bse3DI GCAATG 2 cut(s) 444, 872
BseBI CCWGG 1 cut(s) 637
BseDI CCNNGG 2 cut(s) 688, 1031
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 832, 938
BseMI GCAATG 2 cut(s) 444, 872
BseMII CTCAG 2 cut(s) 351, 778
BseNI ACTGG 2 cut(s) 287, 849
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 844
BseXI GCAGC 4 cut(s) 430, 872, 940, 1168
BshFI GGCC 1 cut(s) 825
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 4 cut(s) 294, 430, 844, 1194
BsiSI CCGG 2 cut(s) 403, 822
BslFI GGGAC 2 cut(s) 1090, 1213
BslI CCNNNNNNNGG 2 cut(s) 832, 938
BsmFI GGGAC 2 cut(s) 1090, 1213
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 1 cut(s) 825
Bsp1286I GDGCHC 5 cut(s) 134, 294, 430, 844, 1194
Bsp143I GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 825
BspCNI CTCAG 2 cut(s) 350, 777
BspLI GGNNCC 4 cut(s) 254, 589, 1029, 1229
BspPI GGATC 1 cut(s) 1013
BspQI GCTCTTC 2 cut(s) 418, 1119
BspT107I GGYRCC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 444, 872
BsrFI RCCGGY 2 cut(s) 402, 821
BsrI ACTGG 2 cut(s) 287, 849
BssAI RCCGGY 2 cut(s) 402, 821
BssECI CCNNGG 2 cut(s) 688, 1031
BssMI GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
BssSI CACGAG 2 cut(s) 785, 933
BssT1I CCWWGG 2 cut(s) 688, 1031
Bst2BI CACGAG 2 cut(s) 785, 933
Bst2UI CCWGG 1 cut(s) 637
Bst4CI ACNGT 2 cut(s) 358, 487
Bst6I CTCTTC 2 cut(s) 418, 1119
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstC8I GCNNGC 2 cut(s) 94, 542
BstDEI CTNAG 2 cut(s) 337, 764
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 7 cut(s) 69, 304, 562, 570, 784, 993, 1008
BstMBI GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
BstMWI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 869
BstNI CCWGG 1 cut(s) 637
BstNSI RCATGY 2 cut(s) 544, 1083
BstSCI CCNGG 1 cut(s) 635
BstSFI CTRYAG 1 cut(s) 646
BstSLI GKGCMC 2 cut(s) 134, 844
BstV1I GCAGC 4 cut(s) 430, 872, 940, 1168
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 1029
BsuRI GGCC 1 cut(s) 825
BtsCI GGATG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 354, 842
Cac8I GCNNGC 2 cut(s) 94, 542
Cfr10I RCCGGY 2 cut(s) 402, 821
Cfr13I GGNCC 2 cut(s) 62, 1227
CseI GACGC 1 cut(s) 743
Csp6I GTAC 6 cut(s) 77, 588, 618, 640, 742, 1171
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 8 cut(s) 223, 537, 541, 548, 612, 668, 964, 1080
CviQI GTAC 6 cut(s) 77, 588, 618, 640, 742, 1171
DdeI CTNAG 2 cut(s) 337, 764
DpnI GATC 7 cut(s) 68, 303, 561, 569, 783, 992, 1007
DpnII GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
EaeI YGGCCR 1 cut(s) 823
Eam1104I CTCTTC 2 cut(s) 418, 1119
EarI CTCTTC 2 cut(s) 418, 1119
Ecl136II GAGCTC 2 cut(s) 292, 1192
Eco130I CCWWGG 2 cut(s) 688, 1031
Eco24I GRGCYC 2 cut(s) 294, 1194
Eco47I GGWCC 2 cut(s) 62, 1227
Eco53kI GAGCTC 2 cut(s) 292, 1192
EcoICRI GAGCTC 2 cut(s) 292, 1192
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 635
EcoT14I CCWWGG 2 cut(s) 688, 1031
EcoT22I ATGCAT 2 cut(s) 542, 613
EcoT38I GRGCYC 2 cut(s) 294, 1194
ErhI CCWWGG 2 cut(s) 688, 1031
FaeI CATG 8 cut(s) 226, 540, 544, 551, 615, 671, 967, 1083
FalI AAGNNNNNCTT 2 cut(s) 1127, 1159
FaqI GGGAC 2 cut(s) 1090, 1213
FatI CATG 8 cut(s) 222, 536, 540, 547, 611, 667, 963, 1079
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 444, 861, 929, 1182
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 2 cut(s) 294, 1194
Fsp4HI GCNGC 4 cut(s) 444, 861, 929, 1182
FspBI CTAG 5 cut(s) 270, 689, 731, 1032, 1175
GluI GCNGC 4 cut(s) 444, 861, 929, 1182
HaeIII GGCC 1 cut(s) 825
HapII CCGG 2 cut(s) 403, 822
HgaI GACGC 1 cut(s) 743
Hin1II CATG 8 cut(s) 226, 540, 544, 551, 615, 671, 967, 1083
HindIII AAGCTT 1 cut(s) 1201
HinfI GANTC 4 cut(s) 199, 335, 769, 960
HpaII CCGG 2 cut(s) 403, 822
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 842
Hpy188I TCNGA 4 cut(s) 66, 416, 584, 1002
Hpy188III TCNNGA 4 cut(s) 299, 713, 766, 787
Hpy8I GTNNAC 1 cut(s) 842
HpyAV CCTTC 5 cut(s) 272, 276, 481, 543, 698
HpyCH4III ACNGT 2 cut(s) 358, 487
HpyCH4IV ACGT 2 cut(s) 160, 920
HpyCH4V TGCA 9 cut(s) 437, 540, 605, 611, 654, 842, 860, 877, 907
HpyF10VI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 869
HpyF3I CTNAG 2 cut(s) 337, 764
HpySE526I ACGT 2 cut(s) 160, 920
Hsp92II CATG 8 cut(s) 226, 540, 544, 551, 615, 671, 967, 1083
KpnI GGTACC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
LguI GCTCTTC 2 cut(s) 418, 1119
LmnI GCTCC 5 cut(s) 549, 742, 1153, 1189, 1247
Lsp1109I GCAGC 4 cut(s) 430, 872, 940, 1168
LweI GCATC 4 cut(s) 106, 405, 592, 598
MaeI CTAG 5 cut(s) 270, 689, 731, 1032, 1175
MaeII ACGT 2 cut(s) 160, 920
MaeIII GTNAC 3 cut(s) 314, 817, 1016
MalI GATC 7 cut(s) 68, 303, 561, 569, 783, 992, 1007
MboI GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
MboII GAAGA 6 cut(s) 125, 435, 514, 527, 896, 1136
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 5 cut(s) 134, 294, 430, 844, 1194
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 3 cut(s) 340, 366, 876
Mph1103I ATGCAT 2 cut(s) 542, 613
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 2 cut(s) 308, 695
MspI CCGG 2 cut(s) 403, 822
MspR9I CCNGG 1 cut(s) 637
MunI CAATTG 1 cut(s) 563
Mva1269I GAATGC 1 cut(s) 578
MvaI CCWGG 1 cut(s) 637
MwoI GCNNNNNNNGC 5 cut(s) 283, 434, 443, 452, 869
NdeI CATATG 1 cut(s) 457
NdeII GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
NlaIII CATG 8 cut(s) 226, 540, 544, 551, 615, 671, 967, 1083
NlaIV GGNNCC 4 cut(s) 254, 589, 1029, 1229
NmuCI GTSAC 2 cut(s) 314, 817
NsiI ATGCAT 2 cut(s) 542, 613
NspI RCATGY 2 cut(s) 544, 1083
PaeI GCATGC 1 cut(s) 544
PciSI GCTCTTC 2 cut(s) 418, 1119
PctI GAATGC 1 cut(s) 578
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 3 cut(s) 199, 769, 960
PflMI CCANNNNNTGG 2 cut(s) 832, 938
PkrI GCNGC 4 cut(s) 445, 862, 930, 1183
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Psp124BI GAGCTC 2 cut(s) 294, 1194
Psp6I CCWGG 1 cut(s) 635
PspGI CCWGG 1 cut(s) 635
PspN4I GGNNCC 4 cut(s) 254, 589, 1029, 1229
PspPI GGNCC 2 cut(s) 62, 1227
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 6 cut(s) 78, 589, 619, 641, 743, 1172
RsaNI GTAC 6 cut(s) 77, 588, 618, 640, 742, 1171
SacI GAGCTC 2 cut(s) 294, 1194
SapI GCTCTTC 2 cut(s) 418, 1119
SaqAI TTAA 2 cut(s) 308, 695
SatI GCNGC 4 cut(s) 444, 861, 929, 1182
Sau3AI GATC 7 cut(s) 66, 301, 559, 567, 781, 990, 1005
Sau96I GGNCC 2 cut(s) 62, 1227
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 637
SduI GDGCHC 5 cut(s) 134, 294, 430, 844, 1194
SfaNI GCATC 4 cut(s) 106, 405, 592, 598
SfcI CTRYAG 1 cut(s) 646
SinI GGWCC 2 cut(s) 62, 1227
SmlI CTYRAG 3 cut(s) 287, 527, 1232
SmoI CTYRAG 3 cut(s) 287, 527, 1232
SphI GCATGC 1 cut(s) 544
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 1164
SspMI CTAG 5 cut(s) 270, 689, 731, 1032, 1175
SstI GAGCTC 2 cut(s) 294, 1194
StyD4I CCNGG 1 cut(s) 635
StyI CCWWGG 2 cut(s) 688, 1031
TaaI ACNGT 2 cut(s) 358, 487
TaiI ACGT 2 cut(s) 163, 923
TaqI TCGA 2 cut(s) 15, 627
TatI WGTACW 1 cut(s) 617
TfiI GAWTC 3 cut(s) 199, 769, 960
Tru1I TTAA 2 cut(s) 308, 695
Tru9I TTAA 2 cut(s) 308, 695
TscAI CASTG 2 cut(s) 361, 849
TseFI GTSAC 2 cut(s) 314, 817
TseI GCWGC 4 cut(s) 443, 860, 928, 1181
Tsp45I GTSAC 2 cut(s) 314, 817
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 2 cut(s) 361, 849
Van91I CCANNNNNTGG 2 cut(s) 832, 938
VneI GTGCAC 1 cut(s) 840
VpaK11BI GGWCC 2 cut(s) 62, 1227
XapI RAATTY 2 cut(s) 17, 809
XceI RCATGY 2 cut(s) 544, 1083
XcmI CCANNNNNNNNNTGG 2 cut(s) 833, 1221
XmaJI CCTAGG 2 cut(s) 688, 1031
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 5 cut(s) 270, 689, 731, 1032, 1175
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 2 cut(s) 542, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.