RLG00000017455

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013471
Physical Location & Seq
Reverse (-)
17022302 .. 17024473
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017455

Sequence Viewer

Length: 1410 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGTCTGGCTACATAGCGAAGGGAGAAGATGCTATGATATTGTGCCCAAAGGGAGAGGAGGAAAATCTCAAACGTAAATGGCTTATATCTTTCACCCTCCTGCTTCAAATGATTCTGTTATGGATTGCCAAACCCATGAGAAACTTTGGTTTAGTTTTTGAGTGGTTCCTCAACCTTCCCTCTAGTAACCGAAACTGGCTTGAGCTCTTTCTTGAACTTTTAACAGGTGACATAAAGACAGAGAGAGACTCAGCAAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTTAGATGTCAGATACAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCATTGGAAGATGGAACTAGTCAAGTTCTATGACTTTTATAATTATTATCAGGGAAGACATACTACTCAAGCTTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACACCCGAAGGTTTTCCTGACAAAGAACCAGAACCAGACGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATAGTGACCGGCCATAGTTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGGAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAGACAAATGAAAAACCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGATTCCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACCTGCCTCTACTATAACAGCCTGTATAAAGAAAAGATACTTATAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATATTCAAGTACCCAGTTGCTGTTTGGGAGCTCATAAGAAGCTTCATAATTCCACACGTAGAAGGATGGGAGTACAGAGAAACTTGGTTACTCAAGGGGATTAGGGTATTTGGACTCATAATACCAGGTTTTGCAGCTCATAATCCCCATGATTATGTTAATGCAACCCGACTGGCTGAGTCAACTGTGAAAAAGCTTCCAAAATTAATTGAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

470

Amino Acids

54.5

Weight (kDa)

8.73

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 201 - 359 2.6e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 1118
Acc65I GGTACC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 617
AccB7I CCANNNNNTGG 2 cut(s) 862, 968
AclWI GGATC 1 cut(s) 1043
AcoI YGGCCR 1 cut(s) 853
AcsI RAATTY 2 cut(s) 17, 839
AfaI GTAC 7 cut(s) 78, 619, 649, 671, 773, 1202, 1265
AfiI CCNNNNNNNGG 2 cut(s) 862, 968
AflIII ACRYGT 1 cut(s) 1246
AgsI TTSAA 9 cut(s) 22, 46, 194, 302, 479, 986, 1096, 1198, 1403
AhlI ACTAGT 1 cut(s) 511
AjnI CCWGG 2 cut(s) 665, 1315
AluBI AGCT 8 cut(s) 73, 292, 566, 782, 1222, 1233, 1328, 1387
AluI AGCT 8 cut(s) 73, 292, 566, 782, 1222, 1233, 1328, 1387
Alw21I GWGCWC 4 cut(s) 294, 430, 874, 1224
Alw26I GTCTC 2 cut(s) 327, 994
Alw44I GTGCAC 1 cut(s) 870
AlwI GGATC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 1211
AoxI GGCC 1 cut(s) 853
ApaLI GTGCAC 1 cut(s) 870
ApeKI GCWGC 4 cut(s) 443, 890, 958, 1325
ApoI RAATTY 2 cut(s) 17, 839
AseI ATTAAT 1 cut(s) 1397
Asp700I GAANNNNTTC 1 cut(s) 738
Asp718I GGTACC 1 cut(s) 617
AspA2I CCTAGG 2 cut(s) 718, 1061
AspS9I GGNCC 2 cut(s) 62, 1107
AsuHPI GGTGA 2 cut(s) 172, 326
AvaII GGWCC 2 cut(s) 62, 1107
AvrII CCTAGG 2 cut(s) 718, 1061
BaeGI GKGCMC 2 cut(s) 134, 874
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 294, 1224
BarI GAAGNNNNNNTAC 2 cut(s) 541, 573
BauI CACGAG 2 cut(s) 815, 963
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 4 cut(s) 294, 430, 874, 1224
BbvI GCAGC 4 cut(s) 430, 902, 970, 1337
BccI CCATC 2 cut(s) 499, 1251
BciT130I CCWGG 2 cut(s) 667, 1317
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 327, 994
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 4 cut(s) 270, 512, 719, 1062
BfmI CTRYAG 1 cut(s) 676
BfuAI ACCTGC 1 cut(s) 1118
BglII AGATCT 1 cut(s) 811
BisI GCNGC 4 cut(s) 444, 891, 959, 1326
BlnI CCTAGG 2 cut(s) 718, 1061
BlsI GCNGC 4 cut(s) 445, 892, 960, 1327
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 667, 1317
Bme18I GGWCC 2 cut(s) 62, 1107
BmgT120I GGNCC 2 cut(s) 62, 1107
BmiI GGNNCC 4 cut(s) 254, 619, 1059, 1108
BmrFI CCNGG 2 cut(s) 667, 1317
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 4 cut(s) 106, 405, 622, 628
BmuI ACTGGG 1 cut(s) 1199
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 3 cut(s) 308, 546, 1268
BsaAI YACGTR 1 cut(s) 1249
BsaJI CCNNGG 2 cut(s) 718, 1061
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 968
Bse118I RCCGGY 2 cut(s) 402, 851
Bse1I ACTGG 4 cut(s) 287, 879, 1205, 1368
Bse3DI GCAATG 2 cut(s) 444, 902
BseBI CCWGG 2 cut(s) 667, 1317
BseDI CCNNGG 2 cut(s) 718, 1061
BseGI GGATG 1 cut(s) 1262
BseLI CCNNNNNNNGG 2 cut(s) 862, 968
BseMI GCAATG 2 cut(s) 444, 902
BseMII CTCAG 3 cut(s) 351, 808, 1359
BseNI ACTGG 4 cut(s) 287, 879, 1205, 1368
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 874
BseXI GCAGC 4 cut(s) 430, 902, 970, 1337
BshFI GGCC 1 cut(s) 855
BshNI GGYRCC 1 cut(s) 617
BsiHKAI GWGCWC 4 cut(s) 294, 430, 874, 1224
BsiSI CCGG 2 cut(s) 403, 852
BslFI GGGAC 1 cut(s) 1120
BslI CCNNNNNNNGG 2 cut(s) 862, 968
BsmAI GTCTC 2 cut(s) 327, 994
BsmFI GGGAC 1 cut(s) 1120
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 855
Bsp1286I GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
Bsp143I GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
BspANI GGCC 1 cut(s) 855
BspCNI CTCAG 3 cut(s) 350, 807, 1360
BspLI GGNNCC 4 cut(s) 254, 619, 1059, 1108
BspMI ACCTGC 1 cut(s) 1118
BspPI GGATC 1 cut(s) 1043
BspQI GCTCTTC 2 cut(s) 418, 1149
BspT107I GGYRCC 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 444, 902
BsrFI RCCGGY 2 cut(s) 402, 851
BsrI ACTGG 4 cut(s) 287, 879, 1205, 1368
BssAI RCCGGY 2 cut(s) 402, 851
BssECI CCNNGG 2 cut(s) 718, 1061
BssMI GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
BssSI CACGAG 2 cut(s) 815, 963
BssT1I CCWWGG 2 cut(s) 718, 1061
Bst2BI CACGAG 2 cut(s) 815, 963
Bst2UI CCWGG 2 cut(s) 667, 1317
Bst4CI ACNGT 3 cut(s) 358, 487, 1378
Bst6I CTCTTC 2 cut(s) 418, 1149
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstBAI YACGTR 1 cut(s) 1249
BstDEI CTNAG 3 cut(s) 337, 794, 1368
BstF5I GGATG 1 cut(s) 1262
BstKTI GATC 6 cut(s) 69, 592, 600, 814, 1023, 1038
BstMAI GTCTC 2 cut(s) 327, 994
BstMBI GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
BstMWI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
BstNI CCWGG 2 cut(s) 667, 1317
BstSCI CCNGG 2 cut(s) 665, 1315
BstSFI CTRYAG 1 cut(s) 676
BstSLI GKGCMC 2 cut(s) 134, 874
BstV1I GCAGC 4 cut(s) 430, 902, 970, 1337
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 1 cut(s) 855
BtsCI GGATG 1 cut(s) 1262
BtsIMutI CAGTG 2 cut(s) 354, 872
BveI ACCTGC 1 cut(s) 1118
CaiI CAGNNNCTG 1 cut(s) 1211
Cfr10I RCCGGY 2 cut(s) 402, 851
Cfr13I GGNCC 2 cut(s) 62, 1107
CseI GACGC 1 cut(s) 773
CsiI ACCWGGT 1 cut(s) 1315
Csp6I GTAC 7 cut(s) 77, 618, 648, 670, 772, 1201, 1264
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 7 cut(s) 223, 571, 578, 642, 698, 994, 1340
CviQI GTAC 7 cut(s) 77, 618, 648, 670, 772, 1201, 1264
DdeI CTNAG 3 cut(s) 337, 794, 1368
DpnI GATC 6 cut(s) 68, 591, 599, 813, 1022, 1037
DpnII GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
EaeI YGGCCR 1 cut(s) 853
Eam1104I CTCTTC 2 cut(s) 418, 1149
EarI CTCTTC 2 cut(s) 418, 1149
Ecl136II GAGCTC 2 cut(s) 292, 1222
Eco130I CCWWGG 2 cut(s) 718, 1061
Eco24I GRGCYC 2 cut(s) 294, 1224
Eco47I GGWCC 2 cut(s) 62, 1107
Eco53kI GAGCTC 2 cut(s) 292, 1222
EcoICRI GAGCTC 2 cut(s) 292, 1222
EcoO109I RGGNCCY 1 cut(s) 1107
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 665, 1315
EcoT14I CCWWGG 2 cut(s) 718, 1061
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 294, 1224
ErhI CCWWGG 2 cut(s) 718, 1061
FaeI CATG 7 cut(s) 226, 574, 581, 645, 701, 997, 1343
FalI AAGNNNNNCTT 2 cut(s) 1157, 1189
FaqI GGGAC 1 cut(s) 1120
FatI CATG 7 cut(s) 222, 570, 577, 641, 697, 993, 1339
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 4 cut(s) 444, 891, 959, 1326
FokI GGATG 1 cut(s) 1269
FriOI GRGCYC 2 cut(s) 294, 1224
Fsp4HI GCNGC 4 cut(s) 444, 891, 959, 1326
FspBI CTAG 4 cut(s) 270, 512, 719, 1062
GluI GCNGC 4 cut(s) 444, 891, 959, 1326
HaeIII GGCC 1 cut(s) 855
HapII CCGG 2 cut(s) 403, 852
HgaI GACGC 1 cut(s) 773
Hin1II CATG 7 cut(s) 226, 574, 581, 645, 701, 997, 1343
HincII GTYRAC 1 cut(s) 1374
HindII GTYRAC 1 cut(s) 1374
HindIII AAGCTT 3 cut(s) 564, 1231, 1385
HinfI GANTC 7 cut(s) 199, 335, 799, 990, 1066, 1305, 1370
HpaII CCGG 2 cut(s) 403, 852
HphI GGTGA 2 cut(s) 172, 326
Hpy166II GTNNAC 2 cut(s) 872, 1374
Hpy188I TCNGA 4 cut(s) 66, 416, 614, 1032
Hpy188III TCNNGA 4 cut(s) 299, 743, 796, 817
Hpy8I GTNNAC 2 cut(s) 872, 1374
HpyAV CCTTC 6 cut(s) 100, 272, 481, 728, 932, 1247
HpyCH4III ACNGT 3 cut(s) 358, 487, 1378
HpyCH4IV ACGT 3 cut(s) 160, 950, 1248
HpyCH4V TGCA 9 cut(s) 437, 635, 641, 684, 872, 890, 907, 1325, 1355
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
HpyF3I CTNAG 3 cut(s) 337, 794, 1368
HpySE526I ACGT 3 cut(s) 160, 950, 1248
Hsp92II CATG 7 cut(s) 226, 574, 581, 645, 701, 997, 1343
KpnI GGTACC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
LguI GCTCTTC 2 cut(s) 418, 1149
LmnI GCTCC 4 cut(s) 579, 772, 1183, 1219
Lsp1109I GCAGC 4 cut(s) 430, 902, 970, 1337
LweI GCATC 4 cut(s) 106, 405, 622, 628
MabI ACCWGGT 1 cut(s) 1315
MaeI CTAG 4 cut(s) 270, 512, 719, 1062
MaeII ACGT 3 cut(s) 160, 950, 1248
MaeIII GTNAC 5 cut(s) 272, 314, 847, 1046, 1278
MalI GATC 6 cut(s) 68, 591, 599, 813, 1022, 1037
MboI GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
MboII GAAGA 6 cut(s) 125, 435, 514, 561, 926, 1166
MfeI CAATTG 2 cut(s) 593, 803
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
MlyI GAGTC 3 cut(s) 329, 1299, 1379
MmeI TCCRAC 3 cut(s) 340, 366, 906
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 4 cut(s) 308, 725, 1350, 1397
MslI CAYNNNNRTG 1 cut(s) 1344
MspI CCGG 2 cut(s) 403, 852
MspR9I CCNGG 2 cut(s) 667, 1317
MunI CAATTG 2 cut(s) 593, 803
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 2 cut(s) 667, 1317
MwoI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
NlaIII CATG 7 cut(s) 226, 574, 581, 645, 701, 997, 1343
NlaIV GGNNCC 4 cut(s) 254, 619, 1059, 1108
NmuCI GTSAC 2 cut(s) 314, 847
NsiI ATGCAT 1 cut(s) 643
PciSI GCTCTTC 2 cut(s) 418, 1149
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 4 cut(s) 199, 799, 990, 1066
PflMI CCANNNNNTGG 2 cut(s) 862, 968
PkrI GCNGC 4 cut(s) 445, 892, 960, 1327
PleI GAGTC 3 cut(s) 329, 1299, 1378
PpsI GAGTC 3 cut(s) 329, 1299, 1378
Ppu21I YACGTR 1 cut(s) 1249
PpuMI RGGWCCY 1 cut(s) 1107
PshBI ATTAAT 1 cut(s) 1397
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 294, 1224
Psp5II RGGWCCY 1 cut(s) 1107
Psp6I CCWGG 2 cut(s) 665, 1315
PspGI CCWGG 2 cut(s) 665, 1315
PspN4I GGNNCC 4 cut(s) 254, 619, 1059, 1108
PspPI GGNCC 2 cut(s) 62, 1107
PspPPI RGGWCCY 1 cut(s) 1107
PstNI CAGNNNCTG 1 cut(s) 1211
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 7 cut(s) 78, 619, 649, 671, 773, 1202, 1265
RsaNI GTAC 7 cut(s) 77, 618, 648, 670, 772, 1201, 1264
RseI CAYNNNNRTG 1 cut(s) 1344
SacI GAGCTC 2 cut(s) 294, 1224
SapI GCTCTTC 2 cut(s) 418, 1149
SaqAI TTAA 4 cut(s) 308, 725, 1350, 1397
SatI GCNGC 4 cut(s) 444, 891, 959, 1326
Sau3AI GATC 6 cut(s) 66, 589, 597, 811, 1020, 1035
Sau96I GGNCC 2 cut(s) 62, 1107
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 3 cut(s) 329, 1299, 1379
ScrFI CCNGG 2 cut(s) 667, 1317
SduI GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
SexAI ACCWGGT 1 cut(s) 1315
SfaNI GCATC 4 cut(s) 106, 405, 622, 628
SfcI CTRYAG 1 cut(s) 676
SinI GGWCC 2 cut(s) 62, 1107
SmiMI CAYNNNNRTG 1 cut(s) 1344
SmlI CTYRAG 3 cut(s) 287, 561, 1283
SmoI CTYRAG 3 cut(s) 287, 561, 1283
SpeI ACTAGT 1 cut(s) 511
SspI AATATT 1 cut(s) 1194
SspMI CTAG 4 cut(s) 270, 512, 719, 1062
SstI GAGCTC 2 cut(s) 294, 1224
StyD4I CCNGG 2 cut(s) 665, 1315
StyI CCWWGG 2 cut(s) 718, 1061
TaaI ACNGT 3 cut(s) 358, 487, 1378
TaiI ACGT 3 cut(s) 163, 953, 1251
TaqI TCGA 2 cut(s) 15, 657
TatI WGTACW 2 cut(s) 647, 1263
TfiI GAWTC 4 cut(s) 199, 799, 990, 1066
Tru1I TTAA 4 cut(s) 308, 725, 1350, 1397
Tru9I TTAA 4 cut(s) 308, 725, 1350, 1397
TscAI CASTG 2 cut(s) 361, 879
TseFI GTSAC 2 cut(s) 314, 847
TseI GCWGC 4 cut(s) 443, 890, 958, 1325
Tsp45I GTSAC 2 cut(s) 314, 847
TspDTI ATGAA 9 cut(s) 337, 463, 559, 686, 725, 832, 982, 1022, 1225
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 2 cut(s) 361, 879
Van91I CCANNNNNTGG 2 cut(s) 862, 968
VneI GTGCAC 1 cut(s) 870
VpaK11BI GGWCC 2 cut(s) 62, 1107
VspI ATTAAT 1 cut(s) 1397
XapI RAATTY 2 cut(s) 17, 839
XcmI CCANNNNNNNNNTGG 2 cut(s) 863, 1212
XmaJI CCTAGG 2 cut(s) 718, 1061
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 4 cut(s) 270, 512, 719, 1062
ZrmI AGTACT 1 cut(s) 649
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.