Rh2BG190400

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013471
Physical Location & Seq
Reverse (-)
17100741 .. 17103777
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG190400.1

Sequence Viewer

Length: 1530 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGGCTGGCTACATAGCGGAGGGAGAAGATGCTATGATATTGTGCCCAAAGGGAGAGGAGGAAAATCTCAAACGTAAATGGCTTATATCTTTCATCCTCCTGCTTCAAATGATTCTGTTATGGATTGCCAAACCCATGAGAAACTTTGGTTTAGTTTTTGAGTGGTTCCTCAACCTTCCTTCTAGTAAGCGAAACTGGCTTGAGCTCTCTCTTGATCTTTTAACAGGTACTCTCTATCTGCATAGACTGCATTTTGTTGCCCTCAATGAAAGTTTCAAAAGCACCTTTTTGTTTTTAAGTTTTTTTATCTATATTTTCAGACCTATATGGTACTTAATTAAAGATGAGTTTAAACTTGCTATACATGCAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTTAGATGTCAGATATAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCATTGGAAGATGAAACTAATCAAGTTCTTTGACTTTTATAATTATTATCAAGGAAGACATACTACTCAAGCCTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGTAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGTGCATTGGGAAAGTTCATGGTGGGTTTATGAAAGCCCTAGGCTTAACCCCCGAAGGTTTTCCTGACAAAGAACCAGAACTAGACGCTCCGTACCCTTTAGCTTACTACACTCTCAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGATACCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACATGCCTCTACTATAACAGCCTGTATAAAGAAAAGATGCTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATATTCAAGTACCTAGTTGCTGCTTGGGAGCTCATAAGAAGCTTCATAATTCCACACGTAGAAGGATGGGAGTACAAAGAAACTTGGTTACTCAAGGGGATTAGGGTATTTGGACTCATAATACCAGGTTTTGCAGCTCATAATCCCCATGATTATGTTAATGCAACCCGACTGGCTGAGTCAACTGTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

509

Amino Acids

59.34

Weight (kDa)

9.03

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 249 - 407 1e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AarI CACCTGC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 446
Acc65I GGTACC 1 cut(s) 761
AccB1I GGYRCC 1 cut(s) 761
AccB7I CCANNNNNTGG 2 cut(s) 1006, 1112
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 1187
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 2 cut(s) 17, 983
AfaI GTAC 9 cut(s) 78, 316, 419, 763, 793, 815, 917, 1346, 1409
AfiI CCNNNNNNNGG 2 cut(s) 1006, 1112
AflIII ACRYGT 1 cut(s) 1390
AgsI TTSAA 8 cut(s) 22, 46, 194, 364, 623, 1130, 1240, 1342
AjnI CCWGG 2 cut(s) 809, 1459
AluBI AGCT 7 cut(s) 73, 292, 926, 1005, 1366, 1377, 1472
AluI AGCT 7 cut(s) 73, 292, 926, 1005, 1366, 1377, 1472
Alw21I GWGCWC 4 cut(s) 294, 574, 1018, 1368
Alw44I GTGCAC 1 cut(s) 1014
AlwI GGATC 1 cut(s) 1187
AoxI GGCC 1 cut(s) 997
ApaLI GTGCAC 1 cut(s) 1014
ApeKI GCWGC 5 cut(s) 587, 1034, 1102, 1355, 1469
ApoI RAATTY 2 cut(s) 17, 983
Asp700I GAANNNNTTC 1 cut(s) 882
Asp718I GGTACC 1 cut(s) 761
AspA2I CCTAGG 2 cut(s) 862, 1205
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 62
AvrII CCTAGG 2 cut(s) 862, 1205
BaeGI GKGCMC 2 cut(s) 134, 1018
BanI GGYRCC 1 cut(s) 761
BanII GRGCYC 2 cut(s) 294, 1368
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BauI CACGAG 2 cut(s) 959, 1107
BbsI GAAGAC 1 cut(s) 700
Bbv12I GWGCWC 4 cut(s) 294, 574, 1018, 1368
BbvI GCAGC 5 cut(s) 574, 1046, 1114, 1342, 1481
BccI CCATC 1 cut(s) 1395
BciT130I CCWGG 2 cut(s) 811, 1461
BciVI GTATCC 1 cut(s) 1203
BclI TGATCA 1 cut(s) 733
BfaI CTAG 5 cut(s) 270, 863, 905, 1206, 1349
BfmI CTRYAG 1 cut(s) 820
BfuAI ACCTGC 1 cut(s) 446
BfuI GTATCC 1 cut(s) 1203
BglII AGATCT 1 cut(s) 955
BisI GCNGC 5 cut(s) 588, 1035, 1103, 1356, 1470
BlnI CCTAGG 2 cut(s) 862, 1205
BlsI GCNGC 5 cut(s) 589, 1036, 1104, 1357, 1471
BmcAI AGTACT 1 cut(s) 793
Bme1390I CCNGG 2 cut(s) 811, 1461
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 3 cut(s) 254, 763, 1203
BmrFI CCNGG 2 cut(s) 811, 1461
BmsI GCATC 4 cut(s) 106, 549, 772, 1278
BpiI GAAGAC 1 cut(s) 700
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpuEI CTTGAG 3 cut(s) 308, 690, 1412
BsaAI YACGTR 1 cut(s) 1393
BsaJI CCNNGG 2 cut(s) 862, 1205
Bsc4I CCNNNNNNNGG 2 cut(s) 1006, 1112
Bse118I RCCGGY 2 cut(s) 546, 995
Bse1I ACTGG 3 cut(s) 287, 1023, 1512
Bse3DI GCAATG 2 cut(s) 588, 1046
BseBI CCWGG 2 cut(s) 811, 1461
BseDI CCNNGG 2 cut(s) 862, 1205
BseGI GGATG 2 cut(s) 180, 1406
BseLI CCNNNNNNNGG 2 cut(s) 1006, 1112
BseMI GCAATG 2 cut(s) 588, 1046
BseMII CTCAG 3 cut(s) 495, 952, 1503
BseNI ACTGG 3 cut(s) 287, 1023, 1512
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 1018
BseXI GCAGC 5 cut(s) 574, 1046, 1114, 1342, 1481
BshFI GGCC 1 cut(s) 999
BshNI GGYRCC 1 cut(s) 761
BsiHKAI GWGCWC 4 cut(s) 294, 574, 1018, 1368
BsiSI CCGG 2 cut(s) 547, 996
BslFI GGGAC 1 cut(s) 1264
BslI CCNNNNNNNGG 2 cut(s) 1006, 1112
BsmFI GGGAC 1 cut(s) 1264
BsmI GAATGC 1 cut(s) 752
BsnI GGCC 1 cut(s) 999
Bsp1286I GDGCHC 5 cut(s) 134, 294, 574, 1018, 1368
Bsp143I GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 999
BspCNI CTCAG 3 cut(s) 494, 951, 1504
BspLI GGNNCC 3 cut(s) 254, 763, 1203
BspMI ACCTGC 1 cut(s) 446
BspPI GGATC 1 cut(s) 1187
BspQI GCTCTTC 2 cut(s) 562, 1293
BspT107I GGYRCC 1 cut(s) 761
BsrDI GCAATG 2 cut(s) 588, 1046
BsrFI RCCGGY 2 cut(s) 546, 995
BsrI ACTGG 3 cut(s) 287, 1023, 1512
BssAI RCCGGY 2 cut(s) 546, 995
BssECI CCNNGG 2 cut(s) 862, 1205
BssMI GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
BssSI CACGAG 2 cut(s) 959, 1107
BssT1I CCWWGG 2 cut(s) 862, 1205
Bst2BI CACGAG 2 cut(s) 959, 1107
Bst2UI CCWGG 2 cut(s) 811, 1461
Bst4CI ACNGT 3 cut(s) 502, 631, 1522
Bst6I CTCTTC 2 cut(s) 562, 1293
BstAPI GCANNNNNTGC 3 cut(s) 334, 578, 587
BstBAI YACGTR 1 cut(s) 1393
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 3 cut(s) 481, 938, 1512
BstF5I GGATG 2 cut(s) 180, 1406
BstKTI GATC 7 cut(s) 69, 304, 736, 744, 958, 1167, 1182
BstMBI GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
BstMWI GCNNNNNNNGC 7 cut(s) 283, 334, 452, 578, 587, 596, 1043
BstNI CCWGG 2 cut(s) 811, 1461
BstNSI RCATGY 2 cut(s) 455, 1257
BstSCI CCNGG 2 cut(s) 809, 1459
BstSFI CTRYAG 1 cut(s) 820
BstSLI GKGCMC 2 cut(s) 134, 1018
BstV1I GCAGC 5 cut(s) 574, 1046, 1114, 1342, 1481
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuI GTATCC 1 cut(s) 1203
BsuRI GGCC 1 cut(s) 999
BtsCI GGATG 2 cut(s) 180, 1406
BtsIMutI CAGTG 2 cut(s) 498, 1016
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 2 cut(s) 546, 995
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 1 cut(s) 917
CsiI ACCWGGT 1 cut(s) 1459
Csp6I GTAC 9 cut(s) 77, 315, 418, 762, 792, 814, 916, 1345, 1408
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 9 cut(s) 223, 452, 715, 722, 786, 842, 1138, 1254, 1484
CviQI GTAC 9 cut(s) 77, 315, 418, 762, 792, 814, 916, 1345, 1408
DdeI CTNAG 3 cut(s) 481, 938, 1512
DpnI GATC 7 cut(s) 68, 303, 735, 743, 957, 1166, 1181
DpnII GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
DraI TTTAAA 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 2 cut(s) 562, 1293
EarI CTCTTC 2 cut(s) 562, 1293
Ecl136II GAGCTC 2 cut(s) 292, 1366
Eco130I CCWWGG 2 cut(s) 862, 1205
Eco24I GRGCYC 2 cut(s) 294, 1368
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 2 cut(s) 292, 1366
EcoICRI GAGCTC 2 cut(s) 292, 1366
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 809, 1459
EcoT14I CCWWGG 2 cut(s) 862, 1205
EcoT22I ATGCAT 1 cut(s) 787
EcoT38I GRGCYC 2 cut(s) 294, 1368
ErhI CCWWGG 2 cut(s) 862, 1205
FaeI CATG 9 cut(s) 226, 455, 718, 725, 789, 845, 1141, 1257, 1487
FalI AAGNNNNNCTT 2 cut(s) 1301, 1333
FaqI GGGAC 1 cut(s) 1264
FatI CATG 9 cut(s) 222, 451, 714, 721, 785, 841, 1137, 1253, 1483
FauNDI CATATG 1 cut(s) 601
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 5 cut(s) 588, 1035, 1103, 1356, 1470
FokI GGATG 2 cut(s) 167, 1413
FriOI GRGCYC 2 cut(s) 294, 1368
Fsp4HI GCNGC 5 cut(s) 588, 1035, 1103, 1356, 1470
FspBI CTAG 5 cut(s) 270, 863, 905, 1206, 1349
GluI GCNGC 5 cut(s) 588, 1035, 1103, 1356, 1470
HaeIII GGCC 1 cut(s) 999
HapII CCGG 2 cut(s) 547, 996
HgaI GACGC 1 cut(s) 917
Hin1II CATG 9 cut(s) 226, 455, 718, 725, 789, 845, 1141, 1257, 1487
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HindIII AAGCTT 1 cut(s) 1375
HinfI GANTC 6 cut(s) 199, 479, 943, 1134, 1449, 1514
HpaII CCGG 2 cut(s) 547, 996
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 2 cut(s) 1016, 1518
Hpy188I TCNGA 5 cut(s) 66, 407, 560, 758, 1176
Hpy188III TCNNGA 4 cut(s) 299, 887, 940, 961
Hpy8I GTNNAC 2 cut(s) 1016, 1518
HpyAV CCTTC 6 cut(s) 272, 276, 625, 721, 872, 1391
HpyCH4III ACNGT 3 cut(s) 502, 631, 1522
HpyCH4IV ACGT 3 cut(s) 160, 1094, 1392
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 334, 452, 578, 587, 596, 1043
HpyF3I CTNAG 3 cut(s) 481, 938, 1512
HpySE526I ACGT 3 cut(s) 160, 1094, 1392
Hsp92II CATG 9 cut(s) 226, 455, 718, 725, 789, 845, 1141, 1257, 1487
KpnI GGTACC 1 cut(s) 765
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
LguI GCTCTTC 2 cut(s) 562, 1293
LmnI GCTCC 4 cut(s) 723, 916, 1327, 1363
Lsp1109I GCAGC 5 cut(s) 574, 1046, 1114, 1342, 1481
LweI GCATC 4 cut(s) 106, 549, 772, 1278
MabI ACCWGGT 1 cut(s) 1459
MaeI CTAG 5 cut(s) 270, 863, 905, 1206, 1349
MaeII ACGT 3 cut(s) 160, 1094, 1392
MaeIII GTNAC 4 cut(s) 458, 991, 1190, 1422
MalI GATC 7 cut(s) 68, 303, 735, 743, 957, 1166, 1181
MboI GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
MboII GAAGA 6 cut(s) 125, 579, 658, 705, 1070, 1310
MfeI CAATTG 2 cut(s) 737, 947
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 5 cut(s) 134, 294, 574, 1018, 1368
MlyI GAGTC 3 cut(s) 473, 1443, 1523
MmeI TCCRAC 3 cut(s) 484, 510, 1050
Mph1103I ATGCAT 1 cut(s) 787
MroXI GAANNNNTTC 1 cut(s) 882
MseI TTAA 7 cut(s) 308, 383, 422, 426, 438, 869, 1494
MslI CAYNNNNRTG 2 cut(s) 456, 1488
MspI CCGG 2 cut(s) 547, 996
MspR9I CCNGG 2 cut(s) 811, 1461
MssI GTTTAAAC 1 cut(s) 439
MunI CAATTG 2 cut(s) 737, 947
Mva1269I GAATGC 1 cut(s) 752
MvaI CCWGG 2 cut(s) 811, 1461
MwoI GCNNNNNNNGC 7 cut(s) 283, 334, 452, 578, 587, 596, 1043
NdeI CATATG 1 cut(s) 601
NdeII GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
NlaIII CATG 9 cut(s) 226, 455, 718, 725, 789, 845, 1141, 1257, 1487
NlaIV GGNNCC 3 cut(s) 254, 763, 1203
NmuCI GTSAC 2 cut(s) 458, 991
NsiI ATGCAT 1 cut(s) 787
NspI RCATGY 2 cut(s) 455, 1257
PacI TTAATTAA 1 cut(s) 426
PaqCI CACCTGC 1 cut(s) 446
PciSI GCTCTTC 2 cut(s) 562, 1293
PctI GAATGC 1 cut(s) 752
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 3 cut(s) 199, 943, 1134
PflMI CCANNNNNTGG 2 cut(s) 1006, 1112
PkrI GCNGC 5 cut(s) 589, 1036, 1104, 1357, 1471
PleI GAGTC 3 cut(s) 473, 1443, 1522
PmeI GTTTAAAC 1 cut(s) 439
PpsI GAGTC 3 cut(s) 473, 1443, 1522
Ppu21I YACGTR 1 cut(s) 1393
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 2 cut(s) 294, 1368
Psp6I CCWGG 2 cut(s) 809, 1459
PspGI CCWGG 2 cut(s) 809, 1459
PspN4I GGNNCC 3 cut(s) 254, 763, 1203
PspPI GGNCC 1 cut(s) 62
PsuI RGATCY 1 cut(s) 955
RsaI GTAC 9 cut(s) 78, 316, 419, 763, 793, 815, 917, 1346, 1409
RsaNI GTAC 9 cut(s) 77, 315, 418, 762, 792, 814, 916, 1345, 1408
RseI CAYNNNNRTG 2 cut(s) 456, 1488
SacI GAGCTC 2 cut(s) 294, 1368
SapI GCTCTTC 2 cut(s) 562, 1293
SaqAI TTAA 7 cut(s) 308, 383, 422, 426, 438, 869, 1494
SatI GCNGC 5 cut(s) 588, 1035, 1103, 1356, 1470
Sau3AI GATC 7 cut(s) 66, 301, 733, 741, 955, 1164, 1179
Sau96I GGNCC 1 cut(s) 62
ScaI AGTACT 1 cut(s) 793
SchI GAGTC 3 cut(s) 473, 1443, 1523
ScrFI CCNGG 2 cut(s) 811, 1461
SduI GDGCHC 5 cut(s) 134, 294, 574, 1018, 1368
SexAI ACCWGGT 1 cut(s) 1459
SfaNI GCATC 4 cut(s) 106, 549, 772, 1278
SfcI CTRYAG 1 cut(s) 820
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 2 cut(s) 456, 1488
SmlI CTYRAG 3 cut(s) 287, 705, 1427
SmoI CTYRAG 3 cut(s) 287, 705, 1427
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 1338
SspMI CTAG 5 cut(s) 270, 863, 905, 1206, 1349
SstI GAGCTC 2 cut(s) 294, 1368
StyD4I CCNGG 2 cut(s) 809, 1459
StyI CCWWGG 2 cut(s) 862, 1205
TaaI ACNGT 3 cut(s) 502, 631, 1522
TaiI ACGT 3 cut(s) 163, 1097, 1395
TaqI TCGA 2 cut(s) 15, 801
TatI WGTACW 2 cut(s) 791, 1407
TfiI GAWTC 3 cut(s) 199, 943, 1134
Tru1I TTAA 7 cut(s) 308, 383, 422, 426, 438, 869, 1494
Tru9I TTAA 7 cut(s) 308, 383, 422, 426, 438, 869, 1494
TscAI CASTG 2 cut(s) 505, 1023
TseFI GTSAC 2 cut(s) 458, 991
TseI GCWGC 5 cut(s) 587, 1034, 1102, 1355, 1469
Tsp45I GTSAC 2 cut(s) 458, 991
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 2 cut(s) 505, 1023
Van91I CCANNNNNTGG 2 cut(s) 1006, 1112
VneI GTGCAC 1 cut(s) 1014
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 2 cut(s) 17, 983
XceI RCATGY 2 cut(s) 455, 1257
XcmI CCANNNNNNNNNTGG 1 cut(s) 1007
XmaJI CCTAGG 2 cut(s) 862, 1205
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 5 cut(s) 270, 863, 905, 1206, 1349
ZrmI AGTACT 1 cut(s) 793
Zsp2I ATGCAT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.