RLG00000017454

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013471
Physical Location & Seq
Reverse (-)
16986717 .. 16988848
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017454

Sequence Viewer

Length: 741 bp
ATGACTTCTCCTTTCGAATTCATCACCGAGAATTACTTGGTTTTGAATCCACACAAGCCTGGTCCGATCAAGCTCGTACACTTCTTGTGGTCTGGCTACATAGCGGAGGGAGAAGATGCTATGATATCGTGCCCAAAGGGAGAGCTGGAGGAAACCCGCAAACGTGAAGTGAAGAAAGAGAGAGAATCAGCGAGTTTCATTTCCACTGTTGGACATACAGACGAGAGAGTGAAGTTGGATGGTAGCATCAGCCGGTCAGATGTTAGATATAGAAGAGCACTCTCTGCAAATGCAATGGCTGCTAAAATAGCATATGAGAACCGAGCCTTCATTAAAACTGCAGTCGAAGATCATTGGAAGCTAAACTACATGTATGATGTGCATTGGAAAGTTCATGGTGGGTTTATGAAAGCCCTGGGCTTTAAACCAAAATGTTTTCCTAATAAAGAATCAGAACCAGAACTAGACCCTCAGTACCCTTTAGCTTATTACACCCTTAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATTGTTTCGGGCCATAGCTTGGGTGGTGCACTGGAAATTTTGTTTGCATCGATTTTGGCATTGCATAAGGAAGAAGTATTGTTGGAAAGATTGCAAGGAGTTTATACGTTCAGGCAGCCAAGAGTTGGGGATAAGGATTTTCAAGGATCCATGGAGAAACAAATGAAACATTATAATATCGATGGGAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

247

Amino Acids

28.32

Weight (kDa)

8.65

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 128 - 239 5.6e-19 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AccB7I CCANNNNNTGG 2 cut(s) 565, 671
AciI CCGC 2 cut(s) 104, 157
AclWI GGATC 2 cut(s) 687, 700
AcsI RAATTY 4 cut(s) 17, 542, 582, 734
AfaI GTAC 2 cut(s) 78, 476
AfiI CCNNNNNNNGG 2 cut(s) 565, 671
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 2 cut(s) 46, 689
AjnI CCWGG 2 cut(s) 58, 414
AluBI AGCT 5 cut(s) 73, 145, 361, 485, 564
AluI AGCT 5 cut(s) 73, 145, 361, 485, 564
Alw21I GWGCWC 2 cut(s) 280, 577
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 2 cut(s) 687, 700
AoxI GGCC 1 cut(s) 556
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 2 cut(s) 299, 661
ApoI RAATTY 4 cut(s) 17, 542, 582, 734
AspS9I GGNCC 2 cut(s) 62, 556
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 15
AvaII GGWCC 1 cut(s) 62
AxyI CCTNAGG 1 cut(s) 497
BaeGI GKGCMC 2 cut(s) 134, 577
BamHI GGATCC 1 cut(s) 692
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
BauI CACGAG 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 280, 577
BbvI GCAGC 2 cut(s) 286, 673
BccI CCATC 2 cut(s) 233, 722
BciT130I CCWGG 2 cut(s) 60, 416
BfaI CTAG 2 cut(s) 464, 739
BfmI CTRYAG 1 cut(s) 339
BglII AGATCT 1 cut(s) 514
BisI GCNGC 2 cut(s) 300, 662
BlsI GCNGC 2 cut(s) 301, 663
Bme1390I CCNGG 2 cut(s) 60, 416
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 2 cut(s) 62, 556
BmiI GGNNCC 1 cut(s) 694
BmrFI CCNGG 2 cut(s) 60, 416
BmsI GCATC 3 cut(s) 106, 255, 602
BpmI CTGGAG 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 15
Bsa29I ATCGAT 2 cut(s) 596, 726
BsaJI CCNNGG 3 cut(s) 414, 415, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 565, 671
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 1 cut(s) 582
Bse21I CCTNAGG 1 cut(s) 497
Bse3DI GCAATG 2 cut(s) 300, 605
BseBI CCWGG 2 cut(s) 60, 416
BseCI ATCGAT 2 cut(s) 596, 726
BseDI CCNNGG 3 cut(s) 414, 415, 696
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 2 cut(s) 565, 671
BseMI GCAATG 2 cut(s) 300, 605
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 1 cut(s) 582
BseSI GKGCMC 2 cut(s) 134, 577
BseXI GCAGC 2 cut(s) 286, 673
BshFI GGCC 1 cut(s) 558
BshVI ATCGAT 2 cut(s) 596, 726
BsiHKAI GWGCWC 2 cut(s) 280, 577
BsiSI CCGG 1 cut(s) 253
BslI CCNNNNNNNGG 2 cut(s) 565, 671
BsnI GGCC 1 cut(s) 558
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 3 cut(s) 134, 280, 577
Bsp143I GATC 4 cut(s) 66, 349, 514, 692
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 2 cut(s) 104, 157
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 1 cut(s) 484
BspDI ATCGAT 2 cut(s) 596, 726
BspLI GGNNCC 1 cut(s) 694
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 2 cut(s) 687, 700
BspQI GCTCTTC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 15
BsrDI GCAATG 2 cut(s) 300, 605
BsrFI RCCGGY 1 cut(s) 252
BsrI ACTGG 1 cut(s) 582
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 3 cut(s) 414, 415, 696
BssMI GATC 4 cut(s) 66, 349, 514, 692
BssSI CACGAG 1 cut(s) 518
BssT1I CCWWGG 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 60, 416
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 268
BstAPI GCANNNNNTGC 2 cut(s) 284, 299
BstBI TTCGAA 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 471, 497
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 4 cut(s) 69, 352, 517, 695
BstMBI GATC 4 cut(s) 66, 349, 514, 692
BstMWI GCNNNNNNNGC 3 cut(s) 284, 299, 308
BstNI CCWGG 2 cut(s) 60, 416
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 2 cut(s) 58, 414
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 2 cut(s) 134, 577
BstV1I GCAGC 2 cut(s) 286, 673
BstX2I RGATCY 2 cut(s) 514, 692
BstYI RGATCY 2 cut(s) 514, 692
Bsu15I ATCGAT 2 cut(s) 596, 726
Bsu36I CCTNAGG 1 cut(s) 497
BsuRI GGCC 1 cut(s) 558
BsuTUI ATCGAT 2 cut(s) 596, 726
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 204, 575
Cfr10I RCCGGY 1 cut(s) 252
Cfr13I GGNCC 2 cut(s) 62, 556
ClaI ATCGAT 2 cut(s) 596, 726
Csp6I GTAC 2 cut(s) 77, 475
CviAII CATG 3 cut(s) 370, 395, 697
CviQI GTAC 2 cut(s) 77, 475
DdeI CTNAG 2 cut(s) 471, 497
DpnI GATC 4 cut(s) 68, 351, 516, 694
DpnII GATC 4 cut(s) 66, 349, 514, 692
DraI TTTAAA 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 696
Eco32I GATATC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 62
Eco81I CCTNAGG 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 58, 414
EcoRV GATATC 1 cut(s) 126
EcoT14I CCWWGG 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 3 cut(s) 373, 398, 700
FatI CATG 3 cut(s) 369, 394, 696
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 300, 662
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 2 cut(s) 300, 662
FspBI CTAG 2 cut(s) 464, 739
GluI GCNGC 2 cut(s) 300, 662
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 1 cut(s) 558
HapII CCGG 1 cut(s) 253
Hin1II CATG 3 cut(s) 373, 398, 700
HinfI GANTC 4 cut(s) 46, 185, 449, 502
HpaII CCGG 1 cut(s) 253
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 79, 575
Hpy188I TCNGA 3 cut(s) 66, 259, 454
Hpy188III TCNNGA 1 cut(s) 520
Hpy8I GTNNAC 2 cut(s) 79, 575
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 2 cut(s) 163, 653
HpyCH4V TGCA 8 cut(s) 287, 293, 341, 382, 575, 593, 610, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 299, 308
HpyF3I CTNAG 2 cut(s) 471, 497
HpySE526I ACGT 2 cut(s) 163, 653
Hsp92II CATG 3 cut(s) 373, 398, 700
Kzo9I GATC 4 cut(s) 66, 349, 514, 692
LguI GCTCTTC 1 cut(s) 268
Lsp1109I GCAGC 2 cut(s) 286, 673
LweI GCATC 3 cut(s) 106, 255, 602
MaeI CTAG 2 cut(s) 464, 739
MaeII ACGT 2 cut(s) 163, 653
MalI GATC 4 cut(s) 68, 351, 516, 694
MboI GATC 4 cut(s) 66, 349, 514, 692
MboII GAAGA 5 cut(s) 125, 184, 285, 359, 629
MfeI CAATTG 1 cut(s) 506
MflI RGATCY 2 cut(s) 514, 692
MhlI GDGCHC 3 cut(s) 134, 280, 577
MluCI AATT 6 cut(s) 17, 31, 506, 542, 582, 734
MmeI TCCRAC 3 cut(s) 190, 216, 609
MnlI CCTC 3 cut(s) 100, 142, 480
MseI TTAA 2 cut(s) 333, 423
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 2 cut(s) 60, 416
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 2 cut(s) 60, 416
MwoI GCNNNNNNNGC 3 cut(s) 284, 299, 308
NcoI CCATGG 1 cut(s) 696
NdeI CATATG 1 cut(s) 313
NdeII GATC 4 cut(s) 66, 349, 514, 692
NlaIII CATG 3 cut(s) 373, 398, 700
NlaIV GGNNCC 1 cut(s) 694
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 15
PasI CCCWGGG 1 cut(s) 415
PciI ACATGT 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 268
PfeI GAWTC 4 cut(s) 46, 185, 449, 502
PflMI CCANNNNNTGG 2 cut(s) 565, 671
PkrI GCNGC 2 cut(s) 301, 663
PscI ACATGT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 720
Psp6I CCWGG 2 cut(s) 58, 414
PspGI CCWGG 2 cut(s) 58, 414
PspN4I GGNNCC 1 cut(s) 694
PspPI GGNCC 2 cut(s) 62, 556
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 2 cut(s) 514, 692
RsaI GTAC 2 cut(s) 78, 476
RsaNI GTAC 2 cut(s) 77, 475
SapI GCTCTTC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 333, 423
SatI GCNGC 2 cut(s) 300, 662
Sau3AI GATC 4 cut(s) 66, 349, 514, 692
Sau96I GGNCC 2 cut(s) 62, 556
ScrFI CCNGG 2 cut(s) 60, 416
SduI GDGCHC 3 cut(s) 134, 280, 577
SetI ASST 7 cut(s) 75, 147, 166, 363, 487, 566, 656
SfaNI GCATC 3 cut(s) 106, 255, 602
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 1 cut(s) 62
Sse9I AATT 6 cut(s) 17, 31, 506, 542, 582, 734
SsiI CCGC 2 cut(s) 104, 157
SspMI CTAG 2 cut(s) 464, 739
StyD4I CCNGG 2 cut(s) 58, 414
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 2 cut(s) 166, 656
TaqI TCGA 4 cut(s) 15, 345, 596, 726
TasI AATT 6 cut(s) 17, 31, 506, 542, 582, 734
TfiI GAWTC 4 cut(s) 46, 185, 449, 502
Tru1I TTAA 2 cut(s) 333, 423
Tru9I TTAA 2 cut(s) 333, 423
TscAI CASTG 2 cut(s) 211, 582
TseI GCWGC 2 cut(s) 299, 661
TspDTI ATGAA 7 cut(s) 10, 187, 319, 383, 422, 535, 725
TspRI CASTG 2 cut(s) 211, 582
Van91I CCANNNNNTGG 2 cut(s) 565, 671
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 4 cut(s) 17, 542, 582, 734
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XspI CTAG 2 cut(s) 464, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.