AT3G20300

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004011
Physical Location & Seq
Reverse (-)
7079492 .. 7082178
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20300.1

Sequence Viewer

Length: 1359 bp
ATGGCGGACATCGACATCGGAACCGCAGCAACAACAGGAGGAAACGAAGGAAGAGGAGGCACACGAGAACGTCTAATAAACCGAGAAAACAAGTTCACACGTAGCGTATCTCACGCACAAGACGAGCTCCACAGTTTCCGGAAATATCTCCGATGGATGTGCGTAGATCAGTCGAGTCCTTGGACGGCGGTTCTATCATGGTCTATGTTCGTTGTTTTCACGCTCGTTGTTCCTGCCACGTCTCACTTCATGCTCGCTTGCTCTGACTGCGATAGCCATCACTCTAGGCCGTACGATTCCGTTGTTCAGCTTTCTCTTAGCAGTTTCGCTGCTCTTTCGTTTCTTTGTCTCTCCAGATTCGTTAGCAAGTATGGTCTCCGACGGTTCCTATTCTTTGATAAGCTTTGGGATGAGAGCGAGACCGTTCGTCTAGGCTACACCAATCAGCTCAATAGATCGCTTAAGATTCTCTCCTACTTCGTGTCTCCTTGTTTCTTAGCGATGAGCTCTTATAAGATATGGTGGTATGCTTCAGGAGCTTCTCAGATTCCTTTCCTTGGTAATGTTATCTTGAGTGATACAGTTGCTTGTTTGATGGAGCTCTGCTCGTGGTTGTACCGTACTACCGTGATCTTTCTGGTTTGTGTCCTCTTCCGTCTCATCTGCCATCTCCAGATTCTTAGGCTGCAAGACTTTGCCCAGGTTTTCCAGATGGATTCAGATGTTGGTTCTATCTTGTCTGAACATCTCCGTATCAGACGTCATCTGAGAATCATCAGCCACCGATACCGAACCTTTATATTGTTGTCCTTGATATTAGTCACTGGAAGTCAGTTTTACTCTCTGCTTATTACCACTAAGGCCTACGCTGAATTGAATATCTACAGAGCTGGAGAACTAGCACTATGTTCAATGACGCTGGTCACTGCACTGCTCATTTTACTACGAAGTGCATCAAAGATCACACACAAGGCTCAGGCGGTGACTTGTCTTGCAGCCAAGTGGCATGTATGTGCGACGATAGAATCCTTTGAGACTGTGGATGGAGAGACTCCAAGGTTAGTTGATAGAGCAAGCGGACACGGATACTATCCAACAGATGATGATAATGGAGAATCAGATAGTGAAGATTATGGAGACGAAGAAGATGATTTCGATAACAACAATCTCATTCCTGCTTATGCTTATAGCACCATATCATTCCAAAAACGGCAAGCTCTAGTGAATTACTTTGAGAACAATAGATCAGGAATCACGGTGTTTGGGTTTACACTTGATAGAAGCACTCTCCATACAATCTTCGGTATCGAAATGTCGCTTGTTCTCTGGTTACTTGGTAAGACCATTGGAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

452

Amino Acids

51.33

Weight (kDa)

6.5

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 41 - 436 4.4e-175 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
AatII GACGTC 1 cut(s) 763
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 5 cut(s) 5, 24, 188, 980, 1077
AcsI RAATTY 1 cut(s) 1350
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 760
AfaI GTAC 3 cut(s) 293, 617, 622
AfiI CCNNNNNNNGG 1 cut(s) 557
AflII CTTAAG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 2 cut(s) 877, 912
AhdI GACNNNNNGTC 1 cut(s) 426
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 1 cut(s) 699
AluBI AGCT 9 cut(s) 127, 310, 403, 448, 507, 539, 601, 890, 1217
AluI AGCT 9 cut(s) 127, 310, 403, 448, 507, 539, 601, 890, 1217
Alw21I GWGCWC 3 cut(s) 129, 509, 603
Alw26I GTCTC 9 cut(s) 246, 353, 380, 413, 489, 662, 1028, 1043, 1131
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 2 cut(s) 287, 861
ApeKI GCWGC 4 cut(s) 26, 329, 685, 995
ApoI RAATTY 1 cut(s) 1350
AsuHPI GGTGA 1 cut(s) 994
BanII GRGCYC 3 cut(s) 129, 509, 603
BauI CACGAG 2 cut(s) 63, 607
Bbv12I GWGCWC 3 cut(s) 129, 509, 603
BbvI GCAGC 4 cut(s) 38, 316, 672, 1007
BccI CCATC 6 cut(s) 147, 285, 589, 675, 706, 1037
BceAI ACGGC 3 cut(s) 201, 274, 1226
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BciT130I CCWGG 1 cut(s) 701
BciVI GTATCC 1 cut(s) 1079
BcoDI GTCTC 9 cut(s) 246, 353, 380, 413, 489, 662, 1028, 1043, 1131
BfaI CTAG 4 cut(s) 285, 431, 899, 1220
BfmI CTRYAG 1 cut(s) 883
BfrI CTTAAG 1 cut(s) 461
BfuI GTATCC 1 cut(s) 1079
BisI GCNGC 4 cut(s) 27, 330, 686, 996
BlsI GCNGC 4 cut(s) 28, 331, 687, 997
Bme1390I CCNGG 1 cut(s) 701
BmeRI GACNNNNNGTC 1 cut(s) 426
BmgBI CACGTC 1 cut(s) 240
BmiI GGNNCC 2 cut(s) 22, 386
BmrFI CCNGG 1 cut(s) 701
BmsI GCATC 1 cut(s) 962
BoxI GACNNNNGTC 1 cut(s) 920
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 3 cut(s) 337, 656, 912
Bpu10I CCTNAGC 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 592
BsaAI YACGTR 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 760
BsaI GGTCTC 2 cut(s) 380, 413
BsaJI CCNNGG 4 cut(s) 179, 556, 699, 1055
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 829
Bse8I GATNNNNATC 1 cut(s) 276
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 1 cut(s) 701
BseDI CCNNGG 4 cut(s) 179, 556, 699, 1055
BseGI GGATG 3 cut(s) 162, 415, 1048
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMII CTCAG 3 cut(s) 557, 758, 989
BseNI ACTGG 1 cut(s) 829
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 4 cut(s) 38, 316, 672, 1007
BsgI GTGCAG 1 cut(s) 912
BshFI GGCC 2 cut(s) 289, 863
BsiHKAI GWGCWC 3 cut(s) 129, 509, 603
BsiSI CCGG 1 cut(s) 139
BsiWI CGTACG 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 9 cut(s) 246, 353, 380, 413, 489, 662, 1028, 1043, 1131
BsmBI CGTCTC 3 cut(s) 246, 662, 1131
BsnI GGCC 2 cut(s) 289, 863
Bso31I GGTCTC 2 cut(s) 380, 413
Bsp1286I GDGCHC 3 cut(s) 129, 509, 603
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 5 cut(s) 166, 455, 630, 960, 1244
BspACI CCGC 5 cut(s) 5, 24, 188, 980, 1077
BspANI GGCC 2 cut(s) 289, 863
BspCNI CTCAG 3 cut(s) 556, 759, 988
BspEI TCCGGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 22, 386
BspTI CTTAAG 1 cut(s) 461
BspTNI GGTCTC 2 cut(s) 380, 413
BsrI ACTGG 1 cut(s) 829
BssECI CCNNGG 4 cut(s) 179, 556, 699, 1055
BssMI GATC 5 cut(s) 166, 455, 630, 960, 1244
BssNI GRCGYC 1 cut(s) 760
BssSI CACGAG 2 cut(s) 63, 607
BssT1I CCWWGG 3 cut(s) 179, 556, 1055
Bst2BI CACGAG 2 cut(s) 63, 607
Bst2UI CCWGG 1 cut(s) 701
Bst4CI ACNGT 8 cut(s) 134, 384, 424, 583, 620, 628, 1039, 1258
Bst6I CTCTTC 2 cut(s) 46, 656
BstACI GRCGYC 1 cut(s) 760
BstAFI CTTAAG 1 cut(s) 461
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 4 cut(s) 255, 259, 1075, 1215
BstDEI CTNAG 7 cut(s) 317, 496, 543, 680, 767, 858, 975
BstF5I GGATG 3 cut(s) 162, 415, 1048
BstKTI GATC 5 cut(s) 169, 458, 633, 963, 1247
BstMAI GTCTC 9 cut(s) 246, 353, 380, 413, 489, 662, 1028, 1043, 1131
BstMBI GATC 5 cut(s) 166, 455, 630, 960, 1244
BstMWI GCNNNNNNNGC 2 cut(s) 267, 536
BstNI CCWGG 1 cut(s) 701
BstNSI RCATGY 1 cut(s) 1010
BstPAI GACNNNNGTC 1 cut(s) 920
BstSCI CCNGG 1 cut(s) 699
BstSFI CTRYAG 1 cut(s) 883
BstV1I GCAGC 4 cut(s) 38, 316, 672, 1007
BsuI GTATCC 1 cut(s) 1079
BsuRI GGCC 2 cut(s) 289, 863
BtgZI GCGATG 1 cut(s) 515
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 3 cut(s) 162, 415, 1048
BtsI GCAGTG 2 cut(s) 924, 929
BtsIMutI CAGTG 3 cut(s) 822, 924, 929
Cac8I GCNNGC 4 cut(s) 255, 259, 1075, 1215
CseI GACGC 1 cut(s) 925
Csp6I GTAC 3 cut(s) 292, 616, 621
CviAII CATG 3 cut(s) 198, 250, 1007
CviQI GTAC 3 cut(s) 292, 616, 621
DdeI CTNAG 7 cut(s) 317, 496, 543, 680, 767, 858, 975
DpnI GATC 5 cut(s) 168, 457, 632, 962, 1246
DpnII GATC 5 cut(s) 166, 455, 630, 960, 1244
DriI GACNNNNNGTC 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 46, 656
Eam1105I GACNNNNNGTC 1 cut(s) 426
EarI CTCTTC 2 cut(s) 46, 656
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 3 cut(s) 127, 507, 601
Eco130I CCWWGG 3 cut(s) 179, 556, 1055
Eco147I AGGCCT 1 cut(s) 863
Eco24I GRGCYC 3 cut(s) 129, 509, 603
Eco31I GGTCTC 2 cut(s) 380, 413
Eco53kI GAGCTC 3 cut(s) 127, 507, 601
Eco57I CTGAAG 1 cut(s) 516
EcoICRI GAGCTC 3 cut(s) 127, 507, 601
EcoRII CCWGG 1 cut(s) 699
EcoT14I CCWWGG 3 cut(s) 179, 556, 1055
EcoT38I GRGCYC 3 cut(s) 129, 509, 603
ErhI CCWWGG 3 cut(s) 179, 556, 1055
Esp3I CGTCTC 3 cut(s) 246, 662, 1131
FaeI CATG 3 cut(s) 201, 253, 1010
FatI CATG 3 cut(s) 197, 249, 1006
Fnu4HI GCNGC 4 cut(s) 27, 330, 686, 996
FokI GGATG 3 cut(s) 169, 422, 1055
FriOI GRGCYC 3 cut(s) 129, 509, 603
Fsp4HI GCNGC 4 cut(s) 27, 330, 686, 996
FspBI CTAG 4 cut(s) 285, 431, 899, 1220
GluI GCNGC 4 cut(s) 27, 330, 686, 996
GsuI CTGGAG 3 cut(s) 337, 656, 912
HaeIII GGCC 2 cut(s) 289, 863
HapII CCGG 1 cut(s) 139
HgaI GACGC 1 cut(s) 925
Hin1I GRCGYC 1 cut(s) 760
Hin1II CATG 3 cut(s) 201, 253, 1010
HindIII AAGCTT 1 cut(s) 401
HpaII CCGG 1 cut(s) 139
HphI GGTGA 1 cut(s) 994
Hpy166II GTNNAC 2 cut(s) 96, 1269
Hpy188III TCNNGA 8 cut(s) 139, 354, 534, 571, 673, 709, 1248, 1356
Hpy8I GTNNAC 2 cut(s) 96, 1269
Hpy99I CGWCG 2 cut(s) 384, 1021
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 8 cut(s) 134, 384, 424, 583, 620, 628, 1039, 1258
HpyCH4IV ACGT 4 cut(s) 70, 100, 239, 760
HpyCH4V TGCA 4 cut(s) 688, 929, 953, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 536
HpyF3I CTNAG 7 cut(s) 317, 496, 543, 680, 767, 858, 975
HpySE526I ACGT 4 cut(s) 70, 100, 239, 760
Hsp92I GRCGYC 1 cut(s) 760
Hsp92II CATG 3 cut(s) 201, 253, 1010
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 5 cut(s) 166, 455, 630, 960, 1244
LmnI GCTCC 3 cut(s) 132, 536, 598
Lsp1109I GCAGC 4 cut(s) 38, 316, 672, 1007
LweI GCATC 1 cut(s) 962
MaeI CTAG 4 cut(s) 285, 431, 899, 1220
MaeII ACGT 4 cut(s) 70, 100, 239, 760
MaeIII GTNAC 4 cut(s) 820, 922, 982, 1329
MalI GATC 5 cut(s) 168, 457, 632, 962, 1246
MboI GATC 5 cut(s) 166, 455, 630, 960, 1244
MboII GAAGA 6 cut(s) 63, 643, 1139, 1154, 1157, 1291
MhlI GDGCHC 3 cut(s) 129, 509, 603
MluCI AATT 3 cut(s) 872, 1225, 1350
MlyI GAGTC 2 cut(s) 184, 1045
MmeI TCCRAC 2 cut(s) 403, 1118
MnlI CCTC 4 cut(s) 32, 47, 50, 659
MroI TCCGGA 1 cut(s) 138
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 1 cut(s) 1011
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 1 cut(s) 701
MvaI CCWGG 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 267, 536
NdeII GATC 5 cut(s) 166, 455, 630, 960, 1244
NlaIII CATG 3 cut(s) 201, 253, 1010
NlaIV GGNNCC 2 cut(s) 22, 386
NmuCI GTSAC 3 cut(s) 820, 922, 982
NspI RCATGY 1 cut(s) 1010
PceI AGGCCT 1 cut(s) 863
PcsI WCGNNNNNNNCGW 1 cut(s) 120
Pfl23II CGTACG 1 cut(s) 291
PkrI GCNGC 4 cut(s) 28, 331, 687, 997
PleI GAGTC 2 cut(s) 183, 1045
PpsI GAGTC 2 cut(s) 183, 1045
Ppu21I YACGTR 1 cut(s) 101
PshAI GACNNNNGTC 1 cut(s) 920
PsiI TTATAA 1 cut(s) 513
Psp124BI GAGCTC 3 cut(s) 129, 509, 603
Psp6I CCWGG 1 cut(s) 699
PspGI CCWGG 1 cut(s) 699
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 22, 386
RsaI GTAC 3 cut(s) 293, 617, 622
RsaNI GTAC 3 cut(s) 292, 616, 621
RseI CAYNNNNRTG 1 cut(s) 1011
SacI GAGCTC 3 cut(s) 129, 509, 603
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 4 cut(s) 27, 330, 686, 996
Sau3AI GATC 5 cut(s) 166, 455, 630, 960, 1244
SchI GAGTC 2 cut(s) 184, 1045
ScrFI CCNGG 1 cut(s) 701
SduI GDGCHC 3 cut(s) 129, 509, 603
SfaNI GCATC 1 cut(s) 962
SfcI CTRYAG 1 cut(s) 883
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 2 cut(s) 461, 571
SmoI CTYRAG 2 cut(s) 461, 571
Sse9I AATT 3 cut(s) 872, 1225, 1350
SseBI AGGCCT 1 cut(s) 863
SsiI CCGC 5 cut(s) 5, 24, 188, 980, 1077
SspMI CTAG 4 cut(s) 285, 431, 899, 1220
SstI GAGCTC 3 cut(s) 129, 509, 603
StuI AGGCCT 1 cut(s) 863
StyD4I CCNGG 1 cut(s) 699
StyI CCWWGG 3 cut(s) 179, 556, 1055
TaaI ACNGT 8 cut(s) 134, 384, 424, 583, 620, 628, 1039, 1258
TaiI ACGT 4 cut(s) 73, 103, 242, 763
TaqI TCGA 4 cut(s) 12, 173, 1155, 1308
TasI AATT 3 cut(s) 872, 1225, 1350
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 3 cut(s) 829, 931, 936
TseFI GTSAC 3 cut(s) 820, 922, 982
TseI GCWGC 4 cut(s) 26, 329, 685, 995
Tsp45I GTSAC 3 cut(s) 820, 922, 982
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 4 cut(s) 289, 644, 740, 1098
TspRI CASTG 3 cut(s) 829, 931, 936
Vha464I CTTAAG 1 cut(s) 461
XapI RAATTY 1 cut(s) 1350
XceI RCATGY 1 cut(s) 1010
XspI CTAG 4 cut(s) 285, 431, 899, 1220
ZraI GACGTC 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.