AT1G50630

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004011
Physical Location & Seq
Reverse (-)
18751456 .. 18753941
2486 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50630.1

Sequence Viewer

Length: 1362 bp
ATGGCGGACATCGACATCGCCGTCGGGAAAGAGCCGCTGATAAACCATGAGAACAACAACAACACGAACAAGATCTTCAATCGCTGCGTTTCACACCAACAAGACGAGCTACACAGCTTCCGCAAATACCTAAGGTGGATGTGTGTAGATCACTCTAGTCCATGGACAGCGATTCTCTCTTGGACCATGTTCATCGTCTTCACACTCGTCGTTCCGGCGATCTCTCACTTCCTCCTCGCTTGTGCTGATTGCGACAGTTACCATTCAAGACCTTACGACTCCGTCGTTCAGCTTTCTCTAAGCAGCGTCGCCACCGTCTCTTTCCTCTGTCTCACCAGATTCGTTAGCAAGTATGGTCTTCGACGCTTCCTCTTCTTTGATAAACTCTGGGACGAGAGCGAAACCGTTCGACGAAATTATACCAATCAACTCAATACTTCGCTTCATATTGTGTCCTACTTTGTGATTCCTTGTTTCTCAGCAATGAGTGCATACAAAATTTGGTGGTACGCTTCAGGTGGATCTCGAATACCTTTTTTAGGAAATGCTGTCTTAAGCGATACAGTGGCTTGTATAATGGAGCTTTGTTCTTGGCTATACCGAACAACCGTAATCTTCCTTGTTTGTGTTCTCTTCCGTCTCATTTGCCATCTTCAGATCCTTCGTCTTCAAGACTTTGCTAAGCTCTTTCAGATTGATTCAGATGTTGGTTCTATCTTGTCTGAACATCTTCGTATCAGACGTCATCTTAGAATCATTAGCCATAGATATCGTTCTTTCATTCTATGTTTATTGATTCTAGTTACTGGAAGTCAGTTTTCTTCTCTGCTTATTACTACCAAAGCCTACACTGAAGTCAATATCTACAGAGCTGGAGAACTCGCTCTTTGTTCTATGACATTAGTTACTGCACTTCTGATACTATTACGAAGTGCATCAAAGATCACACACAAGGCTCAGGCTGTGACATGTTTAGCTGCGAAATGGCATGTATGCGCCACGTTAGAATCCTTTGATCAGACAGTAGAATCCTTTGATCAGACAGTAGAGACTCCAACTTTGGTTGCTAGAAACAACAATGATAACAACAATGTCCATGATGTTGTTACGTTGACAGAATCAGACAGTGACGAGTATGGTGATGAAGAAGATGATCTAGACAATAATGACATCATTCCTGTTTATGCTTTCAGTACCATGTCATTCCAAAAACGGCAAGCTCTAGTGAGTTATTTCGAGAATAACAGCGCAGGAATCACAGTGTATGGGTTTACACTTGATAGAGGTACTCTCCATACAATCTTCGGACTTGAATTGTCTCTTGTTCTCTGGCTTCTTGGTAAAACCATTGGAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

453

Amino Acids

51.6

Weight (kDa)

6.2

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 35 - 437 1.2e-173 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
AatII GACGTC 1 cut(s) 745
AciI CCGC 3 cut(s) 5, 35, 121
AclWI GGATC 2 cut(s) 529, 652
AcsI RAATTY 2 cut(s) 498, 1353
AcuI CTGAAG 3 cut(s) 498, 638, 873
AcyI GRCGYC 1 cut(s) 742
AfaI GTAC 3 cut(s) 509, 1195, 1288
AfiI CCNNNNNNNGG 1 cut(s) 539
AflII CTTAAG 1 cut(s) 553
AflIII ACRYGT 1 cut(s) 968
AgsI TTSAA 4 cut(s) 79, 267, 671, 1313
AluBI AGCT 8 cut(s) 109, 117, 292, 583, 685, 872, 977, 1220
AluI AGCT 8 cut(s) 109, 117, 292, 583, 685, 872, 977, 1220
Alw26I GTCTC 5 cut(s) 322, 335, 644, 1043, 1323
AlwI GGATC 2 cut(s) 529, 652
ApeKI GCWGC 3 cut(s) 84, 303, 977
ApoI RAATTY 2 cut(s) 498, 1353
ArsI GACNNNNNNTTYG 2 cut(s) 1042, 1074
Asp700I GAANNNNTTC 2 cut(s) 405, 729
AspLEI GCGC 2 cut(s) 998, 1250
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 2 cut(s) 325, 1151
AvaII GGWCC 1 cut(s) 183
AxyI CCTNAGG 1 cut(s) 131
BbsI GAAGAC 3 cut(s) 190, 350, 659
BbvI GCAGC 3 cut(s) 71, 315, 964
BccI CCATC 1 cut(s) 657
BceAI ACGGC 2 cut(s) 5, 1229
BclI TGATCA 2 cut(s) 1015, 1036
BcoDI GTCTC 5 cut(s) 322, 335, 644, 1043, 1323
BfaI CTAG 5 cut(s) 156, 800, 1068, 1157, 1223
BfmI CTRYAG 1 cut(s) 865
BfrI CTTAAG 1 cut(s) 553
BglII AGATCT 1 cut(s) 72
BisI GCNGC 4 cut(s) 35, 85, 304, 978
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 4 cut(s) 36, 86, 305, 979
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 1 cut(s) 183
BmsI GCATC 1 cut(s) 944
BpiI GAAGAC 3 cut(s) 190, 350, 659
BplI GAGNNNNNCTC 2 cut(s) 1275, 1307
BpmI CTGGAG 1 cut(s) 894
Bpu10I CCTNAGC 1 cut(s) 957
Bpu1102I GCTNAGC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 742
BsaJI CCNNGG 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 811
Bse21I CCTNAGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 2 cut(s) 492, 971
BseNI ACTGG 1 cut(s) 811
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 71, 315, 964
BsgI GTGCAG 1 cut(s) 894
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 5 cut(s) 322, 335, 644, 1043, 1323
BsmBI CGTCTC 2 cut(s) 322, 644
BsmFI GGGAC 1 cut(s) 404
Bsp143I GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
Bsp1720I GCTNAGC 1 cut(s) 681
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 3 cut(s) 5, 35, 121
BspCNI CTCAG 2 cut(s) 491, 970
BspPI GGATC 2 cut(s) 529, 652
BspTI CTTAAG 1 cut(s) 553
BsrDI GCAATG 1 cut(s) 489
BsrI ACTGG 1 cut(s) 811
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
BssNI GRCGYC 1 cut(s) 742
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 9 cut(s) 257, 316, 406, 565, 610, 1024, 1045, 1127, 1261
Bst6I CTCTTC 2 cut(s) 377, 638
BstACI GRCGYC 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 553
BstAPI GCANNNNNTGC 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 1218
BstDEI CTNAG 6 cut(s) 131, 299, 478, 681, 749, 957
BstDSI CCRYGG 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 144
BstHHI GCGC 2 cut(s) 998, 1250
BstKTI GATC 9 cut(s) 75, 151, 222, 524, 660, 945, 1018, 1039, 1156
BstMAI GTCTC 5 cut(s) 322, 335, 644, 1043, 1323
BstMBI GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNSI RCATGY 2 cut(s) 972, 992
BstSFI CTRYAG 1 cut(s) 865
BstV1I GCAGC 3 cut(s) 71, 315, 964
BstV2I GAAGAC 3 cut(s) 190, 350, 659
BstX2I RGATCY 3 cut(s) 72, 521, 657
BstYI RGATCY 3 cut(s) 72, 521, 657
Bsu36I CCTNAGG 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 144
BtsIMutI CAGTG 4 cut(s) 570, 849, 1132, 1266
Cac8I GCNNGC 1 cut(s) 1218
CfoI GCGC 2 cut(s) 998, 1250
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 2 cut(s) 295, 372
Csp6I GTAC 3 cut(s) 508, 1194, 1287
CviAII CATG 7 cut(s) 47, 162, 187, 969, 989, 1097, 1198
CviQI GTAC 3 cut(s) 508, 1194, 1287
DdeI CTNAG 6 cut(s) 131, 299, 478, 681, 749, 957
DpnI GATC 9 cut(s) 74, 150, 221, 523, 659, 944, 1017, 1038, 1155
DpnII GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
Eam1104I CTCTTC 2 cut(s) 377, 638
EarI CTCTTC 2 cut(s) 377, 638
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 161
Eco32I GATATC 1 cut(s) 770
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 3 cut(s) 498, 638, 873
Eco81I CCTNAGG 1 cut(s) 131
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 770
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 2 cut(s) 322, 644
FaeI CATG 7 cut(s) 50, 165, 190, 972, 992, 1100, 1201
FaqI GGGAC 1 cut(s) 404
FatI CATG 7 cut(s) 46, 161, 186, 968, 988, 1096, 1197
FbaI TGATCA 2 cut(s) 1015, 1036
Fnu4HI GCNGC 4 cut(s) 35, 85, 304, 978
FokI GGATG 1 cut(s) 151
Fsp4HI GCNGC 4 cut(s) 35, 85, 304, 978
FspBI CTAG 5 cut(s) 156, 800, 1068, 1157, 1223
GlaI GCGC 2 cut(s) 997, 1249
GluI GCNGC 4 cut(s) 35, 85, 304, 978
GsuI CTGGAG 1 cut(s) 894
HapII CCGG 1 cut(s) 215
HgaI GACGC 2 cut(s) 295, 372
HhaI GCGC 2 cut(s) 998, 1250
Hin1I GRCGYC 1 cut(s) 742
Hin1II CATG 7 cut(s) 50, 165, 190, 972, 992, 1100, 1201
Hin6I GCGC 2 cut(s) 996, 1248
HinP1I GCGC 2 cut(s) 996, 1248
HincII GTYRAC 1 cut(s) 1113
HindII GTYRAC 1 cut(s) 1113
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 325, 1151
Hpy166II GTNNAC 2 cut(s) 1113, 1272
Hpy188III TCNNGA 7 cut(s) 25, 267, 525, 671, 1157, 1237, 1359
Hpy8I GTNNAC 2 cut(s) 1113, 1272
Hpy99I CGWCG 6 cut(s) 26, 212, 287, 311, 366, 414
HpyAV CCTTC 1 cut(s) 671
HpyCH4III ACNGT 9 cut(s) 257, 316, 406, 565, 610, 1024, 1045, 1127, 1261
HpyCH4IV ACGT 3 cut(s) 742, 1001, 1109
HpyCH4V TGCA 3 cut(s) 491, 911, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 6 cut(s) 131, 299, 478, 681, 749, 957
HpySE526I ACGT 3 cut(s) 742, 1001, 1109
Hsp92I GRCGYC 1 cut(s) 742
Hsp92II CATG 7 cut(s) 50, 165, 190, 972, 992, 1100, 1201
HspAI GCGC 2 cut(s) 996, 1248
Ksp22I TGATCA 2 cut(s) 1015, 1036
Kzo9I GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 3 cut(s) 71, 315, 964
LweI GCATC 1 cut(s) 944
MaeI CTAG 5 cut(s) 156, 800, 1068, 1157, 1223
MaeII ACGT 3 cut(s) 742, 1001, 1109
MaeIII GTNAC 6 cut(s) 257, 802, 904, 964, 1105, 1127
MalI GATC 9 cut(s) 74, 150, 221, 523, 659, 944, 1017, 1038, 1155
MboI GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
MflI RGATCY 3 cut(s) 72, 521, 657
MluCI AATT 4 cut(s) 415, 498, 1313, 1353
MlyI GAGTC 2 cut(s) 272, 1045
MmeI TCCRAC 1 cut(s) 1079
MnlI CCTC 5 cut(s) 242, 245, 335, 380, 1277
MroXI GAANNNNTTC 2 cut(s) 405, 729
MseI TTAA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 37
MspCI CTTAAG 1 cut(s) 553
MspI CCGG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 488
NcoI CCATGG 1 cut(s) 161
NdeII GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
NlaIII CATG 7 cut(s) 50, 165, 190, 972, 992, 1100, 1201
NmuCI GTSAC 2 cut(s) 964, 1127
NspI RCATGY 2 cut(s) 972, 992
PciI ACATGT 1 cut(s) 968
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 282, 739
PdmI GAANNNNTTC 2 cut(s) 405, 729
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 4 cut(s) 36, 86, 305, 979
PleI GAGTC 2 cut(s) 272, 1045
PpsI GAGTC 2 cut(s) 272, 1045
PscI ACATGT 1 cut(s) 968
PspPI GGNCC 1 cut(s) 183
PsuI RGATCY 3 cut(s) 72, 521, 657
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 3 cut(s) 509, 1195, 1288
RsaNI GTAC 3 cut(s) 508, 1194, 1287
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 4 cut(s) 35, 85, 304, 978
Sau3AI GATC 9 cut(s) 72, 148, 219, 521, 657, 942, 1015, 1036, 1153
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 2 cut(s) 272, 1045
SfaNI GCATC 1 cut(s) 944
SfcI CTRYAG 1 cut(s) 865
SinI GGWCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
Sse9I AATT 4 cut(s) 415, 498, 1313, 1353
SsiI CCGC 3 cut(s) 5, 35, 121
SspMI CTAG 5 cut(s) 156, 800, 1068, 1157, 1223
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 9 cut(s) 257, 316, 406, 565, 610, 1024, 1045, 1127, 1261
TaiI ACGT 3 cut(s) 745, 1004, 1112
TaqI TCGA 5 cut(s) 12, 361, 409, 526, 1236
TasI AATT 4 cut(s) 415, 498, 1313, 1353
TauI GCSGC 1 cut(s) 37
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 4 cut(s) 570, 856, 1132, 1266
TseFI GTSAC 2 cut(s) 964, 1127
TseI GCWGC 3 cut(s) 84, 303, 977
Tsp45I GTSAC 2 cut(s) 964, 1127
TspDTI ATGAA 4 cut(s) 181, 434, 769, 1158
TspGWI ACGGA 2 cut(s) 271, 626
TspRI CASTG 4 cut(s) 570, 856, 1132, 1266
Tth111I GACNNNGTC 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 2 cut(s) 498, 1353
XbaI TCTAGA 1 cut(s) 1156
XceI RCATGY 2 cut(s) 972, 992
XmnI GAANNNNTTC 2 cut(s) 405, 729
XspI CTAG 5 cut(s) 156, 800, 1068, 1157, 1223
ZraI GACGTC 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.