MD11G1210400.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004011
Physical Location & Seq
Reverse (-)
30777150 .. 30779773
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1210400.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGAGAAACGATGTCATGGAGGGCTTGTTAAACCAGAACAACAAGACAAACACAGGGTTAGGACGCTGCGTTTCACACATACAAGATGAATTAAAGAGTTTTAGATCGTATGTACGATGGATGTGTGTGGACCAATCTAATGCATGGAGGGCATGCATTTCTTGGTCCATATTCATCTTGTTTGCAATCGTTGTTCCTGCCATTTCCCATTTTGTGTTCACATGTGCATCATGCGATGGCAAGCACAAGAGGCCTTATGATAGTGTAGTGCAATTGTCTTTGAGCAGCGTTGCGACGGTGTCGTTTTTGTGCCTGTCATGGTTTGTGAGGAAGTTTGGGCTCCGGAGGTTCTTGTTTTTCGATAAGCTTTATAATGAGAGCGAGACGGTCAGGAGGGAATACACACAACAGCTCAATAGATCATTGAAGATTCTCTGCATCTTTGTAATGCCATGCTTTGCCGCGGAGAGTGCATATAAGATTTGGTGGTACGCATCTAGAGCCTCGCAAATGCCTTTTCTGGGCAATGTTTACGTGAGTGACGTGGTAGCATGCACGCTAGAGTTGTGCTCGTGGTTGTATCGTACAACTGTGTTTTTTCTCGTGTGCGTTCTGTTCCGTCTAATATGCTACCTCCAAATCCTACGGCTCCAAGACTTTGCCACTGTGTTCCAAGAGCAATCTGATGTCGGGTCAGTGTTGTCCGAGCACCTTAGGATCCGAAGGCACTTAACAATCATAAGTCATAGATACCGAGGTTTCATCCTATGGTCATTGATCTTGGTCACGGGCAGCCAATTTTCCTTGTTGCTCATCACAACCAGGTCTAGTGCCACTGCCAATCTTGATGTTTATAGATCCGGTGAACTTGCGTTATGCTCCATAACTTTAGTCACTGGAGTCCTCATCTTACTGCGCAGTGCAACCAAAATCACACACAAGGCCCAAGCAGTCACATGCCTTGCTACAAAGTGGCACGTTTGCGCCACATTGGAATCCTTTGATGCGAACGAAGGAGATTCTCCGCCGCCTGATGAGACGGGGAATGGCCGAGTGGTTTCTGGTGCCAGTAGTGACAGAGAATCAGACAGTGATTTGGCAAGTGATGATCAAGATGAACTGGAAAACGCCAAGTTTGTTCCATCATATGCATATAGTACAATCTCTTATCAAAAGAGACAAGCTCTAGTGACATATTTGGAAAATAATAGAGCTGGTATTACTATATATGGATTTACATTGGACAGGACTACACTCCACACCATATTTATGCTTGAGCTATCATTGATTCTTTGGTTGTTGGGTAAAACAGTTGGAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

442

Amino Acids

50.08

Weight (kDa)

8.44

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 29 - 425 2.4e-173 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
Acc16I TGCGCA 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 1064
AccII CGCG 1 cut(s) 464
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 4 cut(s) 462, 464, 1025, 1028
AclWI GGATC 3 cut(s) 712, 725, 852
AcoI YGGCCR 1 cut(s) 1048
AcsI RAATTY 1 cut(s) 1317
AfaI GTAC 4 cut(s) 114, 491, 586, 1159
AfiI CCNNNNNNNGG 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 221
AgsI TTSAA 1 cut(s) 427
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 821
AluBI AGCT 5 cut(s) 367, 412, 1184, 1214, 1279
AluI AGCT 5 cut(s) 367, 412, 1184, 1214, 1279
Alw21I GWGCWC 2 cut(s) 572, 711
Alw26I GTCTC 3 cut(s) 377, 1031, 1171
AlwI GGATC 3 cut(s) 712, 725, 852
Aor13HI TCCGGA 1 cut(s) 342
AoxI GGCC 3 cut(s) 251, 942, 1048
ApeKI GCWGC 3 cut(s) 66, 285, 792
ApoI RAATTY 1 cut(s) 1317
AspLEI GCGC 2 cut(s) 918, 986
AspS9I GGNCC 3 cut(s) 130, 165, 943
AsuHPI GGTGA 1 cut(s) 875
AvaII GGWCC 2 cut(s) 130, 165
AxyI CCTNAGG 1 cut(s) 713
BaeI ACNNNNGTAYC 2 cut(s) 742, 775
BamHI GGATCC 1 cut(s) 717
BanI GGYRCC 1 cut(s) 1064
BanII GRGCYC 1 cut(s) 342
BauI CACGAG 2 cut(s) 571, 602
Bbv12I GWGCWC 2 cut(s) 572, 711
BbvI GCAGC 3 cut(s) 53, 297, 804
BccI CCATC 3 cut(s) 111, 230, 1150
BceAI ACGGC 1 cut(s) 662
BciT130I CCWGG 1 cut(s) 823
BclI TGATCA 1 cut(s) 1108
BcoDI GTCTC 3 cut(s) 377, 1031, 1171
BfaI CTAG 4 cut(s) 498, 560, 828, 1187
BisI GCNGC 5 cut(s) 67, 286, 462, 793, 1028
BlsI GCNGC 5 cut(s) 68, 287, 463, 794, 1029
Bme1390I CCNGG 1 cut(s) 823
Bme18I GGWCC 2 cut(s) 130, 165
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 130, 165, 943
BmiI GGNNCC 4 cut(s) 341, 650, 719, 1066
BmrFI CCNGG 1 cut(s) 823
BmsI GCATC 4 cut(s) 236, 447, 503, 994
BplI GAGNNNNNCTC 4 cut(s) 554, 586, 1168, 1200
BpmI CTGGAG 1 cut(s) 918
BpuEI CTTGAG 1 cut(s) 1295
BsaAI YACGTR 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 462, 754
BsaWI WCCGGW 2 cut(s) 342, 860
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse1I ACTGG 3 cut(s) 901, 1068, 1125
Bse21I CCTNAGG 1 cut(s) 713
Bse3DI GCAATG 1 cut(s) 532
BseAI TCCGGA 1 cut(s) 342
BseBI CCWGG 1 cut(s) 823
BseDI CCNNGG 2 cut(s) 462, 754
BseGI GGATG 2 cut(s) 126, 762
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMI GCAATG 1 cut(s) 532
BseNI ACTGG 3 cut(s) 901, 1068, 1125
BseXI GCAGC 3 cut(s) 53, 297, 804
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 3 cut(s) 253, 944, 1050
BshNI GGYRCC 1 cut(s) 1064
BsiHKAI GWGCWC 2 cut(s) 572, 711
BsiSI CCGG 2 cut(s) 343, 861
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 3 cut(s) 377, 1031, 1171
BsmBI CGTCTC 2 cut(s) 377, 1031
BsnI GGCC 3 cut(s) 253, 944, 1050
Bsp1286I GDGCHC 3 cut(s) 342, 572, 711
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
BspACI CCGC 4 cut(s) 462, 464, 1025, 1028
BspANI GGCC 3 cut(s) 253, 944, 1050
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 4 cut(s) 341, 650, 719, 1066
BspPI GGATC 3 cut(s) 712, 725, 852
BspT107I GGYRCC 1 cut(s) 1064
BsrDI GCAATG 1 cut(s) 532
BsrI ACTGG 3 cut(s) 901, 1068, 1125
BssECI CCNNGG 2 cut(s) 462, 754
BssMI GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
BssSI CACGAG 2 cut(s) 571, 602
Bst2BI CACGAG 2 cut(s) 571, 602
Bst2UI CCWGG 1 cut(s) 823
Bst4CI ACNGT 6 cut(s) 298, 388, 592, 667, 1091, 1312
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 154, 242, 553, 557
BstDEI CTNAG 1 cut(s) 713
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 126, 762
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 2 cut(s) 918, 986
BstKTI GATC 6 cut(s) 107, 422, 720, 780, 860, 1111
BstMAI GTCTC 3 cut(s) 377, 1031, 1171
BstMBI GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 149, 250, 470, 500
BstNI CCWGG 1 cut(s) 823
BstNSI RCATGY 4 cut(s) 156, 225, 555, 960
BstSCI CCNGG 1 cut(s) 821
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 3 cut(s) 53, 297, 804
BstX2I RGATCY 2 cut(s) 717, 857
BstYI RGATCY 2 cut(s) 717, 857
Bsu36I CCTNAGG 1 cut(s) 713
BsuRI GGCC 3 cut(s) 253, 944, 1050
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 2 cut(s) 126, 762
BtsI GCAGTG 2 cut(s) 834, 925
BtsIMutI CAGTG 6 cut(s) 663, 702, 834, 894, 925, 1096
Cac8I GCNNGC 4 cut(s) 154, 242, 553, 557
CfoI GCGC 2 cut(s) 918, 986
Cfr13I GGNCC 3 cut(s) 130, 165, 943
Cfr42I CCGCGG 1 cut(s) 465
CseI GACGC 1 cut(s) 72
CsiI ACCWGGT 1 cut(s) 821
Csp6I GTAC 4 cut(s) 113, 490, 585, 1158
CviAII CATG 9 cut(s) 16, 144, 153, 222, 231, 318, 453, 552, 957
CviQI GTAC 4 cut(s) 113, 490, 585, 1158
DdeI CTNAG 1 cut(s) 713
DpnI GATC 6 cut(s) 106, 421, 719, 779, 859, 1110
DpnII GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
EaeI YGGCCR 1 cut(s) 1048
EciI GGCGGA 1 cut(s) 1014
Eco147I AGGCCT 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 130, 165
Eco81I CCTNAGG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 821
EcoT22I ATGCAT 3 cut(s) 145, 158, 1153
EcoT38I GRGCYC 1 cut(s) 342
Esp3I CGTCTC 2 cut(s) 377, 1031
FaeI CATG 9 cut(s) 19, 147, 156, 225, 234, 321, 456, 555, 960
FatI CATG 9 cut(s) 15, 143, 152, 221, 230, 317, 452, 551, 956
FauNDI CATATG 1 cut(s) 1147
FbaI TGATCA 1 cut(s) 1108
Fnu4HI GCNGC 5 cut(s) 67, 286, 462, 793, 1028
FokI GGATG 2 cut(s) 133, 749
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 67, 286, 462, 793, 1028
FspBI CTAG 4 cut(s) 498, 560, 828, 1187
FspI TGCGCA 1 cut(s) 917
GlaI GCGC 2 cut(s) 917, 985
GluI GCNGC 5 cut(s) 67, 286, 462, 793, 1028
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 3 cut(s) 253, 944, 1050
HapII CCGG 2 cut(s) 343, 861
HgaI GACGC 1 cut(s) 72
HhaI GCGC 2 cut(s) 918, 986
Hin1II CATG 9 cut(s) 19, 147, 156, 225, 234, 321, 456, 555, 960
Hin6I GCGC 2 cut(s) 916, 984
HinP1I GCGC 2 cut(s) 916, 984
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 6 cut(s) 430, 900, 995, 1019, 1082, 1288
HpaII CCGG 2 cut(s) 343, 861
HphI GGTGA 1 cut(s) 875
Hpy166II GTNNAC 4 cut(s) 130, 219, 532, 866
Hpy188I TCNGA 4 cut(s) 685, 706, 722, 1087
Hpy188III TCNNGA 6 cut(s) 343, 391, 498, 845, 1112, 1323
Hpy8I GTNNAC 4 cut(s) 130, 219, 532, 866
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 2 cut(s) 717, 1007
HpyCH4III ACNGT 6 cut(s) 298, 388, 592, 667, 1091, 1312
HpyCH4IV ACGT 3 cut(s) 534, 543, 978
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 250, 470, 500
HpyF3I CTNAG 1 cut(s) 713
HpySE526I ACGT 3 cut(s) 534, 543, 978
Hsp92II CATG 9 cut(s) 19, 147, 156, 225, 234, 321, 456, 555, 960
HspAI GCGC 2 cut(s) 916, 984
Kpn2I TCCGGA 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 1108
KspI CCGCGG 1 cut(s) 465
Kzo9I GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
LmnI GCTCC 3 cut(s) 345, 654, 884
Lsp1109I GCAGC 3 cut(s) 53, 297, 804
LweI GCATC 4 cut(s) 236, 447, 503, 994
MabI ACCWGGT 1 cut(s) 821
MaeI CTAG 4 cut(s) 498, 560, 828, 1187
MaeII ACGT 3 cut(s) 534, 543, 978
MaeIII GTNAC 6 cut(s) 539, 784, 892, 952, 1073, 1189
MalI GATC 6 cut(s) 106, 421, 719, 779, 859, 1110
MboI GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
MboII GAAGA 1 cut(s) 439
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 2 cut(s) 717, 857
MhlI GDGCHC 3 cut(s) 342, 572, 711
MluCI AATT 4 cut(s) 89, 272, 797, 1317
MlyI GAGTC 1 cut(s) 909
MmeI TCCRAC 1 cut(s) 1294
Mph1103I ATGCAT 3 cut(s) 145, 158, 1153
MroI TCCGGA 1 cut(s) 342
MseI TTAA 3 cut(s) 29, 92, 731
MslI CAYNNNNRTG 1 cut(s) 1268
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 343, 861
MspR9I CCNGG 1 cut(s) 823
MunI CAATTG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 823
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 149, 250, 470, 500
NdeI CATATG 1 cut(s) 1147
NdeII GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
NlaIII CATG 9 cut(s) 19, 147, 156, 225, 234, 321, 456, 555, 960
NlaIV GGNNCC 4 cut(s) 341, 650, 719, 1066
NmeAIII GCCGAG 1 cut(s) 1076
NmuCI GTSAC 6 cut(s) 539, 784, 892, 952, 1073, 1189
NsbI TGCGCA 1 cut(s) 917
NsiI ATGCAT 3 cut(s) 145, 158, 1153
NspI RCATGY 4 cut(s) 156, 225, 555, 960
PaeI GCATGC 2 cut(s) 156, 555
PceI AGGCCT 1 cut(s) 253
PciI ACATGT 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 5 cut(s) 430, 995, 1019, 1082, 1288
PflFI GACNNNGTC 1 cut(s) 298
PkrI GCNGC 5 cut(s) 68, 287, 463, 794, 1029
PleI GAGTC 1 cut(s) 908
PpsI GAGTC 1 cut(s) 908
Ppu21I YACGTR 1 cut(s) 535
PscI ACATGT 1 cut(s) 221
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 821
PspGI CCWGG 1 cut(s) 821
PspN4I GGNNCC 4 cut(s) 341, 650, 719, 1066
PspPI GGNCC 3 cut(s) 130, 165, 943
PsuI RGATCY 2 cut(s) 717, 857
PsyI GACNNNGTC 1 cut(s) 298
RsaI GTAC 4 cut(s) 114, 491, 586, 1159
RsaNI GTAC 4 cut(s) 113, 490, 585, 1158
RseI CAYNNNNRTG 1 cut(s) 1268
SacII CCGCGG 1 cut(s) 465
SaqAI TTAA 3 cut(s) 29, 92, 731
SatI GCNGC 5 cut(s) 67, 286, 462, 793, 1028
Sau3AI GATC 6 cut(s) 104, 419, 717, 777, 857, 1108
Sau96I GGNCC 3 cut(s) 130, 165, 943
SchI GAGTC 1 cut(s) 909
ScrFI CCNGG 1 cut(s) 823
SduI GDGCHC 3 cut(s) 342, 572, 711
SexAI ACCWGGT 1 cut(s) 821
SfaNI GCATC 4 cut(s) 236, 447, 503, 994
Sfr303I CCGCGG 1 cut(s) 465
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 2 cut(s) 130, 165
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 1 cut(s) 1274
SmoI CTYRAG 1 cut(s) 1274
SphI GCATGC 2 cut(s) 156, 555
Sse9I AATT 4 cut(s) 89, 272, 797, 1317
SseBI AGGCCT 1 cut(s) 253
SsiI CCGC 4 cut(s) 462, 464, 1025, 1028
SspMI CTAG 4 cut(s) 498, 560, 828, 1187
StuI AGGCCT 1 cut(s) 253
StyD4I CCNGG 1 cut(s) 821
TaaI ACNGT 6 cut(s) 298, 388, 592, 667, 1091, 1312
TaiI ACGT 3 cut(s) 537, 546, 981
TaqI TCGA 1 cut(s) 360
TasI AATT 4 cut(s) 89, 272, 797, 1317
TatI WGTACW 1 cut(s) 1157
TauI GCSGC 2 cut(s) 464, 1030
TfiI GAWTC 5 cut(s) 430, 995, 1019, 1082, 1288
Tru1I TTAA 3 cut(s) 29, 92, 731
Tru9I TTAA 3 cut(s) 29, 92, 731
TscAI CASTG 6 cut(s) 670, 702, 841, 901, 925, 1096
TseFI GTSAC 6 cut(s) 539, 784, 892, 952, 1073, 1189
TseI GCWGC 3 cut(s) 66, 285, 792
Tsp45I GTSAC 6 cut(s) 539, 784, 892, 952, 1073, 1189
TspDTI ATGAA 4 cut(s) 102, 163, 751, 1131
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 6 cut(s) 670, 702, 841, 901, 925, 1096
Tth111I GACNNNGTC 1 cut(s) 298
VpaK11BI GGWCC 2 cut(s) 130, 165
XapI RAATTY 1 cut(s) 1317
XbaI TCTAGA 1 cut(s) 497
XceI RCATGY 4 cut(s) 156, 225, 555, 960
XspI CTAG 4 cut(s) 498, 560, 828, 1187
Zsp2I ATGCAT 3 cut(s) 145, 158, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.