Rroxscaffold_2G00137940

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013471
Physical Location & Seq
Forward (+)
75938341 .. 75942027
3687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137940.1

Sequence Viewer

Length: 1386 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGTCTGGCTACATAGCGGAGGGAGAAGATGCTATGATATTGTGCCCAAAGGGAGAGGAGGAAAATTTCAAAAGTAAATGGCTTATATCTTTCACCCTCCTGCTTCAAATGATTCTGTTATGGCTTGCCAAACCCATGAGAAGCTTTGGTTTAGTTTTTAAGTGGTTCCTCAACCTTCCCTCTAGTAACAGAAACTGGCTTGAGCTCTTTCTTGATCTTTTAACAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTCAGATGTCAGATATAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCGTTGGAAGATGGAACTAATCAAGTTCTATGACTTTTATAATTATTATCAAGGAAGACATACTACTCAAGCTTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCGAAGGTTTTCCTGACAAAGAACCAGAACTAGACGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGATACCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACATGCCTCTACTATAACAGCCTGTATAAAGAAAAGATACTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATATTCAAGTATCTAGTTGCTGTTTGGGAGCTCATAAGAAGCTTCATAATTCCACACGTCGAAGGATGGGAGTACAAAGAAACTTGGTTACTCAAGGGGATTAGGGTATTTGGACTCATAATACCAGGTTTTGCAGCTCATAATCCCCATGATTATGTTAATGCAACCCGACTGGCTGAGTCAACTGTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

461

Amino Acids

53.47

Weight (kDa)

8.82

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 201 - 359 9.7e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
Acc65I GGTACC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 617
AccB7I CCANNNNNTGG 2 cut(s) 862, 968
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 1043
AcoI YGGCCR 1 cut(s) 853
AcsI RAATTY 3 cut(s) 17, 151, 839
AfaI GTAC 6 cut(s) 78, 619, 649, 671, 773, 1265
AfiI CCNNNNNNNGG 2 cut(s) 862, 968
AflIII ACRYGT 1 cut(s) 1246
AgsI TTSAA 8 cut(s) 22, 46, 157, 194, 479, 986, 1096, 1198
AjiI CACGTC 1 cut(s) 1249
AjnI CCWGG 2 cut(s) 665, 1315
AluBI AGCT 9 cut(s) 73, 231, 292, 566, 782, 861, 1222, 1233, 1328
AluI AGCT 9 cut(s) 73, 231, 292, 566, 782, 861, 1222, 1233, 1328
Alw21I GWGCWC 4 cut(s) 294, 430, 874, 1224
Alw44I GTGCAC 1 cut(s) 870
AlwI GGATC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 282
AoxI GGCC 1 cut(s) 853
ApaLI GTGCAC 1 cut(s) 870
ApeKI GCWGC 4 cut(s) 443, 890, 958, 1325
ApoI RAATTY 3 cut(s) 17, 151, 839
Asp700I GAANNNNTTC 1 cut(s) 738
Asp718I GGTACC 1 cut(s) 617
AspA2I CCTAGG 2 cut(s) 718, 1061
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 172, 326
AvaII GGWCC 1 cut(s) 62
AvrII CCTAGG 2 cut(s) 718, 1061
BaeGI GKGCMC 2 cut(s) 134, 874
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 294, 1224
BarI GAAGNNNNNNTAC 2 cut(s) 541, 573
BauI CACGAG 2 cut(s) 815, 963
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 4 cut(s) 294, 430, 874, 1224
BbvI GCAGC 4 cut(s) 430, 902, 970, 1337
BccI CCATC 2 cut(s) 499, 1251
BciT130I CCWGG 2 cut(s) 667, 1317
BciVI GTATCC 1 cut(s) 1059
BclI TGATCA 1 cut(s) 589
BfaI CTAG 5 cut(s) 270, 719, 761, 1062, 1205
BfmI CTRYAG 1 cut(s) 676
BfuI GTATCC 1 cut(s) 1059
BglII AGATCT 1 cut(s) 811
BisI GCNGC 4 cut(s) 444, 891, 959, 1326
BlnI CCTAGG 2 cut(s) 718, 1061
BlsI GCNGC 4 cut(s) 445, 892, 960, 1327
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 667, 1317
Bme18I GGWCC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 1249
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 3 cut(s) 254, 619, 1059
BmrFI CCNGG 2 cut(s) 667, 1317
BmsI GCATC 4 cut(s) 106, 405, 622, 628
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 3 cut(s) 308, 546, 1268
BsaJI CCNNGG 2 cut(s) 718, 1061
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 968
Bse118I RCCGGY 2 cut(s) 402, 851
Bse1I ACTGG 3 cut(s) 287, 879, 1368
Bse3DI GCAATG 2 cut(s) 444, 902
BseBI CCWGG 2 cut(s) 667, 1317
BseDI CCNNGG 2 cut(s) 718, 1061
BseGI GGATG 1 cut(s) 1262
BseLI CCNNNNNNNGG 2 cut(s) 862, 968
BseMI GCAATG 2 cut(s) 444, 902
BseMII CTCAG 3 cut(s) 351, 808, 1359
BseNI ACTGG 3 cut(s) 287, 879, 1368
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 874
BseXI GCAGC 4 cut(s) 430, 902, 970, 1337
BshFI GGCC 1 cut(s) 855
BshNI GGYRCC 1 cut(s) 617
BsiHKAI GWGCWC 4 cut(s) 294, 430, 874, 1224
BsiSI CCGG 2 cut(s) 403, 852
BslFI GGGAC 1 cut(s) 1120
BslI CCNNNNNNNGG 2 cut(s) 862, 968
BsmFI GGGAC 1 cut(s) 1120
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 855
Bsp1286I GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
Bsp143I GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 855
BspCNI CTCAG 3 cut(s) 350, 807, 1360
BspLI GGNNCC 3 cut(s) 254, 619, 1059
BspPI GGATC 1 cut(s) 1043
BspQI GCTCTTC 2 cut(s) 418, 1149
BspT107I GGYRCC 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 444, 902
BsrFI RCCGGY 2 cut(s) 402, 851
BsrI ACTGG 3 cut(s) 287, 879, 1368
BssAI RCCGGY 2 cut(s) 402, 851
BssECI CCNNGG 2 cut(s) 718, 1061
BssMI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BssSI CACGAG 2 cut(s) 815, 963
BssT1I CCWWGG 2 cut(s) 718, 1061
Bst2BI CACGAG 2 cut(s) 815, 963
Bst2UI CCWGG 2 cut(s) 667, 1317
Bst4CI ACNGT 3 cut(s) 358, 487, 1378
Bst6I CTCTTC 2 cut(s) 418, 1149
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 3 cut(s) 337, 794, 1368
BstF5I GGATG 1 cut(s) 1262
BstKTI GATC 7 cut(s) 69, 304, 592, 600, 814, 1023, 1038
BstMBI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
BstMWI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
BstNI CCWGG 2 cut(s) 667, 1317
BstNSI RCATGY 1 cut(s) 1113
BstSCI CCNGG 2 cut(s) 665, 1315
BstSFI CTRYAG 1 cut(s) 676
BstSLI GKGCMC 2 cut(s) 134, 874
BstV1I GCAGC 4 cut(s) 430, 902, 970, 1337
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuI GTATCC 1 cut(s) 1059
BsuRI GGCC 1 cut(s) 855
BtrI CACGTC 1 cut(s) 1249
BtsCI GGATG 1 cut(s) 1262
BtsIMutI CAGTG 2 cut(s) 354, 872
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 282
Cfr10I RCCGGY 2 cut(s) 402, 851
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 1 cut(s) 773
CsiI ACCWGGT 1 cut(s) 1315
Csp6I GTAC 6 cut(s) 77, 618, 648, 670, 772, 1264
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 8 cut(s) 223, 571, 578, 642, 698, 994, 1110, 1340
CviQI GTAC 6 cut(s) 77, 618, 648, 670, 772, 1264
DdeI CTNAG 3 cut(s) 337, 794, 1368
DpnI GATC 7 cut(s) 68, 303, 591, 599, 813, 1022, 1037
DpnII GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
EaeI YGGCCR 1 cut(s) 853
Eam1104I CTCTTC 2 cut(s) 418, 1149
EarI CTCTTC 2 cut(s) 418, 1149
Ecl136II GAGCTC 2 cut(s) 292, 1222
Eco130I CCWWGG 2 cut(s) 718, 1061
Eco24I GRGCYC 2 cut(s) 294, 1224
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 2 cut(s) 292, 1222
EcoICRI GAGCTC 2 cut(s) 292, 1222
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 665, 1315
EcoT14I CCWWGG 2 cut(s) 718, 1061
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 294, 1224
ErhI CCWWGG 2 cut(s) 718, 1061
FaeI CATG 8 cut(s) 226, 574, 581, 645, 701, 997, 1113, 1343
FalI AAGNNNNNCTT 2 cut(s) 1157, 1189
FaqI GGGAC 1 cut(s) 1120
FatI CATG 8 cut(s) 222, 570, 577, 641, 697, 993, 1109, 1339
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 4 cut(s) 444, 891, 959, 1326
FokI GGATG 1 cut(s) 1269
FriOI GRGCYC 2 cut(s) 294, 1224
Fsp4HI GCNGC 4 cut(s) 444, 891, 959, 1326
FspBI CTAG 5 cut(s) 270, 719, 761, 1062, 1205
GluI GCNGC 4 cut(s) 444, 891, 959, 1326
HaeIII GGCC 1 cut(s) 855
HapII CCGG 2 cut(s) 403, 852
HgaI GACGC 1 cut(s) 773
Hin1II CATG 8 cut(s) 226, 574, 581, 645, 701, 997, 1113, 1343
HincII GTYRAC 1 cut(s) 1374
HindII GTYRAC 1 cut(s) 1374
HindIII AAGCTT 3 cut(s) 229, 564, 1231
HinfI GANTC 6 cut(s) 199, 335, 799, 990, 1305, 1370
HpaII CCGG 2 cut(s) 403, 852
HphI GGTGA 2 cut(s) 172, 326
Hpy166II GTNNAC 2 cut(s) 872, 1374
Hpy188I TCNGA 5 cut(s) 66, 409, 416, 614, 1032
Hpy188III TCNNGA 4 cut(s) 299, 743, 796, 817
Hpy8I GTNNAC 2 cut(s) 872, 1374
Hpy99I CGWCG 1 cut(s) 1253
HpyAV CCTTC 4 cut(s) 272, 481, 728, 1247
HpyCH4III ACNGT 3 cut(s) 358, 487, 1378
HpyCH4IV ACGT 2 cut(s) 950, 1248
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
HpyF3I CTNAG 3 cut(s) 337, 794, 1368
HpySE526I ACGT 2 cut(s) 950, 1248
Hsp92II CATG 8 cut(s) 226, 574, 581, 645, 701, 997, 1113, 1343
KpnI GGTACC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
LguI GCTCTTC 2 cut(s) 418, 1149
LmnI GCTCC 4 cut(s) 579, 772, 1183, 1219
Lsp1109I GCAGC 4 cut(s) 430, 902, 970, 1337
LweI GCATC 4 cut(s) 106, 405, 622, 628
MabI ACCWGGT 1 cut(s) 1315
MaeI CTAG 5 cut(s) 270, 719, 761, 1062, 1205
MaeII ACGT 2 cut(s) 950, 1248
MaeIII GTNAC 5 cut(s) 272, 314, 847, 1046, 1278
MalI GATC 7 cut(s) 68, 303, 591, 599, 813, 1022, 1037
MboI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
MboII GAAGA 6 cut(s) 125, 435, 514, 561, 926, 1166
MfeI CAATTG 2 cut(s) 593, 803
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
MlyI GAGTC 3 cut(s) 329, 1299, 1379
MmeI TCCRAC 4 cut(s) 340, 366, 479, 906
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 4 cut(s) 246, 308, 725, 1350
MslI CAYNNNNRTG 1 cut(s) 1344
MspI CCGG 2 cut(s) 403, 852
MspR9I CCNGG 2 cut(s) 667, 1317
MunI CAATTG 2 cut(s) 593, 803
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 2 cut(s) 667, 1317
MwoI GCNNNNNNNGC 4 cut(s) 434, 443, 452, 899
NdeI CATATG 1 cut(s) 457
NdeII GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
NlaIII CATG 8 cut(s) 226, 574, 581, 645, 701, 997, 1113, 1343
NlaIV GGNNCC 3 cut(s) 254, 619, 1059
NmuCI GTSAC 2 cut(s) 314, 847
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 1 cut(s) 1113
PciSI GCTCTTC 2 cut(s) 418, 1149
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 199, 799, 990
PflMI CCANNNNNTGG 2 cut(s) 862, 968
PkrI GCNGC 4 cut(s) 445, 892, 960, 1327
PleI GAGTC 3 cut(s) 329, 1299, 1378
PpsI GAGTC 3 cut(s) 329, 1299, 1378
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 294, 1224
Psp6I CCWGG 2 cut(s) 665, 1315
PspGI CCWGG 2 cut(s) 665, 1315
PspN4I GGNNCC 3 cut(s) 254, 619, 1059
PspPI GGNCC 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 6 cut(s) 78, 619, 649, 671, 773, 1265
RsaNI GTAC 6 cut(s) 77, 618, 648, 670, 772, 1264
RseI CAYNNNNRTG 1 cut(s) 1344
SacI GAGCTC 2 cut(s) 294, 1224
SapI GCTCTTC 2 cut(s) 418, 1149
SaqAI TTAA 4 cut(s) 246, 308, 725, 1350
SatI GCNGC 4 cut(s) 444, 891, 959, 1326
Sau3AI GATC 7 cut(s) 66, 301, 589, 597, 811, 1020, 1035
Sau96I GGNCC 1 cut(s) 62
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 3 cut(s) 329, 1299, 1379
ScrFI CCNGG 2 cut(s) 667, 1317
SduI GDGCHC 5 cut(s) 134, 294, 430, 874, 1224
SexAI ACCWGGT 1 cut(s) 1315
SfaNI GCATC 4 cut(s) 106, 405, 622, 628
SfcI CTRYAG 1 cut(s) 676
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 1344
SmlI CTYRAG 3 cut(s) 287, 561, 1283
SmoI CTYRAG 3 cut(s) 287, 561, 1283
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 1194
SspMI CTAG 5 cut(s) 270, 719, 761, 1062, 1205
SstI GAGCTC 2 cut(s) 294, 1224
StyD4I CCNGG 2 cut(s) 665, 1315
StyI CCWWGG 2 cut(s) 718, 1061
TaaI ACNGT 3 cut(s) 358, 487, 1378
TaiI ACGT 2 cut(s) 953, 1251
TaqI TCGA 3 cut(s) 15, 657, 1251
TatI WGTACW 2 cut(s) 647, 1263
TfiI GAWTC 3 cut(s) 199, 799, 990
Tru1I TTAA 4 cut(s) 246, 308, 725, 1350
Tru9I TTAA 4 cut(s) 246, 308, 725, 1350
TscAI CASTG 2 cut(s) 361, 879
TseFI GTSAC 2 cut(s) 314, 847
TseI GCWGC 4 cut(s) 443, 890, 958, 1325
Tsp45I GTSAC 2 cut(s) 314, 847
TspDTI ATGAA 9 cut(s) 337, 463, 559, 686, 725, 832, 982, 1022, 1225
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 2 cut(s) 361, 879
Van91I CCANNNNNTGG 2 cut(s) 862, 968
VneI GTGCAC 1 cut(s) 870
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 3 cut(s) 17, 151, 839
XceI RCATGY 1 cut(s) 1113
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XmaJI CCTAGG 2 cut(s) 718, 1061
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 5 cut(s) 270, 719, 761, 1062, 1205
ZrmI AGTACT 1 cut(s) 649
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.