Protein of unknown function (DUF3537)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G50630 AT1G50630 AT3G20300 |
| fragaria_vesca | FvH4_3g23460 FvH4_3g23460 FvH4_3g23460 FvH4_3g23470 |
| malus_domestica | MD11G1210400.v1.1 MD11G1210500.v1.1 |
| prunus_persica | Prupe.4G222500_v2.0.a1 Prupe.4G222600_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr5g0041291 RchiOBHm_Chr5g0041311 |
| rosa_laevigata | RLG00000034048 RLG00000034049 |
| rosa_multiflora | Rmu_co8309071.1_g000001 Rmu_sc0002715.1_g000003 Rmu_sc0015643.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00039320 Rroxscaffold_1G00039330 |
| rosa_rugosa | Rorug05G0191200 Rorug05G0191200 Rorug05G0191200 |
| rosa_samantha | Rh5AG277400 Rh5AG277500 Rh5BG282000 Rh5BG282100 Rh5CG314300 Rh5CG314400 Rh5DG290800 Rh5DG290900 |
| rosa_wichuraiana | Rw5G026080 Rw5G026110 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 239, 1281 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 686 |
| Acc36I | ACCTGC | 2 cut(s) | 239, 1281 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 144 |
| AccBSI | CCGCTC | 1 cut(s) | 285 |
| AccI | GTMKAC | 2 cut(s) | 129, 474 |
| AccII | CGCG | 2 cut(s) | 536, 1257 |
| AciI | CCGC | 9 cut(s) | 5, 15, 200, 285, 405, 543, 900, 1132, 1255 |
| AcoI | YGGCCR | 2 cut(s) | 901, 1173 |
| AfaI | GTAC | 3 cut(s) | 257, 491, 1009 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 114, 144, 521 |
| AflIII | ACRYGT | 2 cut(s) | 54, 824 |
| AgsI | TTSAA | 3 cut(s) | 94, 427, 638 |
| AjnI | CCWGG | 1 cut(s) | 572 |
| AleI | CACNNNNGTG | 2 cut(s) | 590, 1169 |
| Alw21I | GWGCWC | 1 cut(s) | 1246 |
| Alw26I | GTCTC | 4 cut(s) | 210, 377, 626, 929 |
| Ama87I | CYCGRG | 1 cut(s) | 1245 |
| AoxI | GGCC | 5 cut(s) | 251, 901, 1173, 1252, 1321 |
| ApeKI | GCWGC | 6 cut(s) | 18, 66, 271, 438, 781, 833 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 56, 98 |
| AspLEI | GCGC | 1 cut(s) | 227 |
| AspS9I | GGNCC | 3 cut(s) | 105, 1141, 1252 |
| AsuC2I | CCSGG | 1 cut(s) | 766 |
| AsuHPI | GGTGA | 1 cut(s) | 1262 |
| AsuII | TTCGAA | 1 cut(s) | 672 |
| AvaI | CYCGRG | 1 cut(s) | 1245 |
| AvaII | GGWCC | 2 cut(s) | 105, 1141 |
| BalI | TGGCCA | 1 cut(s) | 1175 |
| BanII | GRGCYC | 1 cut(s) | 771 |
| Bbv12I | GWGCWC | 1 cut(s) | 1246 |
| BbvCI | CCTCAGC | 1 cut(s) | 951 |
| BbvI | GCAGC | 6 cut(s) | 30, 53, 283, 425, 768, 820 |
| BccI | CCATC | 4 cut(s) | 1000, 1019, 1232, 1313 |
| BceAI | ACGGC | 4 cut(s) | 238, 253, 662, 897 |
| BciT130I | CCWGG | 1 cut(s) | 574 |
| BciVI | GTATCC | 1 cut(s) | 392 |
| BcnI | CCSGG | 1 cut(s) | 766 |
| BcoDI | GTCTC | 4 cut(s) | 210, 377, 626, 929 |
| BfaI | CTAG | 1 cut(s) | 1037 |
| BfuAI | ACCTGC | 2 cut(s) | 239, 1281 |
| BfuI | GTATCC | 1 cut(s) | 392 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 548 |
| BglII | AGATCT | 1 cut(s) | 419 |
| BlpI | GCTNAGC | 1 cut(s) | 281 |
| Bme1390I | CCNGG | 2 cut(s) | 574, 766 |
| Bme18I | GGWCC | 2 cut(s) | 105, 1141 |
| BmeT110I | CYCGRG | 1 cut(s) | 1245 |
| BmgT120I | GGNCC | 3 cut(s) | 105, 1141, 1252 |
| BmiI | GGNNCC | 3 cut(s) | 694, 770, 1287 |
| BmrFI | CCNGG | 2 cut(s) | 574, 766 |
| BmsI | GCATC | 2 cut(s) | 862, 1150 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 554, 586 |
| BpmI | CTGGAG | 2 cut(s) | 166, 750 |
| Bpu10I | CCTNAGC | 1 cut(s) | 951 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 281 |
| Bpu14I | TTCGAA | 1 cut(s) | 672 |
| BpuEI | CTTGAG | 1 cut(s) | 798 |
| BpuMI | CCSGG | 1 cut(s) | 766 |
| BsaWI | WCCGGW | 3 cut(s) | 42, 102, 114 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 114, 144, 521 |
| Bse118I | RCCGGY | 2 cut(s) | 1064, 1313 |
| Bse1I | ACTGG | 4 cut(s) | 133, 696, 894, 1193 |
| Bse3DI | GCAATG | 2 cut(s) | 552, 702 |
| BseBI | CCWGG | 1 cut(s) | 574 |
| BseGI | GGATG | 4 cut(s) | 126, 494, 961, 1165 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 114, 144, 521 |
| BseMI | GCAATG | 2 cut(s) | 552, 702 |
| BseMII | CTCAG | 4 cut(s) | 272, 448, 930, 942 |
| BseNI | ACTGG | 4 cut(s) | 133, 696, 894, 1193 |
| BseX3I | CGGCCG | 1 cut(s) | 901 |
| BseXI | GCAGC | 6 cut(s) | 30, 53, 283, 425, 768, 820 |
| BsgI | GTGCAG | 2 cut(s) | 290, 936 |
| Bsh1236I | CGCG | 2 cut(s) | 536, 1257 |
| Bsh1285I | CGRYCG | 3 cut(s) | 189, 591, 904 |
| BshFI | GGCC | 5 cut(s) | 253, 903, 1175, 1254, 1323 |
| BsiEI | CGRYCG | 3 cut(s) | 189, 591, 904 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1246 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1245 |
| BsiSI | CCGG | 7 cut(s) | 43, 103, 115, 197, 765, 1065, 1314 |
| BsiWI | CGTACG | 1 cut(s) | 255 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 114, 144, 521 |
| BsmAI | GTCTC | 4 cut(s) | 210, 377, 626, 929 |
| BsmBI | CGTCTC | 2 cut(s) | 377, 626 |
| BsnI | GGCC | 5 cut(s) | 253, 903, 1175, 1254, 1323 |
| BsoBI | CYCGRG | 1 cut(s) | 1245 |
| Bsp119I | TTCGAA | 1 cut(s) | 672 |
| Bsp1286I | GDGCHC | 2 cut(s) | 771, 1246 |
| Bsp143I | GATC | 2 cut(s) | 186, 419 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 281 |
| BspACI | CCGC | 9 cut(s) | 5, 15, 200, 285, 405, 543, 900, 1132, 1255 |
| BspANI | GGCC | 5 cut(s) | 253, 903, 1175, 1254, 1323 |
| BspCNI | CTCAG | 4 cut(s) | 273, 447, 931, 943 |
| BspFNI | CGCG | 2 cut(s) | 536, 1257 |
| BspLI | GGNNCC | 3 cut(s) | 694, 770, 1287 |
| BspMI | ACCTGC | 2 cut(s) | 239, 1281 |
| BspQI | GCTCTTC | 1 cut(s) | 89 |
| BspT104I | TTCGAA | 1 cut(s) | 672 |
| BsrBI | CCGCTC | 1 cut(s) | 285 |
| BsrDI | GCAATG | 2 cut(s) | 552, 702 |
| BsrFI | RCCGGY | 2 cut(s) | 1064, 1313 |
| BsrI | ACTGG | 4 cut(s) | 133, 696, 894, 1193 |
| BssAI | RCCGGY | 2 cut(s) | 1064, 1313 |
| BssMI | GATC | 2 cut(s) | 186, 419 |
| BssNAI | GTATAC | 1 cut(s) | 475 |
| Bst1107I | GTATAC | 1 cut(s) | 475 |
| Bst2UI | CCWGG | 1 cut(s) | 574 |
| Bst4CI | ACNGT | 3 cut(s) | 388, 592, 1075 |
| Bst6I | CTCTTC | 3 cut(s) | 89, 620, 1203 |
| BstBI | TTCGAA | 1 cut(s) | 672 |
| BstC8I | GCNNGC | 6 cut(s) | 78, 223, 242, 553, 1066, 1177 |
| BstDEI | CTNAG | 4 cut(s) | 281, 434, 939, 951 |
| BstF5I | GGATG | 4 cut(s) | 126, 494, 961, 1165 |
| BstFNI | CGCG | 2 cut(s) | 536, 1257 |
| BstHHI | GCGC | 1 cut(s) | 227 |
| BstKTI | GATC | 2 cut(s) | 189, 422 |
| BstMAI | GTCTC | 4 cut(s) | 210, 377, 626, 929 |
| BstMBI | GATC | 2 cut(s) | 186, 419 |
| BstMCI | CGRYCG | 3 cut(s) | 189, 591, 904 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 35, 231, 250, 277, 542, 548, 881, 890, 1314 |
| BstNI | CCWGG | 1 cut(s) | 574 |
| BstNSI | RCATGY | 4 cut(s) | 58, 555, 828, 848 |
| BstSCI | CCNGG | 2 cut(s) | 572, 764 |
| BstUI | CGCG | 2 cut(s) | 536, 1257 |
| BstV1I | GCAGC | 6 cut(s) | 30, 53, 283, 425, 768, 820 |
| BstX2I | RGATCY | 1 cut(s) | 419 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1171, 1183 |
| BstYI | RGATCY | 1 cut(s) | 419 |
| BstZ17I | GTATAC | 1 cut(s) | 475 |
| BstZI | CGGCCG | 1 cut(s) | 901 |
| BsuI | GTATCC | 1 cut(s) | 392 |
| BsuRI | GGCC | 5 cut(s) | 253, 903, 1175, 1254, 1323 |
| BtsCI | GGATG | 4 cut(s) | 126, 494, 961, 1165 |
| BtsIMutI | CAGTG | 2 cut(s) | 703, 1176 |
| BveI | ACCTGC | 2 cut(s) | 239, 1281 |
| Cac8I | GCNNGC | 6 cut(s) | 78, 223, 242, 553, 1066, 1177 |
| CfoI | GCGC | 1 cut(s) | 227 |
| Cfr10I | RCCGGY | 2 cut(s) | 1064, 1313 |
| Cfr13I | GGNCC | 3 cut(s) | 105, 1141, 1252 |
| CseI | GACGC | 2 cut(s) | 115, 253 |
| Csp6I | GTAC | 3 cut(s) | 256, 490, 1008 |
| CviAII | CATG | 7 cut(s) | 55, 448, 453, 552, 825, 845, 1111 |
| CviQI | GTAC | 3 cut(s) | 256, 490, 1008 |
| DdeI | CTNAG | 4 cut(s) | 281, 434, 939, 951 |
| DpnI | GATC | 2 cut(s) | 188, 421 |
| DpnII | GATC | 2 cut(s) | 186, 419 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 686 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 686 |
| EaeI | YGGCCR | 2 cut(s) | 901, 1173 |
| EagI | CGGCCG | 1 cut(s) | 901 |
| Eam1104I | CTCTTC | 3 cut(s) | 89, 620, 1203 |
| EarI | CTCTTC | 3 cut(s) | 89, 620, 1203 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| EclXI | CGGCCG | 1 cut(s) | 901 |
| Eco24I | GRGCYC | 1 cut(s) | 771 |
| Eco47I | GGWCC | 2 cut(s) | 105, 1141 |
| Eco52I | CGGCCG | 1 cut(s) | 901 |
| Eco88I | CYCGRG | 1 cut(s) | 1245 |
| EcoRII | CCWGG | 1 cut(s) | 572 |
| EcoT22I | ATGCAT | 2 cut(s) | 557, 1003 |
| EcoT38I | GRGCYC | 1 cut(s) | 771 |
| Esp3I | CGTCTC | 2 cut(s) | 377, 626 |
| FaeI | CATG | 7 cut(s) | 58, 451, 456, 555, 828, 848, 1114 |
| FatI | CATG | 7 cut(s) | 54, 447, 452, 551, 824, 844, 1110 |
| FauI | CCCGC | 1 cut(s) | 1125 |
| FauNDI | CATATG | 2 cut(s) | 626, 997 |
| FblI | GTMKAC | 2 cut(s) | 129, 474 |
| FokI | GGATG | 4 cut(s) | 133, 481, 968, 1172 |
| FriOI | GRGCYC | 1 cut(s) | 771 |
| FspBI | CTAG | 1 cut(s) | 1037 |
| GlaI | GCGC | 1 cut(s) | 226 |
| GsuI | CTGGAG | 2 cut(s) | 166, 750 |
| HaeIII | GGCC | 5 cut(s) | 253, 903, 1175, 1254, 1323 |
| HapII | CCGG | 7 cut(s) | 43, 103, 115, 197, 765, 1065, 1314 |
| HgaI | GACGC | 2 cut(s) | 115, 253 |
| HhaI | GCGC | 1 cut(s) | 227 |
| Hin1II | CATG | 7 cut(s) | 58, 451, 456, 555, 828, 848, 1114 |
| Hin6I | GCGC | 1 cut(s) | 225 |
| HinP1I | GCGC | 1 cut(s) | 225 |
| HincII | GTYRAC | 1 cut(s) | 130 |
| HindII | GTYRAC | 1 cut(s) | 130 |
| HindIII | AAGCTT | 3 cut(s) | 27, 365, 428 |
| HinfI | GANTC | 6 cut(s) | 511, 680, 863, 920, 1259, 1295 |
| HpaII | CCGG | 7 cut(s) | 43, 103, 115, 197, 765, 1065, 1314 |
| HphI | GGTGA | 1 cut(s) | 1262 |
| Hpy166II | GTNNAC | 6 cut(s) | 130, 475, 679, 1078, 1086, 1270 |
| Hpy188I | TCNGA | 7 cut(s) | 499, 685, 925, 1023, 1124, 1294, 1300 |
| Hpy188III | TCNNGA | 3 cut(s) | 145, 391, 1051 |
| Hpy8I | GTNNAC | 6 cut(s) | 130, 475, 679, 1078, 1086, 1270 |
| Hpy99I | CGWCG | 7 cut(s) | 89, 131, 194, 239, 269, 298, 322 |
| HpyAV | CCTTC | 1 cut(s) | 644 |
| HpyCH4III | ACNGT | 3 cut(s) | 388, 592, 1075 |
| HpyCH4IV | ACGT | 3 cut(s) | 141, 296, 1218 |
| HpyCH4V | TGCA | 7 cut(s) | 271, 555, 723, 791, 875, 917, 1001 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 35, 231, 250, 277, 542, 548, 881, 890, 1314 |
| HpyF3I | CTNAG | 4 cut(s) | 281, 434, 939, 951 |
| HpySE526I | ACGT | 3 cut(s) | 141, 296, 1218 |
| Hsp92II | CATG | 7 cut(s) | 58, 451, 456, 555, 828, 848, 1114 |
| HspAI | GCGC | 1 cut(s) | 225 |
| KroI | GCCGGC | 1 cut(s) | 1064 |
| KroNI | GCCGGC | 1 cut(s) | 1066 |
| Kzo9I | GATC | 2 cut(s) | 186, 419 |
| LguI | GCTCTTC | 1 cut(s) | 89 |
| LmnI | GCTCC | 4 cut(s) | 165, 562, 752, 774 |
| Lsp1109I | GCAGC | 6 cut(s) | 30, 53, 283, 425, 768, 820 |
| LweI | GCATC | 2 cut(s) | 862, 1150 |
| MaeI | CTAG | 1 cut(s) | 1037 |
| MaeII | ACGT | 3 cut(s) | 141, 296, 1218 |
| MaeIII | GTNAC | 4 cut(s) | 760, 820, 1039, 1214 |
| MalI | GATC | 2 cut(s) | 188, 421 |
| MbiI | CCGCTC | 1 cut(s) | 285 |
| MboI | GATC | 2 cut(s) | 186, 419 |
| MboII | GAAGA | 5 cut(s) | 106, 607, 971, 974, 1190 |
| MflI | RGATCY | 1 cut(s) | 419 |
| MhlI | GDGCHC | 2 cut(s) | 771, 1246 |
| MlsI | TGGCCA | 1 cut(s) | 1175 |
| MluCI | AATT | 2 cut(s) | 968, 1145 |
| MluNI | TGGCCA | 1 cut(s) | 1175 |
| MlyI | GAGTC | 2 cut(s) | 674, 1253 |
| MmeI | TCCRAC | 3 cut(s) | 85, 162, 1164 |
| Mox20I | TGGCCA | 1 cut(s) | 1175 |
| Mph1103I | ATGCAT | 2 cut(s) | 557, 1003 |
| MroNI | GCCGGC | 1 cut(s) | 1064 |
| MroXI | GAANNNNTTC | 2 cut(s) | 56, 98 |
| MscI | TGGCCA | 1 cut(s) | 1175 |
| MslI | CAYNNNNRTG | 3 cut(s) | 590, 849, 1169 |
| Msp20I | TGGCCA | 1 cut(s) | 1175 |
| MspA1I | CMGCKG | 3 cut(s) | 274, 438, 728 |
| MspI | CCGG | 7 cut(s) | 43, 103, 115, 197, 765, 1065, 1314 |
| MspR9I | CCNGG | 2 cut(s) | 574, 766 |
| MvaI | CCWGG | 1 cut(s) | 574 |
| MvnI | CGCG | 2 cut(s) | 536, 1257 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 35, 231, 250, 277, 542, 548, 881, 890, 1314 |
| NaeI | GCCGGC | 1 cut(s) | 1066 |
| NciI | CCSGG | 1 cut(s) | 766 |
| NdeI | CATATG | 2 cut(s) | 626, 997 |
| NdeII | GATC | 2 cut(s) | 186, 419 |
| NgoMIV | GCCGGC | 1 cut(s) | 1064 |
| NlaIII | CATG | 7 cut(s) | 58, 451, 456, 555, 828, 848, 1114 |
| NlaIV | GGNNCC | 3 cut(s) | 694, 770, 1287 |
| NmeAIII | GCCGAG | 2 cut(s) | 490, 929 |
| NmuCI | GTSAC | 2 cut(s) | 820, 1039 |
| NsiI | ATGCAT | 2 cut(s) | 557, 1003 |
| NspI | RCATGY | 4 cut(s) | 58, 555, 828, 848 |
| NspV | TTCGAA | 1 cut(s) | 672 |
| OliI | CACNNNNGTG | 2 cut(s) | 590, 1169 |
| PaeI | GCATGC | 1 cut(s) | 555 |
| PaeR7I | CTCGAG | 1 cut(s) | 1245 |
| PaqCI | CACCTGC | 2 cut(s) | 239, 1281 |
| PciI | ACATGT | 2 cut(s) | 54, 824 |
| PciSI | GCTCTTC | 1 cut(s) | 89 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 84, 264, 378 |
| PdiI | GCCGGC | 1 cut(s) | 1066 |
| PdmI | GAANNNNTTC | 2 cut(s) | 56, 98 |
| PfeI | GAWTC | 4 cut(s) | 511, 863, 920, 1295 |
| Pfl23II | CGTACG | 1 cut(s) | 255 |
| PflFI | GACNNNGTC | 2 cut(s) | 263, 298 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 144 |
| Ple19I | CGATCG | 1 cut(s) | 189 |
| PleI | GAGTC | 2 cut(s) | 674, 1253 |
| PpsI | GAGTC | 2 cut(s) | 674, 1253 |
| PscI | ACATGT | 2 cut(s) | 54, 824 |
| Psp6I | CCWGG | 1 cut(s) | 572 |
| PspGI | CCWGG | 1 cut(s) | 572 |
| PspLI | CGTACG | 1 cut(s) | 255 |
| PspN4I | GGNNCC | 3 cut(s) | 694, 770, 1287 |
| PspPI | GGNCC | 3 cut(s) | 105, 1141, 1252 |
| PspXI | VCTCGAGB | 1 cut(s) | 1245 |
| PsuI | RGATCY | 1 cut(s) | 419 |
| PsyI | GACNNNGTC | 2 cut(s) | 263, 298 |
| PvuI | CGATCG | 1 cut(s) | 189 |
| PvuII | CAGCTG | 3 cut(s) | 274, 438, 728 |
| RsaI | GTAC | 3 cut(s) | 257, 491, 1009 |
| RsaNI | GTAC | 3 cut(s) | 256, 490, 1008 |
| RseI | CAYNNNNRTG | 3 cut(s) | 590, 849, 1169 |
| SalI | GTCGAC | 1 cut(s) | 128 |
| SapI | GCTCTTC | 1 cut(s) | 89 |
| Sau3AI | GATC | 2 cut(s) | 186, 419 |
| Sau96I | GGNCC | 3 cut(s) | 105, 1141, 1252 |
| SchI | GAGTC | 2 cut(s) | 674, 1253 |
| ScrFI | CCNGG | 2 cut(s) | 574, 766 |
| SduI | GDGCHC | 2 cut(s) | 771, 1246 |
| SfaNI | GCATC | 2 cut(s) | 862, 1150 |
| Sfr274I | CTCGAG | 1 cut(s) | 1245 |
| SfuI | TTCGAA | 1 cut(s) | 672 |
| SinI | GGWCC | 2 cut(s) | 105, 1141 |
| SlaI | CTCGAG | 1 cut(s) | 1245 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 590, 849, 1169 |
| SmlI | CTYRAG | 2 cut(s) | 813, 1245 |
| SmoI | CTYRAG | 2 cut(s) | 813, 1245 |
| SphI | GCATGC | 1 cut(s) | 555 |
| Sse9I | AATT | 2 cut(s) | 968, 1145 |
| SsiI | CCGC | 9 cut(s) | 5, 15, 200, 285, 405, 543, 900, 1132, 1255 |
| SspI | AATATT | 1 cut(s) | 529 |
| SspMI | CTAG | 1 cut(s) | 1037 |
| StyD4I | CCNGG | 2 cut(s) | 572, 764 |
| TaaI | ACNGT | 3 cut(s) | 388, 592, 1075 |
| TaiI | ACGT | 3 cut(s) | 144, 299, 1221 |
| TaqI | TCGA | 5 cut(s) | 129, 672, 1050, 1137, 1246 |
| TasI | AATT | 2 cut(s) | 968, 1145 |
| TauI | GCSGC | 5 cut(s) | 18, 288, 545, 903, 1257 |
| TfiI | GAWTC | 4 cut(s) | 511, 863, 920, 1295 |
| TscAI | CASTG | 2 cut(s) | 703, 1176 |
| TseFI | GTSAC | 2 cut(s) | 820, 1039 |
| TseI | GCWGC | 6 cut(s) | 18, 66, 271, 438, 781, 833 |
| Tsp45I | GTSAC | 2 cut(s) | 820, 1039 |
| TspDTI | ATGAA | 2 cut(s) | 972, 981 |
| TspGWI | ACGGA | 2 cut(s) | 608, 902 |
| TspRI | CASTG | 2 cut(s) | 703, 1176 |
| Tth111I | GACNNNGTC | 2 cut(s) | 263, 298 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 144 |
| VpaK11BI | GGWCC | 2 cut(s) | 105, 1141 |
| XceI | RCATGY | 4 cut(s) | 58, 555, 828, 848 |
| XhoI | CTCGAG | 1 cut(s) | 1245 |
| XmiI | GTMKAC | 2 cut(s) | 129, 474 |
| XmnI | GAANNNNTTC | 2 cut(s) | 56, 98 |
| XspI | CTAG | 1 cut(s) | 1037 |
| Zsp2I | ATGCAT | 2 cut(s) | 557, 1003 |