RLG00000028698

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013471
Physical Location & Seq
Reverse (-)
26594370 .. 26596534
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028698

Sequence Viewer

Length: 1395 bp
ATGAGTACCATGACTTCTCCTGTCGAATTCAGAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGTCTGGCTACATAGCGGAGGGAGAAGATGCTATGATATTGTGCCCAAAGGGAGAGGATGAAAATCTCAAACGTAAATGGCTTATATCTTTCACCCTCCTGCTTCAAATGATTCTGTTATGGATTGCCAAACCCATGAGAAACTTTGGTTTAGTTTTTGAGTGGTTTCTCAACCTTCCCTCTAGCAACCGAAACTGGCTTGAGCTCTCTCTTGATCTTTTAACAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCATTGTTGGTCATACAGACAAGAGAGTGAAGTTGGATCGTAGCATCACCCGGTTAGATGTGAGATATAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAATCCTTCATTGAAACTACAGTAAAAGATCATTGGAAGATGGAACTAATCAAGTTCTATGACTTTTATAATTATTATCAAGGAAGATATACTACACAAGCCTTCATGCTCCATGACCCTGTTGATCAATTTATCGTTGTGGCATTCAGAGGTACGGAGTTCTTTGATGCAGATGCGTGGAGTACTGACCTCGATATTTCCTGGTACAAATACGGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCAAAGGCTTTCCTGATAAAGAACCAAAACTAGACCCTCCGTACCCTTTAGCTTACTACACTCTTAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCGATTTTGGCATTGCATGAGGAAGATTTGTTGTTGAAAAGATTGCAAGGGGTTTATACGTTTGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATGAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGGTACCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACCTGCCTCTACTATAACAGCCTGTACAAAGAAAAGATACTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAATGGAGGCAATATTGAAGTACCTAGTTGCTGTTTGGGAGCTCATAAGAAGCTTCATAATTCCACACGTAGAAGGATGGGAGTACAAAGAAACTTGGTTACTCAAGGGGATTAGGGTAGTTGGACTCATAATACCAGGTTTTGCAGCTCATAATCCCCATGATTATGTTAATGCAACGCGACTGGCTGAGTCAACTGTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

465

Amino Acids

53.83

Weight (kDa)

8.82

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 204 - 362 2.2e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 1075
AccB1I GGYRCC 1 cut(s) 1075
AccB7I CCANNNNNTGG 2 cut(s) 871, 977
AccII CGCG 1 cut(s) 1369
AciI CCGC 1 cut(s) 113
AclWI GGATC 2 cut(s) 405, 1052
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 2 cut(s) 26, 848
AfiI CCNNNNNNNGG 2 cut(s) 871, 977
AflIII ACRYGT 1 cut(s) 1255
AgsI TTSAA 7 cut(s) 55, 203, 488, 937, 995, 1105, 1207
AjnI CCWGG 2 cut(s) 674, 1324
AluBI AGCT 7 cut(s) 82, 301, 791, 870, 1231, 1242, 1337
AluI AGCT 7 cut(s) 82, 301, 791, 870, 1231, 1242, 1337
Alw21I GWGCWC 4 cut(s) 303, 439, 883, 1233
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 2 cut(s) 405, 1052
AoxI GGCC 1 cut(s) 862
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 4 cut(s) 452, 899, 967, 1334
ApoI RAATTY 2 cut(s) 26, 848
Asp718I GGTACC 1 cut(s) 1075
AspA2I CCTAGG 2 cut(s) 727, 1070
AspS9I GGNCC 2 cut(s) 71, 1116
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 181, 335, 400
AvaII GGWCC 2 cut(s) 71, 1116
AvrII CCTAGG 2 cut(s) 727, 1070
BaeGI GKGCMC 2 cut(s) 143, 883
BanI GGYRCC 1 cut(s) 1075
BanII GRGCYC 2 cut(s) 303, 1233
BarI GAAGNNNNNNTAC 3 cut(s) 30, 550, 582
BauI CACGAG 2 cut(s) 824, 972
Bbv12I GWGCWC 4 cut(s) 303, 439, 883, 1233
BbvI GCAGC 4 cut(s) 439, 911, 979, 1346
BccI CCATC 2 cut(s) 508, 1260
BciT130I CCWGG 2 cut(s) 676, 1326
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 1 cut(s) 412
BfaI CTAG 5 cut(s) 279, 728, 770, 1071, 1214
BfmI CTRYAG 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 1127
BglII AGATCT 1 cut(s) 820
BisI GCNGC 4 cut(s) 453, 900, 968, 1335
BlnI CCTAGG 2 cut(s) 727, 1070
BlsI GCNGC 4 cut(s) 454, 901, 969, 1336
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 3 cut(s) 412, 676, 1326
Bme18I GGWCC 2 cut(s) 71, 1116
BmgT120I GGNCC 2 cut(s) 71, 1116
BmiI GGNNCC 3 cut(s) 1068, 1077, 1117
BmrFI CCNGG 3 cut(s) 412, 676, 1326
BmsI GCATC 4 cut(s) 115, 414, 631, 637
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpuEI CTTGAG 2 cut(s) 317, 1277
BpuMI CCSGG 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 1258
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 727, 1070
Bsc4I CCNNNNNNNGG 2 cut(s) 871, 977
Bse118I RCCGGY 1 cut(s) 860
Bse1I ACTGG 3 cut(s) 296, 888, 1377
Bse3DI GCAATG 2 cut(s) 453, 911
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 2 cut(s) 676, 1326
BseDI CCNNGG 2 cut(s) 727, 1070
BseGI GGATG 2 cut(s) 160, 1271
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 871, 977
BseMI GCAATG 2 cut(s) 453, 911
BseMII CTCAG 2 cut(s) 360, 1368
BseNI ACTGG 3 cut(s) 296, 888, 1377
BseSI GKGCMC 2 cut(s) 143, 883
BseXI GCAGC 4 cut(s) 439, 911, 979, 1346
Bsh1236I CGCG 1 cut(s) 1369
BshFI GGCC 1 cut(s) 864
BshNI GGYRCC 1 cut(s) 1075
BsiHKAI GWGCWC 4 cut(s) 303, 439, 883, 1233
BsiSI CCGG 2 cut(s) 412, 861
BslFI GGGAC 1 cut(s) 1129
BslI CCNNNNNNNGG 2 cut(s) 871, 977
BsmFI GGGAC 1 cut(s) 1129
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 1 cut(s) 864
Bsp1286I GDGCHC 5 cut(s) 143, 303, 439, 883, 1233
Bsp1407I TGTACA 1 cut(s) 1140
Bsp143I GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
BspACI CCGC 1 cut(s) 113
BspANI GGCC 1 cut(s) 864
BspCNI CTCAG 2 cut(s) 359, 1369
BspFNI CGCG 1 cut(s) 1369
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 3 cut(s) 1068, 1077, 1117
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 2 cut(s) 405, 1052
BspQI GCTCTTC 2 cut(s) 427, 1158
BspT107I GGYRCC 1 cut(s) 1075
BsrDI GCAATG 2 cut(s) 453, 911
BsrFI RCCGGY 1 cut(s) 860
BsrGI TGTACA 1 cut(s) 1140
BsrI ACTGG 3 cut(s) 296, 888, 1377
BssAI RCCGGY 1 cut(s) 860
BssECI CCNNGG 2 cut(s) 727, 1070
BssMI GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
BssSI CACGAG 2 cut(s) 824, 972
BssT1I CCWWGG 2 cut(s) 727, 1070
Bst2BI CACGAG 2 cut(s) 824, 972
Bst2UI CCWGG 2 cut(s) 676, 1326
Bst4CI ACNGT 2 cut(s) 496, 1387
Bst6I CTCTTC 2 cut(s) 427, 1158
BstAPI GCANNNNNTGC 2 cut(s) 443, 452
BstAUI TGTACA 1 cut(s) 1140
BstBAI YACGTR 1 cut(s) 1258
BstDEI CTNAG 3 cut(s) 346, 803, 1377
BstF5I GGATG 2 cut(s) 160, 1271
BstFNI CGCG 1 cut(s) 1369
BstKTI GATC 8 cut(s) 78, 313, 400, 505, 601, 823, 1032, 1047
BstMBI GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
BstMWI GCNNNNNNNGC 4 cut(s) 443, 452, 461, 908
BstNI CCWGG 2 cut(s) 676, 1326
BstSCI CCNGG 3 cut(s) 410, 674, 1324
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 2 cut(s) 143, 883
BstUI CGCG 1 cut(s) 1369
BstV1I GCAGC 4 cut(s) 439, 911, 979, 1346
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BsuRI GGCC 1 cut(s) 864
BtsCI GGATG 2 cut(s) 160, 1271
BtsIMutI CAGTG 1 cut(s) 881
BveI ACCTGC 1 cut(s) 1127
CciI TCATGA 1 cut(s) 1024
Cfr10I RCCGGY 1 cut(s) 860
Cfr13I GGNCC 2 cut(s) 71, 1116
CsiI ACCWGGT 1 cut(s) 1324
CspCI CAANNNNNGTGG 2 cut(s) 77, 112
CviAII CATG 9 cut(s) 10, 232, 580, 587, 707, 917, 1003, 1025, 1349
DdeI CTNAG 3 cut(s) 346, 803, 1377
DpnI GATC 8 cut(s) 77, 312, 399, 504, 600, 822, 1031, 1046
DpnII GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 2 cut(s) 427, 1158
EarI CTCTTC 2 cut(s) 427, 1158
Ecl136II GAGCTC 2 cut(s) 301, 1231
Eco130I CCWWGG 2 cut(s) 727, 1070
Eco24I GRGCYC 2 cut(s) 303, 1233
Eco47I GGWCC 2 cut(s) 71, 1116
Eco53kI GAGCTC 2 cut(s) 301, 1231
EcoICRI GAGCTC 2 cut(s) 301, 1231
EcoO109I RGGNCCY 1 cut(s) 1116
EcoRI GAATTC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 674, 1324
EcoT14I CCWWGG 2 cut(s) 727, 1070
EcoT38I GRGCYC 2 cut(s) 303, 1233
ErhI CCWWGG 2 cut(s) 727, 1070
FaeI CATG 9 cut(s) 13, 235, 583, 590, 710, 920, 1006, 1028, 1352
FalI AAGNNNNNCTT 2 cut(s) 1166, 1198
FaqI GGGAC 1 cut(s) 1129
FatI CATG 9 cut(s) 9, 231, 579, 586, 706, 916, 1002, 1024, 1348
FauNDI CATATG 1 cut(s) 466
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 4 cut(s) 453, 900, 968, 1335
FokI GGATG 2 cut(s) 167, 1278
FriOI GRGCYC 2 cut(s) 303, 1233
Fsp4HI GCNGC 4 cut(s) 453, 900, 968, 1335
FspBI CTAG 5 cut(s) 279, 728, 770, 1071, 1214
GluI GCNGC 4 cut(s) 453, 900, 968, 1335
HaeIII GGCC 1 cut(s) 864
HapII CCGG 2 cut(s) 412, 861
Hin1II CATG 9 cut(s) 13, 235, 583, 590, 710, 920, 1006, 1028, 1352
HincII GTYRAC 1 cut(s) 1383
HindII GTYRAC 1 cut(s) 1383
HindIII AAGCTT 1 cut(s) 1240
HinfI GANTC 6 cut(s) 208, 344, 808, 999, 1314, 1379
HpaII CCGG 2 cut(s) 412, 861
HphI GGTGA 3 cut(s) 181, 335, 400
Hpy166II GTNNAC 2 cut(s) 881, 1383
Hpy188I TCNGA 4 cut(s) 32, 75, 623, 1041
Hpy188III TCNNGA 4 cut(s) 308, 752, 826, 1025
Hpy8I GTNNAC 2 cut(s) 881, 1383
HpyAV CCTTC 4 cut(s) 281, 490, 586, 1256
HpyCH4III ACNGT 2 cut(s) 496, 1387
HpyCH4IV ACGT 3 cut(s) 169, 959, 1257
HpyCH4V TGCA 9 cut(s) 446, 644, 693, 881, 899, 916, 946, 1334, 1364
HpyF10VI GCNNNNNNNGC 4 cut(s) 443, 452, 461, 908
HpyF3I CTNAG 3 cut(s) 346, 803, 1377
HpySE526I ACGT 3 cut(s) 169, 959, 1257
Hsp92II CATG 9 cut(s) 13, 235, 583, 590, 710, 920, 1006, 1028, 1352
KpnI GGTACC 1 cut(s) 1079
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
LguI GCTCTTC 2 cut(s) 427, 1158
LmnI GCTCC 3 cut(s) 588, 1192, 1228
Lsp1109I GCAGC 4 cut(s) 439, 911, 979, 1346
LweI GCATC 4 cut(s) 115, 414, 631, 637
MabI ACCWGGT 1 cut(s) 1324
MaeI CTAG 5 cut(s) 279, 728, 770, 1071, 1214
MaeII ACGT 3 cut(s) 169, 959, 1257
MaeIII GTNAC 4 cut(s) 323, 856, 1055, 1287
MalI GATC 8 cut(s) 77, 312, 399, 504, 600, 822, 1031, 1046
MboI GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
MboII GAAGA 6 cut(s) 134, 444, 523, 570, 935, 1175
MfeI CAATTG 1 cut(s) 812
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 5 cut(s) 143, 303, 439, 883, 1233
MlyI GAGTC 3 cut(s) 338, 1308, 1388
MmeI TCCRAC 2 cut(s) 375, 1291
MseI TTAA 3 cut(s) 317, 734, 1359
MslI CAYNNNNRTG 1 cut(s) 1353
MspI CCGG 2 cut(s) 412, 861
MspR9I CCNGG 3 cut(s) 412, 676, 1326
MunI CAATTG 1 cut(s) 812
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 676, 1326
MvnI CGCG 1 cut(s) 1369
MwoI GCNNNNNNNGC 4 cut(s) 443, 452, 461, 908
NciI CCSGG 1 cut(s) 412
NdeI CATATG 1 cut(s) 466
NdeII GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
NlaIII CATG 9 cut(s) 13, 235, 583, 590, 710, 920, 1006, 1028, 1352
NlaIV GGNNCC 3 cut(s) 1068, 1077, 1117
NmuCI GTSAC 2 cut(s) 323, 856
PagI TCATGA 1 cut(s) 1024
PciSI GCTCTTC 2 cut(s) 427, 1158
PctI GAATGC 1 cut(s) 617
PfeI GAWTC 3 cut(s) 208, 808, 999
PflMI CCANNNNNTGG 2 cut(s) 871, 977
PkrI GCNGC 4 cut(s) 454, 901, 969, 1336
PleI GAGTC 3 cut(s) 338, 1308, 1387
PpsI GAGTC 3 cut(s) 338, 1308, 1387
Ppu21I YACGTR 1 cut(s) 1258
PpuMI RGGWCCY 1 cut(s) 1116
PsiI TTATAA 1 cut(s) 543
Psp124BI GAGCTC 2 cut(s) 303, 1233
Psp5II RGGWCCY 1 cut(s) 1116
Psp6I CCWGG 2 cut(s) 674, 1324
PspGI CCWGG 2 cut(s) 674, 1324
PspN4I GGNNCC 3 cut(s) 1068, 1077, 1117
PspPI GGNCC 2 cut(s) 71, 1116
PspPPI RGGWCCY 1 cut(s) 1116
PsuI RGATCY 1 cut(s) 820
RseI CAYNNNNRTG 1 cut(s) 1353
SacI GAGCTC 2 cut(s) 303, 1233
SapI GCTCTTC 2 cut(s) 427, 1158
SaqAI TTAA 3 cut(s) 317, 734, 1359
SatI GCNGC 4 cut(s) 453, 900, 968, 1335
Sau3AI GATC 8 cut(s) 75, 310, 397, 502, 598, 820, 1029, 1044
Sau96I GGNCC 2 cut(s) 71, 1116
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 3 cut(s) 338, 1308, 1388
ScrFI CCNGG 3 cut(s) 412, 676, 1326
SduI GDGCHC 5 cut(s) 143, 303, 439, 883, 1233
SexAI ACCWGGT 1 cut(s) 1324
SfaNI GCATC 4 cut(s) 115, 414, 631, 637
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 2 cut(s) 71, 1116
SmiMI CAYNNNNRTG 1 cut(s) 1353
SmlI CTYRAG 2 cut(s) 296, 1292
SmoI CTYRAG 2 cut(s) 296, 1292
SsiI CCGC 1 cut(s) 113
SspI AATATT 1 cut(s) 1203
SspMI CTAG 5 cut(s) 279, 728, 770, 1071, 1214
SstI GAGCTC 2 cut(s) 303, 1233
StyD4I CCNGG 3 cut(s) 410, 674, 1324
StyI CCWWGG 2 cut(s) 727, 1070
TaaI ACNGT 2 cut(s) 496, 1387
TaiI ACGT 3 cut(s) 172, 962, 1260
TaqI TCGA 2 cut(s) 24, 666
TatI WGTACW 3 cut(s) 656, 1140, 1272
TfiI GAWTC 3 cut(s) 208, 808, 999
Tru1I TTAA 3 cut(s) 317, 734, 1359
Tru9I TTAA 3 cut(s) 317, 734, 1359
TscAI CASTG 1 cut(s) 888
TseFI GTSAC 2 cut(s) 323, 856
TseI GCWGC 4 cut(s) 452, 899, 967, 1334
Tsp45I GTSAC 2 cut(s) 323, 856
TspGWI ACGGA 2 cut(s) 644, 768
TspRI CASTG 1 cut(s) 888
Van91I CCANNNNNTGG 2 cut(s) 871, 977
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 71, 1116
XapI RAATTY 2 cut(s) 26, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmaJI CCTAGG 2 cut(s) 727, 1070
XspI CTAG 5 cut(s) 279, 728, 770, 1071, 1214
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.