RchiOBHm_Chr2g0105731

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013471
Physical Location & Seq
Reverse (-)
16988633 .. 16989902
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47991

Sequence Viewer

Length: 753 bp
ATGGTCCTGAAAAGATTACCCAAAACAAGAAGGTTCCGATCATTAATTACATGTGAGATGGATGCCCTCGTTTTCCAAATTTTTAATGTTAACTCTAAATTTGTATATATAACAAGCAATATTGTTGCAGATTATCAAGGAAGACATACTACTCAAGCCTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCGAAGGTTTTCCTGACAAAGAACCAGAACTAGATGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACATGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCATGCTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATATTCAAGTACCCAGTTGCTGCTTGGGAGCTCATAAGAAGCTTCATAATTCCAAACGTAGAAGGATGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

250

Amino Acids

28.92

Weight (kDa)

7.02

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 65 - 211 1.2e-29 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 209
AccB7I CCANNNNNTGG 2 cut(s) 454, 560
AclWI GGATC 1 cut(s) 635
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 3 cut(s) 78, 98, 431
AfaI GTAC 5 cut(s) 211, 241, 263, 365, 689
AfiI CCNNNNNNNGG 2 cut(s) 454, 560
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 2 cut(s) 578, 685
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 4 cut(s) 374, 453, 709, 720
AluI AGCT 4 cut(s) 374, 453, 709, 720
Alw21I GWGCWC 2 cut(s) 466, 711
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 698
AoxI GGCC 1 cut(s) 445
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 3 cut(s) 482, 550, 698
ApoI RAATTY 3 cut(s) 78, 98, 431
AseI ATTAAT 1 cut(s) 44
Asp700I GAANNNNTTC 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 209
AspA2I CCTAGG 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 711
BarI GAAGNNNNNNTAC 2 cut(s) 133, 165
BauI CACGAG 2 cut(s) 407, 555
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 2 cut(s) 466, 711
BbvI GCAGC 3 cut(s) 494, 562, 685
BccI CCATC 2 cut(s) 52, 738
BciT130I CCWGG 1 cut(s) 259
BclI TGATCA 1 cut(s) 181
BfaI CTAG 2 cut(s) 311, 353
BfmI CTRYAG 1 cut(s) 268
BglII AGATCT 1 cut(s) 403
BisI GCNGC 3 cut(s) 483, 551, 699
BlnI CCTAGG 1 cut(s) 310
BlsI GCNGC 3 cut(s) 484, 552, 700
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 35, 211
BmrFI CCNGG 1 cut(s) 259
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 4 cut(s) 52, 214, 220, 346
BmuI ACTGGG 1 cut(s) 686
BpiI GAAGAC 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 560
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 471, 692
Bse3DI GCAATG 1 cut(s) 494
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 2 cut(s) 67, 749
BseLI CCNNNNNNNGG 2 cut(s) 454, 560
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 2 cut(s) 471, 692
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 3 cut(s) 494, 562, 685
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 209
BsiHKAI GWGCWC 2 cut(s) 466, 711
BsiSI CCGG 1 cut(s) 444
BslI CCNNNNNNNGG 2 cut(s) 454, 560
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 1 cut(s) 447
Bsp1286I GDGCHC 2 cut(s) 466, 711
Bsp143I GATC 6 cut(s) 38, 181, 189, 403, 612, 627
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 35, 211
BspPI GGATC 1 cut(s) 635
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 2 cut(s) 471, 692
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 6 cut(s) 38, 181, 189, 403, 612, 627
BssSI CACGAG 2 cut(s) 407, 555
BssT1I CCWWGG 1 cut(s) 310
Bst2BI CACGAG 2 cut(s) 407, 555
Bst2UI CCWGG 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 636
BstDEI CTNAG 1 cut(s) 386
BstF5I GGATG 2 cut(s) 67, 749
BstKTI GATC 6 cut(s) 41, 184, 192, 406, 615, 630
BstMBI GATC 6 cut(s) 38, 181, 189, 403, 612, 627
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 3 cut(s) 494, 562, 685
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 67, 749
BtsIMutI CAGTG 1 cut(s) 464
CaiI CAGNNNCTG 1 cut(s) 698
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 5 cut(s) 210, 240, 262, 364, 688
CviAII CATG 8 cut(s) 51, 163, 170, 234, 290, 427, 586, 631
CviQI GTAC 5 cut(s) 210, 240, 262, 364, 688
DdeI CTNAG 1 cut(s) 386
DpnI GATC 6 cut(s) 40, 183, 191, 405, 614, 629
DpnII GATC 6 cut(s) 38, 181, 189, 403, 612, 627
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 709
EcoICRI GAGCTC 1 cut(s) 709
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 8 cut(s) 54, 166, 173, 237, 293, 430, 589, 634
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FatI CATG 8 cut(s) 50, 162, 169, 233, 289, 426, 585, 630
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 483, 551, 699
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 3 cut(s) 483, 551, 699
FspBI CTAG 2 cut(s) 311, 353
GluI GCNGC 3 cut(s) 483, 551, 699
HaeIII GGCC 1 cut(s) 447
HapII CCGG 1 cut(s) 444
Hin1II CATG 8 cut(s) 54, 166, 173, 237, 293, 430, 589, 634
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 2 cut(s) 391, 582
HpaI GTTAAC 1 cut(s) 91
HpaII CCGG 1 cut(s) 444
Hpy166II GTNNAC 2 cut(s) 91, 464
Hpy188I TCNGA 3 cut(s) 38, 206, 624
Hpy188III TCNNGA 4 cut(s) 7, 335, 388, 409
Hpy8I GTNNAC 2 cut(s) 91, 464
HpyAV CCTTC 4 cut(s) 24, 169, 320, 734
HpyCH4IV ACGT 2 cut(s) 542, 735
HpyCH4V TGCA 8 cut(s) 128, 227, 233, 276, 464, 482, 499, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 2 cut(s) 542, 735
Hsp92II CATG 8 cut(s) 54, 166, 173, 237, 293, 430, 589, 634
KpnI GGTACC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 181
KspAI GTTAAC 1 cut(s) 91
Kzo9I GATC 6 cut(s) 38, 181, 189, 403, 612, 627
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 4 cut(s) 171, 364, 670, 706
Lsp1109I GCAGC 3 cut(s) 494, 562, 685
LweI GCATC 4 cut(s) 52, 214, 220, 346
MaeI CTAG 2 cut(s) 311, 353
MaeII ACGT 2 cut(s) 542, 735
MaeIII GTNAC 1 cut(s) 439
MalI GATC 6 cut(s) 40, 183, 191, 405, 614, 629
MboI GATC 6 cut(s) 38, 181, 189, 403, 612, 627
MboII GAAGA 3 cut(s) 153, 518, 653
MfeI CAATTG 2 cut(s) 185, 395
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 466, 711
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 77, 200, 257, 618, 667
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 4 cut(s) 44, 84, 90, 317
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 1 cut(s) 259
MunI CAATTG 2 cut(s) 185, 395
Mva1269I GAATGC 1 cut(s) 200
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 1 cut(s) 491
NdeII GATC 6 cut(s) 38, 181, 189, 403, 612, 627
NlaIII CATG 8 cut(s) 54, 166, 173, 237, 293, 430, 589, 634
NlaIV GGNNCC 2 cut(s) 35, 211
NmuCI GTSAC 1 cut(s) 439
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 1 cut(s) 54
PciI ACATGT 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 636
PctI GAATGC 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 391, 582
PflMI CCANNNNNTGG 2 cut(s) 454, 560
PkrI GCNGC 3 cut(s) 484, 552, 700
PscI ACATGT 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 257
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 35, 211
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 5 cut(s) 211, 241, 263, 365, 689
RsaNI GTAC 5 cut(s) 210, 240, 262, 364, 688
SacI GAGCTC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 44, 84, 90, 317
SatI GCNGC 3 cut(s) 483, 551, 699
Sau3AI GATC 6 cut(s) 38, 181, 189, 403, 612, 627
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 2 cut(s) 466, 711
SfaNI GCATC 4 cut(s) 52, 214, 220, 346
SfcI CTRYAG 1 cut(s) 268
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
SspI AATATT 2 cut(s) 121, 681
SspMI CTAG 2 cut(s) 311, 353
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 1 cut(s) 310
TaiI ACGT 2 cut(s) 545, 738
TaqI TCGA 1 cut(s) 249
TatI WGTACW 1 cut(s) 239
TfiI GAWTC 2 cut(s) 391, 582
Tru1I TTAA 4 cut(s) 44, 84, 90, 317
Tru9I TTAA 4 cut(s) 44, 84, 90, 317
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 3 cut(s) 482, 550, 698
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 8 cut(s) 151, 278, 317, 424, 443, 574, 614, 712
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 1 cut(s) 471
Van91I CCANNNNNTGG 2 cut(s) 454, 560
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 44
XapI RAATTY 3 cut(s) 78, 98, 431
XceI RCATGY 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 2 cut(s) 455, 699
XmaJI CCTAGG 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 311, 353
ZrmI AGTACT 1 cut(s) 241
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.