Rh5DG290800

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004011
Physical Location & Seq
Forward (+)
36327584 .. 36329844
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG290800.1

Sequence Viewer

Length: 897 bp
ATGGCGGACGAAAGCGGCAGCAGAGAAGCTTTGTTAGCCAACCGGAGAAAGAACATGTTCCAACGCTGCGTTTCGCACGCCCACGACGAGTTGAAGAGCTTCCGGTCCTATCTCCGGTGGATGTGCGTCGACCAGTCCAACGTCTGGAGATTGTGCCTCTCTTGGAGCGTTTTCGTTGTTTTTACGATCGTCGTTCCGGCGGTGTCTCATTTTGTATACGCCTGCTCCACCTGCGACGGCAAGCACAAGAGGCCGTACGACAGCGTCGTGCAGCTGTCGCTGAGCGGCGTGGCGACGCTGTCGTTTGTGTGCCTGTCGTCGTTTGTGAGGAAGTATGGGCTGAAAAGGTTTCTGTTTTTTGATAAGCTTTACGATGAGAGCGAGACGGTCAGGAGGGGATACACCGCGCAGCTCAATAGATCTTTGAAGCTTCTCAGCTGCTTTGTCATACCATGCTTTGTTGCCGAGAGTGTATACAAGATATGGTGGTACTCATCCGAAGCCTCGCAGATTCCTTATGTGGGCAATATTTACGCGAGTGCCGCAATGGCATGCATAATGGAGCTATTCTCCTGGCTCTATCGCACGACCGTGATTTTCCTCGTGTGTGTTCTCTTCCGTCTCATATGCTACCTTCAAATCCTACGGCTACAAGATTTTGCCTCAATGTTCGAAGTGGACTCTGATGTCGGATCAGTGTTATCGGAGCACCTTAGGATCCGAAGGCACCTGAGGATCATAAGCCACAGGTGCCGCGATTTCGTATTGTGGAAACTCATTTTGGTCACGGGGAGCCAATTTGCTCTGTTGCTCATCACAACCAGTTCCAGTGCTATTGCCAACCTTGATATTTGCACAGCTGGAGAACTTCTGGTATGCTTCCTTTTCCCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

298

Amino Acids

34.28

Weight (kDa)

8.92

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 29 - 293 5.6e-112 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 686
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 2 cut(s) 726, 750
AccB7I CCANNNNNTGG 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 285
AccI GTMKAC 3 cut(s) 129, 216, 474
AccII CGCG 3 cut(s) 407, 536, 756
AciI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
AclWI GGATC 4 cut(s) 700, 712, 725, 743
AfaI GTAC 2 cut(s) 257, 491
AfiI CCNNNNNNNGG 3 cut(s) 114, 144, 521
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 3 cut(s) 94, 427, 638
AjnI CCWGG 1 cut(s) 572
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AleI CACNNNNGTG 1 cut(s) 590
AluBI AGCT 9 cut(s) 29, 99, 274, 367, 412, 430, 438, 565, 860
AluI AGCT 9 cut(s) 29, 99, 274, 367, 412, 430, 438, 565, 860
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 3 cut(s) 210, 377, 626
AlwI GGATC 4 cut(s) 700, 712, 725, 743
AoxI GGCC 1 cut(s) 251
ApeKI GCWGC 5 cut(s) 18, 66, 271, 409, 438
Asp700I GAANNNNTTC 2 cut(s) 56, 98
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 105
AsuII TTCGAA 1 cut(s) 672
AvaII GGWCC 1 cut(s) 105
AxyI CCTNAGG 2 cut(s) 713, 731
BamHI GGATCC 1 cut(s) 717
BanI GGYRCC 2 cut(s) 726, 750
BauI CACGAG 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 711
BbvI GCAGC 5 cut(s) 30, 53, 283, 421, 425
BceAI ACGGC 3 cut(s) 238, 253, 662
BciT130I CCWGG 1 cut(s) 574
BciVI GTATCC 1 cut(s) 392
BcoDI GTCTC 3 cut(s) 210, 377, 626
BfuAI ACCTGC 1 cut(s) 239
BfuI GTATCC 1 cut(s) 392
BglI GCCNNNNNGGC 1 cut(s) 548
BglII AGATCT 1 cut(s) 419
BisI GCNGC 9 cut(s) 16, 19, 67, 272, 286, 410, 439, 543, 754
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 9 cut(s) 17, 20, 68, 273, 287, 411, 440, 544, 755
Bme1390I CCNGG 1 cut(s) 574
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 4 cut(s) 719, 728, 752, 794
BmrFI CCNGG 1 cut(s) 574
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 2 cut(s) 166, 882
Bpu1102I GCTNAGC 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 672
BsaWI WCCGGW 3 cut(s) 42, 102, 114
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 144, 521
Bse1I ACTGG 3 cut(s) 133, 822, 828
Bse21I CCTNAGG 2 cut(s) 713, 731
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 574
BseGI GGATG 2 cut(s) 126, 494
BseLI CCNNNNNNNGG 3 cut(s) 114, 144, 521
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 272, 448, 722
BseNI ACTGG 3 cut(s) 133, 822, 828
BseXI GCAGC 5 cut(s) 30, 53, 283, 421, 425
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 3 cut(s) 407, 536, 756
Bsh1285I CGRYCG 2 cut(s) 189, 591
BshFI GGCC 1 cut(s) 253
BshNI GGYRCC 2 cut(s) 726, 750
BsiEI CGRYCG 2 cut(s) 189, 591
BsiHKAI GWGCWC 1 cut(s) 711
BsiSI CCGG 4 cut(s) 43, 103, 115, 197
BsiWI CGTACG 1 cut(s) 255
BslI CCNNNNNNNGG 3 cut(s) 114, 144, 521
BsmAI GTCTC 3 cut(s) 210, 377, 626
BsmBI CGTCTC 2 cut(s) 377, 626
BsnI GGCC 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 1 cut(s) 711
Bsp143I GATC 5 cut(s) 186, 419, 692, 717, 735
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
BspANI GGCC 1 cut(s) 253
BspCNI CTCAG 3 cut(s) 273, 447, 723
BspFNI CGCG 3 cut(s) 407, 536, 756
BspHI TCATGA 1 cut(s) 893
BspLI GGNNCC 4 cut(s) 719, 728, 752, 794
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 4 cut(s) 700, 712, 725, 743
BspQI GCTCTTC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 672
BspT107I GGYRCC 2 cut(s) 726, 750
BsrBI CCGCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 3 cut(s) 133, 822, 828
BssMI GATC 5 cut(s) 186, 419, 692, 717, 735
BssNAI GTATAC 2 cut(s) 217, 475
BssSI CACGAG 1 cut(s) 602
Bst1107I GTATAC 2 cut(s) 217, 475
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 2 cut(s) 388, 592
Bst6I CTCTTC 2 cut(s) 89, 620
BstBI TTCGAA 1 cut(s) 672
BstC8I GCNNGC 4 cut(s) 78, 223, 242, 553
BstDEI CTNAG 4 cut(s) 281, 434, 713, 731
BstF5I GGATG 2 cut(s) 126, 494
BstFNI CGCG 3 cut(s) 407, 536, 756
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 5 cut(s) 189, 422, 695, 720, 738
BstMAI GTCTC 3 cut(s) 210, 377, 626
BstMBI GATC 5 cut(s) 186, 419, 692, 717, 735
BstMCI CGRYCG 2 cut(s) 189, 591
BstMWI GCNNNNNNNGC 7 cut(s) 35, 231, 250, 277, 542, 548, 750
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 2 cut(s) 58, 555
BstSCI CCNGG 1 cut(s) 572
BstUI CGCG 3 cut(s) 407, 536, 756
BstV1I GCAGC 5 cut(s) 30, 53, 283, 421, 425
BstX2I RGATCY 2 cut(s) 419, 717
BstYI RGATCY 2 cut(s) 419, 717
BstZ17I GTATAC 2 cut(s) 217, 475
Bsu36I CCTNAGG 2 cut(s) 713, 731
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 1 cut(s) 253
BtsCI GGATG 2 cut(s) 126, 494
BtsIMutI CAGTG 2 cut(s) 702, 835
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 4 cut(s) 78, 223, 242, 553
CciI TCATGA 1 cut(s) 893
CfoI GCGC 1 cut(s) 409
Cfr13I GGNCC 1 cut(s) 105
CseI GACGC 3 cut(s) 115, 253, 304
Csp6I GTAC 2 cut(s) 256, 490
CviAII CATG 4 cut(s) 55, 453, 552, 894
CviQI GTAC 2 cut(s) 256, 490
DdeI CTNAG 4 cut(s) 281, 434, 713, 731
DpnI GATC 5 cut(s) 188, 421, 694, 719, 737
DpnII GATC 5 cut(s) 186, 419, 692, 717, 735
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
Eam1104I CTCTTC 2 cut(s) 89, 620
EarI CTCTTC 2 cut(s) 89, 620
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 105
Eco81I CCTNAGG 2 cut(s) 713, 731
EcoRII CCWGG 1 cut(s) 572
EcoT22I ATGCAT 1 cut(s) 557
Esp3I CGTCTC 2 cut(s) 377, 626
FaeI CATG 4 cut(s) 58, 456, 555, 897
FatI CATG 4 cut(s) 54, 452, 551, 893
FauNDI CATATG 1 cut(s) 626
FblI GTMKAC 3 cut(s) 129, 216, 474
Fnu4HI GCNGC 9 cut(s) 16, 19, 67, 272, 286, 410, 439, 543, 754
FokI GGATG 2 cut(s) 133, 481
Fsp4HI GCNGC 9 cut(s) 16, 19, 67, 272, 286, 410, 439, 543, 754
GlaI GCGC 1 cut(s) 408
GluI GCNGC 9 cut(s) 16, 19, 67, 272, 286, 410, 439, 543, 754
GsuI CTGGAG 2 cut(s) 166, 882
HaeIII GGCC 1 cut(s) 253
HapII CCGG 4 cut(s) 43, 103, 115, 197
HgaI GACGC 3 cut(s) 115, 253, 304
HhaI GCGC 1 cut(s) 409
Hin1II CATG 4 cut(s) 58, 456, 555, 897
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 3 cut(s) 27, 365, 428
HinfI GANTC 2 cut(s) 511, 680
HpaII CCGG 4 cut(s) 43, 103, 115, 197
Hpy166II GTNNAC 4 cut(s) 130, 217, 475, 679
Hpy188I TCNGA 5 cut(s) 499, 685, 692, 706, 722
Hpy188III TCNNGA 3 cut(s) 145, 391, 894
Hpy8I GTNNAC 4 cut(s) 130, 217, 475, 679
Hpy99I CGWCG 7 cut(s) 89, 131, 194, 239, 269, 298, 322
HpyAV CCTTC 2 cut(s) 644, 717
HpyCH4III ACNGT 2 cut(s) 388, 592
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 271, 555, 855
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 231, 250, 277, 542, 548, 750
HpyF3I CTNAG 4 cut(s) 281, 434, 713, 731
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 4 cut(s) 58, 456, 555, 897
HspAI GCGC 1 cut(s) 407
Kzo9I GATC 5 cut(s) 186, 419, 692, 717, 735
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 5 cut(s) 165, 230, 562, 706, 792
Lsp1109I GCAGC 5 cut(s) 30, 53, 283, 421, 425
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 1 cut(s) 784
MalI GATC 5 cut(s) 188, 421, 694, 719, 737
MbiI CCGCTC 1 cut(s) 285
MboI GATC 5 cut(s) 186, 419, 692, 717, 735
MboII GAAGA 2 cut(s) 106, 607
MflI RGATCY 2 cut(s) 419, 717
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 1 cut(s) 797
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 3 cut(s) 85, 162, 670
MnlI CCTC 8 cut(s) 167, 243, 321, 387, 514, 611, 673, 726
Mph1103I ATGCAT 1 cut(s) 557
MroXI GAANNNNTTC 2 cut(s) 56, 98
MslI CAYNNNNRTG 1 cut(s) 590
MspA1I CMGCKG 3 cut(s) 274, 438, 860
MspI CCGG 4 cut(s) 43, 103, 115, 197
MspR9I CCNGG 1 cut(s) 574
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 3 cut(s) 407, 536, 756
MwoI GCNNNNNNNGC 7 cut(s) 35, 231, 250, 277, 542, 548, 750
NdeI CATATG 1 cut(s) 626
NdeII GATC 5 cut(s) 186, 419, 692, 717, 735
NlaIII CATG 4 cut(s) 58, 456, 555, 897
NlaIV GGNNCC 4 cut(s) 719, 728, 752, 794
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 2 cut(s) 58, 555
NspV TTCGAA 1 cut(s) 672
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 555
PagI TCATGA 1 cut(s) 893
PaqCI CACCTGC 1 cut(s) 239
PciI ACATGT 1 cut(s) 54
PciSI GCTCTTC 1 cut(s) 89
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 264, 378
PdmI GAANNNNTTC 2 cut(s) 56, 98
PfeI GAWTC 1 cut(s) 511
Pfl23II CGTACG 1 cut(s) 255
PflFI GACNNNGTC 2 cut(s) 263, 298
PflMI CCANNNNNTGG 1 cut(s) 144
PkrI GCNGC 9 cut(s) 17, 20, 68, 273, 287, 411, 440, 544, 755
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
PscI ACATGT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 572
PspGI CCWGG 1 cut(s) 572
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 4 cut(s) 719, 728, 752, 794
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 2 cut(s) 419, 717
PsyI GACNNNGTC 2 cut(s) 263, 298
PvuI CGATCG 1 cut(s) 189
PvuII CAGCTG 3 cut(s) 274, 438, 860
RsaI GTAC 2 cut(s) 257, 491
RsaNI GTAC 2 cut(s) 256, 490
RseI CAYNNNNRTG 1 cut(s) 590
SalI GTCGAC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 89
SatI GCNGC 9 cut(s) 16, 19, 67, 272, 286, 410, 439, 543, 754
Sau3AI GATC 5 cut(s) 186, 419, 692, 717, 735
Sau96I GGNCC 1 cut(s) 105
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 1 cut(s) 711
SfuI TTCGAA 1 cut(s) 672
SinI GGWCC 1 cut(s) 105
SmiMI CAYNNNNRTG 1 cut(s) 590
SphI GCATGC 1 cut(s) 555
Sse9I AATT 1 cut(s) 797
SsiI CCGC 7 cut(s) 5, 15, 200, 285, 405, 543, 754
SspI AATATT 1 cut(s) 529
StyD4I CCNGG 1 cut(s) 572
TaaI ACNGT 2 cut(s) 388, 592
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 129, 672
TasI AATT 1 cut(s) 797
TauI GCSGC 4 cut(s) 18, 288, 545, 756
TfiI GAWTC 1 cut(s) 511
TscAI CASTG 2 cut(s) 702, 835
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 5 cut(s) 18, 66, 271, 409, 438
Tsp45I GTSAC 1 cut(s) 784
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 2 cut(s) 702, 835
Tth111I GACNNNGTC 2 cut(s) 263, 298
Van91I CCANNNNNTGG 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 105
XceI RCATGY 2 cut(s) 58, 555
XmiI GTMKAC 3 cut(s) 129, 216, 474
XmnI GAANNNNTTC 2 cut(s) 56, 98
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.