Rorug02G0129900

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013471
Physical Location & Seq
Reverse (-)
11214269 .. 11216722
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0129900.1

Sequence Viewer

Length: 1149 bp
ATGCTTTACCAAACTCACCACCCCTCCTTTTTATTCCAATTTCATCCCCATTCTTCTCCTACCTCTGTAACCAGCACCACCACTTCTCTCTCTCCTCCTCCACCTCTACCACCCTGTCCTCCTCAGGTTGTAAGAAGTGAAATGGAATGCGTTGAAGCGGCGTTGAAGACCAGTATCAGGACGGAGATGGCCGTCAAGGAGGCCGTTTTCGACGACTTGTGGGGGTTGAACGCCCAGAACGGCGGCGTGCAGAATTGCGAGGACTTTTCCGTCGACGACCTTCTCGACTTTTCCAACGACGATGGGTTTGTGGAACAACAGGCTGAGGAGGAAGACGACAAAGGCTCTGTTTTGCCCAAGAAAGAGAGCATGGAGGAAAAGGAAAATTCAACCCCGTCTTCGTGCTTGTCGGAGAAGAATGAACTTGGGCCGGAGCCGACCGAGCCCACCAGTGAACTCAGCGTTCCGGCAGACGACTTGGAAAACCTCGAGTGGTTGTCTCATTTCGTGGAGGATTCCTTCTCGGGGTTCAACACCTCTCTCCCCGCCGGGTTTCTGGCCGTGAAGCCCAAAAACCCGACCGAGAAGAGGCCAGAACCGGAGGCTCTCAAGCCCTGTTTCAAGACTCCGGTTCCAGCCAAGGCCAGAAGCAAGAGGACCCGAACCGGCGGCCGGGTGTGGTCACTAGGGTCACCAACCTTCACCGAGTCTTCCTCAAGTTCAAGTTCGTCTTCCTCAACTTCTTCATGTCCCTCCAGCCCATGGCTCATTTACAACACCACCCAAGGCCCCGGAGCCTTCGGTTCGTCAGTAGAGAAGCCTCAGAAGAAGCCAAAAAGACCAGCAACAGAAGGCGGTTCGAGCCAGCCGCCTCGGCGGTGCAGCCACTGCGGCGTCCAGAAGACTCCGCAGTGGAGAACCGGGCCCAATGGAGCCAAGACTCTGTGTAACGCCTGCGGAGTCCGTTACAAGTCGGGTCGGCTCGTGCCCGAATACCGCCCCGCTTGTAGCCCGACTTTCTCGAGCGAGTTGCACTCCAACCACCACCGCAAGGTGATGGAGATACGCCGCAAGAAGGAAGTCCCTCCAGGCCCCGAACCCGGTCTGATGACGACAGCAGCGGCGGCTCCGGTTGTTCGTAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

382

Amino Acids

41.58

Weight (kDa)

6.18

Isoelectric Point (pI)

66.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATA PF00320 294 - 328 1.9e-16 GATA zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 762
AccI GTMKAC 1 cut(s) 273
AcoI YGGCCR 3 cut(s) 189, 558, 670
AcsI RAATTY 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 894
AfiI CCNNNNNNNGG 8 cut(s) 177, 525, 588, 672, 762, 1051, 1075, 1100
AgsI TTSAA 7 cut(s) 155, 166, 229, 390, 532, 622, 723
AjnI CCWGG 1 cut(s) 1087
Alw26I GTCTC 1 cut(s) 504
AlwNI CAGNNNCTG 1 cut(s) 888
Ama87I CYCGRG 3 cut(s) 488, 523, 1021
ApaI GGGCCC 1 cut(s) 927
ApeKI GCWGC 2 cut(s) 882, 1118
ApoI RAATTY 1 cut(s) 385
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspS9I GGNCC 6 cut(s) 428, 657, 788, 923, 924, 1091
AsuC2I CCSGG 5 cut(s) 550, 674, 792, 922, 1101
AsuHPI GGTGA 4 cut(s) 8, 684, 694, 1066
AvaI CYCGRG 3 cut(s) 488, 523, 1021
AvaII GGWCC 1 cut(s) 657
AxyI CCTNAGG 1 cut(s) 123
BaeGI GKGCMC 2 cut(s) 927, 990
BanII GRGCYC 2 cut(s) 447, 927
BauI CACGAG 1 cut(s) 983
BbsI GAAGAC 6 cut(s) 173, 339, 390, 702, 723, 908
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 894, 1130
BccI CCATC 3 cut(s) 181, 296, 1051
BceAI ACGGC 4 cut(s) 176, 188, 256, 545
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 1 cut(s) 1089
BcnI CCSGG 5 cut(s) 550, 674, 792, 922, 1101
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 1 cut(s) 686
BglI GCCNNNNNGGC 2 cut(s) 874, 891
Bme1390I CCNGG 6 cut(s) 550, 674, 792, 922, 1089, 1101
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 3 cut(s) 488, 523, 1021
BmgT120I GGNCC 6 cut(s) 428, 657, 788, 923, 924, 1091
BmiI GGNNCC 9 cut(s) 435, 633, 659, 790, 796, 925, 934, 1093, 1128
BmrFI CCNGG 6 cut(s) 550, 674, 792, 922, 1089, 1101
BpiI GAAGAC 6 cut(s) 173, 339, 390, 702, 723, 908
BplI GAGNNNNNCTC 4 cut(s) 698, 730, 1019, 1051
BpmI CTGGAG 2 cut(s) 739, 1071
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 593, 700
BpuMI CCSGG 5 cut(s) 550, 674, 792, 922, 1101
BsaHI GRCGYC 1 cut(s) 894
BsaJI CCNNGG 5 cut(s) 639, 761, 784, 790, 872
BsaWI WCCGGW 3 cut(s) 598, 628, 1129
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 8 cut(s) 177, 525, 588, 672, 762, 1051, 1075, 1100
Bse118I RCCGGY 1 cut(s) 665
Bse1I ACTGG 2 cut(s) 171, 450
Bse21I CCTNAGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 1089
BseDI CCNNGG 5 cut(s) 639, 761, 784, 790, 872
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 8 cut(s) 177, 525, 588, 672, 762, 1051, 1075, 1100
BseMII CTCAG 4 cut(s) 137, 315, 472, 836
BseNI ACTGG 2 cut(s) 171, 450
BseRI GAGGAG 4 cut(s) 84, 87, 111, 341
BseSI GKGCMC 2 cut(s) 927, 990
BseX3I CGGCCG 1 cut(s) 670
BseXI GCAGC 2 cut(s) 894, 1130
BsgI GTGCAG 2 cut(s) 269, 901
Bsh1285I CGRYCG 3 cut(s) 441, 582, 673
BsiEI CGRYCG 3 cut(s) 441, 582, 673
BsiHKCI CYCGRG 3 cut(s) 488, 523, 1021
BslFI GGGAC 2 cut(s) 735, 1067
BslI CCNNNNNNNGG 8 cut(s) 177, 525, 588, 672, 762, 1051, 1075, 1100
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 2 cut(s) 735, 1067
BsmI GAATGC 1 cut(s) 152
BsoBI CYCGRG 3 cut(s) 488, 523, 1021
Bsp120I GGGCCC 1 cut(s) 923
Bsp1286I GDGCHC 3 cut(s) 447, 927, 990
Bsp19I CCATGG 1 cut(s) 761
BspCNI CTCAG 4 cut(s) 136, 316, 471, 835
BspLI GGNNCC 9 cut(s) 435, 633, 659, 790, 796, 925, 934, 1093, 1128
BsrFI RCCGGY 1 cut(s) 665
BsrI ACTGG 2 cut(s) 171, 450
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 5 cut(s) 639, 761, 784, 790, 872
BssNI GRCGYC 1 cut(s) 894
BssSI CACGAG 1 cut(s) 983
BssT1I CCWWGG 3 cut(s) 639, 761, 784
Bst2BI CACGAG 1 cut(s) 983
Bst2UI CCWGG 1 cut(s) 1089
Bst6I CTCTTC 1 cut(s) 581
BstACI GRCGYC 1 cut(s) 894
BstAPI GCANNNNNTGC 1 cut(s) 888
BstC8I GCNNGC 3 cut(s) 248, 866, 955
BstDEI CTNAG 4 cut(s) 123, 324, 458, 822
BstDSI CCRYGG 1 cut(s) 761
BstEII GGTNACC 1 cut(s) 690
BstF5I GGATG 1 cut(s) 43
BstMAI GTCTC 1 cut(s) 504
BstMCI CGRYCG 3 cut(s) 441, 582, 673
BstMWI GCNNNNNNNGC 6 cut(s) 442, 861, 874, 888, 891, 1124
BstNI CCWGG 1 cut(s) 1089
BstPI GGTNACC 1 cut(s) 690
BstSCI CCNGG 6 cut(s) 548, 672, 790, 920, 1087, 1099
BstSLI GKGCMC 2 cut(s) 927, 990
BstV1I GCAGC 2 cut(s) 894, 1130
BstV2I GAAGAC 6 cut(s) 173, 339, 390, 702, 723, 908
BstZI CGGCCG 1 cut(s) 670
Bsu36I CCTNAGG 1 cut(s) 123
BtgI CCRYGG 1 cut(s) 761
BtsCI GGATG 1 cut(s) 43
BtsI GCAGTG 2 cut(s) 886, 917
BtsIMutI CAGTG 3 cut(s) 457, 886, 917
Cac8I GCNNGC 3 cut(s) 248, 866, 955
CaiI CAGNNNCTG 1 cut(s) 888
Cfr10I RCCGGY 1 cut(s) 665
Cfr13I GGNCC 6 cut(s) 428, 657, 788, 923, 924, 1091
CseI GACGC 1 cut(s) 883
CviAII CATG 3 cut(s) 370, 747, 762
DdeI CTNAG 4 cut(s) 123, 324, 458, 822
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 3 cut(s) 189, 558, 670
EagI CGGCCG 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
EclXI CGGCCG 1 cut(s) 670
Eco130I CCWWGG 3 cut(s) 639, 761, 784
Eco24I GRGCYC 2 cut(s) 447, 927
Eco47I GGWCC 1 cut(s) 657
Eco52I CGGCCG 1 cut(s) 670
Eco81I CCTNAGG 1 cut(s) 123
Eco88I CYCGRG 3 cut(s) 488, 523, 1021
Eco91I GGTNACC 1 cut(s) 690
EcoO109I RGGNCCY 3 cut(s) 657, 788, 1091
EcoO65I GGTNACC 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 1087
EcoT14I CCWWGG 3 cut(s) 639, 761, 784
EcoT38I GRGCYC 2 cut(s) 447, 927
ErhI CCWWGG 3 cut(s) 639, 761, 784
FaeI CATG 3 cut(s) 373, 750, 765
FaiI YATR 3 cut(s) 371, 748, 763
FalI AAGNNNNNCTT 2 cut(s) 715, 747
FaqI GGGAC 2 cut(s) 735, 1067
FatI CATG 3 cut(s) 369, 746, 761
FauI CCCGC 2 cut(s) 553, 1009
FblI GTMKAC 1 cut(s) 273
FokI GGATG 1 cut(s) 30
FriOI GRGCYC 2 cut(s) 447, 927
FspBI CTAG 1 cut(s) 686
GsuI CTGGAG 2 cut(s) 739, 1071
HgaI GACGC 1 cut(s) 883
Hin1I GRCGYC 1 cut(s) 894
Hin1II CATG 3 cut(s) 373, 750, 765
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 6 cut(s) 515, 625, 707, 904, 940, 960
HphI GGTGA 4 cut(s) 8, 684, 694, 1066
Hpy166II GTNNAC 2 cut(s) 274, 455
Hpy188I TCNGA 3 cut(s) 412, 825, 1107
Hpy188III TCNNGA 5 cut(s) 178, 284, 622, 898, 1021
Hpy8I GTNNAC 2 cut(s) 274, 455
Hpy99I CGWCG 4 cut(s) 215, 275, 278, 302
HpyAV CCTTC 6 cut(s) 290, 529, 709, 808, 845, 1069
HpyCH4V TGCA 3 cut(s) 250, 882, 1033
HpyF10VI GCNNNNNNNGC 6 cut(s) 442, 861, 874, 888, 891, 1124
HpyF3I CTNAG 4 cut(s) 123, 324, 458, 822
Hsp92I GRCGYC 1 cut(s) 894
Hsp92II CATG 3 cut(s) 373, 750, 765
LmnI GCTCC 4 cut(s) 433, 794, 932, 1132
Lsp1109I GCAGC 2 cut(s) 894, 1130
MaeI CTAG 1 cut(s) 686
MaeIII GTNAC 5 cut(s) 67, 681, 690, 947, 965
MhlI GDGCHC 3 cut(s) 447, 927, 990
MluCI AATT 3 cut(s) 38, 253, 385
MlyI GAGTC 5 cut(s) 619, 716, 898, 934, 969
MmeI TCCRAC 3 cut(s) 318, 390, 1062
MspA1I CMGCKG 1 cut(s) 1121
MspR9I CCNGG 6 cut(s) 550, 674, 792, 922, 1089, 1101
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 1089
MwoI GCNNNNNNNGC 6 cut(s) 442, 861, 874, 888, 891, 1124
NciI CCSGG 5 cut(s) 550, 674, 792, 922, 1101
NcoI CCATGG 1 cut(s) 761
NlaIII CATG 3 cut(s) 373, 750, 765
NlaIV GGNNCC 9 cut(s) 435, 633, 659, 790, 796, 925, 934, 1093, 1128
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 2 cut(s) 681, 690
PaeR7I CTCGAG 2 cut(s) 488, 1021
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 515
PflMI CCANNNNNTGG 1 cut(s) 762
PleI GAGTC 5 cut(s) 619, 715, 898, 934, 968
PpsI GAGTC 5 cut(s) 619, 715, 898, 934, 968
PpuMI RGGWCCY 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 1087
PspEI GGTNACC 1 cut(s) 690
PspGI CCWGG 1 cut(s) 1087
PspN4I GGNNCC 9 cut(s) 435, 633, 659, 790, 796, 925, 934, 1093, 1128
PspOMI GGGCCC 1 cut(s) 923
PspPI GGNCC 6 cut(s) 428, 657, 788, 923, 924, 1091
PspPPI RGGWCCY 1 cut(s) 657
PspXI VCTCGAGB 1 cut(s) 488
PstNI CAGNNNCTG 1 cut(s) 888
SalI GTCGAC 1 cut(s) 272
Sau96I GGNCC 6 cut(s) 428, 657, 788, 923, 924, 1091
SchI GAGTC 5 cut(s) 619, 716, 898, 934, 969
ScrFI CCNGG 6 cut(s) 550, 674, 792, 922, 1089, 1101
SduI GDGCHC 3 cut(s) 447, 927, 990
SetI ASST 8 cut(s) 65, 106, 129, 282, 489, 539, 701, 1056
Sfr274I CTCGAG 2 cut(s) 488, 1021
SgrDI CGTCGACG 1 cut(s) 272
SinI GGWCC 1 cut(s) 657
SlaI CTCGAG 2 cut(s) 488, 1021
SmlI CTYRAG 4 cut(s) 488, 608, 715, 1021
SmoI CTYRAG 4 cut(s) 488, 608, 715, 1021
Sse9I AATT 3 cut(s) 38, 253, 385
SspMI CTAG 1 cut(s) 686
StyD4I CCNGG 6 cut(s) 548, 672, 790, 920, 1087, 1099
StyI CCWWGG 3 cut(s) 639, 761, 784
TaqI TCGA 6 cut(s) 210, 273, 285, 489, 860, 1022
TaqII GACCGA 2 cut(s) 455, 596
TasI AATT 3 cut(s) 38, 253, 385
TauI GCSGC 8 cut(s) 161, 246, 672, 871, 894, 1071, 1124, 1127
TfiI GAWTC 1 cut(s) 515
TscAI CASTG 3 cut(s) 457, 893, 917
TseFI GTSAC 2 cut(s) 681, 690
TseI GCWGC 2 cut(s) 882, 1118
Tsp45I GTSAC 2 cut(s) 681, 690
TspDTI ATGAA 3 cut(s) 32, 435, 735
TspGWI ACGGA 3 cut(s) 197, 259, 953
TspRI CASTG 3 cut(s) 457, 893, 917
Van91I CCANNNNNTGG 1 cut(s) 762
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 1 cut(s) 385
XhoI CTCGAG 2 cut(s) 488, 1021
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.