RchiOBHm_Chr5g0041291

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004011
Physical Location & Seq
Forward (+)
36048838 .. 36052284
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31973

Sequence Viewer

Length: 1311 bp
ATGGCGGACGAAAGCGGCAGCAGAGAAGCTTTGTTAGCCAACCGGAGAAAGAACATGTTCCAACGCTGCGTTTCGCACGCCCACGACGAGTTGAAGAGCTTCCGGTCCTATCTCCGGTGGATGTGCGTTGACCAGTCCAACGTCTGGAGATTGTGCCTCTCTTGGAGCGTTTTCGTTGTTTTTACTATCGTCGTTCCGGCGGTGTCTCATTTTCTTTTCGCCTGCTCCACCTGCGACGGCAAGCACAAGAGGCCGTACGACAGCGTCGTGCAGCTGTCGCTGAGCGGCGTGGCGACGTTGTCGTTTGTGTGCCTGTCGTCTTTTGTGAGGAAGTATGGGCTGAAAAGGTTTCTGTTTTTTGATAAGCTTTACGATGAGAGCGAGACGGTCAGGAGGGGATACACCGCACAGCTTAATAGATCTTTGAAGCTTCTCAGCTGCTTTGTCATACCATGCTTTGTTGCCGAGAGTGTATACAAGATATGGTGGTACTCATCCGAAGCCTCGCAGATTCCTTATGTGGGCAATATTTACGCGAGTGCCGCAATGGCATGCATAATGGAGCTATTCTCCTGGCTCTATCGCACGACCGTGATTTTCCTCGTGTGTGTTCTCTTCCGTCTCATATGCTACCTTCAAATCCTACGGCTACAAGATTTTGCCACAATGTTCGAAGTGGACTCTGATGTCGGATCAGTGTTATCGGAGCACCTTAGGATCCGAAGGCACCTGAGGATCATAAGCCACAGGTGCCGCGATTTCGTATTGTGGAAACTCATTTTGGTCACGGGGAGCCAATTTGCTCTGTTGCTCATCACAACCAGTTCCAGTGCTATTGCCAACCTTGATATTTGCACAGCTGGAGAACTTCTGTTTTGCTCCATAACTCTAGTTACCGGGCTCCTCATCGTGCTGCGCAGTGCAACCAAAATCACACACAAGGCGCAAGCAGTCACATGTCTTGCTGCCAAATGGCATGTCTGTGCCACATTAGATTCGTTTGATGCAACAACGGCTCAGATCGCCAGTAACGGCCGAGTGTTCCTTGCAGAATCCGATAACGATGAGACTGAGGACGAGGCTGAGGATGAAGAAGATGAATTGGACAACAGCAAGTTGATACCATCATATGCATATAGTACCATCTCTTATCAGAAAAGACAAGCTCTAGTGACATATTTCGAGAACAACAGAGCCGGCATTACTGTTTACGGGTTTACATTGGACAGGACTACGCTACACATGTTATTTATGATTGAATTTTCATTAATTCTCTGGTTGTTGGGGAAGACCATTAATGGAATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

436

Amino Acids

49.65

Weight (kDa)

7.49

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 29 - 419 1.5e-169 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 686
Acc16I TGCGCA 1 cut(s) 917
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 2 cut(s) 726, 750
AccB7I CCANNNNNTGG 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 285
AccI GTMKAC 1 cut(s) 474
AccII CGCG 2 cut(s) 536, 756
AciI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
AclWI GGATC 4 cut(s) 700, 712, 725, 743
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 2 cut(s) 1259, 1302
AfaI GTAC 3 cut(s) 257, 491, 1141
AfiI CCNNNNNNNGG 3 cut(s) 114, 144, 521
AflIII ACRYGT 3 cut(s) 54, 956, 1242
AgsI TTSAA 4 cut(s) 94, 427, 638, 1259
AjnI CCWGG 1 cut(s) 572
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AleI CACNNNNGTG 1 cut(s) 590
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 4 cut(s) 210, 377, 626, 1061
AlwI GGATC 4 cut(s) 700, 712, 725, 743
AoxI GGCC 2 cut(s) 251, 1033
ApeKI GCWGC 6 cut(s) 18, 66, 271, 438, 913, 965
ApoI RAATTY 2 cut(s) 1259, 1302
ArsI GACNNNNNNTTYG 2 cut(s) 963, 995
AseI ATTAAT 2 cut(s) 1268, 1296
Asp700I GAANNNNTTC 2 cut(s) 56, 98
AspLEI GCGC 2 cut(s) 918, 946
AspS9I GGNCC 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 898
AsuII TTCGAA 1 cut(s) 672
AvaII GGWCC 1 cut(s) 105
AxyI CCTNAGG 2 cut(s) 713, 731
BamHI GGATCC 1 cut(s) 717
BanI GGYRCC 2 cut(s) 726, 750
BanII GRGCYC 1 cut(s) 903
BauI CACGAG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 1295
Bbv12I GWGCWC 1 cut(s) 711
BbvCI CCTCAGC 1 cut(s) 1083
BbvI GCAGC 6 cut(s) 30, 53, 283, 425, 900, 952
BccI CCATC 2 cut(s) 1132, 1151
BceAI ACGGC 5 cut(s) 238, 253, 662, 1029, 1048
BciT130I CCWGG 1 cut(s) 574
BciVI GTATCC 1 cut(s) 392
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 4 cut(s) 210, 377, 626, 1061
BfaI CTAG 2 cut(s) 890, 1169
BfuAI ACCTGC 1 cut(s) 239
BfuI GTATCC 1 cut(s) 392
BglI GCCNNNNNGGC 1 cut(s) 548
BglII AGATCT 1 cut(s) 419
BlpI GCTNAGC 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 574, 898
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 5 cut(s) 719, 728, 752, 794, 902
BmrFI CCNGG 2 cut(s) 574, 898
BmsI GCATC 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 1295
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 2 cut(s) 166, 882
Bpu10I CCTNAGC 1 cut(s) 1083
Bpu1102I GCTNAGC 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 898
BsaWI WCCGGW 3 cut(s) 42, 102, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 144, 521
Bse118I RCCGGY 1 cut(s) 1196
Bse1I ACTGG 4 cut(s) 133, 822, 828, 1026
Bse21I CCTNAGG 2 cut(s) 713, 731
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 574
BseGI GGATG 3 cut(s) 126, 494, 1093
BseLI CCNNNNNNNGG 3 cut(s) 114, 144, 521
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 6 cut(s) 272, 448, 722, 1031, 1062, 1074
BseNI ACTGG 4 cut(s) 133, 822, 828, 1026
BseRI GAGGAG 1 cut(s) 893
BseX3I CGGCCG 1 cut(s) 1033
BseXI GCAGC 6 cut(s) 30, 53, 283, 425, 900, 952
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 2 cut(s) 536, 756
Bsh1285I CGRYCG 2 cut(s) 591, 1036
BshFI GGCC 2 cut(s) 253, 1035
BshNI GGYRCC 2 cut(s) 726, 750
BsiEI CGRYCG 2 cut(s) 591, 1036
BsiHKAI GWGCWC 1 cut(s) 711
BsiSI CCGG 6 cut(s) 43, 103, 115, 197, 897, 1197
BsiWI CGTACG 1 cut(s) 255
BslI CCNNNNNNNGG 3 cut(s) 114, 144, 521
BsmAI GTCTC 4 cut(s) 210, 377, 626, 1061
BsmBI CGTCTC 2 cut(s) 377, 626
BsnI GGCC 2 cut(s) 253, 1035
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 2 cut(s) 711, 903
Bsp143I GATC 5 cut(s) 419, 692, 717, 735, 1020
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
BspANI GGCC 2 cut(s) 253, 1035
BspCNI CTCAG 6 cut(s) 273, 447, 723, 1030, 1063, 1075
BspFNI CGCG 2 cut(s) 536, 756
BspLI GGNNCC 5 cut(s) 719, 728, 752, 794, 902
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 4 cut(s) 700, 712, 725, 743
BspQI GCTCTTC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 672
BspT107I GGYRCC 2 cut(s) 726, 750
BsrBI CCGCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 1196
BsrI ACTGG 4 cut(s) 133, 822, 828, 1026
BssAI RCCGGY 1 cut(s) 1196
BssMI GATC 5 cut(s) 419, 692, 717, 735, 1020
BssNAI GTATAC 1 cut(s) 475
BssSI CACGAG 1 cut(s) 602
Bst1107I GTATAC 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 3 cut(s) 388, 592, 1207
Bst6I CTCTTC 2 cut(s) 89, 620
BstBI TTCGAA 1 cut(s) 672
BstC8I GCNNGC 6 cut(s) 78, 223, 242, 553, 948, 1198
BstDEI CTNAG 7 cut(s) 281, 434, 713, 731, 1017, 1071, 1083
BstF5I GGATG 3 cut(s) 126, 494, 1093
BstFNI CGCG 2 cut(s) 536, 756
BstHHI GCGC 2 cut(s) 918, 946
BstKTI GATC 5 cut(s) 422, 695, 720, 738, 1023
BstMAI GTCTC 4 cut(s) 210, 377, 626, 1061
BstMBI GATC 5 cut(s) 419, 692, 717, 735, 1020
BstMCI CGRYCG 2 cut(s) 591, 1036
BstMWI GCNNNNNNNGC 9 cut(s) 35, 231, 250, 277, 542, 548, 750, 1013, 1022
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 5 cut(s) 58, 555, 960, 980, 1246
BstSCI CCNGG 2 cut(s) 572, 896
BstUI CGCG 2 cut(s) 536, 756
BstV1I GCAGC 6 cut(s) 30, 53, 283, 425, 900, 952
BstV2I GAAGAC 1 cut(s) 1295
BstX2I RGATCY 2 cut(s) 419, 717
BstYI RGATCY 2 cut(s) 419, 717
BstZ17I GTATAC 1 cut(s) 475
BstZI CGGCCG 1 cut(s) 1033
Bsu36I CCTNAGG 2 cut(s) 713, 731
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 253, 1035
BtsCI GGATG 3 cut(s) 126, 494, 1093
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 3 cut(s) 702, 835, 925
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 6 cut(s) 78, 223, 242, 553, 948, 1198
CfoI GCGC 2 cut(s) 918, 946
Cfr10I RCCGGY 1 cut(s) 1196
Cfr13I GGNCC 1 cut(s) 105
CseI GACGC 1 cut(s) 253
Csp6I GTAC 3 cut(s) 256, 490, 1140
CviAII CATG 6 cut(s) 55, 453, 552, 957, 977, 1243
CviQI GTAC 3 cut(s) 256, 490, 1140
DdeI CTNAG 7 cut(s) 281, 434, 713, 731, 1017, 1071, 1083
DpnI GATC 5 cut(s) 421, 694, 719, 737, 1022
DpnII GATC 5 cut(s) 419, 692, 717, 735, 1020
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 1033
EagI CGGCCG 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 89, 620
EarI CTCTTC 2 cut(s) 89, 620
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 1033
Eco24I GRGCYC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 105
Eco52I CGGCCG 1 cut(s) 1033
Eco81I CCTNAGG 2 cut(s) 713, 731
EcoRII CCWGG 1 cut(s) 572
EcoT22I ATGCAT 2 cut(s) 557, 1135
EcoT38I GRGCYC 1 cut(s) 903
Esp3I CGTCTC 2 cut(s) 377, 626
FaeI CATG 6 cut(s) 58, 456, 555, 960, 980, 1246
FatI CATG 6 cut(s) 54, 452, 551, 956, 976, 1242
FauNDI CATATG 2 cut(s) 626, 1129
FblI GTMKAC 1 cut(s) 474
FokI GGATG 3 cut(s) 133, 481, 1100
FriOI GRGCYC 1 cut(s) 903
FspBI CTAG 2 cut(s) 890, 1169
FspI TGCGCA 1 cut(s) 917
GlaI GCGC 2 cut(s) 917, 945
GsuI CTGGAG 2 cut(s) 166, 882
HaeIII GGCC 2 cut(s) 253, 1035
HapII CCGG 6 cut(s) 43, 103, 115, 197, 897, 1197
HgaI GACGC 1 cut(s) 253
HhaI GCGC 2 cut(s) 918, 946
Hin1II CATG 6 cut(s) 58, 456, 555, 960, 980, 1246
Hin6I GCGC 2 cut(s) 916, 944
HinP1I GCGC 2 cut(s) 916, 944
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 3 cut(s) 27, 365, 428
HinfI GANTC 4 cut(s) 511, 680, 995, 1052
HpaII CCGG 6 cut(s) 43, 103, 115, 197, 897, 1197
Hpy166II GTNNAC 5 cut(s) 130, 475, 679, 1210, 1218
Hpy188I TCNGA 8 cut(s) 499, 685, 692, 706, 722, 1020, 1057, 1155
Hpy188III TCNNGA 3 cut(s) 145, 391, 1183
Hpy8I GTNNAC 5 cut(s) 130, 475, 679, 1210, 1218
Hpy99I CGWCG 5 cut(s) 89, 194, 239, 269, 298
HpyAV CCTTC 2 cut(s) 644, 717
HpyCH4III ACNGT 3 cut(s) 388, 592, 1207
HpyCH4IV ACGT 2 cut(s) 141, 296
HpyCH4V TGCA 7 cut(s) 271, 555, 855, 923, 1007, 1049, 1133
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 231, 250, 277, 542, 548, 750, 1013, 1022
HpyF3I CTNAG 7 cut(s) 281, 434, 713, 731, 1017, 1071, 1083
HpySE526I ACGT 2 cut(s) 141, 296
Hsp92II CATG 6 cut(s) 58, 456, 555, 960, 980, 1246
HspAI GCGC 2 cut(s) 916, 944
KroI GCCGGC 1 cut(s) 1196
KroNI GCCGGC 1 cut(s) 1198
Kzo9I GATC 5 cut(s) 419, 692, 717, 735, 1020
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 7 cut(s) 165, 230, 562, 706, 792, 884, 906
Lsp1109I GCAGC 6 cut(s) 30, 53, 283, 425, 900, 952
LweI GCATC 1 cut(s) 994
MaeI CTAG 2 cut(s) 890, 1169
MaeII ACGT 2 cut(s) 141, 296
MaeIII GTNAC 5 cut(s) 784, 892, 952, 1028, 1171
MalI GATC 5 cut(s) 421, 694, 719, 737, 1022
MbiI CCGCTC 1 cut(s) 285
MboI GATC 5 cut(s) 419, 692, 717, 735, 1020
MboII GAAGA 5 cut(s) 106, 607, 1103, 1106, 1300
MflI RGATCY 2 cut(s) 419, 717
MhlI GDGCHC 2 cut(s) 711, 903
MluCI AATT 5 cut(s) 797, 1100, 1259, 1269, 1302
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 3 cut(s) 85, 162, 670
Mph1103I ATGCAT 2 cut(s) 557, 1135
MroNI GCCGGC 1 cut(s) 1196
MroXI GAANNNNTTC 2 cut(s) 56, 98
MseI TTAA 4 cut(s) 414, 1268, 1296, 1309
MslI CAYNNNNRTG 2 cut(s) 590, 981
MspA1I CMGCKG 3 cut(s) 274, 438, 860
MspI CCGG 6 cut(s) 43, 103, 115, 197, 897, 1197
MspR9I CCNGG 2 cut(s) 574, 898
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 2 cut(s) 536, 756
MwoI GCNNNNNNNGC 9 cut(s) 35, 231, 250, 277, 542, 548, 750, 1013, 1022
NaeI GCCGGC 1 cut(s) 1198
NciI CCSGG 1 cut(s) 898
NdeI CATATG 2 cut(s) 626, 1129
NdeII GATC 5 cut(s) 419, 692, 717, 735, 1020
NgoMIV GCCGGC 1 cut(s) 1196
NlaIII CATG 6 cut(s) 58, 456, 555, 960, 980, 1246
NlaIV GGNNCC 5 cut(s) 719, 728, 752, 794, 902
NmeAIII GCCGAG 2 cut(s) 490, 1061
NmuCI GTSAC 3 cut(s) 784, 952, 1171
NsbI TGCGCA 1 cut(s) 917
NsiI ATGCAT 2 cut(s) 557, 1135
NspI RCATGY 5 cut(s) 58, 555, 960, 980, 1246
NspV TTCGAA 1 cut(s) 672
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 239
PciI ACATGT 3 cut(s) 54, 956, 1242
PciSI GCTCTTC 1 cut(s) 89
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 264, 378
PdiI GCCGGC 1 cut(s) 1198
PdmI GAANNNNTTC 2 cut(s) 56, 98
PfeI GAWTC 3 cut(s) 511, 995, 1052
Pfl23II CGTACG 1 cut(s) 255
PflFI GACNNNGTC 2 cut(s) 263, 298
PflMI CCANNNNNTGG 1 cut(s) 144
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
PscI ACATGT 3 cut(s) 54, 956, 1242
PshBI ATTAAT 2 cut(s) 1268, 1296
Psp6I CCWGG 1 cut(s) 572
PspGI CCWGG 1 cut(s) 572
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 5 cut(s) 719, 728, 752, 794, 902
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 2 cut(s) 419, 717
PsyI GACNNNGTC 2 cut(s) 263, 298
PvuII CAGCTG 3 cut(s) 274, 438, 860
RsaI GTAC 3 cut(s) 257, 491, 1141
RsaNI GTAC 3 cut(s) 256, 490, 1140
RseI CAYNNNNRTG 2 cut(s) 590, 981
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 4 cut(s) 414, 1268, 1296, 1309
Sau3AI GATC 5 cut(s) 419, 692, 717, 735, 1020
Sau96I GGNCC 1 cut(s) 105
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 2 cut(s) 574, 898
SduI GDGCHC 2 cut(s) 711, 903
SfaNI GCATC 1 cut(s) 994
SfuI TTCGAA 1 cut(s) 672
SinI GGWCC 1 cut(s) 105
SmiMI CAYNNNNRTG 2 cut(s) 590, 981
SphI GCATGC 1 cut(s) 555
Sse9I AATT 5 cut(s) 797, 1100, 1259, 1269, 1302
SsiI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 890, 1169
StyD4I CCNGG 2 cut(s) 572, 896
TaaI ACNGT 3 cut(s) 388, 592, 1207
TaiI ACGT 2 cut(s) 144, 299
TaqI TCGA 2 cut(s) 672, 1182
TasI AATT 5 cut(s) 797, 1100, 1259, 1269, 1302
TauI GCSGC 4 cut(s) 18, 288, 545, 756
TfiI GAWTC 3 cut(s) 511, 995, 1052
Tru1I TTAA 4 cut(s) 414, 1268, 1296, 1309
Tru9I TTAA 4 cut(s) 414, 1268, 1296, 1309
TscAI CASTG 3 cut(s) 702, 835, 925
TseFI GTSAC 3 cut(s) 784, 952, 1171
TseI GCWGC 6 cut(s) 18, 66, 271, 438, 913, 965
Tsp45I GTSAC 3 cut(s) 784, 952, 1171
TspDTI ATGAA 3 cut(s) 1104, 1113, 1254
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 3 cut(s) 702, 835, 925
Tth111I GACNNNGTC 2 cut(s) 263, 298
Van91I CCANNNNNTGG 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 105
VspI ATTAAT 2 cut(s) 1268, 1296
XapI RAATTY 2 cut(s) 1259, 1302
XceI RCATGY 5 cut(s) 58, 555, 960, 980, 1246
XmiI GTMKAC 1 cut(s) 474
XmnI GAANNNNTTC 2 cut(s) 56, 98
XspI CTAG 2 cut(s) 890, 1169
Zsp2I ATGCAT 2 cut(s) 557, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.